(Circular) (Six-base) MAPSORT of: pFN21AE0761.seq Check: 9563 from: 1 to: 5716 pFN21AE0761 5716 bp With 182 enzymes: SgfI * October 11, 2011 14:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2085 2472 2563 2085 Size: 387 91 5238 Fragments arranged by size: 5238 387 91 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3526 278 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3059 1937 Size: 1122 4594 Acc65I G'GTAC_C Cuts at: 3037 3037 Size: 5716 AclI AA'CG_TT Cuts at: 1563 3843 4216 1563 Size: 2280 373 3063 Fragments arranged by size: 3063 2280 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2989 3779 5163 5431 1632 Size: 1357 790 1384 268 1917 Fragments arranged by size: 1917 1384 1357 790 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5519 AflIII A'CryG_T Cuts at: 1203 4615 5592 1203 Size: 3412 977 1327 Fragments arranged by size: 3412 1327 977 AgeI A'CCGG_T Cuts at: 4547 4547 Size: 5716 AhdI GACnn_n'nnGTC Cuts at: 4445 4445 Size: 5716 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5716 AlwNI CAG_nnn'CTG Cuts at: 2871 3459 5031 2871 Size: 588 1572 3556 Fragments arranged by size: 3556 1572 588 ApaI G_GGCC'C Cuts at: 2433 2433 Size: 5716 ApaLI G'TGCA_C Cuts at: 3773 4929 3773 Size: 1156 4560 ApoI r'AATT_y Cuts at: 1493 3025 3251 3403 5512 1493 Size: 1532 226 152 2109 1697 Fragments arranged by size: 2109 1697 1532 226 152 AseI AT'TA_AT Cuts at: 160 1032 4270 160 Size: 872 3238 1606 Fragments arranged by size: 3238 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 AvaI C'yCGr_G Cuts at: 1870 1943 2545 2693 2943 3041 1870 Size: 73 602 148 250 98 4545 Fragments arranged by size: 4545 602 250 148 98 73 BamHI G'GATC_C Cuts at: 3046 3413 3046 Size: 367 5349 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3037 4492 618 Size: 325 217 627 1250 1455 1842 Fragments arranged by size: 1842 1455 1250 627 325 217 BanII G_rGCy'C Cuts at: 1391 2292 2340 2433 2552 2915 3035 1391 Size: 901 48 93 119 363 120 4072 Fragments arranged by size: 4072 901 363 120 119 93 48 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4745 BbvCI CC'TCA_GC Cuts at: 2634 2634 Size: 5716 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3907 3941 1903 Size: 34 1970 34 3678 Fragments arranged by size: 3678 1970 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3608 4818 3608 Size: 1210 4506 BclI T'GATC_A Cuts at: 1548 1548 Size: 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2026 4327 136 Size: 107 122 71 631 605 354 2301 1525 Fragments arranged by size: 2301 1525 631 605 354 122 107 71 BglII A'GATC_T Cuts at: 5711 5711 Size: 5716 BlpI GC'TnA_GC Cuts at: 3133 3133 Size: 5716 Bme1580I G_kGCm'C Cuts at: 1790 2383 2433 3777 4933 1790 Size: 593 50 1344 1156 2573 Fragments arranged by size: 2573 1344 1156 593 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5716 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2386 3447 4405 Size: 416 53 83 399 24 969 1061 958 Cuts at: 4405 442 Size: 1753 Fragments arranged by size: 1753 1061 969 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5716 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4376 1239 Size: 3137 2579 Bpu10I CC'TnA_GC Cuts at: 2634 2909 2634 Size: 275 5441 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3841 4706 5004 3841 Size: 865 298 4553 Fragments arranged by size: 4553 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4379 882 Size: 617 2880 2219 Fragments arranged by size: 2880 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2746 3507 493 Size: 711 1542 761 2702 Fragments arranged by size: 2702 1542 761 711 BsaBI GATnn'nnATC Cuts at: 5710 5710 Size: 5716 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2187 3523 3905 4540 Size: 53 83 186 