(Linear) (Six-base) MAPSORT of: KIEE4072.seq Check: 7142 from: 1 to: 1137 KIEE4072.orf With 182 enzymes: SgfI * March 22, 2012 17:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 564 1137 Size: 564 573 AatII G_ACGT'C Cuts at: 0 329 1137 Size: 329 808 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 921 1137 Size: 280 641 216 Fragments arranged by size: 641 280 216 ApaI G_GGCC'C Cuts at: 0 172 217 249 1137 Size: 172 45 32 888 Fragments arranged by size: 888 172 45 32 ApaLI G'TGCA_C Cuts at: 0 401 1137 Size: 401 736 ApoI r'AATT_y Cuts at: 0 100 1108 1137 Size: 100 1008 29 Fragments arranged by size: 1008 100 29 AvaI C'yCGr_G Cuts at: 0 152 171 437 483 490 586 658 842 Size: 152 19 266 46 7 96 72 184 Cuts at: 842 1137 Size: 295 Fragments arranged by size: 295 266 184 152 96 72 46 19 7 BanI G'GyrC_C Cuts at: 0 273 594 873 1137 Size: 273 321 279 264 Fragments arranged by size: 321 279 273 264 BanII G_rGCy'C Cuts at: 0 172 217 249 578 1097 1137 Size: 172 45 32 329 519 40 Fragments arranged by size: 519 329 172 45 40 32 BbeI G_GCGC'C Cuts at: 0 598 877 1137 Size: 598 279 260 Fragments arranged by size: 598 279 260 BbsI GAAGACnn'nnnn_ Cuts at: 0 992 1137 Size: 992 145 BbvCI CC'TCA_GC Cuts at: 0 497 1137 Size: 497 640 BglI GCCn_nnn'nGGC Cuts at: 0 239 661 694 819 1137 Size: 239 422 33 125 318 Fragments arranged by size: 422 318 239 125 33 BglII A'GATC_T Cuts at: 0 714 1137 Size: 714 423 Bme1580I G_kGCm'C Cuts at: 0 16 126 172 217 249 276 340 405 Size: 16 110 46 45 32 27 64 65 Cuts at: 405 1137 Size: 732 Fragments arranged by size: 732 110 65 64 46 45 32 27 16 BmrI ACTGGGnnnn_n' Cuts at: 0 196 527 1137 Size: 196 331 610 Fragments arranged by size: 610 331 196 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 650 1006 1137 Size: 650 356 131 Fragments arranged by size: 650 356 131 Bpu10I CC'TnA_GC Cuts at: 0 497 1091 1137 Size: 497 594 46 Fragments arranged by size: 594 497 46 BsaI GGTCTCn'nnnn_ Cuts at: 0 493 986 1137 Size: 493 493 151 Fragments arranged by size: 493 493 151 BsaHI Gr'CG_yC Cuts at: 0 40 157 326 595 874 1137 Size: 40 117 169 269 279 263 Fragments arranged by size: 279 269 263 169 117 40 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 25 902 932 1137 Size: 25 877 30 205 Fragments arranged by size: 877 205 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 367 934 1137 Size: 367 567 203 Fragments arranged by size: 567 367 203 BseYI C'CCAG_C Cuts at: 0 242 507 1137 Size: 242 265 630 Fragments arranged by size: 630 265 242 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 893 1137 Size: 893 244 BsiEI CG_ry'CG Cuts at: 0 281 604 653 704 738 754 823 1137 Size: 281 323 49 51 34 16 69 314 Fragments arranged by size: 323 314 281 69 51 49 34 16 BsiHKAI G_wGCw'C Cuts at: 0 405 934 1137 Size: 405 529 203 Fragments arranged by size: 529 405 203 Bsp1286I G_dGCh'C Cuts at: 0 16 126 172 217 249 276 340 405 Size: 16 110 46 45 32 27 64 65 Cuts at: 405 578 934 1097 1137 Size: 173 356 163 40 Fragments arranged by size: 356 173 163 110 65 64 46 45 40 32 27 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 564 1137 Size: 564 573 BsrBI CCG'CTC Cuts at: 0 819 1137 Size: 819 318 BsrFI r'CCGG_y Cuts at: 0 36 310 648 749 837 863 870 1002 Size: 36 274 338 101 88 26 7 132 Cuts at: 1002 1137 Size: 135 Fragments arranged by size: 338 274 135 132 101 88 36 26 7 BssSI C'ACGA_G Cuts at: 0 927 1137 Size: 927 210 BstYI r'GATC_y Cuts at: 0 714 1137 Size: 714 423 Bsu36I CC'TnA_GG Cuts at: 0 218 524 1137 Size: 218 306 613 Fragments arranged by size: 613 306 218 BtgI C'CryG_G Cuts at: 0 345 578 598 1137 Size: 345 233 20 539 Fragments arranged by size: 539 345 233 20 BtsI GCAGTG_nn' Cuts at: 0 38 1137 Size: 38 1099 EaeI y'GGCC_r Cuts at: 0 601 615 650 671 686 701 820 1137 Size: 601 14 35 21 15 15 119 317 Fragments arranged by size: 601 317 119 35 21 15 15 14 EagI C'GGCC_G Cuts at: 0 601 650 701 820 1137 Size: 601 49 51 119 317 Fragments arranged by size: 601 317 119 51 49 EarI CTCTTCn'nnn_ Cuts at: 0 353 1137 Size: 353 784 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 380 709 736 1137 Size: 380 329 27 401 Fragments arranged by size: 401 380 329 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 650 921 1006 1137 Size: 280 370 271 85 131 Fragments arranged by size: 370 280 271 131 85 EcoNI CCTnn'n_nnAGG Cuts at: 0 830 1137 Size: 830 307 EcoO109I rG'GnC_Cy Cuts at: 0 92 175 213 214 222 246 583 1078 Size: 92 83 38 1 8 24 337 495 Cuts at: 1078 1137 Size: 59 Fragments arranged by size: 495 337 92 83 59 38 24 8 1 EcoRI G'AATT_C Cuts at: 0 100 1137 Size: 100 1037 HaeII r_GCGC'y Cuts at: 0 598 877 1137 Size: 598 279 260 Fragments arranged by size: 598 279 260 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1062 1094 1137 Size: 1062 32 43 Fragments arranged by size: 1062 43 32 KasI G'GCGC_C Cuts at: 0 594 873 1137 Size: 594 279 264 Fragments arranged by size: 594 279 264 MspA1I CmG'CkG Cuts at: 0 111 347 416 580 600 670 1056 1084 Size: 111 236 69 164 20 70 386 28 Cuts at: 1084 1137 Size: 53 Fragments arranged by size: 386 236 164 111 70 69 53 28 20 NaeI GCC'GGC Cuts at: 0 38 312 650 839 865 872 1004 1137 Size: 38 274 338 189 26 7 132 133 Fragments arranged by size: 338 274 189 133 132 38 26 7 NarI GG'CG_CC Cuts at: 0 595 874 1137 Size: 595 279 263 Fragments arranged by size: 595 279 263 NgoMIV G'CCGG_C Cuts at: 0 36 310 648 837 863 870 1002 1137 Size: 36 274 338 189 26 7 132 135 Fragments arranged by size: 338 274 189 135 132 36 26 7 NotI GC'GGCC_GC Cuts at: 0 820 1137 Size: 820 317 PpuMI rG'GwC_Cy Cuts at: 0 92 583 1078 1137 Size: 92 491 495 59 Fragments arranged by size: 495 491 92 59 PspOMI G'GGCC_C Cuts at: 0 168 213 245 1137 Size: 168 45 32 892 Fragments arranged by size: 892 168 45 32 PvuI CG_AT'CG Cuts at: 0 738 1137 Size: 738 399 SacII CC_GC'GG Cuts at: 0 348 581 601 1137 Size: 348 233 20 536 Fragments arranged by size: 536 348 233 20 SanDI GG'GwC_CC Cuts at: 0 92 583 1078 1137 Size: 92 491 495 59 Fragments arranged by size: 495 491 92 59 SfiI GGCCn_nnn'nGGCC Cuts at: 0 819 1137 Size: 819 318 SfoI GGC'GCC Cuts at: 0 596 875 1137 Size: 596 279 262 Fragments arranged by size: 596 279 262 SgrAI Cr'CCGG_yG Cuts at: 0 310 1137 Size: 310 827 SmaI CCC'GGG Cuts at: 0 154 173 485 588 1137 Size: 154 19 312 103 549 Fragments arranged by size: 549 312 154 103 19 SmlI C'TyrA_G Cuts at: 0 490 1137 Size: 490 647 StyI C'CwwG_G Cuts at: 0 785 1137 Size: 785 352 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 27 902 934 1137 Size: 27 875 32 203 Fragments arranged by size: 875 203 32 27 XhoI C'TCGA_G Cuts at: 0 490 1137 Size: 490 647 XmaI C'CCGG_G Cuts at: 0 152 171 483 586 1137 Size: 152 19 312 103 551 Fragments arranged by size: 551 312 152 103 19 XmnI GAAnn'nnTTC Cuts at: 0 778 1137 Size: 778 359 ZraI GAC'GTC Cuts at: 0 327 1137 Size: 327 810 Enzymes that do cut: AarI AatII AcuI ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BglI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstYI Bsu36I BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KasI MspA1I NaeI NarI NgoMIV NotI PpuMI PspOMI PvuI SacII SanDI SfiI SfoI SgrAI SmaI SmlI StyI TstI XhoI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI