(Circular) (Six-base) MAPSORT of: pFN21AE5794.seq Check: 1417 from: 1 to: 6772 pFN21AE5794 6772 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2738 2738 Size: 6772 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4582 278 Size: 53 83 186 936 3046 2468 Fragments arranged by size: 3046 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2723 4115 1937 Size: 786 1392 4594 Fragments arranged by size: 4594 1392 786 Acc65I G'GTAC_C Cuts at: 4093 4093 Size: 6772 AclI AA'CG_TT Cuts at: 1563 2330 4899 5272 1563 Size: 767 2569 373 3063 Fragments arranged by size: 3063 2569 767 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2571 3470 3533 4835 6219 6487 1632 Size: 939 899 63 1302 1384 268 1917 Fragments arranged by size: 1917 1384 1302 939 899 268 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6575 AflIII A'CryG_T Cuts at: 1203 5671 6648 1203 Size: 4468 977 1327 Fragments arranged by size: 4468 1327 977 AgeI A'CCGG_T Cuts at: 2219 5603 2219 Size: 3384 3388 AhdI GACnn_n'nnGTC Cuts at: 5501 5501 Size: 6772 AleI CACnn'nnGTG Cuts at: 1166 3533 1166 Size: 2367 4405 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3281 3635 1184 Size: 2097 354 4321 Fragments arranged by size: 4321 2097 354 AlwNI CAG_nnn'CTG Cuts at: 2662 2804 2899 4515 6087 2662 Size: 142 95 1616 1572 3347 Fragments arranged by size: 3347 1616 1572 142 95 ApaLI G'TGCA_C Cuts at: 4829 5985 4829 Size: 1156 5616 ApoI r'AATT_y Cuts at: 1493 2836 4081 4307 4459 6568 1493 Size: 1343 1245 226 152 2109 1697 Fragments arranged by size: 2109 1697 1343 1245 226 152 AseI AT'TA_AT Cuts at: 160 1032 5326 160 Size: 872 4294 1606 Fragments arranged by size: 4294 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6772 AvaI C'yCGr_G Cuts at: 1870 1943 2142 3626 3816 3955 4097 1870 Size: 73 199 1484 190 139 142 4545 Fragments arranged by size: 4545 1484 199 190 142 139 73 BamHI G'GATC_C Cuts at: 3845 4102 4469 3845 Size: 257 367 6148 Fragments arranged by size: 6148 367 257 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2750 2902 3265 3419 4093 Size: 325 217 627 963 152 363 154 674 Cuts at: 4093 5548 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 963 674 627 363 325 217 154 152 BanII G_rGCy'C Cuts at: 1391 3305 3899 4091 1391 Size: 1914 594 192 4072 Fragments arranged by size: 4072 1914 594 192 BbeI G_GCGC'C Cuts at: 3423 3423 Size: 6772 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2337 928 Size: 971 438 5363 Fragments arranged by size: 5363 971 438 BbvCI CC'TCA_GC Cuts at: 2208 2208 Size: 6772 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4963 4997 1903 Size: 34 3026 34 3678 Fragments arranged by size: 3678 3026 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4664 5874 4664 Size: 1210 5562 BclI T'GATC_A Cuts at: 1548 1548 Size: 6772 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5383 136 Size: 107 122 71 631 605 3711 1525 Fragments arranged by size: 3711 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6767 6767 Size: 6772 BlpI GC'TnA_GC Cuts at: 2394 4189 2394 Size: 1795 4977 Bme1580I G_kGCm'C Cuts at: 1790 3270 3624 4833 5989 1790 Size: 1480 354 1209 1156 2573 Fragments arranged by size: 2573 1480 1209 1156 354 BmgBI CAC'GTC Cuts at: 1318 3334 1318 Size: 2016 4756 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2753 4503 5461 Size: 416 53 83 399 24 1336 1750 958 Cuts at: 5461 442 Size: 1753 Fragments arranged by size: 1753 1750 1336 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6772 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2104 2658 2727 3452 5432 1239 Size: 865 554 69 725 1980 2579 Fragments arranged by size: 2579 1980 865 725 554 69 Bpu10I CC'TnA_GC Cuts at: 2208 3936 2208 Size: 1728 5044 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3029 4897 5762 6060 3029 Size: 1868 865 298 3741 Fragments arranged by size: 3741 1868 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2049 2433 2815 5435 882 Size: 617 550 384 382 2620 2219 Fragments arranged by size: 2620 2219 617 550 384 382 BsaAI yAC'GTr Cuts at: 493 1204 4563 493 Size: 711 3359 2702 Fragments arranged by size: 3359 2702 711 BsaBI GATnn'nnATC Cuts at: 6766 6766 Size: 6772 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3420 4579 4961 5596 Size: 53 83 186 936 1887 1159 382 635 Cuts at: 5596 275 Size: 1451 Fragments arranged by size: 1887 1451 1159 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2219 3929 5205 5603 5877 6024 6435 2219 Size: 1710 1276 398 274 147 411 2556 Fragments arranged by size: 2556 1710 1276 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2043 2073 2102 2132 2169 Size: 30 165 30 640 30 29 30 37 Cuts at: 2169 2199 2768 2798 1178 Size: 30 569 30 5152 Fragments arranged by size: 5152 640 569 165 37 30 30 30 30 30 30 29 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2553 3379 4001 1186 Size: 1367 826 622 3957 Fragments arranged by size: 3957 1367 826 622 BseYI C'CCAG_C Cuts at: 2159 2717 3083 3805 3918 5975 6471 2159 Size: 558 366 722 113 2057 496 2460 Fragments arranged by size: 2460 2057 722 558 496 366 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3356 1163 Size: 573 1620 4579 Fragments arranged by size: 4579 1620 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3534 4222 4983 6011 1540 Size: 275 186 1533 688 761 1028 2301 Fragments arranged by size: 2301 1533 1028 761 688 275 186 BsiHKAI G_wGCw'C Cuts at: 3633 3793 3937 4833 4918 5989 3633 Size: 160 144 896 85 1071 4416 Fragments arranged by size: 4416 1071 896 160 144 85 BsmI GAATG_Cn' Cuts at: 2039 4283 4376 2039 Size: 2244 93 4435 Fragments arranged by size: 4435 2244 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3270 3305 3624 3633 3793 3899 3937 Size: 399 1480 35 319 9 160 106 38 Cuts at: 3937 4091 4833 4918 5989 1391 Size: 154 742 85 1071 2174 Fragments arranged by size: 2174 1480 1071 742 399 319 160 154 106 85 38 35 9 BspEI T'CCGG_A Cuts at: 6435 6435 Size: 6772 BspHI T'CATG_A Cuts at: 2017 3411 4661 6391 2017 Size: 1394 1250 1730 2398 Fragments arranged by size: 2398 1730 1394 1250 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2722 2738 4127 844 Size: 1878 16 1389 3489 Fragments arranged by size: 3489 1878 1389 16 BsrBI CCG'CTC Cuts at: 1394 1823 3504 4659 1394 Size: 429 1681 1155 3507 Fragments arranged by size: 3507 1681 1155 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3246 5267 5441 65 Size: 1183 1998 2021 174 1396 Fragments arranged by size: 2021 1998 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2219 3883 5416 5603 1700 Size: 110 409 1664 1533 187 2869 Fragments arranged by size: 2869 1664 1533 409 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6772 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6772 BssSI C'ACGA_G Cuts at: 2826 4832 5844 2826 Size: 2006 1012 3754 Fragments arranged by size: 3754 2006 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2804 3998 794 Size: 2010 1194 3568 Fragments arranged by size: 3568 2010 1194 BstBI TT'CG_AA Cuts at: 5576 5576 Size: 6772 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6772 BstXI CCAn_nnnn'nTGG Cuts at: 3241 3560 3241 Size: 319 6453 BstYI r'GATC_y Cuts at: 1972 3309 3845 4102 4469 4854 4871 6323 6409 Size: 1337 536 257 367 385 17 1452 86 Cuts at: 6409 6421 6767 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1337 536 385 367 346 257 86 17 12 BstZ17I GTA'TAC Cuts at: 2724 2724 Size: 6772 Bsu36I CC'TnA_GG Cuts at: 2772 2772 Size: 6772 BtgI C'CryG_G Cuts at: 513 1065 2957 3181 513 Size: 552 1892 224 4104 Fragments arranged by size: 4104 1892 552 224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6033 Fragments arranged by size: 6033 683 56 BtsI GCAGTG_nn' Cuts at: 3496 4288 5082 5102 3496 Size: 792 794 20 5166 Fragments arranged by size: 5166 794 792 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6772 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6737 DraI TTT'AAA Cuts at: 4076 4076 Size: 6772 DraIII CAC_nnn'GTG Cuts at: 2527 2527 Size: 6772 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3011 5779 809 Size: 507 1695 2768 1802 Fragments arranged by size: 2768 1802 1695 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3649 3947 4219 Size: 54 1425 50 275 18 1819 298 272 Cuts at: 4219 5108 6737 8 Size: 889 1629 43 Fragments arranged by size: 1819 1629 1425 889 298 275 272 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4219 1537 Size: 275 2407 4090 Fragments arranged by size: 4090 2407 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3698 3869 4702 1353 Size: 2345 171 833 3423 Fragments arranged by size: 3423 2345 833 171 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3694 5359 5733 5879 387 Size: 1064 138 2105 1665 374 146 1280 Fragments arranged by size: 2105 1665 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2104 2571 2658 2727 3452 3470 3533 Size: 393 472 467 87 69 725 18 63 Cuts at: 3533 4835 5432 6219 6487 1239 Size: 1302 597 787 268 1524 Fragments arranged by size: 1524 1302 787 725 597 472 467 393 268 87 69 63 18 EcoNI CCTnn'n_nnAGG Cuts at: 2745 2745 Size: 6772 EcoO109I rG'GnC_Cy Cuts at: 2991 3463 3809 2991 Size: 472 346 5954 Fragments arranged by size: 5954 472 346 EcoRI G'AATT_C Cuts at: 4081 4459 4081 Size: 378 6394 FspI TGC'GCA Cuts at: 3488 5278 3488 Size: 1790 4982 HaeII r_GCGC'y Cuts at: 1678 3033 3423 5919 1678 Size: 1355 390 2496 2531 Fragments arranged by size: 2531 2496 1355 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2169 2201 2768 2800 5413 Size: 32 135 32 597 32 567 32 2613 Cuts at: 5413 5445 5487 5519 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2613 597 567 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4116 4362 669 Size: 988 281 2178 246 3079 Fragments arranged by size: 3079 2178 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6772 HpaI GTT'AAC Cuts at: 4362 4362 Size: 6772 KasI G'GCGC_C Cuts at: 3419 3419 Size: 6772 KpnI G_GTAC'C Cuts at: 4097 4097 Size: 6772 MfeI C'AATT_G Cuts at: 2633 4371 2633 Size: 1738 5034 MluI A'CGCG_T Cuts at: 6648 6648 Size: 6772 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2660 5886 6070 1305 Size: 1355 3226 184 2007 Fragments arranged by size: 3226 2007 1355 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3651 3949 10 Size: 54 1425 343 1819 298 2833 Fragments arranged by size: 2833 1819 1425 343 298 54 MslI CAynn'nnrTG Cuts at: 518 1166 3123 3533 3617 4730 5089 5248 518 Size: 648 1957 410 84 1113 359 159 2042 Fragments arranged by size: 2042 1957 1113 648 410 359 159 84 MspA1I CmG'CkG Cuts at: 1606 2097 2662 2801 3019 3433 3786 3805 4188 Size: 491 565 139 218 414 353 19 383 Cuts at: 4188 4865 6013 6258 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 565 491 414 383 353 245 218 139 19 NaeI GCC'GGC Cuts at: 3885 3885 Size: 6772 NarI GG'CG_CC Cuts at: 3420 3420 Size: 6772 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6220 NdeI CA'TA_TG Cuts at: 387 2533 387 Size: 2146 4626 NgoMIV G'CCGG_C Cuts at: 3883 3883 Size: 6772 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6772 NotI GC'GGCC_GC Cuts at: 4219 4219 Size: 6772 NspI r_CATG'y Cuts at: 1132 1229 2248 3979 4130 5675 1132 Size: 97 1019 1731 151 1545 2229 Fragments arranged by size: 2229 1731 1545 1019 151 97 PasI CC'CwG_GG Cuts at: 1348 3674 1348 Size: 2326 4446 PciI A'CATG_T Cuts at: 5671 5671 Size: 6772 PflMI CCAn_nnn'nTGG Cuts at: 1108 2899 3022 1108 Size: 1791 123 4858 Fragments arranged by size: 4858 1791 123 PmeI GTTT'AAAC Cuts at: 4076 4076 Size: 6772 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 4841 4873 6354 6386 3752 Size: 32 1057 32 1481 32 4138 Fragments arranged by size: 4138 1481 1057 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2991 3463 3809 2991 Size: 472 346 5954 Fragments arranged by size: 5954 472 346 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6772 PsiI TTA'TAA Cuts at: 4342 4342 Size: 6772 PstI C_TGCA'G Cuts at: 830 2418 3157 4124 830 Size: 1588 739 967 3478 Fragments arranged by size: 3478 1588 967 739 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6772 PvuII CAG'CTG Cuts at: 2097 2801 3433 3786 3805 2097 Size: 704 632 353 19 5064 Fragments arranged by size: 5064 704 632 353 19 SalI G'TCGA_C Cuts at: 1936 4114 1936 Size: 2178 4594 SanDI GG'GwC_CC Cuts at: 2991 3809 2991 Size: 818 5954 SapI GCTCTTCn'nnn_ Cuts at: 3869 3869 Size: 6772 SbfI CC_TGCA'GG Cuts at: 4124 4124 Size: 6772 ScaI AGT'ACT Cuts at: 2653 5020 6732 2653 Size: 2367 1712 2693 Fragments arranged by size: 2693 2367 1712 SexAI A'CCwGG_T Cuts at: 3261 3261 Size: 6772 SfcI C'TryA_G Cuts at: 826 1045 2414 3153 3716 4120 5255 5936 6127 Size: 219 1369 739 563 404 1135 681 191 Cuts at: 6127 826 Size: 1471 Fragments arranged by size: 1471 1369 1135 739 681 563 404 219 191 SfoI GGC'GCC Cuts at: 3421 3421 Size: 6772 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6772 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6772 SmaI CCC'GGG Cuts at: 1872 4099 1872 Size: 2227 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3008 4876 5777 6039 820 Size: 197 926 1065 1868 901 262 1553 Fragments arranged by size: 1868 1553 1065 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4563 493 Size: 4070 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6772 SphI G_CATG'C Cuts at: 1132 2248 3979 4130 1132 Size: 1116 1731 151 3774 Fragments arranged by size: 3774 1731 1116 151 SspI AAT'ATT Cuts at: 5 52 4696 5 Size: 47 4644 2081 Fragments arranged by size: 4644 2081 47 StuI AGG'CCT Cuts at: 1517 3288 3826 3941 1517 Size: 1771 538 115 4348 Fragments arranged by size: 4348 1771 538 115 StyI C'CwwG_G Cuts at: 513 1065 4211 513 Size: 552 3146 3074 Fragments arranged by size: 3146 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4799 4816 4969 5154 5599 325 Size: 1255 3219 17 153 185 445 1498 Fragments arranged by size: 3219 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2651 5018 6730 Size: 274 80 33 51 1443 674 2367 1712 Cuts at: 6730 96 Size: 138 Fragments arranged by size: 2367 1712 1443 674 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2102 2134 2448 2480 3386 3418 6732 6764 2102 Size: 32 314 32 906 32 3314 32 2110 Fragments arranged by size: 3314 2110 906 314 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6772 XbaI T'CTAG_A Cuts at: 4108 4108 Size: 6772 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2077 2188 3023 3259 3430 2077 Size: 111 835 236 171 5419 Fragments arranged by size: 5419 835 236 171 111 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6772 XmaI C'CCGG_G Cuts at: 1870 4097 1870 Size: 2227 4545 XmnI GAAnn'nnTTC Cuts at: 2037 4901 2037 Size: 2864 3908 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4580 276 Size: 53 83 186 936 3046 2468 Fragments arranged by size: 3046 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI SrfI SwaI