(Circular) (Six-base) MAPSORT of: pFN21AE4210.seq Check: 3105 from: 1 to: 6895 pFN21AE4210 6895 bp With 182 enzymes: SgfI * March 28, 2012 13:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4705 278 Size: 53 83 186 936 3169 2468 Fragments arranged by size: 3169 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4032 4238 1937 Size: 2095 206 4594 Fragments arranged by size: 4594 2095 206 Acc65I G'GTAC_C Cuts at: 4216 4216 Size: 6895 AclI AA'CG_TT Cuts at: 1563 5022 5395 1563 Size: 3459 373 3063 Fragments arranged by size: 3459 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2630 4958 6342 6610 1632 Size: 464 534 2328 1384 268 1917 Fragments arranged by size: 2328 1917 1384 534 464 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6698 AflIII A'CryG_T Cuts at: 1203 5794 6771 1203 Size: 4591 977 1327 Fragments arranged by size: 4591 1327 977 AgeI A'CCGG_T Cuts at: 5726 5726 Size: 6895 AhdI GACnn_n'nnGTC Cuts at: 5624 5624 Size: 6895 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6895 AlwNI CAG_nnn'CTG Cuts at: 2095 2394 2821 4638 6210 2095 Size: 299 427 1817 1572 2780 Fragments arranged by size: 2780 1817 1572 427 299 ApaI G_GGCC'C Cuts at: 3281 3443 3281 Size: 162 6733 ApaLI G'TGCA_C Cuts at: 4952 6108 4952 Size: 1156 5739 ApoI r'AATT_y Cuts at: 1493 2786 3284 4148 4204 4430 4582 6691 1493 Size: 1293 498 864 56 226 152 2109 1697 Fragments arranged by size: 2109 1697 1293 864 498 226 152 56 AseI AT'TA_AT Cuts at: 160 1032 5449 160 Size: 872 4417 1606 Fragments arranged by size: 4417 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6895 AvaI C'yCGr_G Cuts at: 1870 1943 2447 3694 4045 4132 4220 1870 Size: 73 504 1247 351 87 88 4545 Fragments arranged by size: 4545 1247 504 351 88 87 73 BamHI G'GATC_C Cuts at: 2628 4225 4592 2628 Size: 1597 367 4931 Fragments arranged by size: 4931 1597 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2152 2366 4216 5671 618 Size: 325 217 627 365 214 1850 1455 1842 Fragments arranged by size: 1850 1842 1455 627 365 325 217 214 BanII G_rGCy'C Cuts at: 1391 2858 3281 3408 3443 3702 3725 4061 4214 Size: 1467 423 127 35 259 23 336 153 Cuts at: 4214 1391 Size: 4072 Fragments arranged by size: 4072 1467 423 336 259 153 127 35 23 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2618 928 Size: 971 719 5205 Fragments arranged by size: 5205 971 719 BbvCI CC'TCA_GC Cuts at: 2420 3584 2420 Size: 1164 5731 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2884 2918 5086 5120 1903 Size: 34 947 34 2168 34 3678 Fragments arranged by size: 3678 2168 947 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4787 5997 4787 Size: 1210 5685 BclI T'GATC_A Cuts at: 1548 1548 Size: 6895 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5506 136 Size: 107 122 71 631 605 3834 1525 Fragments arranged by size: 3834 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6890 6890 Size: 6895 BlpI GC'TnA_GC Cuts at: 4166 4312 4166 Size: 146 6749 Bme1580I G_kGCm'C Cuts at: 1790 2369 3234 3281 3443 3751 4956 6112 1790 Size: 579 865 47 162 308 1205 1156 2573 Fragments arranged by size: 2573 1205 1156 865 579 308 162 47 BmgBI CAC'GTC Cuts at: 1318 2938 1318 Size: 1620 5275 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2652 3249 3446 Size: 416 53 83 399 24 1235 597 197 Cuts at: 3446 4626 5584 442 Size: 1180 958 1753 Fragments arranged by size: 1753 1235 1180 958 597 416 399 197 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6895 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2843 3035 3380 3506 3624 3828 3904 Size: 974 630 192 345 126 118 204 76 Cuts at: 3904 5555 1239 Size: 1651 2579 Fragments arranged by size: 2579 1651 974 630 345 204 192 126 118 76 Bpu10I CC'TnA_GC Cuts at: 2420 3584 2420 Size: 1164 5731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5020 5885 6183 5020 Size: 865 298 5732 Fragments arranged by size: 5732 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3167 3182 3211 3524 3815 5558 882 Size: 617 1668 15 29 313 291 1743 2219 Fragments arranged by size: 2219 1743 1668 617 313 291 29 15 BsaAI yAC'GTr Cuts at: 493 1204 4686 493 Size: 711 3482 2702 Fragments arranged by size: 3482 2702 711 BsaBI GATnn'nnATC Cuts at: 6889 6889 Size: 6895 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3039 4702 5084 5719 Size: 53 83 186 936 1506 1663 382 635 Cuts at: 5719 275 Size: 1451 Fragments arranged by size: 1663 1506 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2475 3366 5328 5726 6000 6147 6558 2475 Size: 891 1962 398 274 147 411 2812 Fragments arranged by size: 2812 1962 891 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2140 2170 2984 3014 3440 Size: 30 165 30 737 30 814 30 426 Cuts at: 3440 3470 3800 3830 1178 Size: 30 330 30 4243 Fragments arranged by size: 4243 814 737 426 330 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2133 2414 2417 2840 2843 2864 2940 3461 Size: 947 281 3 423 3 21 76 521 Cuts at: 3461 3790 3904 3907 3983 1186 Size: 329 114 3 76 4098 Fragments arranged by size: 4098 947 521 423 329 281 114 76 76 21 3 3 3 BseYI C'CCAG_C Cuts at: 2270 2380 2857 2884 3042 3430 3836 4061 6098 Size: 110 477 27 158 388 406 225 2037 Cuts at: 6098 6594 2270 Size: 496 2571 Fragments arranged by size: 2571 2037 496 477 406 388 225 158 110 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3185 3698 1163 Size: 573 1449 513 4360 Fragments arranged by size: 4360 1449 573 513 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2132 3898 4345 5106 6134 1540 Size: 275 186 131 1766 447 761 1028 2301 Fragments arranged by size: 2301 1766 1028 761 447 275 186 131 BsiHKAI G_wGCw'C Cuts at: 3408 4956 5041 6112 3408 Size: 1548 85 1071 4191 Fragments arranged by size: 4191 1548 1071 85 BsiWI C'GTAC_G Cuts at: 3511 3511 Size: 6895 BsmI GAATG_Cn' Cuts at: 4406 4499 4406 Size: 93 6802 BsmBI CGTCTCn'nnnn_ Cuts at: 3030 3030 Size: 6895 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2369 2858 3234 3281 3408 3443 3702 Size: 399 579 489 376 47 127 35 259 Cuts at: 3702 3725 3751 4061 4214 4956 5041 6112 1391 Size: 23 26 310 153 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 579 489 399 376 310 259 153 127 85 47 35 26 23 BspEI T'CCGG_A Cuts at: 6558 6558 Size: 6895 BspHI T'CATG_A Cuts at: 3876 4092 4784 6514 3876 Size: 216 692 1730 4257 Fragments arranged by size: 4257 1730 692 216 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2889 3157 4250 844 Size: 2045 268 1093 3489 Fragments arranged by size: 3489 2045 1093 268 BsrBI CCG'CTC Cuts at: 1394 1823 2134 2626 3912 4782 1394 Size: 429 311 492 1286 870 3507 Fragments arranged by size: 3507 1286 870 492 429 311 BsrDI GCAATG_nn' Cuts at: 65 1248 5390 5564 65 Size: 1183 4142 174 1396 Fragments arranged by size: 4142 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2127 2519 5539 5726 1700 Size: 110 317 392 3020 187 2869 Fragments arranged by size: 3020 2869 392 317 187 110 BsrGI T'GTAC_A Cuts at: 96 2970 96 Size: 2874 4021 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6895 BssSI C'ACGA_G Cuts at: 4955 5967 4955 Size: 1012 5883 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6895 BstBI TT'CG_AA Cuts at: 5699 5699 Size: 6895 BstEII G'GTnAC_C Cuts at: 1184 3616 1184 Size: 2432 4463 BstYI r'GATC_y Cuts at: 1972 2628 4225 4592 4977 4994 6446 6532 6544 Size: 656 1597 367 385 17 1452 86 12 Cuts at: 6544 6890 1972 Size: 346 1977 Fragments arranged by size: 1977 1597 1452 656 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2310 2597 2310 Size: 287 6608 BtgI C'CryG_G Cuts at: 513 1065 2944 3472 3525 3534 3963 513 Size: 552 1879 528 53 9 429 3445 Fragments arranged by size: 3445 1879 552 528 429 53 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2361 487 Size: 683 56 1135 5021 Fragments arranged by size: 5021 1135 683 56 BtsI GCAGTG_nn' Cuts at: 2029 2703 2900 3213 3942 4411 5205 5225 2029 Size: 674 197 313 729 469 794 20 3699 Fragments arranged by size: 3699 794 729 674 469 313 197 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6895 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6860 DraI TTT'AAA Cuts at: 4199 4199 Size: 6895 DraIII CAC_nnn'GTG Cuts at: 3755 3755 Size: 6895 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5902 809 Size: 507 4586 1802 Fragments arranged by size: 4586 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2097 2129 Size: 54 1425 50 275 18 181 86 32 Cuts at: 2129 3084 3544 3895 4342 5231 6860 8 Size: 955 460 351 447 889 1629 43 Fragments arranged by size: 1629 1425 955 889 460 447 351 275 181 86 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 2129 3895 4342 1537 Size: 275 317 1766 447 4090 Fragments arranged by size: 4090 1766 447 317 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2214 2403 3707 4825 1353 Size: 861 189 1304 1118 3423 Fragments arranged by size: 3423 1304 1118 861 189 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5482 5856 6002 387 Size: 1064 138 3893 374 146 1280 Fragments arranged by size: 3893 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3406 3406 Size: 6895 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2213 2630 2843 3035 3380 3506 Size: 393 464 117 417 213 192 345 126 Cuts at: 3506 3624 3828 3904 4958 5555 6342 6610 1239 Size: 118 204 76 1054 597 787 268 1524 Fragments arranged by size: 1524 1054 787 597 464 417 393 345 268 213 204 192 126 118 117 76 EcoNI CCTnn'n_nnAGG Cuts at: 2476 2492 2825 2476 Size: 16 333 6546 Fragments arranged by size: 6546 333 16 EcoO109I rG'GnC_Cy Cuts at: 2234 2350 2469 3277 2234 Size: 116 119 808 5852 Fragments arranged by size: 5852 808 119 116 EcoRI G'AATT_C Cuts at: 3284 4204 4582 3284 Size: 920 378 5597 Fragments arranged by size: 5597 920 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2890 3646 2890 Size: 756 6139 FspI TGC'GCA Cuts at: 5401 5401 Size: 6895 HaeII r_GCGC'y Cuts at: 1678 6042 1678 Size: 4364 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2698 2730 3179 3211 3749 Size: 32 135 32 1126 32 449 32 538 Cuts at: 3749 3781 3800 3812 3832 3844 4087 4119 5536 Size: 32 19 12 20 12 243 32 1417 Cuts at: 5536 5568 5610 5642 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1417 1126 538 449 243 135 42 32 32 32 32 32 32 32 32 20 19 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2773 4239 4485 669 Size: 988 281 835 1466 246 3079 Fragments arranged by size: 3079 1466 988 835 281 246 HindIII A'AGCT_T Cuts at: 748 2213 2651 748 Size: 1465 438 4992 Fragments arranged by size: 4992 1465 438 HpaI GTT'AAC Cuts at: 4485 4485 Size: 6895 KpnI G_GTAC'C Cuts at: 4220 4220 Size: 6895 MfeI C'AATT_G Cuts at: 4494 4494 Size: 6895 MluI A'CGCG_T Cuts at: 6771 6771 Size: 6895 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2154 3321 6009 6193 1305 Size: 849 1167 2688 184 2007 Fragments arranged by size: 2688 2007 1167 849 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2099 3086 10 Size: 54 1425 343 267 987 3819 Fragments arranged by size: 3819 1425 987 343 267 54 MslI CAynn'nnrTG Cuts at: 518 1166 3161 4853 5212 5371 518 Size: 648 1995 1692 359 159 2042 Fragments arranged by size: 2042 1995 1692 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3588 4076 4311 4988 6136 6381 1606 Size: 1982 488 235 677 1148 245 2120 Fragments arranged by size: 2120 1982 1148 677 488 245 235 NcoI C'CATG_G Cuts at: 513 1065 3525 3534 3963 513 Size: 552 2460 9 429 3445 Fragments arranged by size: 3445 2460 552 429 9 NdeI CA'TA_TG Cuts at: 387 387 Size: 6895 