Restriction map for
pFN21AE4011
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# NO CUT ENZYMES ( 194 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbsI BcgI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DpnII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeI MamI MboI MfeI MflI
MluI MluNI MroI MscI MslI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TspRI
Tth111I TthHB8I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 32 GTCTAC GT^[AC][GT]AC
AcsI 334 GAATTT [GA]^AATT[CT]
AlwNI 395 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 334 GAATTT [GA]^AATT[CT]
BanI 259 GGTGCC G^G[CT][GA]CC
BbrPI 328 CACGTG CAC^GTG
BbvCI 468 CCTCAGC CCTCAGC(-5/-2)
BciVI 75 GGATAC GTATCC(6/5)
BsaAI 328 CACGTG [CT]AC^GT[GA]
BsgI 392 GTGCAG GTGCAG(16/14)
BspMI 386 GCAGGT ACCTGC(4/8)
BsrI 432 CCAGT ACTGG(1/-1)
DdeI 469 CTCAG C^T[ACGT]AG
DraI 25 TTTAAA TTT^AAA
DraII 464 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 451 CCGTGG C^C[GA][CT]GG
EcoNI 139 CCTACAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 464 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 302 GCGGG CCCGC(4/6)
HphI 436 TCACC GGTGA(8/7)
MaeII 329 ACGT A^CGT
MboII 499 TCTTC GAAGA(8/7)
MseI 26 TTAA T^TAA
MwoI 269 GCCCAGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 503 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 415 GACTC GAGTC(4/5)
PmaCI 328 CACGTG CAC^GTG
PmlI 328 CACGTG CAC^GTG
PpuMI 464 AGGACCT [GA]G^G[AT]CC[CT]
SfaNI 51 GATGC GCATC(5/9)
SfcI 140 CTACAG C^T[GA][CT]AG
TfiI 57 GATTC G^A[AT]TC
Tru9I 26 TTAA T^TAA
Van91I 503 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaII 448 GGACC G^G[AT]CC
465 GGACC
BsmAI 121 GAGAC GTCTC(1/5)
160 GAGAC
BsmFI 447 GGGAC GGGAC(10/14)
136 GTCCC
BssKI 271 CCAGG ^CC[ACGT]GG
443 CCTGG
BstNI 271 CCAGG CC^[AT]GG
443 CCTGG
Cfr13I 448 GGACC G^G[ACGT]CC
465 GGACC
EcoRII 271 CCAGG ^CC[AT]GG
443 CCTGG
HinfI 57 GATTC G^A[ACGT]TC
415 GACTC
MaeIII 326 GTCAC ^GT[ACGT]AC
435 GTCAC
MvaI 271 CCAGG CC^[AT]GG
443 CCTGG
NlaIV 259 GGTGCC GG[ACGT]^[ACGT]CC
447 GGGACC
Sau96I 448 GGACC G^G[ACGT]CC
465 GGACC
ScrFI 271 CCAGG CC^[ACGT]GG
443 CCTGG
Tsp45I 326 GTCAC ^GT[GC]AC
435 GTCAC
Tsp509I 107 AATT ^AATT
335 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BstF5I Cac8I Fnu4HI FokI
ItaI MnlI MspA1I PvuII TseI