(Circular) (Six-base) MAPSORT of: pFN21AE6688.seq Check: 4696 from: 1 to: 5410 pFN21AE6688 5410 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3220 278 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2753 1937 Size: 816 4594 Acc65I G'GTAC_C Cuts at: 2731 2731 Size: 5410 AclI AA'CG_TT Cuts at: 1563 3537 3910 1563 Size: 1974 373 3063 Fragments arranged by size: 3063 1974 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3473 4857 5125 1632 Size: 1841 1384 268 1917 Fragments arranged by size: 1917 1841 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5213 AflIII A'CryG_T Cuts at: 1203 4309 5286 1203 Size: 3106 977 1327 Fragments arranged by size: 3106 1327 977 AgeI A'CCGG_T Cuts at: 4241 4241 Size: 5410 AhdI GACnn_n'nnGTC Cuts at: 4139 4139 Size: 5410 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5410 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5410 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2114 2146 2114 Size: 32 5378 AlwNI CAG_nnn'CTG Cuts at: 3153 4725 3153 Size: 1572 3838 ApaI G_GGCC'C Cuts at: 2081 2394 2478 2081 Size: 313 84 5013 Fragments arranged by size: 5013 313 84 ApaLI G'TGCA_C Cuts at: 3467 4623 3467 Size: 1156 4254 ApoI r'AATT_y Cuts at: 1493 2719 2945 3097 5206 1493 Size: 1226 226 152 2109 1697 Fragments arranged by size: 2109 1697 1226 226 152 AscI GG'CGCG_CC Cuts at: 2016 2016 Size: 5410 AseI AT'TA_AT Cuts at: 160 1032 3964 160 Size: 872 2932 1606 Fragments arranged by size: 2932 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2093 2687 2735 1870 Size: 73 144 6 594 48 4545 Fragments arranged by size: 4545 594 144 73 48 6 BamHI G'GATC_C Cuts at: 2740 3107 2740 Size: 367 5043 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2450 2512 2619 2731 4186 Size: 325 217 627 663 62 107 112 1455 Cuts at: 4186 618 Size: 1842 Fragments arranged by size: 1842 1455 663 627 325 217 112 107 62 BanII G_rGCy'C Cuts at: 1391 2081 2094 2394 2478 2729 1391 Size: 690 13 300 84 251 4072 Fragments arranged by size: 4072 690 300 251 84 13 BbeI G_GCGC'C Cuts at: 2454 2623 2454 Size: 169 5241 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2042 2076 3601 3635 1903 Size: 34 50 34 21 34 1525 34 3678 Fragments arranged by size: 3678 1525 50 34 34 34 34 21 BciVI GTATCCnnnnn_n' Cuts at: 3302 4512 3302 Size: 1210 4200 BclI T'GATC_A Cuts at: 1548 1548 Size: 5410 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2389 2473 2659 Size: 107 122 71 631 605 717 84 186 Cuts at: 2659 4021 136 Size: 1362 1525 Fragments arranged by size: 1525 1362 717 631 605 186 122 107 84 71 BglII A'GATC_T Cuts at: 5405 5405 Size: 5410 BlpI GC'TnA_GC Cuts at: 2827 2827 Size: 5410 Bme1580I G_kGCm'C Cuts at: 1790 2081 2394 2478 2543 3471 4627 1790 Size: 291 313 84 65 928 1156 2573 Fragments arranged by size: 2573 1156 928 313 291 84 65 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5410 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2562 3141 4099 Size: 416 53 83 399 24 1145 579 958 Cuts at: 4099 442 Size: 1753 Fragments arranged by size: 1753 1145 958 579 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5410 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2426 4070 1239 Size: 1187 1644 2579 Fragments arranged by size: 2579 1644 1187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3535 4400 4698 3535 Size: 865 298 4247 Fragments arranged by size: 4247 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4073 882 Size: 617 2574 2219 Fragments arranged by size: 2574 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3201 493 Size: 711 1997 2702 Fragments arranged by size: 2702 1997 711 BsaBI GATnn'nnATC Cuts at: 5404 5404 Size: 5410 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2451 2496 2620 3217 Size: 53 83 186 936 918 45 124 597 Cuts at: 3217 3599 4234 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 918 635 597 382 186 124 83 53 45 BsaWI w'CCGG_w Cuts at: 3843 4241 4515 4662 5073 3843 Size: 398 274 147 411 4180 Fragments arranged by size: 4180 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5185 Fragments arranged by size: 5185 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 1186 Size: 982 4428 BseYI C'CCAG_C Cuts at: 2113 2477 4613 5109 2113 Size: 364 2136 496 2414 Fragments