################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccII AccIII AflII AflIII AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvrII BbrPI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BstBI BstEII BstPI BstUI ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MamI MluI MroI MvnI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacI SacII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TfiI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 717 GTATAC GT^[AC][GT]AC AclI 324 AACGTT AA^CGTT AcsI 831 AAATTT [GA]^AATT[CT] AcyI 1414 GGCGCC G[GA]^CG[CT]C AfaI 647 GTAC GT^AC AgeI 214 ACCGGT A^CCGGT AocI 766 CCTCAGG CC^T[ACGT]AGG ApoI 831 AAATTT [GA]^AATT[CT] Asp700I 28 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 1481 TGCGCA TGC^GCA BamHI 1840 GGATCC G^GATCC BbeI 1414 GGCGCC GGCGC^C BbsI 339 GTCTTC GAAGAC(2/6) BbvCI 202 GCTGAGG CCTCAGC(-5/-2) BfaI 1520 CTAG C^TAG BlpI 388 GCTCAGC GC^T[ACGT]AGC Bpu1102I 388 GCTCAGC GC^T[ACGT]AGC BpuAI 339 GTCTTC GAAGAC(2/6) BsaHI 1414 GGCGCC G[GA]^CG[CT]C BsgI 1330 GTGCAG GTGCAG(16/14) BsiEI 1526 CGGTCG CG[GA][CT]^CG BsmI 28 GAATGC GAATGC(1/-1) BsrBI 1497 GAGCGG CCGCTC(-3/-3) BsrDI 1234 GCAATG GCAATG(2/0) BssSI 821 CACGAG CACGAG(-5/-1) Bst1107I 717 GTATAC GTA^TAC BstZ17I 717 GTATAC GTA^TAC Bsu36I 766 CCTCAGG CC^T[ACGT]AGG CelII 388 GCTCAGC GC^T[ACGT]AGC DraIII 517 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1000 GACCTTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 766 CCTCAGG CC^T[ACGT]AGG EcoNI 736 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FspI 1481 TGCGCA TGC^GCA HgaI 337 GCGTC GACGC(5/10) Hin1I 1414 GGCGCC G[GA]^CG[CT]C KasI 1414 GGCGCC G^GCGCC MaeI 1520 CTAG C^TAG MfeI 628 CAATTG C^AATTG MunI 628 CAATTG C^AATTG NaeI 1878 GCCGGC GCC^GGC NarI 1414 GGCGCC GG^CGCC NdeI 527 CATATG CA^TATG NgoMIV 1878 GCCGGC G^CCGGC PinAI 214 ACCGGT A^CCGGT Psp1406I 324 AACGTT AA^CGTT RsaI 647 GTAC GT^AC SapI 1869 GAAGAGC GCTCTTC(1/4) ScaI 646 AGTACT AGT^ACT SexAI 1256 ACCTGGT A^CC[AT]GGT SfoI 1414 GGCGCC GGC^GCC SmlI 1003 CTTGAG C^T[CT][GA]AG TaqI 1610 TCGA T^CGA TthHB8I 1610 TCGA T^CGA XmnI 28 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### BalI 1644 TGGCCA TGG^CCA 1942 TGGCCA BanII 1296 GGGCTC G[GA]GC[CT]^C 1890 GGGCTC BsaWI 214 ACCGGT [AT]^CCGG[AT] 1924 TCCGGT BspHI 12 TCATGA T^CATGA 1406 TCATGA BspMI 708 ACCTGC ACCTGC(4/8) 742 GCAGGT BsrFI 214 ACCGGT [GA]^CCGG[CT] 1878 GCCGGC BstAPI 793 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1987 GCACGGGCTGC BstXI 1229 CCAAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1548 CCAATGGCCTGG BstYI 1304 AGATCC [GA]^GATC[CT] 1840 GGATCC Cfr10I 214 ACCGGT [GA]^CCGG[CT] 1878 GCCGGC DpnII 1305 GATC ^GATC 1841 GATC DsaI 952 CCACGG C^C[GA][CT]GG 1176 CCGTGG EaeI 1644 TGGCCA [CT]^GGCC[GA] 1942 TGGCCA EarI 1687 CTCTTC CTCTTC(1/4) 1869 GAAGAG HaeII 1024 GGCGCT [GA]GCGC^[CT] 1414 GGCGCC Ksp632I 1687 CTCTTC CTCTTC(1/4) 1869 GAAGAG MaeII 325 ACGT A^CGT 1328 ACGT MboI 1305 GATC ^GATC 1841 GATC MflI 1304 AGATCC [GA]^GATC[CT] 1840 GGATCC MluNI 1644 TGGCCA TGG^CCA 1942 TGGCCA MscI 1644 TGGCCA TGG^CCA 1942 TGGCCA MseI 329 TTAA T^TAA 593 TTAA NdeII 1305 GATC ^GATC 1841 GATC NspI 239 GCATGC [GA]CATG^[CT] 1970 GCATGC PflMI 888 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1011 CCAGCGGGTGG PstI 409 CTGCAG CTGCA^G 1148 CTGCAG RcaI 12 TCATGA T^CATGA 1406 TCATGA Sau3AI 1305 GATC ^GATC 1841 GATC SphI 239 GCATGC GCATG^C 1970 GCATGC Tru9I 329 TTAA T^TAA 593 TTAA Tsp45I 61 GTGAC ^GT[GC]AC 1856 GTGAC Van91I 888 CCAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1011 CCAGCGGGTGG XhoII 1304 AGATCC [GA]^GATC[CT] 1840 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaI AvaII BanI BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PleI PpuMI PvuII Sau96I ScrFI SfaNI SfcI StuI TseI Tsp509I TspRI XcmI