(Linear) (Six-base) MAPSORT of: KIEE4210.seq Check: 4287 from: 1 to: 2190 KIEE4210.orf With 182 enzymes: SgfI * March 22, 2012 17:35 .. AccI GT'mk_AC Cuts at: 0 2027 2190 Size: 2027 163 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 625 2190 Size: 91 534 1565 Fragments arranged by size: 1565 534 91 AlwNI CAG_nnn'CTG Cuts at: 0 90 389 816 2190 Size: 90 299 427 1374 Fragments arranged by size: 1374 427 299 90 ApaI G_GGCC'C Cuts at: 0 1276 1438 2190 Size: 1276 162 752 Fragments arranged by size: 1276 752 162 ApoI r'AATT_y Cuts at: 0 781 1279 2143 2190 Size: 781 498 864 47 Fragments arranged by size: 864 781 498 47 AvaI C'yCGr_G Cuts at: 0 442 1689 2040 2127 2190 Size: 442 1247 351 87 63 Fragments arranged by size: 1247 442 351 87 63 BamHI G'GATC_C Cuts at: 0 623 2190 Size: 623 1567 BanI G'GyrC_C Cuts at: 0 147 361 2190 Size: 147 214 1829 Fragments arranged by size: 1829 214 147 BanII G_rGCy'C Cuts at: 0 853 1276 1403 1438 1697 1720 2056 2190 Size: 853 423 127 35 259 23 336 134 Fragments arranged by size: 853 423 336 259 134 127 35 23 BbsI GAAGACnn'nnnn_ Cuts at: 0 613 2190 Size: 613 1577 BbvCI CC'TCA_GC Cuts at: 0 415 1579 2190 Size: 415 1164 611 Fragments arranged by size: 1164 611 415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 879 913 2190 Size: 879 34 1277 Fragments arranged by size: 1277 879 34 BlpI GC'TnA_GC Cuts at: 0 2161 2190 Size: 2161 29 Bme1580I G_kGCm'C Cuts at: 0 364 1229 1276 1438 1746 2190 Size: 364 865 47 162 308 444 Fragments arranged by size: 865 444 364 308 162 47 BmgBI CAC'GTC Cuts at: 0 933 2190 Size: 933 1257 BmrI ACTGGGnnnn_n' Cuts at: 0 647 1244 1441 2190 Size: 647 597 197 749 Fragments arranged by size: 749 647 597 197 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 838 1030 1375 1501 1619 1823 1899 Size: 208 630 192 345 126 118 204 76 Cuts at: 1899 2190 Size: 291 Fragments arranged by size: 630 345 291 208 204 192 126 118 76 Bpu10I CC'TnA_GC Cuts at: 0 415 1579 2190 Size: 415 1164 611 Fragments arranged by size: 1164 611 415 BsaI GGTCTCn'nnnn_ Cuts at: 0 1162 1177 1206 1519 1810 2190 Size: 1162 15 29 313 291 380 Fragments arranged by size: 1162 380 313 291 29 15 BsaHI Gr'CG_yC Cuts at: 0 1034 2190 Size: 1034 1156 BsaWI w'CCGG_w Cuts at: 0 470 1361 2190 Size: 470 891 829 Fragments arranged by size: 891 829 470 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 135 165 979 1009 1435 1465 1795 1825 Size: 135 30 814 30 426 30 330 30 Cuts at: 1825 2190 Size: 365 Fragments arranged by size: 814 426 365 330 135 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 128 409 412 835 838 859 935 1456 Size: 128 281 3 423 3 21 76 521 Cuts at: 1456 1785 1899 1902 1978 2190 Size: 329 114 3 76 212 Fragments arranged by size: 521 423 329 281 212 128 114 76 76 21 3 3 3 BseYI C'CCAG_C Cuts at: 0 265 375 852 879 1037 1425 1831 2056 Size: 265 110 477 27 158 388 406 225 Cuts at: 2056 2190 Size: 134 Fragments arranged by size: 477 406 388 265 225 158 134 110 