(Linear) (Six-base) MAPSORT of: KIEE6688.seq Check: 917 from: 1 to: 705 KIEE6688.orf With 182 enzymes: SgfI * March 16, 2015 11:35 .. AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 109 141 705 Size: 109 32 564 Fragments arranged by size: 564 109 32 ApaI G_GGCC'C Cuts at: 0 76 389 473 705 Size: 76 313 84 232 Fragments arranged by size: 313 232 84 76 AscI GG'CGCG_CC Cuts at: 0 11 705 Size: 11 694 AvaI C'yCGr_G Cuts at: 0 82 88 682 705 Size: 82 6 594 23 Fragments arranged by size: 594 82 23 6 BanI G'GyrC_C Cuts at: 0 445 507 614 705 Size: 445 62 107 91 Fragments arranged by size: 445 107 91 62 BanII G_rGCy'C Cuts at: 0 76 89 389 473 705 Size: 76 13 300 84 232 Fragments arranged by size: 300 232 84 76 13 BbeI G_GCGC'C Cuts at: 0 449 618 705 Size: 449 169 87 Fragments arranged by size: 449 169 87 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 37 71 705 Size: 37 34 634 Fragments arranged by size: 634 37 34 BglI GCCn_nnn'nGGC Cuts at: 0 384 468 654 705 Size: 384 84 186 51 Fragments arranged by size: 384 186 84 51 Bme1580I G_kGCm'C Cuts at: 0 76 389 473 538 705 Size: 76 313 84 65 167 Fragments arranged by size: 313 167 84 76 65 BmrI ACTGGGnnnn_n' Cuts at: 0 557 705 Size: 557 148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 421 705 Size: 421 284 BsaHI Gr'CG_yC Cuts at: 0 446 491 615 705 Size: 446 45 124 90 Fragments arranged by size: 446 124 90 45 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 163 705 Size: 163 542 BseYI C'CCAG_C Cuts at: 0 108 472 705 Size: 108 364 233 Fragments arranged by size: 364 233 108 BsiEI CG_ry'CG Cuts at: 0 178 419 705 Size: 178 241 286 Fragments arranged by size: 286 241 178 Bsp1286I G_dGCh'C Cuts at: 0 76 89 389 473 538 705 Size: 76 13 300 84 65 167 Fragments arranged by size: 300 167 84 76 65 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 677 705 Size: 677 28 BsrFI r'CCGG_y Cuts at: 0 153 177 705 Size: 153 24 528 Fragments arranged by size: 528 153 24 BssHII G'CGCG_C Cuts at: 0 11 168 170 363 421 481 705 Size: 11 157 2 193 58 60 224 Fragments arranged by size: 224 193 157 60 58 11 2 BssSI C'ACGA_G Cuts at: 0 531 705 Size: 531 174 BstXI CCAn_nnnn'nTGG Cuts at: 0 570 705 Size: 570 135 BtgI C'CryG_G Cuts at: 0 381 408 658 705 Size: 381 27 250 47 Fragments arranged by size: 381 250 47 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 324 705 Size: 324 381 EaeI y'GGCC_r Cuts at: 0 175 203 416 572 655 705 Size: 175 28 213 156 83 50 Fragments arranged by size: 213 175 156 83 50 28 EagI C'GGCC_G Cuts at: 0 175 416 705 Size: 175 241 289 Fragments arranged by size: 289 241 175 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 160 666 705 Size: 160 506 39 Fragments arranged by size: 506 160 39 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 421 705 Size: 421 284 EcoNI CCTnn'n_nnAGG Cuts at: 0 592 705 Size: 592 113 EcoO109I rG'GnC_Cy Cuts at: 0 72 73 386 469 705 Size: 72 1 313 83 236 Fragments arranged by size: 313 236 83 72 1 FspI TGC'GCA Cuts at: 0 322 705 Size: 322 383 HaeII r_GCGC'y Cuts at: 0 125 274 449 618 705 Size: 125 149 175 169 87 Fragments arranged by size: 175 169 149 125 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 11 43 678 705 Size: 11 32 635 27 Fragments arranged by size: 635 32 27 11 KasI G'GCGC_C Cuts at: 0 445 614 705 Size: 445 169 91 Fragments arranged by size: 445 169 91 MspA1I CmG'CkG Cuts at: 0 383 524 620 660 705 Size: 383 141 96 40 45 Fragments arranged by size: 383 141 96 45 40 NaeI GCC'GGC Cuts at: 0 155 705 Size: 155 550 NarI GG'CG_CC Cuts at: 0 446 615 705 Size: 446 169 90 Fragments arranged by size: 446 169 90 NgoMIV G'CCGG_C Cuts at: 0 153 705 Size: 153 552 PfoI T'CCnGG_A Cuts at: 0 233 705 Size: 233 472 PspOMI G'GGCC_C Cuts at: 0 72 385 469 705 Size: 72 313 84 236 Fragments arranged by size: 313 236 84 72 PstI C_TGCA'G Cuts at: 0 133 468 705 Size: 133 335 237 Fragments arranged by size: 335 237 133 SacII CC_GC'GG Cuts at: 0 384 661 705 Size: 384 277 44 Fragments arranged by size: 384 277 44 SbfI CC_TGCA'GG Cuts at: 0 468 705 Size: 468 237 SfcI C'TryA_G Cuts at: 0 129 464 705 Size: 129 335 241 Fragments arranged by size: 335 241 129 SfoI GGC'GCC Cuts at: 0 447 616 705 Size: 447 169 89 Fragments arranged by size: 447 169 89 SmaI CCC'GGG Cuts at: 0 90 684 705 Size: 90 594 21 Fragments arranged by size: 594 90 21 StuI AGG'CCT Cuts at: 0 26 250 598 705 Size: 26 224 348 107 Fragments arranged by size: 348 224 107 26 StyI C'CwwG_G Cuts at: 0 188 705 Size: 188 517 XmaI C'CCGG_G Cuts at: 0 88 682 705 Size: 88 594 23 Fragments arranged by size: 594 88 23 Enzymes that do cut: AloI ApaI AscI AvaI BanI BanII BbeI BcgI BglI Bme1580I BmrI BpmI BsaHI BseRI BseYI BsiEI Bsp1286I BspMI BsrFI BssHII BssSI BstXI BtgI BtgZI EaeI EagI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MspA1I NaeI NarI NgoMIV PfoI PspOMI PstI SacII SbfI SfcI SfoI SmaI StuI StyI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI