Restriction map for
pFN21AE6688
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI HphI KpnI Ksp632I MaeIII
MamI MboII MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SalI SapI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
SwaI TaqI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AluI 610 AGCT AG^CT
AlwI 674 GATCC GGATC(4/5)
AscI 10 GGCGCGCC GG^CGCGCC
AviII 320 TGCGCA TGC^GCA
BcgI 48 CGAGGACTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 400 CTGGAG CTGGAG(16/14)
BseRI 148 GAGGAG GAGGAG(10/8)
BspMI 668 ACCTGC ACCTGC(4/8)
BsrI 563 CCAGT ACTGG(1/-1)
BssSI 531 CTCGTG CACGAG(-5/-1)
BstXI 563 CCAGTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoNI 588 CCTAGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 188 CCAAGG C^C[AT][AT]GG
FspI 320 TGCGCA TGC^GCA
HgaI 490 GACGC GACGC(5/10)
MaeII 212 ACGT A^CGT
NaeI 153 GCCGGC GCC^GGC
NgoMIV 153 GCCGGC G^CCGGC
NlaIII 201 CATG CATG^
PleI 52 GACTC GAGTC(4/5)
SbfI 463 CCTGCAGG CCTGCA^GG
Sse8387I 463 CCTGCAGG CCTGCA^GG
StyI 188 CCAAGG C^C[AT][AT]GG
TfiI 97 GATTC G^A[AT]TC
TspRI 564 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaII 185 GGTCC G^G[AT]CC
357 GGTCC
BbeI 445 GGCGCC GGCGC^C
614 GGCGCC
BfaI 22 CTAG C^TAG
589 CTAG
BsiEI 175 CGGCCG CG[GA][CT]^CG
416 CGGCCG
BsmFI 428 GGGAC GGGAC(10/14)
437 GGGAC
BsrFI 153 GCCGGC [GA]^CCGG[CT]
177 GCCGGT
Cfr10I 153 GCCGGC [GA]^CCGG[CT]
177 GCCGGT
DpnII 309 GATC ^GATC
674 GATC
EagI 175 CGGCCG C^GGCCG
416 CGGCCG
EclXI 175 CGGCCG C^GGCCG
416 CGGCCG
Eco52I 175 CGGCCG C^GGCCG
416 CGGCCG
HinfI 52 GACTC G^A[ACGT]TC
97 GATTC
KasI 445 GGCGCC G^GCGCC
614 GGCGCC
KspI 381 CCGCGG CCGC^GG
658 CCGCGG
MaeI 22 CTAG C^TAG
589 CTAG
MboI 309 GATC ^GATC
674 GATC
NarI 445 GGCGCC GG^CGCC
614 GGCGCC
NdeII 309 GATC ^GATC
674 GATC
PstI 129 CTGCAG CTGCA^G
464 CTGCAG
SacII 381 CCGCGG CCGC^GG
658 CCGCGG
Sau3AI 309 GATC ^GATC
674 GATC
SfaNI 230 GCATC GCATC(5/9)
338 GCATC
SfcI 129 CTGCAG C^T[GA][CT]AG
464 CTGCAG
SfoI 445 GGCGCC GGC^GCC
614 GGCGCC
SmaI 88 CCCGGG CCC^GGG
682 CCCGGG
XmaCI 88 CCCGGG C^CCGGG
682 CCCGGG
XmaI 88 CCCGGG C^CCGGG
682 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AccII AciI AcyI ApaI AvaI
BanI BanII BbvI BcnI BglI
BmyI BsaHI BsaJI BsiYI BslI
BsoBI Bsp1286I BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
ItaI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIV Sau96I
ScrFI StuI TseI