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1180 1693 2190 Size: 1180 513 497 Fragments arranged by size: 1180 513 497 BsiEI CG_ry'CG Cuts at: 0 127 1893 2190 Size: 127 1766 297 Fragments arranged by size: 1766 297 127 BsiHKAI G_wGCw'C Cuts at: 0 1403 2190 Size: 1403 787 BsiWI C'GTAC_G Cuts at: 0 1506 2190 Size: 1506 684 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1025 2190 Size: 1025 1165 Bsp1286I G_dGCh'C Cuts at: 0 364 853 1229 1276 1403 1438 1697 1720 Size: 364 489 376 47 127 35 259 23 Cuts at: 1720 1746 2056 2190 Size: 26 310 134 Fragments arranged by size: 489 376 364 310 259 134 127 47 35 26 23 BspHI T'CATG_A Cuts at: 0 1871 2087 2190 Size: 1871 216 103 Fragments arranged by size: 1871 216 103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 884 1152 2190 Size: 884 268 1038 Fragments arranged by size: 1038 884 268 BsrBI CCG'CTC Cuts at: 0 129 621 1907 2190 Size: 129 492 1286 283 Fragments arranged by size: 1286 492 283 129 BsrFI r'CCGG_y Cuts at: 0 122 514 2190 Size: 122 392 1676 Fragments arranged by size: 1676 392 122 BsrGI T'GTAC_A Cuts at: 0 965 2190 Size: 965 1225 BstEII G'GTnAC_C Cuts at: 0 1611 2190 Size: 1611 579 BstYI r'GATC_y Cuts at: 0 623 2190 Size: 623 1567 Bsu36I CC'TnA_GG Cuts at: 0 305 592 2190 Size: 305 287 1598 Fragments arranged by size: 1598 305 287 BtgI C'CryG_G Cuts at: 0 939 1467 1520 1529 1958 2190 Size: 939 528 53 9 429 232 Fragments arranged by size: 939 528 429 232 53 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 356 2190 Size: 356 1834 BtsI GCAGTG_nn' Cuts at: 0 24 698 895 1208 1937 2190 Size: 24 674 197 313 729 253 Fragments arranged by size: 729 674 313 253 197 24 DraIII CAC_nnn'GTG Cuts at: 0 1750 2190 Size: 1750 440 EaeI y'GGCC_r Cuts at: 0 6 92 124 1079 1539 1890 2190 Size: 6 86 32 955 460 351 300 Fragments arranged by size: 955 460 351 300 86 32 6 EagI C'GGCC_G Cuts at: 0 124 1890 2190 Size: 124 1766 300 Fragments arranged by size: 1766 300 124 EarI CTCTTCn'nnn_ Cuts at: 0 209 398 1702 2190 Size: 209 189 1304 488 Fragments arranged by size: 1304 488 209 189 EcoICRI GAG'CTC Cuts at: 0 1401 2190 Size: 1401 789 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 208 625 838 1030 1375 1501 1619 Size: 91 117 417 213 192 345 126 118 Cuts at: 1619 1823 1899 2190 Size: 204 76 291 Fragments arranged by size: 417 345 291 213 204 192 126 118 117 91 76 EcoNI CCTnn'n_nnAGG Cuts at: 0 471 487 820 2190 Size: 471 16 333 1370 Fragments arranged by size: 1370 471 333 16 EcoO109I rG'GnC_Cy Cuts at: 0 229 345 464 1272 2190 Size: 229 116 119 808 918 Fragments arranged by size: 918 808 229 119 116 EcoRI G'AATT_C Cuts at: 0 1279 2190 Size: 1279 911 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 885 1641 2190 Size: 885 756 549 Fragments arranged by size: 885 756 549 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 693 725 1174 1206 1744 1776 1795 1807 Size: 693 32 449 32 538 32 19 12 Cuts at: 1807 1827 1839 2082 2114 2190 Size: 20 12 243 32 76 Fragments arranged by size: 693 538 449 243 76 32 32 32 32 20 19 12 12 HincII GTy'rAC Cuts at: 0 768 2190 Size: 768 1422 HindIII A'AGCT_T Cuts at: 0 208 646 2190 Size: 208 438 1544 Fragments arranged by size: 1544 438 208 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 149 1316 2190 Size: 149 1167 874 Fragments arranged by size: 1167 874 149 MscI TGG'CCA Cuts at: 0 94 1081 2190 Size: 94 987 1109 Fragments arranged by size: 1109 987 94 MslI CAynn'nnrTG Cuts at: 0 1156 2190 Size: 1156 1034 MspA1I CmG'CkG Cuts at: 0 1583 2071 2190 Size: 1583 488 119 Fragments arranged by size: 1583 488 119 NcoI C'CATG_G Cuts at: 0 1520 1529 1958 2190 Size: 1520 9 429 232 Fragments arranged by size: 1520 429 232 9 NspI r_CATG'y Cuts at: 0 1147 2190 Size: 1147 1043 PflMI CCAn_nnn'nTGG Cuts at: 0 325 1521 1786 2190 Size: 325 1196 265 404 Fragments arranged by size: 1196 404 325 265 PfoI T'CCnGG_A Cuts at: 0 977 1379 1478 2190 Size: 977 402 99 712 Fragments arranged by size: 977 712 402 99 PpuMI rG'GwC_Cy Cuts at: 0 229 345 2190 Size: 229 116 1845 Fragments arranged by size: 1845 229 116 PspOMI G'GGCC_C Cuts at: 0 1272 1434 2190 Size: 1272 162 756 Fragments arranged by size: 1272 756 162 PspXI vC'TCGA_Gb Cuts at: 0 442 2190 Size: 442 1748 PstI C_TGCA'G Cuts at: 0 902 1944 2190 Size: 902 1042 246 Fragments arranged by size: 1042 902 246 PvuII CAG'CTG Cuts at: 0 2071 2190 Size: 2071 119 SacI G_AGCT'C Cuts at: 0 1403 2190 Size: 1403 787 SanDI GG'GwC_CC Cuts at: 0 229 2190 Size: 229 1961 SapI GCTCTTCn'nnn_ Cuts at: 0 209 1702 2190 Size: 209 1493 488 Fragments arranged by size: 1493 488 209 SfcI C'TryA_G Cuts at: 0 898 1940 2028 2190 Size: 898 1042 88 162 Fragments arranged by size: 1042 898 162 88 SmlI C'TyrA_G Cuts at: 0 442 2190 Size: 442 1748 StuI AGG'CCT Cuts at: 0 730 926 2190 Size: 730 196 1264 Fragments arranged by size: 1264 730 196 StyI C'CwwG_G Cuts at: 0 629 725 1520 1529 1958 2190 Size: 629 96 795 9 429 232 Fragments arranged by size: 795 629 429 232 96 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1785 2190 Size: 1785 405 TatI w'GTAC_w Cuts at: 0 965 2190 Size: 965 1225 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 3 35 135 167 1221 1253 2190 Size: 3 32 100 32 1054 32 937 Fragments arranged by size: 1054 937 100 32 32 32 3 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 813 1428 1527 2173 2190 Size: 813 615 99 646 17 Fragments arranged by size: 813 646 615 99 17 XhoI C'TCGA_G Cuts at: 0 442 2190 Size: 442 1748 XmnI GAAnn'nnTTC Cuts at: 0 1867 2190 Size: 1867 323 Enzymes that do cut: AccI AcuI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NspI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII SacI SanDI SapI SfcI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BglI BglII BmtI BplI BpuEI BsaAI BsaBI BsmI BspEI BsrDI BssHII BssSI BstAPI BstBI BstXI BstZ17I ClaI CspCI DraI DrdI EciI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmaI ZraI