Restriction map for
pFN21AE1857
(Linear) (Six-base) MAPSORT of: KIEE1857.seq Check: 5982 from: 1 to: 1134
KIEE1857.orf
With 182 enzymes: SgfI *
July 15, 2010 18:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 36 1041 1134
Size: 36 1005 93
Fragments arranged by size:
1005 93 36
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 1134
Size: 38 1096
AlwNI CAG_nnn'CTG
Cuts at: 0 1032 1134
Size: 1032 102
ApaLI G'TGCA_C
Cuts at: 0 23 1134
Size: 23 1111
ApoI r'AATT_y
Cuts at: 0 295 506 672 933 1134
Size: 295 211 166 261 201
Fragments arranged by size:
295 261 211 201 166
BamHI G'GATC_C
Cuts at: 0 529 1134
Size: 529 605
BanI G'GyrC_C
Cuts at: 0 309 1134
Size: 309 825
BbsI GAAGACnn'nnnn_
Cuts at: 0 84 1134
Size: 84 1050
BbvCI CC'TCA_GC
Cuts at: 0 534 1134
Size: 534 600
BciVI GTATCCnnnnn_n'
Cuts at: 0 184 1134
Size: 184 950
BglI GCCn_nnn'nGGC
Cuts at: 0 145 1134
Size: 145 989
BlpI GC'TnA_GC
Cuts at: 0 832 896 1134
Size: 832 64 238
Fragments arranged by size:
832 238 64
Bme1580I G_kGCm'C
Cuts at: 0 27 749 1134
Size: 27 722 385
Fragments arranged by size:
722 385 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 736 1134
Size: 736 398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 1134
Size: 574 560
Bpu10I CC'TnA_GC
Cuts at: 0 302 534 1134
Size: 302 232 600
Fragments arranged by size:
600 302 232
BsaAI yAC'GTr
Cuts at: 0 942 1134
Size: 942 192
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 487 1134
Size: 487 647
BsiEI CG_ry'CG
Cuts at: 0 882 1134
Size: 882 252
BsiHKAI G_wGCw'C
Cuts at: 0 27 1134
Size: 27 1107
Bsp1286I G_dGCh'C
Cuts at: 0 27 749 1134
Size: 27 722 385
Fragments arranged by size:
722 385 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 36 1041 1134
Size: 36 1005 93
Fragments arranged by size:
1005 93 36
BsrFI r'CCGG_y
Cuts at: 0 4 65 1134
Size: 4 61 1069
Fragments arranged by size:
1069 61 4
BssHII G'CGCG_C
Cuts at: 0 85 1134
Size: 85 1049
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1106 1134
Size: 1106 28
BstBI TT'CG_AA
Cuts at: 0 433 1134
Size: 433 701
BstXI CCAn_nnnn'nTGG
Cuts at: 0 259 1134
Size: 259 875
BstYI r'GATC_y
Cuts at: 0 78 529 1134
Size: 78 451 605
Fragments arranged by size:
605 451 78
BtgI C'CryG_G
Cuts at: 0 140 1134
Size: 140 994
BtsI GCAGTG_nn'
Cuts at: 0 1004 1134
Size: 1004 130
DraI TTT'AAA
Cuts at: 0 909 1134
Size: 909 225
EaeI y'GGCC_r
Cuts at: 0 2 879 1134
Size: 2 877 255
Fragments arranged by size:
877 255 2
EagI C'GGCC_G
Cuts at: 0 879 1134
Size: 879 255
EarI CTCTTCn'nnn_
Cuts at: 0 125 430 722 727 1134
Size: 125 305 292 5 407
Fragments arranged by size:
407 305 292 125 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 163 1134
Size: 163 971
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 574 1134
Size: 38 536 560
Fragments arranged by size:
560 536 38
EcoRI G'AATT_C
Cuts at: 0 295 1134
Size: 295 839
MfeI C'AATT_G
Cuts at: 0 794 1134
Size: 794 340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 1134
Size: 107 1027
MslI CAynn'nnrTG
Cuts at: 0 101 257 1134
Size: 101 156 877
Fragments arranged by size:
877 156 101
MspA1I CmG'CkG
Cuts at: 0 142 180 1134
Size: 142 38 954
Fragments arranged by size:
954 142 38
NaeI GCC'GGC
Cuts at: 0 6 1134
Size: 6 1128
NgoMIV G'CCGG_C
Cuts at: 0 4 1134
Size: 4 1130
PfoI T'CCnGG_A
Cuts at: 0 161 525 1134
Size: 161 364 609
Fragments arranged by size:
609 364 161
PstI C_TGCA'G
Cuts at: 0 33 1052 1134
Size: 33 1019 82
Fragments arranged by size:
1019 82 33
SacII CC_GC'GG
Cuts at: 0 143 1134
Size: 143 991
SapI GCTCTTCn'nnn_
Cuts at: 0 125 430 1134
Size: 125 305 704
Fragments arranged by size:
704 305 125
SexAI A'CCwGG_T
Cuts at: 0 396 1031 1134
Size: 396 635 103
Fragments arranged by size:
635 396 103
SfcI C'TryA_G
Cuts at: 0 29 653 1048 1134
Size: 29 624 395 86
Fragments arranged by size:
624 395 86 29
StyI C'CwwG_G
Cuts at: 0 749 1134
Size: 749 385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 570 602 1134
Size: 17 32 521 32 532
Fragments arranged by size:
532 521 32 32 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1011 1134
Size: 1011 123
XmnI GAAnn'nnTTC
Cuts at: 0 238 1022 1134
Size: 238 784 112
Fragments arranged by size:
784 238 112
Enzymes that do cut:
AarI AcuI AlwNI ApaLI ApoI BamHI BanI BbsI
BbvCI BciVI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BsaAI BseRI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII
BstAPI BstBI BstXI BstYI BtgI BtsI DraI EaeI
EagI EarI EciI Eco57MI EcoRI MfeI MmeI MslI
MspA1I NaeI NgoMIV PfoI PstI SacII SapI SexAI
SfcI StyI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanII BbeI BcgI BclI BfrBI
BglII BmgBI BmtI BplI BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssSI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MscI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XhoI XmaI ZraI