arranged by size: 2414 2136 496 364 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4837 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2183 2424 2860 3621 4649 1540 Size: 275 186 182 241 436 761 1028 2301 Fragments arranged by size: 2301 1028 761 436 275 241 186 182 BsiHKAI G_wGCw'C Cuts at: 3471 3556 4627 3471 Size: 85 1071 4254 Fragments arranged by size: 4254 1071 85 BsmI GAATG_Cn' Cuts at: 2921 3014 2921 Size: 93 5317 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2081 2094 2394 2478 2543 2729 3471 Size: 399 291 13 300 84 65 186 742 Cuts at: 3471 3556 4627 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 300 291 186 85 84 65 13 BspEI T'CCGG_A Cuts at: 5073 5073 Size: 5410 BspHI T'CATG_A Cuts at: 3299 5029 3299 Size: 1730 3680 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2682 2765 844 Size: 1838 83 3489 Fragments arranged by size: 3489 1838 83 BsrBI CCG'CTC Cuts at: 1394 1823 3297 1394 Size: 429 1474 3507 Fragments arranged by size: 3507 1474 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3905 4079 65 Size: 1183 2657 174 1396 Fragments arranged by size: 2657 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2158 2182 4054 4241 1700 Size: 110 348 24 1872 187 2869 Fragments arranged by size: 2869 1872 348 187 110 24 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5410 BssHII G'CGCG_C Cuts at: 1925 2016 2173 2175 2368 2426 2486 1925 Size: 91 157 2 193 58 60 4849 Fragments arranged by size: 4849 193 157 91 60 58 2 BssSI C'ACGA_G Cuts at: 2536 3470 4482 2536 Size: 934 1012 3464 Fragments arranged by size: 3464 1012 934 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5410 BstBI TT'CG_AA Cuts at: 4214 4214 Size: 5410 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5410 BstXI CCAn_nnnn'nTGG Cuts at: 2575 2575 Size: 5410 BstYI r'GATC_y Cuts at: 1972 2740 3107 3492 3509 4961 5047 5059 5405 Size: 768 367 385 17 1452 86 12 346 Cuts at: 5405 1972 Size: 1977 Fragments arranged by size: 1977 1452 768 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2386 2413 2663 513 Size: 552 1321 27 250 3260 Fragments arranged by size: 3260 1321 552 250 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2329 487 Size: 683 56 1103 3568 Fragments arranged by size: 3568 1103 683 56 BtsI GCAGTG_nn' Cuts at: 2926 3720 3740 2926 Size: 794 20 4596 Fragments arranged by size: 4596 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5410 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5375 DraI TTT'AAA Cuts at: 2714 2714 Size: 5410 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4417 809 Size: 507 3101 1802 Fragments arranged by size: 3101 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2180 2208 2421 Size: 54 1425 50 275 18 350 28 213 Cuts at: 2421 2577 2660 2857 3746 5375 8 Size: 156 83 197 889 1629 43 Fragments arranged by size: 1629 1425 889 350 275 213 197 156 83 54 50 43 28 18 EagI C'GGCC_G Cuts at: 1537 1812 2180 2421 2857 1537 Size: 275 368 241 436 4090 Fragments arranged by size: 4090 436 368 275 241 EarI CTCTTCn'nnn_ Cuts at: 1353 3340 1353 Size: 1987 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2165 2671 3997 4371 4517 387 Size: 1064 138 576 506 1326 374 146 1280 Fragments arranged by size: 1326 1280 1064 576 506 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2426 3473 4070 4857 5125 1239 Size: 393 794 1047 597 787 268 1524 Fragments arranged by size: 1524 1047 794 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2597 2597 Size: 5410 EcoO109I rG'GnC_Cy Cuts at: 2077 2078 2391 2474 2077 Size: 1 313 83 5013 Fragments arranged by size: 5013 313 83 1 EcoRI G'AATT_C Cuts at: 2719 3097 2719 Size: 378 5032 FspI TGC'GCA Cuts at: 2327 3916 2327 Size: 1589 3821 HaeII r_GCGC'y Cuts at: 1678 2130 2279 2454 2623 4557 1678 Size: 452 149 175 169 1934 2531 Fragments arranged by size: 2531 1934 452 175 169 149 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2016 2048 2683 2715 4051 Size: 32 135 32 444 32 635 32 1336 Cuts at: 4051 4083 4125 4157 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1336 635 444 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2754 3000 669 Size: 988 281 816 246 3079 Fragments arranged by size: 3079 988 816 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5410 HpaI GTT'AAC Cuts at: 3000 3000 Size: 5410 KasI G'GCGC_C Cuts at: 2450 2619 2450 Size: 169 5241 KpnI G_GTAC'C Cuts at: 2735 2735 Size: 5410 MfeI C'AATT_G Cuts at: 3009 3009 Size: 5410 MluI A'CGCG_T Cuts at: 5286 5286 Size: 5410 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4524 4708 1305 Size: 3219 184 2007 Fragments arranged by size: 3219 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3588 Fragments arranged by size: 3588 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3368 3727 3886 518 Size: 648 2202 359 159 2042 Fragments arranged by size: 2202 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2388 2529 2625 2665 2826 3503 4651 4896 Size: 782 141 96 40 161 677 1148 245 Cuts at: 4896 1606 Size: 2120 Fragments arranged by size: 2120 1148 782 677 245 161 141 96 40 NaeI GCC'GGC Cuts at: 2160 2160 Size: 5410 NarI GG'CG_CC Cuts at: 2451 2620 2451 Size: 169 5241 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4858 NdeI CA'TA_TG Cuts at: 387 387 Size: 5410 NgoMIV G'CCGG_C Cuts at: 2158 2158 Size: 5410 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5410 NotI GC'GGCC_GC Cuts at: 2857 2857 Size: 5410 NspI r_CATG'y Cuts at: 1132 1229 2768 4313 1132 Size: 97 1539 1545 2229 Fragments arranged by size: 2229 1545 1539 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5410 PciI A'CATG_T Cuts at: 4309 4309 Size: 5410 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5410 PfoI T'CCnGG_A Cuts at: 2238 2238 Size: 5410 PmeI GTTT'AAAC Cuts at: 2714 2714 Size: 5410 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3479 3511 4992 5024 3479 Size: 32 1481 32 3865 Fragments arranged by size: 3865 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5410 PsiI TTA'TAA Cuts at: 2980 2980 Size: 5410 PspOMI G'GGCC_C Cuts at: 2077 2390 2474 2077 Size: 313 84 5013 Fragments arranged by size: 5013 313 84 PstI C_TGCA'G Cuts at: 830 2138 2473 2762 830 Size: 1308 335 289 3478 Fragments arranged by size: 3478 1308 335 289 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5410 SacII CC_GC'GG Cuts at: 2389 2666 2389 Size: 277 5133 SalI G'TCGA_C Cuts at: 1936 2752 1936 Size: 816 4594 SbfI CC_TGCA'GG Cuts at: 2473 2762 2473 Size: 289 5121 ScaI AGT'ACT Cuts at: 3658 5370 3658 Size: 1712 3698 SfcI C'TryA_G Cuts at: 826 1045 2134 2469 2758 3893 4574 4765 826 Size: 219 1089 335 289 1135 681 191 1471 Fragments arranged by size: 1471 1135 1089 681 335 289 219 191 SfoI GGC'GCC Cuts at: 2452 2621 2452 Size: 169 5241 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5410 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5410 SmaI CCC'GGG Cuts at: 1872 2095 2689 2737 1872 Size: 223 594 48 4545 Fragments arranged by size: 4545 594 223 48 SmlI C'TyrA_G Cuts at: 820 1017 1943 3514 4415 4677 820 Size: 197 926 1571 901 262 1553 Fragments arranged by size: 1571 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3201 493 Size: 2708 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5410 SphI G_CATG'C Cuts at: 1132 2768 1132 Size: 1636 3774 SspI AAT'ATT Cuts at: 5 52 3334 5 Size: 47 3282 2081 Fragments arranged by size: 3282 2081 47 StuI AGG'CCT Cuts at: 1517 2031 2255 2603 1517 Size: 514 224 348 4324 Fragments arranged by size: 4324 514 348 224 StyI C'CwwG_G Cuts at: 513 1065 2193 2849 513 Size: 552 1128 656 3074 Fragments arranged by size: 3074 1128 656 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3437 3454 3607 3792 4237 325 Size: 1255 1857 17 153 185 445 1498 Fragments arranged by size: 1857 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3656 5368 96 Size: 274 80 33 51 1443 1679 1712 138 Fragments arranged by size: 1712 1679 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5370 5402 5370 Size: 32 5378 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5410 XbaI T'CTAG_A Cuts at: 2746 2746 Size: 5410 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5410 XmaI C'CCGG_G Cuts at: 1870 2093 2687 2735 1870 Size: 223 594 48 4545 Fragments arranged by size: 4545 594 223 48 XmnI GAAnn'nnTTC Cuts at: 3539 3539 Size: 5410 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3218 276 Size: 53 83 186 936 1684 2468 Fragments arranged by size: 2468 1684 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI