(Linear) (Six-base) MAPSORT of: KIEE5794.seq Check: 3102 from: 1 to: 2067 KIEE5794.orf With 182 enzymes: SgfI * December 5, 2014 13:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 733 2067 Size: 733 1334 AccI GT'mk_AC Cuts at: 0 718 2067 Size: 718 1349 AclI AA'CG_TT Cuts at: 0 325 2067 Size: 325 1742 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 566 1465 1528 2067 Size: 566 899 63 539 Fragments arranged by size: 899 566 539 63 AgeI A'CCGG_T Cuts at: 0 214 2067 Size: 214 1853 AleI CACnn'nnGTG Cuts at: 0 1528 2067 Size: 1528 539 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1276 1630 2067 Size: 1276 354 437 Fragments arranged by size: 1276 437 354 AlwNI CAG_nnn'CTG Cuts at: 0 657 799 894 2067 Size: 657 142 95 1173 Fragments arranged by size: 1173 657 142 95 ApoI r'AATT_y Cuts at: 0 831 2067 Size: 831 1236 AvaI C'yCGr_G Cuts at: 0 137 1621 1811 1950 2067 Size: 137 1484 190 139 117 Fragments arranged by size: 1484 190 139 137 117 BamHI G'GATC_C Cuts at: 0 1840 2067 Size: 1840 227 BanI G'GyrC_C Cuts at: 0 745 897 1260 1414 2067 Size: 745 152 363 154 653 Fragments arranged by size: 745 653 363 154 152 BanII G_rGCy'C Cuts at: 0 1300 1894 2067 Size: 1300 594 173 Fragments arranged by size: 1300 594 173 BbeI G_GCGC'C Cuts at: 0 1418 2067 Size: 1418 649 BbsI GAAGACnn'nnnn_ Cuts at: 0 332 2067 Size: 332 1735 BbvCI CC'TCA_GC Cuts at: 0 203 2067 Size: 203 1864 BlpI GC'TnA_GC Cuts at: 0 389 2067 Size: 389 1678 Bme1580I G_kGCm'C Cuts at: 0 1265 1619 2067 Size: 1265 354 448 Fragments arranged by size: 1265 448 354 BmgBI CAC'GTC Cuts at: 0 1329 2067 Size: 1329 738 BmrI ACTGGGnnnn_n' Cuts at: 0 748 2067 Size: 748 1319 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 99 653 722 1447 2067 Size: 99 554 69 725 620 Fragments arranged by size: 725 620 554 99 69 Bpu10I CC'TnA_GC Cuts at: 0 203 1931 2067 Size: 203 1728 136 Fragments arranged by size: 1728 203 136 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1024 2067 Size: 1024 1043 BsaI GGTCTCn'nnnn_ Cuts at: 0 44 428 810 2067 Size: 44 384 382 1257 Fragments arranged by size: 1257 384 382 44 BsaHI Gr'CG_yC Cuts at: 0 1415 2067 Size: 1415 652 BsaWI w'CCGG_w Cuts at: 0 214 1924 2067 Size: 214 1710 143 Fragments arranged by size: 1710 214 143 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 38 68 97 127 164 194 763 793 Size: 38 30 29 30 37 30 569 30 Cuts at: 793 2067 Size: 1274 Fragments arranged by size: 1274 569 38 37 30 30 30 30 29 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 548 1374 1996 2067 Size: 548 826 622 71 Fragments arranged by size: 826 622 548 71 BseYI C'CCAG_C Cuts at: 0 154 712 1078 1800 1913 2067 Size: 154 558 366 722 113 154 Fragments arranged by size: 722 558 366 154 154 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1351 2067 Size: 1351 716 BsiEI CG_ry'CG Cuts at: 0 1529 2067 Size: 1529 538 BsiHKAI G_wGCw'C Cuts at: 0 1628 1788 1932 2067 Size: 1628 160 144 135 Fragments arranged by size: 1628 160 144 135 BsmI GAATG_Cn' Cuts at: 0 34 2067 Size: 34 2033 Bsp1286I G_dGCh'C Cuts at: 0 1265 1300 1619 1628 1788 1894 1932 2067 Size: 1265 35 319 9 160 106 38 135 Fragments arranged by size: 1265 319 160 135 106 38 35 9 BspHI T'CATG_A Cuts at: 0 12 1406 2067 Size: 12 1394 661 Fragments arranged by size: 1394 661 12 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 717 733 2067 Size: 717 16 1334 Fragments arranged by size: 1334 717 16 BsrBI CCG'CTC Cuts at: 0 1499 2067 Size: 1499 568 BsrDI GCAATG_nn' Cuts at: 0 1241 2067 Size: 1241 826 BsrFI r'CCGG_y Cuts at: 0 214 1878 2067 Size: 214 1664 189 Fragments arranged by size: 1664 214 189 BssSI C'ACGA_G Cuts at: 0 821 2067 Size: 821 1246 BstAPI GCAn_nnn'nTGC Cuts at: 0 799 1993 2067 Size: 799 1194 74 Fragments arranged by size: 1194 799 74 BstXI CCAn_nnnn'nTGG Cuts at: 0 1236 1555 2067 Size: 1236 319 512 Fragments arranged by size: 1236 512 319 BstYI r'GATC_y Cuts at: 0 1304 1840 2067 Size: 1304 536 227 Fragments arranged by size: 1304 536 227 BstZ17I GTA'TAC Cuts at: 0 719 2067 Size: 719 1348 Bsu36I CC'TnA_GG Cuts at: 0 767 2067 Size: 767 1300 BtgI C'CryG_G Cuts at: 0 952 1176 2067 Size: 952 224 891 Fragments arranged by size: 952 891 224 BtsI GCAGTG_nn' Cuts at: 0 1491 2067 Size: 