Restriction map for
pFN21AE4072
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# NO CUT ENZYMES ( 126 enzymes) #
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Acc65I AccI AccIII AclI AfaI
AflII AflIII AgeI AhdI AlwNI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
HphI KpnI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NcoI NdeI NheI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuII RcaI RsaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfcI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp45I Tth111I Van91I XbaI
XcmI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 325 GACGTC GACGT^C
Alw44I 401 GTGCAC G^TGCAC
ApaLI 401 GTGCAC G^TGCAC
Asp700I 774 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 985 GAAGAC GAAGAC(2/6)
BbvCI 496 CCTCAGC CCTCAGC(-5/-2)
BglII 714 AGATCT A^GATCT
BpuAI 985 GAAGAC GAAGAC(2/6)
BsgI 908 CTGCAC GTGCAG(16/14)
BspMI 555 ACCTGC ACCTGC(4/8)
BsrBI 817 GAGCGG CCGCTC(-3/-3)
BssSI 927 CACGAG CACGAG(-5/-1)
BstYI 714 AGATCT [GA]^GATC[CT]
EarI 358 GAAGAG CTCTTC(1/4)
EcoNI 826 CCTGCGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 100 GAATTC G^AATTC
EcoT14I 785 CCTTGG C^C[AT][AT]GG
Ksp632I 358 GAAGAG CTCTTC(1/4)
MaeII 326 ACGT A^CGT
MaeIII 767 GTTAC ^GT[ACGT]AC
MflI 714 AGATCT [GA]^GATC[CT]
NlaIII 1125 CATG CATG^
NotI 819 GCGGCCGC GC^GGCCGC
PaeR7I 490 CTCGAG C^TCGAG
PleI 1030 GACTC GAGTC(4/5)
PvuI 735 CGATCG CGAT^CG
SfiI 812 GGCCAGAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 309 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 490 CTCGAG C^T[CT][GA]AG
StyI 785 CCTTGG C^C[AT][AT]GG
TfiI 442 GAATC G^A[AT]TC
XhoI 490 CTCGAG C^TCGAG
XhoII 714 AGATCT [GA]^GATC[CT]
XmnI 774 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 100 GAATTC [GA]^AATT[CT]
1108 AAATTC
AluI 185 AGCT AG^CT
454 AGCT
AlwI 744 GATCC GGATC(4/5)
1118 GGATC
AocI 217 CCTCAGG CC^T[ACGT]AGG
523 CCTCAGG
ApoI 100 GAATTC [GA]^AATT[CT]
1108 AAATTC
AspHI 401 GTGCAC G[AT]GC[AT]^C
930 GAGCAC
BbeI 594 GGCGCC GGCGC^C
873 GGCGCC
BpmI 665 CTCCAG CTGGAG(16/14)
1021 CTCCAG
BseRI 943 CTCCTC GAGGAG(10/8)
352 GAGGAG
BsiHKAI 401 GTGCAC G[AT]GC[AT]^C
930 GAGCAC
BsrI 202 CCAGT ACTGG(1/-1)
533 CCAGT
Bsu36I 217 CCTCAGG CC^T[ACGT]AGG
523 CCTCAGG
Eco81I 217 CCTCAGG CC^T[ACGT]AGG
523 CCTCAGG
HaeII 594 GGCGCC [GA]GCGC^[CT]
873 GGCGCC
HinfI 442 GAATC G^A[ACGT]TC
1030 GACTC
KasI 594 GGCGCC G^GCGCC
873 GGCGCC
MboII 358 GAAGA GAAGA(8/7)
985 GAAGA
NarI 594 GGCGCC GG^CGCC
873 GGCGCC
SfaNI 319 GATGC GCATC(5/9)
1006 GCATC
SfoI 594 GGCGCC GGC^GCC
873 GGCGCC
Tsp509I 101 AATT ^AATT
1109 AATT
TspRI 32 CAGTG CA[GC]TG[ACGT][ACGT]^
203 CAGTG
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BglI BmyI BsaHI BsaI BsaJI
BsiEI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII ItaI KspI MboI MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIV
PpuMI SacII Sau3AI Sau96I ScrFI
SmaI TaqI TseI TthHB8I XmaCI
XmaI