(Circular) (Six-base) MAPSORT of: pFN21AE2264.seq Check: 3691 from: 1 to: 5707 pFN21AE2264 5707 bp With 182 enzymes: SgfI * March 28, 2012 12:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2099 2099 Size: 5707 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2841 3050 1937 Size: 904 209 4594 Fragments arranged by size: 4594 904 209 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2447 2663 2898 3011 3770 5154 5422 1632 Size: 815 216 235 113 759 1384 268 1917 Fragments arranged by size: 1917 1384 815 759 268 235 216 113 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 2271 4436 2271 Size: 2165 3542 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AlwNI CAG_nnn'CTG Cuts at: 3450 5022 3450 Size: 1572 4135 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 2903 3016 3242 3394 5503 1493 Size: 1410 113 226 152 2109 1697 Fragments arranged by size: 2109 1697 1410 226 152 113 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2053 2220 2871 3032 1870 Size: 73 110 167 651 161 4545 Fragments arranged by size: 4545 651 167 161 110 73 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 3028 4483 618 Size: 325 217 627 300 941 1455 1842 Fragments arranged by size: 1842 1455 941 627 325 300 217 BanII G_rGCy'C Cuts at: 1391 3026 1391 Size: 1635 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2270 928 Size: 971 371 4365 Fragments arranged by size: 4365 971 371 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2377 2411 3898 3932 1903 Size: 34 440 34 1487 34 3678 Fragments arranged by size: 3678 1487 440 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2302 3599 4809 2302 Size: 1297 1210 3200 Fragments arranged by size: 3200 1297 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2338 2515 5702 2338 Size: 177 3187 2343 Fragments arranged by size: 3187 2343 177 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 3768 4924 1790 Size: 1978 1156 2573 Fragments arranged by size: 2573 1978 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3438 4396 442 Size: 416 53 83 399 24 2021 958 1753 Fragments arranged by size: 2021 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2534 2534 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2341 2604 2948 4367 1239 Size: 1102 263 344 1419 2579 Fragments arranged by size: 2579 1419 1102 344 263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3832 4697 4995 3832 Size: 865 298 4544 Fragments arranged by size: 4544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2517 2765 4370 882 Size: 617 1018 248 1605 2219 Fragments arranged by size: 2219 1605 1018 617 248 BsaAI yAC'GTr Cuts at: 493 1204 3498 493 Size: 711 2294 2702 Fragments arranged by size: 2702 2294 711 BsaBI GATnn'nnATC Cuts at: 2337 5701 2337 Size: 3364 2343 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2746 3514 3896 4531 Size: 53 83 186 936 1213 768 382 635 Cuts at: 4531 275 Size: 1451 Fragments arranged by size: 1451 1213 936 768 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5482 Fragments arranged by size: 5482 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5707 BseYI C'CCAG_C Cuts at: 2958 4910 5406 2958 Size: 1952 496 3259 Fragments arranged by size: 3259 1952 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 1163 Size: 573 645 4489 Fragments arranged by size: 4489 645 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2838 3157 3918 4946 1540 Size: 275 186 837 319 761 1028 2301 Fragments arranged by size: 2301 1028 837 761 319 275 186 BsiHKAI G_wGCw'C Cuts at: 3768 3853 4924 3768 Size: 85 1071 4551 Fragments arranged by size: 4551 1071 85 BsmI GAATG_Cn' Cuts at: 2446 3218 3311 2446 Size: 772 93 4842 Fragments arranged by size: 4842 772 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2737 2737 Size: 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3026 3768 3853 4924 1391 Size: 399 1236 742 85 1071 2174 Fragments arranged by size: 2174 1236 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 2724 3596 5326 2724 Size: 872 1730 3105 Fragments arranged by size: 3105 1730 872 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2099 2745 2783 3062 844 Size: 1255 646 38 279 3489 Fragments arranged by size: 3489 1255 646 279 38 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4202 4376 65 Size: 1183 2954 174 1396 Fragments arranged by size: 2954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4351 4538 1700 Size: 110 2541 187 2869 Fragments arranged by size: 2869 2541 187 110 BsrGI T'GTAC_A Cuts at: 96 2856 96 Size: 2760 2947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 2386 3767 4779 2386 Size: 1381 1012 3314 Fragments arranged by size: 3314 1381 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2409 2938 794 Size: 1615 529 3563 Fragments arranged by size: 3563 1615 529 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 2865 1184 Size: 1681 4026 BstYI r'GATC_y Cuts at: 1972 2338 2515 3037 3404 3789 3806 5258 5344 Size: 366 177 522 367 385 17 1452 86 Cuts at: 5344 5356 5702 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 522 385 367 366 346 177 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2227 513 Size: 552 939 223 3993 Fragments arranged by size: 3993 939 552 223 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 3223 4017 4037 3223 Size: 794 20 4893 Fragments arranged by size: 4893 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DraIII CAC_nnn'GTG Cuts at: 2167 2167 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2779 4714 809 Size: 507 1463 1935 1802 Fragments arranged by size: 1935 1802 1463 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2835 3154 Size: 54 1425 50 275 18 177 828 319 Cuts at: 3154 4043 5672 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 828 319 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2835 3154 1537 Size: 275 1023 319 4090 Fragments arranged by size: 4090 1023 319 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2535 3637 1353 Size: 1182 1102 3423 Fragments arranged by size: 3423 1182 1102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2341 2447 2604 2663 2898 2948 3011 Size: 393 709 106 157 59 235 50 63 Cuts at: 3011 3770 4367 5154 5422 1239 Size: 759 597 787 268 1524 Fragments arranged by size: 1524 787 759 709 597 393 268 235 157 106 63 59 50 EcoRI G'AATT_C Cuts at: 2903 3016 3394 2903 Size: 113 378 5216 Fragments arranged by size: 5216 378 113 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 4854 1678 Size: 3176 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2257 2289 4348 4380 4422 Size: 32 135 32 685 32 2059 32 42 Cuts at: 4422 4454 1373 Size: 32 2626 Fragments arranged by size: 2626 2059 685 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2290 2368 2842 3051 3297 669 Size: 988 281 352 78 474 209 246 3079 Fragments arranged by size: 3079 988 474 352 281 246 209 78 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 2573 3306 2573 Size: 733 4974 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4821 5005 1305 Size: 3516 184 2007 Fragments arranged by size: 3516 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3708 Fragments arranged by size: 3708 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3665 4024 4183 518 Size: 648 2499 359 159 2042 Fragments arranged by size: 2499 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3123 3800 4948 5193 1606 Size: 1517 677 1148 245 2120 Fragments arranged by size: 2120 1517 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2227 513 Size: 552 939 223 3993 Fragments arranged by size: 3993 939 552 223 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 2319 1108 Size: 1211 4496 PfoI T'CCnGG_A Cuts at: 2907 2907 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 2246 3277 2246 Size: 1031 4676 PspXI vC'TCGA_Gb Cuts at: 2053 2220 2871 2053 Size: 167 651 4889 Fragments arranged by size: 4889 651 167 PstI C_TGCA'G Cuts at: 830 3059 830 Size: 2229 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 SalI G'TCGA_C Cuts at: 1936 2840 3049 1936 Size: 904 209 4594 Fragments arranged by size: 4594 904 209 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SfcI C'TryA_G Cuts at: 826 1045 3055 4190 4871 5062 826 Size: 219 2010 1135 681 191 1471 Fragments arranged by size: 2010 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2053 2220 2871 3811 4712 4974 Size: 197 926 110 167 651 940 901 262 Cuts at: 4974 820 Size: 1553 Fragments arranged by size: 1553 940 926 901 651 262 197 167 110 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 2357 3631 5 Size: 47 2305 1274 2081 Fragments arranged by size: 2305 2081 1274 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2004 2227 2293 3146 513 Size: 552 939 223 66 853 3074 Fragments arranged by size: 3074 939 853 552 223 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2693 2808 3734 3751 3904 4089 4534 Size: 1255 1113 115 926 17 153 185 445 Cuts at: 4534 325 Size: 1498 Fragments arranged by size: 1498 1255 1113 926 445 185 153 115 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2856 3953 5665 Size: 274 80 33 51 1443 879 1097 1712 Cuts at: 5665 96 Size: 138 Fragments arranged by size: 1712 1443 1097 879 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2889 2921 5667 5699 2889 Size: 32 2746 32 2897 Fragments arranged by size: 2897 2746 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 2437 3043 2437 Size: 606 5101 XhoI C'TCGA_G Cuts at: 1943 2053 2220 2871 1943 Size: 110 167 651 4779 Fragments arranged by size: 4779 651 167 110 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 2340 3836 2340 Size: 1496 4211 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI