################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BciVI BclI BfaI BfrI BglI BglII BlnI BsaAI BsaBI BseAI BsmI BspDI BspEI BspLU11I BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoRV EcoT22I FbaI FseI FspI HaeII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 2026 GTCTAC GT^[AC][GT]AC AcyI 1033 GACGCC G[GA]^CG[CT]C Asp700I 1863 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1399 GAGCTC G[AT]GC[AT]^C BamHI 623 GGATCC G^GATCC BbsI 606 GAAGAC GAAGAC(2/6) BcgI 890 CGATCACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 2160 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2160 GCTCAGC GC^T[ACGT]AGC BpuAI 606 GAAGAC GAAGAC(2/6) BsaHI 1033 GACGCC G[GA]^CG[CT]C BsiHKAI 1399 GAGCTC G[AT]GC[AT]^C BsiWI 1506 CGTACG C^GTACG BsmBI 1031 GAGACG CGTCTC(1/5) Bsp1407I 965 TGTACA T^GTACA BsrGI 965 TGTACA T^GTACA BstEII 1611 GGTCACC G^GT[ACGT]ACC BstPI 1611 GGTCACC G^GT[ACGT]ACC BstYI 623 GGATCC [GA]^GATC[CT] CelII 2160 GCTCAGC GC^T[ACGT]AGC DraIII 1745 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1611 GGTCACC G^GT[ACGT]ACC EcoRI 1279 GAATTC G^AATTC Hin1I 1033 GACGCC G[GA]^CG[CT]C HincII 766 GTCAAC GT[CT]^[GA]AC HindII 766 GTCAAC GT[CT]^[GA]AC MflI 623 GGATCC [GA]^GATC[CT] MslI 1152 CATCGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 1143 ACATGC [GA]CATG^[CT] PaeR7I 442 CTCGAG C^TCGAG PvuII 2069 CAGCTG CAG^CTG SacI 1399 GAGCTC GAGCT^C SmlI 442 CTCGAG C^T[CT][GA]AG SspBI 965 TGTACA T^GTACA XhoI 442 CTCGAG C^TCGAG XhoII 623 GGATCC [GA]^GATC[CT] XmnI 1863 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AocI 304 CCTCAGG CC^T[ACGT]AGG 591 CCTCAGG ApaI 1272 GGGCCC GGGCC^C 1434 GGGCCC BalI 92 TGGCCA TGG^CCA 1079 TGGCCA BanI 147 GGCACC G^G[CT][GA]CC 361 GGCACC BbvCI 414 CCTCAGC CCTCAGC(-5/-2) 1578 CCTCAGC BsaWI 470 ACCGGA [AT]^CCGG[AT] 1361 ACCGGA BsgI 1708 CTGCAC GTGCAG(16/14) 1159 GTGCAG BsiEI 124 CGGCCG CG[GA][CT]^CG 1890 CGGCCG BspHI 1871 TCATGA T^CATGA 2087 TCATGA BspMI 875 ACCTGC ACCTGC(4/8) 1161 GCAGGT BsrFI 122 ACCGGC [GA]^CCGG[CT] 514 ACCGGC Bsu36I 304 CCTCAGG CC^T[ACGT]AGG 591 CCTCAGG Cfr10I 122 ACCGGC [GA]^CCGG[CT] 514 ACCGGC EagI 124 CGGCCG C^GGCCG 1890 CGGCCG EclXI 124 CGGCCG C^GGCCG 1890 CGGCCG Eco52I 124 CGGCCG C^GGCCG 1890 CGGCCG Eco81I 304 CCTCAGG CC^T[ACGT]AGG 591 CCTCAGG HindIII 208 AAGCTT A^AGCTT 646 AAGCTT MluNI 92 TGGCCA TGG^CCA 1079 TGGCCA MscI 92 TGGCCA TGG^CCA 1079 TGGCCA MseI 860 TTAA T^TAA 1490 TTAA MspA1I 1581 CAGCGG C[AC]G^C[GT]G 2069 CAGCTG PpuMI 228 GGGACCC [GA]G^G[AT]CC[CT] 344 AGGACCC PstI 898 CTGCAG CTGCA^G 1940 CTGCAG RcaI 1871 TCATGA T^CATGA 2087 TCATGA SapI 214 GAAGAGC GCTCTTC(1/4) 1695 GCTCTTC StuI 728 AGGCCT AGG^CCT 924 AGGCCT TfiI 704 GATTC G^A[AT]TC 958 GAATC Tru9I 860 TTAA T^TAA 1490 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 87 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI