Restriction map for
pFN21AE2264
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AspI
AvaII AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BclI BcnI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmFI Bsp1286I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BssHII Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DdeI DraI DraII Eco47III
Eco81I EcoNI EcoO109I EcoRV EcoT22I
FbaI FseI FspI HaeII HapII
HindIII HpaI HpaII KasI KpnI
KspI MaeII MluI MroI MslI
MspA1I MspI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SapI
Sau96I SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Tth111I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 835 GTCGAC GT^[AC][GT]AC
AccII 79 CGCG CG^CG
AcsI 898 GAATTC [GA]^AATT[CT]
AcyI 740 GACGCC G[GA]^CG[CT]C
AfaI 852 GTAC GT^AC
AhdI 261 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 898 GAATTC [GA]^AATT[CT]
Asp700I 331 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 261 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2 TGGCCA TGG^CCA
BanI 82 GGCACC G^G[CT][GA]CC
BbsI 258 GAAGAC GAAGAC(2/6)
BcgI 383 CGAGAAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 303 GGATAC GTATCC(6/5)
BpuAI 258 GAAGAC GAAGAC(2/6)
BsaBI 328 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 740 GACGCC G[GA]^CG[CT]C
BsgI 391 CTGCAC GTGCAG(16/14)
BsiEI 830 CGGCCG CG[GA][CT]^CG
BsmBI 738 GAGACG CGTCTC(1/5)
BsmI 441 GCATTC GAATGC(1/-1)
Bsp1407I 851 TGTACA T^GTACA
BspHI 719 TCATGA T^CATGA
BsrGI 851 TGTACA T^GTACA
BssSI 381 CACGAG CACGAG(-5/-1)
BstEII 860 GGTTACC G^GT[ACGT]ACC
BstPI 860 GGTTACC G^GT[ACGT]ACC
BstUI 79 CGCG CG^CG
CfoI 78 GCGC GCG^C
DraIII 157 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 768 GACCTGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 222 CCATGG C^C[GA][CT]GG
EagI 830 CGGCCG C^GGCCG
Eam1105I 261 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 535 GAAGAG CTCTTC(1/4)
EclXI 830 CGGCCG C^GGCCG
Eco52I 830 CGGCCG C^GGCCG
EcoO65I 860 GGTTACC G^GT[ACGT]ACC
EcoRI 898 GAATTC G^AATTC
FauI 757 GCGGG CCCGC(4/6)
HhaI 78 GCGC GCG^C
Hin1I 740 GACGCC G[GA]^CG[CT]C
HinP1I 78 GCGC G^CGC
Ksp632I 535 GAAGAG CTCTTC(1/4)
MamI 328 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 568 CAATTG C^AATTG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 322 TTAA T^TAA
MunI 568 CAATTG C^AATTG
MvnI 79 CGCG CG^CG
NcoI 222 CCATGG C^CATGG
PflMI 308 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 719 TCATGA T^CATGA
RsaI 852 GTAC GT^AC
SalI 835 GTCGAC G^TCGAC
SspBI 851 TGTACA T^GTACA
SspI 350 AATATT AAT^ATT
Tru9I 322 TTAA T^TAA
TspRI 745 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 308 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 432 TCTAGA T^CTAGA
XmnI 331 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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BfaI 8 CTAG C^TAG
433 CTAG
BglII 333 AGATCT A^GATCT
510 AGATCT
BsaI 518 GAGACC GGTCTC(1/5)
766 GAGACC
BsaJI 222 CCATGG C^C[ACGT][ACGT]GG
288 CCTTGG
BssKI 753 CCTGG ^CC[ACGT]GG
903 CCTGG
BstAPI 398 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
927 GCAGCTCATGC
BstNI 753 CCTGG CC^[AT]GG
903 CCTGG
BstYI 333 AGATCT [GA]^GATC[CT]
510 AGATCT
EaeI 2 TGGCCA [CT]^GGCC[GA]
830 CGGCCG
EcoRII 753 CCTGG ^CC[AT]GG
903 CCTGG
EcoT14I 222 CCATGG C^C[AT][AT]GG
288 CCTTGG
HaeIII 3 GGCC GG^CC
831 GGCC
HgaI 58 GACGC GACGC(5/10)
740 GACGC
MaeI 8 CTAG C^TAG
433 CTAG
MflI 333 AGATCT [GA]^GATC[CT]
510 AGATCT
MvaI 753 CCTGG CC^[AT]GG
903 CCTGG
NlaIV 82 GGCACC GG[ACGT]^[ACGT]CC
800 GGCTCC
ScrFI 753 CCTGG CC^[ACGT]GG
903 CCTGG
StyI 222 CCATGG C^C[AT][AT]GG
288 CCTTGG
XhoII 333 AGATCT [GA]^GATC[CT]
510 AGATCT
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AvaI BbvI BpmI
BsiYI BslI BsmAI BsoBI BspMI
BsrI BstF5I Cac8I DpnII Fnu4HI
FokI HincII HindII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MwoI NdeII NlaIII PaeR7I PleI
Sau3AI SfaNI SmlI TaqI TfiI
TseI Tsp45I Tsp509I TthHB8I XhoI