(Circular) (Six-base) MAPSORT of: pFN21AE5749.seq Check: 7013 from: 1 to: 6724 pFN21AE5749 6724 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3493 3493 Size: 6724 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4534 278 Size: 53 83 186 936 2998 2468 Fragments arranged by size: 2998 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3353 4067 1937 Size: 1416 714 4594 Fragments arranged by size: 4594 1416 714 Acc65I G'GTAC_C Cuts at: 4045 4045 Size: 6724 AclI AA'CG_TT Cuts at: 1563 4851 5224 1563 Size: 3288 373 3063 Fragments arranged by size: 3288 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3345 3557 4787 6171 6439 1632 Size: 1713 212 1230 1384 268 1917 Fragments arranged by size: 1917 1713 1384 1230 268 212 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6527 AflIII A'CryG_T Cuts at: 1203 3573 5623 6600 1203 Size: 2370 2050 977 1327 Fragments arranged by size: 2370 2050 1327 977 AgeI A'CCGG_T Cuts at: 5555 5555 Size: 6724 AhdI GACnn_n'nnGTC Cuts at: 2048 3321 5453 2048 Size: 1273 2132 3319 Fragments arranged by size: 3319 2132 1273 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6724 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2636 1184 Size: 1452 5272 AlwNI CAG_nnn'CTG Cuts at: 3368 3608 3833 4467 6039 3368 Size: 240 225 634 1572 4053 Fragments arranged by size: 4053 1572 634 240 225 ApaI G_GGCC'C Cuts at: 3711 3962 3711 Size: 251 6473 ApaLI G'TGCA_C Cuts at: 4781 5937 4781 Size: 1156 5568 ApoI r'AATT_y Cuts at: 1493 2157 4033 4259 4411 6520 1493 Size: 664 1876 226 152 2109 1697 Fragments arranged by size: 2109 1876 1697 664 226 152 AseI AT'TA_AT Cuts at: 160 1032 5278 160 Size: 872 4246 1606 Fragments arranged by size: 4246 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6724 AvaI C'yCGr_G Cuts at: 1870 1943 2241 2280 2435 2840 3054 3429 4049 Size: 73 298 39 155 405 214 375 620 Cuts at: 4049 1870 Size: 4545 Fragments arranged by size: 4545 620 405 375 298 214 155 73 39 BamHI G'GATC_C Cuts at: 3418 3541 4054 4421 3418 Size: 123 513 367 5721 Fragments arranged by size: 5721 513 367 123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2747 3050 3636 4045 5500 Size: 325 217 627 960 303 586 409 1455 Cuts at: 5500 618 Size: 1842 Fragments arranged by size: 1842 1455 960 627 586 409 325 303 217 BanII G_rGCy'C Cuts at: 1391 2538 3248 3393 3591 3711 3962 3981 4043 Size: 1147 710 145 198 120 251 19 62 Cuts at: 4043 1391 Size: 4072 Fragments arranged by size: 4072 1147 710 251 198 145 120 62 19 BbeI G_GCGC'C Cuts at: 3640 3640 Size: 6724 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2115 3675 3915 928 Size: 971 216 1560 240 3737 Fragments arranged by size: 3737 1560 971 240 216 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4915 4949 1903 Size: 34 2978 34 3678 Fragments arranged by size: 3678 2978 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4616 5826 4616 Size: 1210 5514 BclI T'GATC_A Cuts at: 1548 1548 Size: 6724 BfrBI ATG'CAT Cuts at: 2596 2596 Size: 6724 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5335 136 Size: 107 122 71 631 605 3663 1525 Fragments arranged by size: 3663 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6719 6719 Size: 6724 BlpI GC'TnA_GC Cuts at: 4141 4141 Size: 6724 Bme1580I G_kGCm'C Cuts at: 1790 2436 2901 3711 3962 4785 5941 1790 Size: 646 465 810 251 823 1156 2573 Fragments arranged by size: 2573 1156 823 810 646 465 251 BmgBI CAC'GTC Cuts at: 1318 3576 1318 Size: 2258 4466 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3468 4455 5413 Size: 416 53 83 399 24 2051 987 958 Cuts at: 5413 442 Size: 1753 Fragments arranged by size: 2051 1753 987 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6724 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3265 3265 Size: 6724 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2172 2381 3107 5384 1239 Size: 933 209 726 2277 2579 Fragments arranged by size: 2579 2277 933 726 209 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2891 4849 5714 6012 2891 Size: 1958 865 298 3603 Fragments arranged by size: 3603 1958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2801 3810 5387 882 Size: 617 1302 1009 1577 2219 Fragments arranged by size: 2219 1577 1302 1009 617 BsaAI yAC'GTr Cuts at: 493 1204 4515 493 Size: 711 3311 2702 Fragments arranged by size: 3311 2702 711 BsaBI GATnn'nnATC Cuts at: 6718 6718 Size: 6724 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3637 4531 4913 5548 Size: 53 83 186 936 2104 894 382 635 Cuts at: 5548 275 Size: 1451 Fragments arranged by size: 2104 1451 936 894 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5157 5555 5829 5976 6387 5157 Size: 398 274 147 411 5494 Fragments arranged by size: 5494 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6499 Fragments arranged by size: 6499 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2711 3035 3238 3241 3244 3401 1186 Size: 1525 324 203 3 3 157 4509 Fragments arranged by size: 4509 1525 324 203 157 3 3 BseYI C'CCAG_C Cuts at: 2584 3172 3338 3401 3622 5927 6423 2584 Size: 588 166 63 221 2305 496 2885 Fragments arranged by size: 2885 2305 588 496 221 166 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2797 1163 Size: 573 1061 5090 Fragments arranged by size: 5090 1061 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3455 4174 4935 5963 1540 Size: 275 186 1454 719 761 1028 2301 Fragments arranged by size: 2301 1454 1028 761 719 275 186 BsiHKAI G_wGCw'C Cuts at: 2538 3248 3981 4785 4870 5941 2538 Size: 710 733 804 85 1071 3321 Fragments arranged by size: 3321 1071 804 733 710 85 BsmI GAATG_Cn' Cuts at: 4235 4328 4235 Size: 93 6631 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2436 2538 2901 3248 3393 3591 3711 Size: 399 646 102 363 347 145 198 120 Cuts at: 3711 3962 3981 4043 4785 4870 5941 1391 Size: 251 19 62 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 646 399 363 347 251 198 145 120 102 85 62 19 BspEI T'CCGG_A Cuts at: 6387 6387 Size: 6724 BspHI T'CATG_A Cuts at: 4613 6343 4613 Size: 1730 4994 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2819 3114 3493 4079 844 Size: 1975 295 379 586 3489 Fragments arranged by size: 3489 1975 586 379 295 BsrBI CCG'CTC Cuts at: 1394 1823 3645 4611 1394 Size: 429 1822 966 3507 Fragments arranged by size: 3507 1822 966 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2644 3076 5219 5393 65 Size: 1183 1396 432 2143 174 1396 Fragments arranged by size: 2143 1396 1396 1183 432 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5368 5555 1700 Size: 110 3558 187 2869 Fragments arranged by size: 3558 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6724 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6724 BssSI C'ACGA_G Cuts at: 3852 4784 5796 3852 Size: 932 1012 4780 Fragments arranged by size: 4780 1012 932 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6724 BstBI TT'CG_AA Cuts at: 5528 5528 Size: 6724 BstEII G'GTnAC_C Cuts at: 1184 2419 2782 1184 Size: 1235 363 5126 Fragments arranged by size: 5126 1235 363 BstXI CCAn_nnnn'nTGG Cuts at: 2797 2908 2797 Size: 111 6613 BstYI r'GATC_y Cuts at: 1972 2440 3418 3541 4054 4421 4806 4823 6275 Size: 468 978 123 513 367 385 17 1452 Cuts at: 6275 6361 6373 6719 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 978 513 468 385 367 346 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3954 3954 Size: 6724 BtgI C'CryG_G Cuts at: 513 1065 2004 2424 3298 3304 3784 513 Size: 552 939 420 874 6 480 3453 Fragments arranged by size: 3453 939 874 552 480 420 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5985 Fragments arranged by size: 5985 683 56 BtsI GCAGTG_nn' Cuts at: 4240 5034 5054 4240 Size: 794 20 5910 Fragments arranged by size: 5910 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6724 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6689 DraI TTT'AAA Cuts at: 4028 4028 Size: 6724 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3677 3753 5731 809 Size: 507 2361 76 1978 1802 Fragments arranged by size: 2361 1978 1802 507 76 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2372 3301 3307 Size: 54 1425 50 275 18 542 929 6 Cuts at: 3307 3452 4171 5060 6689 8 Size: 145 719 889 1629 43 Fragments arranged by size: 1629 1425 929 889 719 542 275 145 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3452 4171 1537 Size: 275 1640 719 4090 Fragments arranged by size: 4090 1640 719 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2543 2799 3374 4654 1353 Size: 1190 256 575 1280 3423 Fragments arranged by size: 3423 1280 1190 575 256 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3973 5311 5685 5831 387 Size: 1064 138 2384 1338 374 146 1280 Fragments arranged by size: 2384 1338 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2536 3246 3979 2536 Size: 710 733 5281 Fragments arranged by size: 5281 733 710 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 2381 3107 3345 3557 4787 5384 Size: 393 540 209 726 238 212 1230 597 Cuts at: 5384 6171 6439 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1230 787 726 597 540 393 268 238 212 209 EcoNI CCTnn'n_nnAGG Cuts at: 3110 3110 Size: 6724 EcoO109I rG'GnC_Cy Cuts at: 2073 2716 3476 3958 3959 2073 Size: 643 760 482 1 4838 Fragments arranged by size: 4838 760 643 482 1 EcoRI G'AATT_C Cuts at: 4033 4411 4033 Size: 378 6346 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3932 3932 Size: 6724 FspI TGC'GCA Cuts at: 2612 5230 2612 Size: 2618 4106 HaeII r_GCGC'y Cuts at: 1678 3640 5871 1678 Size: 1962 2231 2531 Fragments arranged by size: 2531 2231 1962 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2034 2066 3048 3080 3230 Size: 32 135 32 462 32 982 32 150 Cuts at: 3230 3262 3307 3339 3356 3388 3833 3865 5365 Size: 32 45 32 17 32 445 32 1500 Cuts at: 5365 5397 5439 5471 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1500 982 462 445 150 135 45 42 32 32 32 32 32 32 32 32 32 32 17 HincII GTy'rAC Cuts at: 669 1657 1938 2550 4068 4314 669 Size: 988 281 612 1518 246 3079 Fragments arranged by size: 3079 1518 988 612 281 246 HindIII A'AGCT_T Cuts at: 748 3947 748 Size: 3199 3525 HpaI GTT'AAC Cuts at: 4314 4314 Size: 6724 KasI G'GCGC_C Cuts at: 3636 3636 Size: 6724 KpnI G_GTAC'C Cuts at: 4049 4049 Size: 6724 MfeI C'AATT_G Cuts at: 2733 4323 2733 Size: 1590 5134 MluI A'CGCG_T Cuts at: 6600 6600 Size: 6724 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 5838 6022 1305 Size: 786 3747 184 2007 Fragments arranged by size: 3747 2007 786 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4902 Fragments arranged by size: 4902 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2429 4682 5041 5200 518 Size: 648 1263 2253 359 159 2042 Fragments arranged by size: 2253 2042 1263 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2192 3207 4140 4817 5965 6210 1606 Size: 586 1015 933 677 1148 245 2120 Fragments arranged by size: 2120 1148 1015 933 677 586 245 NarI GG'CG_CC Cuts at: 3637 3637 Size: 6724 NcoI C'CATG_G Cuts at: 513 1065 2004 2424 513 Size: 552 939 420 4813 Fragments arranged by size: 4813 939 552 420 NdeI CA'TA_TG Cuts at: 387 387 Size: 6724 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6724 NotI GC'GGCC_GC Cuts at: 4171 4171 Size: 6724 NsiI A_TGCA'T Cuts at: 2598 2598 Size: 6724 NspI r_CATG'y Cuts at: 1132 1229 4082 5627 1132 Size: 97 2853 1545 2229 Fragments arranged by size: 2853 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2494 3015 1348 Size: 1146 521 5057 Fragments arranged by size: 5057 1146 521 PciI A'CATG_T Cuts at: 5623 5623 Size: 6724 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6724 PfoI T'CCnGG_A Cuts at: 2946 2946 Size: 6724 PmeI GTTT'AAAC Cuts at: 4028 4028 Size: 6724 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4793 4825 6306 6338 4793 Size: 32 1481 32 5179 Fragments arranged by size: 5179 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2716 3476 2716 Size: 760 5964 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6724 PsiI TTA'TAA Cuts at: 4294 4294 Size: 6724 PspOMI G'GGCC_C Cuts at: 3707 3958 3707 Size: 251 6473 PstI C_TGCA'G Cuts at: 830 3111 4076 830 Size: 2281 965 3478 Fragments arranged by size: 3478 2281 965 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6724 PvuII CAG'CTG Cuts at: 2192 2192 Size: 6724 SacI G_AGCT'C Cuts at: 2538 3248 3981 2538 Size: 710 733 5281 Fragments arranged by size: 5281 733 710 SalI G'TCGA_C Cuts at: 1936 4066 1936 Size: 2130 4594 SanDI GG'GwC_CC Cuts at: 3476 3476 Size: 6724 SapI GCTCTTCn'nnn_ Cuts at: 2543 2543 Size: 6724 SbfI CC_TGCA'GG Cuts at: 3111 4076 3111 Size: 965 5759 ScaI AGT'ACT Cuts at: 4972 6684 4972 Size: 1712 5012 SexAI A'CCwGG_T Cuts at: 2907 2907 Size: 6724 SfcI C'TryA_G Cuts at: 826 1045 3107 3354 3559 4072 5207 5888 6079 Size: 219 2062 247 205 513 1135 681 191 Cuts at: 6079 826 Size: 1471 Fragments arranged by size: 2062 1471 1135 681 513 247 219 205 191 SfoI GGC'GCC Cuts at: 3638 3638 Size: 6724 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6724 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6724 SmaI CCC'GGG Cuts at: 1872 2282 4051 1872 Size: 410 1769 4545 Fragments arranged by size: 4545 1769 410 SmlI C'TyrA_G Cuts at: 820 1017 1943 2241 2870 4828 5729 5991 820 Size: 197 926 298 629 1958 901 262 1553 Fragments arranged by size: 1958 1553 926 901 629 298 262 197 SnaBI TAC'GTA Cuts at: 493 4515 493 Size: 4022 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6724 SphI G_CATG'C Cuts at: 1132 4082 1132 Size: 2950 3774 SspI AAT'ATT Cuts at: 5 52 4648 5 Size: 47 4596 2081 Fragments arranged by size: 4596 2081 47 StuI AGG'CCT Cuts at: 1517 3693 1517 Size: 2176 4548 StyI C'CwwG_G Cuts at: 513 1065 2004 2424 3990 4163 513 Size: 552 939 420 1566 173 3074 Fragments arranged by size: 3074 1566 939 552 420 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2576 2789 4751 4768 4921 5106 5551 Size: 1255 996 213 1962 17 153 185 445 Cuts at: 5551 325 Size: 1498 Fragments arranged by size: 1962 1498 1255 996 445 213 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4970 6682 96 Size: 274 80 33 51 1443 2993 1712 138 Fragments arranged by size: 2993 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2731 2763 6684 6716 2731 Size: 32 3921 32 2739 Fragments arranged by size: 3921 2739 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2114 3370 3938 1316 Size: 798 1256 568 4102 Fragments arranged by size: 4102 1256 798 568 XbaI T'CTAG_A Cuts at: 4060 4060 Size: 6724 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6724 XhoI C'TCGA_G Cuts at: 1943 2241 1943 Size: 298 6426 XmaI C'CCGG_G Cuts at: 1870 2280 4049 1870 Size: 410 1769 4545 Fragments arranged by size: 4545 1769 410 XmnI GAAnn'nnTTC Cuts at: 4853 4853 Size: 6724 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4532 276 Size: 53 83 186 936 2998 2468 Fragments arranged by size: 2998 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SfiI SrfI SwaI