(Circular) (Six-base) MAPSORT of: pFN21AE4072.seq Check: 8452 from: 1 to: 5842 pFN21AE4072 5842 bp With 182 enzymes: SgfI * March 28, 2012 13:04 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2569 2569 Size: 5842 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2334 3652 278 Size: 53 83 186 936 798 1318 2468 Fragments arranged by size: 2468 1318 936 798 186 83 53 AccI GT'mk_AC Cuts at: 1937 3185 1937 Size: 1248 4594 Acc65I G'GTAC_C Cuts at: 3163 3163 Size: 5842 AclI AA'CG_TT Cuts at: 1563 3969 4342 1563 Size: 2406 373 3063 Fragments arranged by size: 3063 2406 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2285 2926 3905 5289 5557 1632 Size: 653 641 979 1384 268 1917 Fragments arranged by size: 1917 1384 979 653 641 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5645 AflIII A'CryG_T Cuts at: 1203 4741 5718 1203 Size: 3538 977 1327 Fragments arranged by size: 3538 1327 977 AgeI A'CCGG_T Cuts at: 4673 4673 Size: 5842 AhdI GACnn_n'nnGTC Cuts at: 4571 4571 Size: 5842 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5842 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5842 AlwNI CAG_nnn'CTG Cuts at: 3585 5157 3585 Size: 1572 4270 ApaI G_GGCC'C Cuts at: 2177 2222 2254 2177 Size: 45 32 5765 Fragments arranged by size: 5765 45 32 ApaLI G'TGCA_C Cuts at: 2406 3899 5055 2406 Size: 1493 1156 3193 Fragments arranged by size: 3193 1493 1156 ApoI r'AATT_y Cuts at: 1493 2105 3113 3151 3377 3529 5638 1493 Size: 612 1008 38 226 152 2109 1697 Fragments arranged by size: 2109 1697 1008 612 226 152 38 AseI AT'TA_AT Cuts at: 160 1032 4396 160 Size: 872 3364 1606 Fragments arranged by size: 3364 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 AvaI C'yCGr_G Cuts at: 1870 1943 2157 2176 2442 2488 2495 2591 2663 Size: 73 214 19 266 46 7 96 72 Cuts at: 2663 2847 3167 1870 Size: 184 320 4545 Fragments arranged by size: 4545 320 266 214 184 96 73 72 46 19 7 BamHI G'GATC_C Cuts at: 3172 3539 3172 Size: 367 5475 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2278 2599 2878 3163 4618 Size: 325 217 627 491 321 279 285 1455 Cuts at: 4618 618 Size: 1842 Fragments arranged by size: 1842 1455 627 491 325 321 285 279 217 BanII G_rGCy'C Cuts at: 1391 2177 2222 2254 2583 3102 3161 1391 Size: 786 45 32 329 519 59 4072 Fragments arranged by size: 4072 786 519 329 59 45 32 BbeI G_GCGC'C Cuts at: 2603 2882 2603 Size: 279 5563 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2997 928 Size: 971 1098 3773 Fragments arranged by size: 3773 1098 971 BbvCI CC'TCA_GC Cuts at: 2502 2502 Size: 5842 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4033 4067 1903 Size: 34 2096 34 3678 Fragments arranged by size: 3678 2096 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3734 4944 3734 Size: 1210 4632 BclI T'GATC_A Cuts at: 1548 1548 Size: 5842 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2244 2666 2699 Size: 107 122 71 631 605 572 422 33 Cuts at: 2699 2824 4453 136 Size: 125 1629 1525 Fragments arranged by size: 1629 1525 631 605 572 422 125 122 107 71 33 BglII A'GATC_T Cuts at: 2719 5837 2719 Size: 3118 2724 BlpI GC'TnA_GC Cuts at: 3259 3259 Size: 5842 Bme1580I G_kGCm'C Cuts at: 1790 2021 2131 2177 2222 2254 2281 2345 2410 Size: 231 110 46 45 32 27 64 65 Cuts at: 2410 3903 5059 1790 Size: 1493 1156 2573 Fragments arranged by size: 2573 1493 1156 231 110 65 64 46 45 32 27 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5842 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2201 2532 3573 Size: 416 53 83 399 24 784 331 1041 Cuts at: 3573 4531 442 Size: 958 1753 Fragments arranged by size: 1753 1041 958 784 416 399 331 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5842 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2655 3011 4502 1239 Size: 1416 356 1491 2579 Fragments arranged by size: 2579 1491 1416 356 Bpu10I CC'TnA_GC Cuts at: 2502 3096 2502 Size: 594 5248 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3967 4832 5130 3967 Size: 865 298 4679 Fragments arranged by size: 4679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 2498 2991 4505 882 Size: 617 504 495 493 1514 2219 Fragments arranged by size: 2219 1514 617 504 495 493 BsaAI yAC'GTr Cuts at: 493 1204 3633 493 Size: 711 2429 2702 Fragments arranged by size: 2702 2429 711 BsaBI GATnn'nnATC Cuts at: 5836 5836 Size: 5842 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2045 2162 2331 2600 Size: 53 83 186 936 512 117 169 269 Cuts at: 2600 2879 3649 4031 4666 275 Size: 279 770 382 635 1451 Fragments arranged by size: 1451 936 770 635 512 382 279 269 186 169 117 83 53 BsaWI w'CCGG_w Cuts at: 4275 4673 4947 5094 5505 4275 Size: 398 274 147 411 4612 Fragments arranged by size: 4612 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2907 2937 1178 Size: 30 165 30 597 30 877 30 4083 Fragments arranged by size: 4083 877 597 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2372 2939 1186 Size: 1186 567 4089 Fragments arranged by size: 4089 1186 567 BseYI C'CCAG_C Cuts at: 2247 2512 5045 5541 2247 Size: 265 2533 496 2548 Fragments arranged by size: 2548 2533 496 265 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2898 1163 Size: 573 1162 4107 Fragments arranged by size: 4107 1162 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2286 2609 2658 2709 2743 2759 Size: 275 186 285 323 49 51 34 16 Cuts at: 2759 2828 3292 4053 5081 1540 Size: 69 464 761 1028 2301 Fragments arranged by size: 2301 1028 761 464 323 285 275 186 69 51 49 34 16 BsiHKAI G_wGCw'C Cuts at: 2410 2939 3903 3988 5059 2410 Size: 529 964 85 1071 3193 Fragments arranged by size: 3193 1071 964 529 85 BsmI GAATG_Cn' Cuts at: 3353 3446 3353 Size: 93 5749 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2131 2177 2222 2254 2281 2345 Size: 399 231 110 46 45 32 27 64 Cuts at: 2345 2410 2583 2939 3102 3161 3903 3988 5059 Size: 65 173 356 163 59 742 85 1071 Cuts at: 5059 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 356 231 173 163 110 85 65 64 59 46 45 32 27 BspEI T'CCGG_A Cuts at: 5505 5505 Size: 5842 BspHI T'CATG_A Cuts at: 3731 5461 3731 Size: 1730 4112 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2569 3197 844 Size: 1725 628 3489 Fragments arranged by size: 3489 1725 628 BsrBI CCG'CTC Cuts at: 1394 1823 2824 3729 1394 Size: 429 1001 905 3507 Fragments arranged by size: 3507 1001 905 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4337 4511 65 Size: 1183 3089 174 1396 Fragments arranged by size: 3089 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2041 2315 2653 2754 2842 2868 2875 Size: 110 231 274 338 101 88 26 7 Cuts at: 2875 3007 4486 4673 1700 Size: 132 1479 187 2869 Fragments arranged by size: 2869 1479 338 274 231 187 132 110 101 88 26 7 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5842 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5842 BssSI C'ACGA_G Cuts at: 2932 3902 4914 2932 Size: 970 1012 3860 Fragments arranged by size: 3860 1012 970 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5842 BstBI TT'CG_AA Cuts at: 4646 4646 Size: 5842 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5842 BstYI r'GATC_y Cuts at: 1972 2719 3172 3539 3924 3941 5393 5479 5491 Size: 747 453 367 385 17 1452 86 12 Cuts at: 5491 5837 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 747 453 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2223 2529 2223 Size: 306 5536 BtgI C'CryG_G Cuts at: 513 1065 2004 2350 2583 2603 513 Size: 552 939 346 233 20 3752 Fragments arranged by size: 3752 939 552 346 233 20 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5103 Fragments arranged by size: 5103 683 56 BtsI GCAGTG_nn' Cuts at: 2043 3358 4152 4172 2043 Size: 1315 794 20 3713 Fragments arranged by size: 3713 1315 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5842 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5807 DraI TTT'AAA Cuts at: 3146 3146 Size: 5842 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4849 809 Size: 507 3533 1802 Fragments arranged by size: 3533 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2606 2620 2655 Size: 54 1425 50 275 18 776 14 35 Cuts at: 2655 2676 2691 2706 2825 3289 4178 5807 8 Size: 21 15 15 119 464 889 1629 43 Fragments arranged by size: 1629 1425 889 776 464 275 119 54 50 43 35 21 18 15 15 14 EagI C'GGCC_G Cuts at: 1537 1812 2606 2655 2706 2825 3289 1537 Size: 275 794 49 51 119 464 4090 Fragments arranged by size: 4090 794 464 275 119 51 49 EarI CTCTTCn'nnn_ Cuts at: 1353 2358 3772 1353 Size: 1005 1414 3423 Fragments arranged by size: 3423 1414 1005 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2385 2714 2741 4429 4803 4949 Size: 1064 138 796 329 27 1688 374 146 Cuts at: 4949 387 Size: 1280 Fragments arranged by size: 1688 1280 1064 796 374 329 146 138 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2655 2926 3011 3905 4502 5289 Size: 393 653 370 271 85 894 597 787 Cuts at: 5289 5557 1239 Size: 268 1524 Fragments arranged by size: 1524 894 787 653 597 393 370 271 268 85 EcoNI CCTnn'n_nnAGG Cuts at: 2835 2835 Size: 5842 EcoO109I rG'GnC_Cy Cuts at: 2097 2180 2218 2219 2227 2251 2588 3083 2097 Size: 83 38 1 8 24 337 495 4856 Fragments arranged by size: 4856 495 337 83 38 24 8 1 EcoRI G'AATT_C Cuts at: 2105 3151 3529 2105 Size: 1046 378 4418 Fragments arranged by size: 4418 1046 378 FspI TGC'GCA Cuts at: 4348 4348 Size: 5842 HaeII r_GCGC'y Cuts at: 1678 2603 2882 4989 1678 Size: 925 279 2107 2531 Fragments arranged by size: 2531 2107 925 279 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3067 3099 4483 4515 4557 Size: 32 135 32 1495 32 1384 32 42 Cuts at: 4557 4589 1373 Size: 32 2626 Fragments arranged by size: 2626 1495 1384 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3186 3432 669 Size: 988 281 1248 246 3079 Fragments arranged by size: 3079 1248 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5842 HpaI GTT'AAC Cuts at: 3432 3432 Size: 5842 KasI G'GCGC_C Cuts at: 2599 2878 2599 Size: 279 5563 KpnI G_GTAC'C Cuts at: 3167 3167 Size: 5842 MfeI C'AATT_G Cuts at: 3441 3441 Size: 5842 MluI A'CGCG_T Cuts at: 5718 5718 Size: 5842 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4956 5140 1305 Size: 3651 184 2007 Fragments arranged by size: 3651 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4020 Fragments arranged by size: 4020 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3800 4159 4318 518 Size: 648 2634 359 159 2042 Fragments arranged by size: 2634 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2116 2352 2421 2585 2605 2675 3061 3089 Size: 510 236 69 164 20 70 386 28 Cuts at: 3089 3258 3935 5083 5328 1606 Size: 169 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 510 386 245 236 169 164 70 69 28 20 NaeI GCC'GGC Cuts at: 2043 2317 2655 2844 2870 2877 3009 2043 Size: 274 338 189 26 7 132 4876 Fragments arranged by size: 4876 338 274 189 132 26 7 NarI GG'CG_CC Cuts at: 2600 2879 2600 Size: 279 5563 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4351 Fragments arranged by size: 4351 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5842 NgoMIV G'CCGG_C Cuts at: 2041 2315 2653 2842 2868 2875 3007 2041 Size: 274 338 189 26 7 132 4876 Fragments arranged by size: 4876 338 274 189 132 26 7 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5842 NotI GC'GGCC_GC Cuts at: 2825 3289 2825 Size: 464 5378 NspI r_CATG'y Cuts at: 1132 1229 3200 4745 1132 Size: 97 1971 1545 2229 Fragments arranged by size: 2229 1971 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5842 PciI A'CATG_T Cuts at: 4741 4741 Size: 5842 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5842 PmeI GTTT'AAAC Cuts at: 3146 3146 Size: 5842 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3911 3943 5424 5456 3911 Size: 32 1481 32 4297 Fragments arranged by size: 4297 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2097 2588 3083 2097 Size: 491 495 4856 Fragments arranged by size: 4856 495 491 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5842 PsiI TTA'TAA Cuts at: 3412 3412 Size: 5842 PspOMI G'GGCC_C Cuts at: 2173 2218 2250 2173 Size: 45 32 5765 Fragments arranged by size: 5765 45 32 PstI C_TGCA'G Cuts at: 830 3194 830 Size: 2364 3478 PvuI CG_AT'CG Cuts at: 2001 2743 2001 Size: 742 5100 SacII CC_GC'GG Cuts at: 2353 2586 2606 2353 Size: 233 20 5589 Fragments arranged by size: 5589 233 20 SalI G'TCGA_C Cuts at: 1936 3184 1936 Size: 1248 4594 SanDI GG'GwC_CC Cuts at: 2097 2588 3083 2097 Size: 491 495 4856 Fragments arranged by size: 4856 495 491 SbfI CC_TGCA'GG Cuts at: 3194 3194 Size: 5842 ScaI AGT'ACT Cuts at: 4090 5802 4090 Size: 1712 4130 SfcI C'TryA_G Cuts at: 826 1045 3190 4325 5006 5197 826 Size: 219 2145 1135 681 191 1471 Fragments arranged by size: 2145 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2824 2824 Size: 5842 SfoI GGC'GCC Cuts at: 2601 2880 2601 Size: 279 5563 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5842 SgrAI Cr'CCGG_yG Cuts at: 1700 2315 1700 Size: 615 5227 SmaI CCC'GGG Cuts at: 1872 2159 2178 2490 2593 3169 1872 Size: 287 19 312 103 576 4545 Fragments arranged by size: 4545 576 312 287 103 19 SmlI C'TyrA_G Cuts at: 820 1017 1943 2495 3946 4847 5109 820 Size: 197 926 552 1451 901 262 1553 Fragments arranged by size: 1553 1451 926 901 552 262 197 SnaBI TAC'GTA Cuts at: 493 3633 493 Size: 3140 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5842 SphI G_CATG'C Cuts at: 1132 3200 1132 Size: 2068 3774 SspI AAT'ATT Cuts at: 5 52 3766 5 Size: 47 3714 2081 Fragments arranged by size: 3714 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5842 StyI C'CwwG_G Cuts at: 513 1065 2004 2790 3281 513 Size: 552 939 786 491 3074 Fragments arranged by size: 3074 939 786 552 491 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3869 3886 4039 4224 4669 325 Size: 1255 2289 17 153 185 445 1498 Fragments arranged by size: 2289 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4088 5800 96 Size: 274 80 33 51 1443 2111 1712 138 Fragments arranged by size: 2111 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2907 2939 5802 5834 2000 Size: 32 875 32 2863 32 2008 Fragments arranged by size: 2863 2008 875 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5842 XbaI T'CTAG_A Cuts at: 3178 3178 Size: 5842 XhoI C'TCGA_G Cuts at: 1943 2495 1943 Size: 552 5290 XmaI C'CCGG_G Cuts at: 1870 2157 2176 2488 2591 3167 1870 Size: 287 19 312 103 576 4545 Fragments arranged by size: 4545 576 312 287 103 19 XmnI GAAnn'nnTTC Cuts at: 2783 3971 2783 Size: 1188 4654 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2332 3650 276 Size: 53 83 186 936 798 1318 2468 Fragments arranged by size: 2468 1318 936 798 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SrfI SwaI XcmI