Restriction map for
pFN21AE0761
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI Eam1105I EarI Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HindIII HpaI
KasI KpnI Ksp632I MaeI MamI
MfeI MluI MroI MseI MunI
NarI NcoI NdeI NheI NruI
NsiI NspI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Tth111I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 181 GGCGTC G[GA]^CG[CT]C
AlwNI 861 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 424 GGGCCC GGGCC^C
BalI 638 TGGCCA TGG^CCA
BbrPI 739 CACGTG CAC^GTG
BbvCI 628 GCTGAGG CCTCAGC(-5/-2)
BglI 15 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 739 CACGTG [CT]AC^GT[GA]
BsaHI 181 GGCGTC G[GA]^CG[CT]C
BsgI 73 CTGCAC GTGCAG(16/14)
BsmBI 199 GAGACG CGTCTC(1/5)
BstXI 789 CCAGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 876 AGATCC [GA]^GATC[CT]
DraIII 208 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 160 GACCGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 926 CCAAGG C^C[AT][AT]GG
Hin1I 181 GGCGTC G[GA]^CG[CT]C
KspI 93 CCGCGG CCGC^GG
MboII 998 TCTTC GAAGA(8/7)
MflI 876 AGATCC [GA]^GATC[CT]
MluNI 638 TGGCCA TGG^CCA
MscI 638 TGGCCA TGG^CCA
MslI 588 CATTGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 290 GCCGGC GCC^GGC
NgoMIV 290 GCCGGC G^CCGGC
NotI 21 GCGGCCGC GC^GGCCGC
PaeR7I 938 CTCGAG C^TCGAG
PflMI 207 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 739 CACGTG CAC^GTG
PmlI 739 CACGTG CAC^GTG
PvuII 298 CAGCTG CAG^CTG
SacI 283 GAGCTC GAGCT^C
SacII 93 CCGCGG CCGC^GG
SexAI 209 ACCAGGT A^CC[AT]GGT
SfaNI 644 GCATC GCATC(5/9)
SgrAI 289 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 540 CCCGGG CCC^GGG
SmlI 938 CTCGAG C^T[CT][GA]AG
StyI 926 CCAAGG C^C[AT][AT]GG
Van91I 207 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 938 CTCGAG C^TCGAG
XhoII 876 AGATCC [GA]^GATC[CT]
XmaCI 540 CCCGGG C^CCGGG
XmaI 540 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 308 GTAC GT^AC
522 GTAC
AlwI 877 GATCC GGATC(4/5)
224 GGATC
AocI 429 CCTGAGG CC^T[ACGT]AGG
472 CCTCAGG
BsiEI 22 CGGCCG CG[GA][CT]^CG
917 CGGCCG
Bsu36I 429 CCTGAGG CC^T[ACGT]AGG
472 CCTCAGG
DpnII 225 GATC ^GATC
877 GATC
DsaI 93 CCGCGG C^C[GA][CT]GG
911 CCACGG
EagI 22 CGGCCG C^GGCCG
917 CGGCCG
EclXI 22 CGGCCG C^GGCCG
917 CGGCCG
Eco52I 22 CGGCCG C^GGCCG
917 CGGCCG
Eco81I 429 CCTGAGG CC^T[ACGT]AGG
472 CCTCAGG
EcoNI 165 CCTGGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
459 CCTGCGAAAGG
HgaI 201 GACGC GACGC(5/10)
182 GCGTC
HinfI 246 GACTC G^A[ACGT]TC
950 GAGTC
MaeII 188 ACGT A^CGT
740 ACGT
MboI 225 GATC ^GATC
877 GATC
NdeII 225 GATC ^GATC
877 GATC
NlaIII 625 CATG CATG^
945 CATG
PleI 246 GACTC GAGTC(4/5)
950 GAGTC
PpuMI 468 GGGACCT [GA]G^G[AT]CC[CT]
747 AGGTCCT
RsaI 308 GTAC GT^AC
522 GTAC
Sau3AI 225 GATC ^GATC
877 GATC
TaqI 170 TCGA T^CGA
939 TCGA
TthHB8I 170 TCGA T^CGA
939 TCGA
XcmI 789 CCAGCTCAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
967 CCACAGATGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HpaII HphI ItaI MaeIII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV Sau96I ScrFI
TseI Tsp45I TspRI