1491 576 DraIII CAC_nnn'GTG Cuts at: 0 522 2067 Size: 522 1545 DrdI GACnn_nn'nnGTC Cuts at: 0 1006 2067 Size: 1006 1061 EaeI y'GGCC_r Cuts at: 0 1644 1942 2067 Size: 1644 298 125 Fragments arranged by size: 1644 298 125 EarI CTCTTCn'nnn_ Cuts at: 0 1693 1864 2067 Size: 1693 171 203 Fragments arranged by size: 1693 203 171 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1689 2067 Size: 1689 378 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 99 566 653 722 1447 1465 1528 2067 Size: 99 467 87 69 725 18 63 539 Fragments arranged by size: 725 539 467 99 87 69 63 18 EcoNI CCTnn'n_nnAGG Cuts at: 0 740 2067 Size: 740 1327 EcoO109I rG'GnC_Cy Cuts at: 0 986 1458 1804 2067 Size: 986 472 346 263 Fragments arranged by size: 986 472 346 263 FspI TGC'GCA Cuts at: 0 1483 2067 Size: 1483 584 HaeII r_GCGC'y Cuts at: 0 1028 1418 2067 Size: 1028 390 649 Fragments arranged by size: 1028 649 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 164 196 763 795 2067 Size: 164 32 567 32 1272 Fragments arranged by size: 1272 567 164 32 32 KasI G'GCGC_C Cuts at: 0 1414 2067 Size: 1414 653 MfeI C'AATT_G Cuts at: 0 628 2067 Size: 628 1439 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 655 2067 Size: 655 1412 MscI TGG'CCA Cuts at: 0 1646 1944 2067 Size: 1646 298 123 Fragments arranged by size: 1646 298 123 MslI CAynn'nnrTG Cuts at: 0 1118 1528 1612 2067 Size: 1118 410 84 455 Fragments arranged by size: 1118 455 410 84 MspA1I CmG'CkG Cuts at: 0 92 657 796 1014 1428 1781 1800 2067 Size: 92 565 139 218 414 353 19 267 Fragments arranged by size: 565 414 353 267 218 139 92 19 NaeI GCC'GGC Cuts at: 0 1880 2067 Size: 1880 187 NarI GG'CG_CC Cuts at: 0 1415 2067 Size: 1415 652 NdeI CA'TA_TG Cuts at: 0 528 2067 Size: 528 1539 NgoMIV G'CCGG_C Cuts at: 0 1878 2067 Size: 1878 189 NspI r_CATG'y Cuts at: 0 243 1974 2067 Size: 243 1731 93 Fragments arranged by size: 1731 243 93 PasI CC'CwG_GG Cuts at: 0 1669 2067 Size: 1669 398 PflMI CCAn_nnn'nTGG Cuts at: 0 894 1017 2067 Size: 894 123 1050 Fragments arranged by size: 1050 894 123 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1747 1779 2067 Size: 1747 32 288 Fragments arranged by size: 1747 288 32 PpuMI rG'GwC_Cy Cuts at: 0 986 1458 1804 2067 Size: 986 472 346 263 Fragments arranged by size: 986 472 346 263 PstI C_TGCA'G Cuts at: 0 413 1152 2067 Size: 413 739 915 Fragments arranged by size: 915 739 413 PvuII CAG'CTG Cuts at: 0 92 796 1428 1781 1800 2067 Size: 92 704 632 353 19 267 Fragments arranged by size: 704 632 353 267 92 19 SanDI GG'GwC_CC Cuts at: 0 986 1804 2067 Size: 986 818 263 Fragments arranged by size: 986 818 263 SapI GCTCTTCn'nnn_ Cuts at: 0 1864 2067 Size: 1864 203 ScaI AGT'ACT Cuts at: 0 648 2067 Size: 648 1419 SexAI A'CCwGG_T Cuts at: 0 1256 2067 Size: 1256 811 SfcI C'TryA_G Cuts at: 0 409 1148 1711 2067 Size: 409 739 563 356 Fragments arranged by size: 739 563 409 356 SfoI GGC'GCC Cuts at: 0 1416 2067 Size: 1416 651 SmlI C'TyrA_G Cuts at: 0 1003 2067 Size: 1003 1064 SphI G_CATG'C Cuts at: 0 243 1974 2067 Size: 243 1731 93 Fragments arranged by size: 1731 243 93 StuI AGG'CCT Cuts at: 0 1283 1821 1936 2067 Size: 1283 538 115 131 Fragments arranged by size: 1283 538 131 115 TatI w'GTAC_w Cuts at: 0 646 2067 Size: 646 1421 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 97 129 443 475 1381 1413 2067 Size: 97 32 314 32 906 32 654 Fragments arranged by size: 906 654 314 97 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 72 183 1018 1254 1425 2067 Size: 72 111 835 236 171 642 Fragments arranged by size: 835 642 236 171 111 72 XmnI GAAnn'nnTTC Cuts at: 0 32 2067 Size: 32 2035 Enzymes that do cut: AarI AccI AclI AcuI AgeI AleI AlfI AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PasI PflMI PpiI PpuMI PstI PvuII SanDI SapI ScaI SexAI SfcI SfoI SmlI SphI StuI TatI TstI XcmI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AflIII AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglI BglII BmtI BplI BsaAI BsaBI BsiWI BsmBI BspEI BsrGI BssHII BstBI BstEII BtgZI ClaI CspCI DraI EagI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MluI NcoI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StyI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI