Restriction map for
pFN21AE4138
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AccI AclI AflII AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbvCI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaBI BsaI BsiWI BspLU11I
BsrDI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I CelII CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI HgaI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MamI MfeI MluI
MunI NarI NheI NotI NruI
PacI PaeR7I PflFI PflMI PleI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SalI SapI ScaI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI SspI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AatII 942 GACGTC GACGT^C
AccIII 809 TCCGGA T^CCGGA
AcsI 200 AAATTC [GA]^AATT[CT]
AcyI 942 GACGTC G[GA]^CG[CT]C
AflIII 860 ACGTGT A^C[GA][CT]GT
AgeI 977 ACCGGT A^CCGGT
AlwNI 634 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 200 AAATTC [GA]^AATT[CT]
BalI 641 TGGCCA TGG^CCA
BbrPI 1127 CACGTG CAC^GTG
BcgI 974 GCAACCGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1562 CTAG C^TAG
BglII 771 AGATCT A^GATCT
BsaAI 1127 CACGTG [CT]AC^GT[GA]
BsaHI 942 GACGTC G[GA]^CG[CT]C
BseAI 809 TCCGGA T^CCGGA
BsiEI 435 CGACCG CG[GA][CT]^CG
BsmBI 160 GAGACG CGTCTC(1/5)
Bsp1407I 153 TGTACA T^GTACA
BspDI 482 ATCGAT AT^CGAT
BspEI 809 TCCGGA T^CCGGA
BspHI 389 TCATGA T^CATGA
BspMI 1067 ACCTGC ACCTGC(4/8)
BsrBI 1498 GAGCGG CCGCTC(-3/-3)
BsrGI 153 TGTACA T^GTACA
BstAPI 654 GCACTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 116 TTCGAA TT^CGAA
BstXI 743 CCAAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 482 ATCGAT AT^CGAT
EaeI 641 TGGCCA [CT]^GGCC[GA]
EcoT22I 614 ATGCAT ATGCA^T
HaeII 929 AGCGCC [GA]GCGC^[CT]
Hin1I 942 GACGTC G[GA]^CG[CT]C
KspI 1192 CCGCGG CCGC^GG
MaeI 1562 CTAG C^TAG
MaeIII 735 GTCAC ^GT[ACGT]AC
MluNI 641 TGGCCA TGG^CCA
MroI 809 TCCGGA T^CCGGA
MscI 641 TGGCCA TGG^CCA
MslI 51 CATCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1119 GCCGGC GCC^GGC
NcoI 749 CCATGG C^CATGG
NdeI 527 CATATG CA^TATG
NgoMIV 1119 GCCGGC G^CCGGC
NsiI 614 ATGCAT ATGCA^T
NspI 720 GCATGT [GA]CATG^[CT]
NspV 116 TTCGAA TT^CGAA
PinAI 977 ACCGGT A^CCGGT
PmaCI 1127 CACGTG CAC^GTG
PmlI 1127 CACGTG CAC^GTG
RcaI 389 TCATGA T^CATGA
SacII 1192 CCGCGG CCGC^GG
SbfI 660 CCTGCAGG CCTGCA^GG
SfuI 116 TTCGAA TT^CGAA
SmlI 1018 CTCAAG C^T[CT][GA]AG
Sse8387I 660 CCTGCAGG CCTGCA^GG
SspBI 153 TGTACA T^GTACA
StuI 1111 AGGCCT AGG^CCT
Tsp45I 735 GTCAC ^GT[GC]AC
Tsp509I 201 AATT ^AATT
XcmI 1301 CCAGCCCGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 154 GTAC GT^AC
294 GTAC
AlwI 480 GGATC GGATC(4/5)
827 GGATC
ApaI 321 GGGCCC GGGCC^C
1395 GGGCCC
AvaI 835 CTCGGG C^[CT]CG[GA]G
1087 CCCGAG
BanI 1224 GGCACC G^G[CT][GA]CC
1546 GGCACC
BglI 677 GCCCAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1601 GCCTCTGCGGC
BsaWI 809 TCCGGA [AT]^CCGG[AT]
977 ACCGGT
BsgI 1520 CTGCAC GTGCAG(16/14)
631 GTGCAG
BsmAI 160 GAGAC GTCTC(1/5)
248 GAGAC
BsmI 302 GAATGC GAATGC(1/-1)
616 GCATTC
BsoBI 835 CTCGGG C^[CT]CG[GA]G
1087 CCCGAG
BsrFI 977 ACCGGT [GA]^CCGG[CT]
1119 GCCGGC
BstYI 771 AGATCT [GA]^GATC[CT]
827 GGATCT
Cfr10I 977 ACCGGT [GA]^CCGG[CT]
1119 GCCGGC
EcoT14I 749 CCATGG C^C[AT][AT]GG
1399 CCTTGG
HphI 1503 GGTGA GGTGA(8/7)
842 TCACC
MflI 771 AGATCT [GA]^GATC[CT]
827 GGATCT
MseI 457 TTAA T^TAA
914 TTAA
PvuII 634 CAGCTG CAG^CTG
1462 CAGCTG
RsaI 154 GTAC GT^AC
294 GTAC
StyI 749 CCATGG C^C[AT][AT]GG
1399 CCTTGG
Tru9I 457 TTAA T^TAA
914 TTAA
XhoII 771 AGATCT [GA]^GATC[CT]
827 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AluI AocI AspHI
AvaII BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI Eco81I EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MaeII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI TseI
TspRI TthHB8I