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6895 NotI GC'GGCC_GC Cuts at: 4342 4342 Size: 6895 NspI r_CATG'y Cuts at: 1132 1229 3152 4253 5798 1132 Size: 97 1923 1101 1545 2229 Fragments arranged by size: 2229 1923 1545 1101 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6895 PciI A'CATG_T Cuts at: 5794 5794 Size: 6895 PflMI CCAn_nnn'nTGG Cuts at: 1108 2330 3526 3791 1108 Size: 1222 1196 265 4212 Fragments arranged by size: 4212 1222 1196 265 PfoI T'CCnGG_A Cuts at: 2982 3384 3483 2982 Size: 402 99 6394 Fragments arranged by size: 6394 402 99 PmeI GTTT'AAAC Cuts at: 4199 4199 Size: 6895 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4964 4996 6477 6509 4964 Size: 32 1481 32 5350 Fragments arranged by size: 5350 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2234 2350 2234 Size: 116 6779 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6895 PsiI TTA'TAA Cuts at: 4465 4465 Size: 6895 PspOMI G'GGCC_C Cuts at: 3277 3439 3277 Size: 162 6733 PspXI vC'TCGA_Gb Cuts at: 2447 2447 Size: 6895 PstI C_TGCA'G Cuts at: 830 2907 3949 4247 830 Size: 2077 1042 298 3478 Fragments arranged by size: 3478 2077 1042 298 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6895 PvuII CAG'CTG Cuts at: 4076 4076 Size: 6895 SacI G_AGCT'C Cuts at: 3408 3408 Size: 6895 SalI G'TCGA_C Cuts at: 1936 4237 1936 Size: 2301 4594 SanDI GG'GwC_CC Cuts at: 2234 2234 Size: 6895 SapI GCTCTTCn'nnn_ Cuts at: 2214 3707 2214 Size: 1493 5402 SbfI CC_TGCA'GG Cuts at: 4247 4247 Size: 6895 ScaI AGT'ACT Cuts at: 5143 6855 5143 Size: 1712 5183 SfcI C'TryA_G Cuts at: 826 1045 2903 3945 4033 4243 5378 6059 6250 Size: 219 1858 1042 88 210 1135 681 191 Cuts at: 6250 826 Size: 1471 Fragments arranged by size: 1858 1471 1135 1042 681 219 210 191 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6895 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6895 SmaI CCC'GGG Cuts at: 1872 4222 1872 Size: 2350 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2447 4999 5900 6162 820 Size: 197 926 504 2552 901 262 1553 Fragments arranged by size: 2552 1553 926 901 504 262 197 SnaBI TAC'GTA Cuts at: 493 4686 493 Size: 4193 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6895 SphI G_CATG'C Cuts at: 1132 4253 1132 Size: 3121 3774 SspI AAT'ATT Cuts at: 5 52 4819 5 Size: 47 4767 2081 Fragments arranged by size: 4767 2081 47 StuI AGG'CCT Cuts at: 1517 2735 2931 1517 Size: 1218 196 5481 Fragments arranged by size: 5481 1218 196 StyI C'CwwG_G Cuts at: 513 1065 2634 2730 3525 3534 3963 4334 513 Size: 552 1569 96 795 9 429 371 3074 Fragments arranged by size: 3074 1569 795 552 429 371 96 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3790 4922 4939 5092 5277 5722 325 Size: 1255 2210 1132 17 153 185 445 1498 Fragments arranged by size: 2210 1498 1255 1132 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2970 5141 6853 Size: 274 80 33 51 1443 993 2171 1712 Cuts at: 6853 96 Size: 138 Fragments arranged by size: 2171 1712 1443 993 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2008 2040 2140 2172 3226 3258 6855 6887 2008 Size: 32 100 32 1054 32 3597 32 2016 Fragments arranged by size: 3597 2016 1054 100 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6895 XbaI T'CTAG_A Cuts at: 4231 4231 Size: 6895 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2818 3433 3532 4178 2818 Size: 615 99 646 5535 Fragments arranged by size: 5535 646 615 99 XhoI C'TCGA_G Cuts at: 1943 2447 1943 Size: 504 6391 XmaI C'CCGG_G Cuts at: 1870 4220 1870 Size: 2350 4545 XmnI GAAnn'nnTTC Cuts at: 3872 5024 3872 Size: 1152 5743 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4703 276 Size: 53 83 186 936 3169 2468 Fragments arranged by size: 3169 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BstXI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI