Restriction map for
pFN21AE1857
(Circular) (Six-base) MAPSORT of: pFN21AE1857.seq Check: 2866 from: 1 to: 5839
pFN21AE1857 5839 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2041 3046 2041
Size: 1005 4834
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3649 278
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3182 1937
Size: 1245 4594
Acc65I G'GTAC_C
Cuts at: 3160 3160
Size: 5839
AclI AA'CG_TT
Cuts at: 1563 3966 4339 1563
Size: 2403 373 3063
Fragments arranged by size:
3063 2403 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2043 3902 5286 5554 1632
Size: 411 1859 1384 268 1917
Fragments arranged by size:
1917 1859 1384 411 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5642
AflIII A'CryG_T
Cuts at: 1203 4738 5715 1203
Size: 3535 977 1327
Fragments arranged by size:
3535 1327 977
AgeI A'CCGG_T
Cuts at: 4670 4670
Size: 5839
AhdI GACnn_n'nnGTC
Cuts at: 4568 4568
Size: 5839
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5839
AlwNI CAG_nnn'CTG
Cuts at: 3037 3582 5154 3037
Size: 545 1572 3722
Fragments arranged by size:
3722 1572 545
ApaLI G'TGCA_C
Cuts at: 2028 3896 5052 2028
Size: 1868 1156 2815
Fragments arranged by size:
2815 1868 1156
ApoI r'AATT_y
Cuts at: 1493 2300 2511 2677 2938 3148 3374 3526 5635
Size: 807 211 166 261 210 226 152 2109
Cuts at: 5635 1493
Size: 1697
Fragments arranged by size:
2109 1697 807 261 226 211 210 166
152
AseI AT'TA_AT
Cuts at: 160 1032 4393 160
Size: 872 3361 1606
Fragments arranged by size:
3361 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
AvaI C'yCGr_G
Cuts at: 1870 1943 3164 1870
Size: 73 1221 4545
Fragments arranged by size:
4545 1221 73
BamHI G'GATC_C
Cuts at: 2534 3169 3536 2534
Size: 635 367 4837
Fragments arranged by size:
4837 635 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2314 3160 4615 618
Size: 325 217 627 527 846 1455 1842
Fragments arranged by size:
1842 1455 846 627 527 325 217
BanII G_rGCy'C
Cuts at: 1391 3158 1391
Size: 1767 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2089 928
Size: 971 190 4678
Fragments arranged by size:
4678 971 190
BbvCI CC'TCA_GC
Cuts at: 2539 2539
Size: 5839
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4030 4064 1903
Size: 34 2093 34 3678
Fragments arranged by size:
3678 2093 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2189 3731 4941 2189
Size: 1542 1210 3087
Fragments arranged by size:
3087 1542 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5839
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2150 4450 136
Size: 107 122 71 631 605 478 2300 1525
Fragments arranged by size:
2300 1525 631 605 478 122 107 71
BglII A'GATC_T
Cuts at: 5834 5834
Size: 5839
BlpI GC'TnA_GC
Cuts at: 2837 2901 3256 2837
Size: 64 355 5420
Fragments arranged by size:
5420 355 64
Bme1580I G_kGCm'C
Cuts at: 1790 2032 2754 3900 5056 1790
Size: 242 722 1146 1156 2573
Fragments arranged by size:
2573 1156 1146 722 242
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5839
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2741 3570 4528
Size: 416 53 83 399 24 1324 829 958
Cuts at: 4528 442
Size: 1753
Fragments arranged by size:
1753 1324 958 829 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5839
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2579 4499 1239
Size: 1340 1920 2579
Fragments arranged by size:
2579 1920 1340
Bpu10I CC'TnA_GC
Cuts at: 2307 2539 2307
Size: 232 5607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3964 4829 5127 3964
Size: 865 298 4676
Fragments arranged by size:
4676 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4502 882
Size: 617 3003 2219
Fragments arranged by size:
3003 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2947 3630 493
Size: 711 1743 683 2702
Fragments arranged by size:
2702 1743 711 683
BsaBI GATnn'nnATC
Cuts at: 5833 5833
Size: 5839
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3646 4028 4663 275
Size: 53 83 186 936 2113 382 635 1451
Fragments arranged by size:
2113 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4272 4670 4944 5091 5502 4272
Size: 398 274 147 411 4609
Fragments arranged by size:
4609 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5614
Fragments arranged by size:
5614 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2492 1186
Size: 1306 4533
BseYI C'CCAG_C
Cuts at: 5042 5538 5042
Size: 496 5343
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5266
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2887 3289 4050 5078 1540
Size: 275 186 886 402 761 1028 2301
Fragments arranged by size:
2301 1028 886 761 402 275 186
BsiHKAI G_wGCw'C
Cuts at: 2032 3900 3985 5056 2032
Size: 1868 85 1071 2815
Fragments arranged by size:
2815 1868 1071 85
BsmI GAATG_Cn'
Cuts at: 3350 3443 3350
Size: 93 5746
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2032 2754 3158 3900 3985 5056 1391
Size: 399 242 722 404 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 722 404 399 242 85
BspEI T'CCGG_A
Cuts at: 5502 5502
Size: 5839
BspHI T'CATG_A
Cuts at: 3728 5458 3728
Size: 1730 4109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2041 3046 3194 844
Size: 1197 1005 148 3489
Fragments arranged by size:
3489 1197 1005 148
BsrBI CCG'CTC
Cuts at: 1394 1823 3726 1394
Size: 429 1903 3507
Fragments arranged by size:
3507 1903 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4334 4508 65
Size: 1183 3086 174 1396
Fragments arranged by size:
3086 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2070 4483 4670 1700
Size: 110 199 61 2413 187 2869
Fragments arranged by size:
2869 2413 199 187 110 61
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5839
BssHII G'CGCG_C
Cuts at: 1925 2090 1925
Size: 165 5674
BssSI C'ACGA_G
Cuts at: 3899 4911 3899
Size: 1012 4827
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3111 794
Size: 2317 3522
BstBI TT'CG_AA
Cuts at: 2438 4643 2438
Size: 2205 3634
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5839
BstXI CCAn_nnnn'nTGG
Cuts at: 2264 2264
Size: 5839
BstYI r'GATC_y
Cuts at: 1972 2083 2534 3169 3536 3921 3938 5390 5476
Size: 111 451 635 367 385 17 1452 86
Cuts at: 5476 5488 5834 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 635 451 385 367 346 111
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2145 513
Size: 552 939 141 4207
Fragments arranged by size:
4207 939 552 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5100
Fragments arranged by size:
5100 683 56
BtsI GCAGTG_nn'
Cuts at: 3009 3355 4149 4169 3009
Size: 346 794 20 4679
Fragments arranged by size:
4679 794 346 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5839
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5804
DraI TTT'AAA
Cuts at: 2914 3143 2914
Size: 229 5610
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4846 809
Size: 507 3530 1802
Fragments arranged by size:
3530 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2884 3286
Size: 54 1425 50 275 18 177 877 402
Cuts at: 3286 4175 5804 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 877 402 275 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2884 3286 1537
Size: 275 1072 402 4090
Fragments arranged by size:
4090 1072 402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2130 2435 2727 2732 3769 1353
Size: 777 305 292 5 1037 3423
Fragments arranged by size:
3423 1037 777 305 292 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2168 4426 4800 4946 387
Size: 1064 138 579 2258 374 146 1280
Fragments arranged by size:
2258 1280 1064 579 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2043 2579 3902 4499 5286 5554 1239
Size: 393 411 536 1323 597 787 268 1524
Fragments arranged by size:
1524 1323 787 597 536 411 393 268
EcoRI G'AATT_C
Cuts at: 2300 3148 3526 2300
Size: 848 378 4613
Fragments arranged by size:
4613 848 378
FspI TGC'GCA
Cuts at: 4345 4345
Size: 5839
HaeII r_GCGC'y
Cuts at: 1678 4986 1678
Size: 3308 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4480 4512 4554 4586 1373
Size: 32 135 32 2908 32 42 32 2626
Fragments arranged by size:
2908 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3183 3429 669
Size: 988 281 1245 246 3079
Fragments arranged by size:
3079 1245 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5839
HpaI GTT'AAC
Cuts at: 3429 3429
Size: 5839
KpnI G_GTAC'C
Cuts at: 3164 3164
Size: 5839
MfeI C'AATT_G
Cuts at: 2799 3438 2799
Size: 639 5200
MluI A'CGCG_T
Cuts at: 5715 5715
Size: 5839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2112 4953 5137 1305
Size: 807 2841 184 2007
Fragments arranged by size:
2841 2007 807 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4017
Fragments arranged by size:
4017 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2106 2262 3797 4156 4315 518
Size: 648 940 156 1535 359 159 2042
Fragments arranged by size:
2042 1535 940 648 359 159 156
MspA1I CmG'CkG
Cuts at: 1606 2147 2185 3255 3932 5080 5325 1606
Size: 541 38 1070 677 1148 245 2120
Fragments arranged by size:
2120 1148 1070 677 541 245 38
NaeI GCC'GGC
Cuts at: 2011 2011
Size: 5839
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4348
Fragments arranged by size:
4348 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5839
NgoMIV G'CCGG_C
Cuts at: 2009 2009
Size: 5839
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5839
NotI GC'GGCC_GC
Cuts at: 3286 3286
Size: 5839
NspI r_CATG'y
Cuts at: 1132 1229 3197 4742 1132
Size: 97 1968 1545 2229
Fragments arranged by size:
2229 1968 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5839
PciI A'CATG_T
Cuts at: 4738 4738
Size: 5839
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5839
PfoI T'CCnGG_A
Cuts at: 2166 2530 2166
Size: 364 5475
PmeI GTTT'AAAC
Cuts at: 3143 3143
Size: 5839
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3908 3940 5421 5453 3908
Size: 32 1481 32 4294
Fragments arranged by size:
4294 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5839
PsiI TTA'TAA
Cuts at: 3409 3409
Size: 5839
PstI C_TGCA'G
Cuts at: 830 2038 3057 3191 830
Size: 1208 1019 134 3478
Fragments arranged by size:
3478 1208 1019 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5839
SacII CC_GC'GG
Cuts at: 2148 2148
Size: 5839
SalI G'TCGA_C
Cuts at: 1936 3181 1936
Size: 1245 4594
SapI GCTCTTCn'nnn_
Cuts at: 2130 2435 2130
Size: 305 5534
SbfI CC_TGCA'GG
Cuts at: 3191 3191
Size: 5839
ScaI AGT'ACT
Cuts at: 4087 5799 4087
Size: 1712 4127
SexAI A'CCwGG_T
Cuts at: 2401 3036 2401
Size: 635 5204
SfcI C'TryA_G
Cuts at: 826 1045 2034 2658 3053 3187 4322 5003 5194
Size: 219 989 624 395 134 1135 681 191
Cuts at: 5194 826
Size: 1471
Fragments arranged by size:
1471 1135 989 681 624 395 219 191
134
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5839
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5839
SmaI CCC'GGG
Cuts at: 1872 3166 1872
Size: 1294 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3943 4844 5106 820
Size: 197 926 2000 901 262 1553
Fragments arranged by size:
2000 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3630 493
Size: 3137 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5839
SphI G_CATG'C
Cuts at: 1132 3197 1132
Size: 2065 3774
SspI AAT'ATT
Cuts at: 5 52 3763 5
Size: 47 3711 2081
Fragments arranged by size:
3711 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5839
StyI C'CwwG_G
Cuts at: 513 1065 2004 2754 3278 513
Size: 552 939 750 524 3074
Fragments arranged by size:
3074 939 750 552 524
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3866 3883 4036 4221 4666 325
Size: 1255 2286 17 153 185 445 1498
Fragments arranged by size:
2286 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4085 5797 96
Size: 274 80 33 51 1443 2108 1712 138
Fragments arranged by size:
2108 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2022 2054 2575 2607 5799 5831 2022
Size: 32 521 32 3192 32 2030
Fragments arranged by size:
3192 2030 521 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5839
XbaI T'CTAG_A
Cuts at: 3175 3175
Size: 5839
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3016 3016
Size: 5839
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5839
XmaI C'CCGG_G
Cuts at: 1870 3164 1870
Size: 1294 4545
XmnI GAAnn'nnTTC
Cuts at: 2243 3027 3968 2243
Size: 784 941 4114
Fragments arranged by size:
4114 941 784
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3647 276
Size: 53 83 186 936 2113 2468
Fragments arranged by size:
2468 2113 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI SacII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SanDI SfiI SfoI SrfI SwaI