936 654 1336 382 635 Cuts at: 4540 275 Size: 1451 Fragments arranged by size: 1451 1336 936 654 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4149 4547 4821 4968 5379 4149 Size: 398 274 147 411 4486 Fragments arranged by size: 4486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5491 Fragments arranged by size: 5491 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2300 2333 2753 1186 Size: 1114 33 420 4149 Fragments arranged by size: 4149 1114 420 33 BseYI C'CCAG_C Cuts at: 2159 2328 2793 4919 5415 2159 Size: 169 465 2126 496 2460 Fragments arranged by size: 2460 2126 496 465 169 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 1163 Size: 573 327 4816 Fragments arranged by size: 4816 573 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2030 2925 3166 3927 4955 1540 Size: 275 186 29 895 241 761 1028 2301 Fragments arranged by size: 2301 1028 895 761 275 241 186 29 BsiHKAI G_wGCw'C Cuts at: 2292 2745 2819 3777 3862 4933 2292 Size: 453 74 958 85 1071 3075 Fragments arranged by size: 3075 1071 958 453 85 74 BsmI GAATG_Cn' Cuts at: 3227 3320 3227 Size: 93 5623 BsmBI CGTCTCn'nnnn_ Cuts at: 2198 2198 Size: 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2292 2340 2383 2433 2552 2745 2819 Size: 399 502 48 43 50 119 193 74 Cuts at: 2819 2915 3035 3777 3862 4933 1391 Size: 96 120 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 502 399 193 120 119 96 85 74 50 48 43 BspEI T'CCGG_A Cuts at: 5379 5379 Size: 5716 BspHI T'CATG_A Cuts at: 3605 5335 3605 Size: 1730 3986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2085 2472 2505 2563 2841 3071 844 Size: 1241 387 33 58 278 230 3489 Fragments arranged by size: 3489 1241 387 278 230 58 33 BsrBI CCG'CTC Cuts at: 1394 1823 2535 2565 2898 3603 1394 Size: 429 712 30 333 705 3507 Fragments arranged by size: 3507 712 705 429 333 30 BsrDI GCAATG_nn' Cuts at: 65 1248 4211 4385 65 Size: 1183 2963 174 1396 Fragments arranged by size: 2963 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2295 2387 2586 4360 4547 1700 Size: 110 485 92 199 1774 187 2869 Fragments arranged by size: 2869 1774 485 199 187 110 92 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5716 BssHII G'CGCG_C Cuts at: 1925 2155 2965 2967 1925 Size: 230 810 2 4674 Fragments arranged by size: 4674 810 230 2 BssSI C'ACGA_G Cuts at: 3776 4788 3776 Size: 1012 4704 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5716 BstBI TT'CG_AA Cuts at: 4520 4520 Size: 5716 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5716 BstXI CCAn_nnnn'nTGG Cuts at: 2801 2801 Size: 5716 BstYI r'GATC_y Cuts at: 1972 2881 3046 3413 3798 3815 5267 5353 5365 Size: 909 165 367 385 17 1452 86 12 Cuts at: 5365 5711 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 909 385 367 346 165 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2435 2478 2435 Size: 43 5673 BtgI C'CryG_G Cuts at: 513 1065 2004 2098 2916 513 Size: 552 939 94 818 3313 Fragments arranged by size: 3313 939 818 552 94 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4977 Fragments arranged by size: 4977 683 56 BtsI GCAGTG_nn' Cuts at: 2872 3232 4026 4046 2872 Size: 360 794 20 4542 Fragments arranged by size: 4542 794 360 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5716 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5681 DraI TTT'AAA Cuts at: 3020 3020 Size: 5716 DraIII CAC_nnn'GTG Cuts at: 2218 2218 Size: 5716 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2171 4723 809 Size: 507 855 2552 1802 Fragments arranged by size: 2552 1802 855 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2027 2521 Size: 54 1425 50 275 18 177 20 494 Cuts at: 2521 2643 2922 3163 4052 5681 8 Size: 122 279 241 889 1629 43 Fragments arranged by size: 1629 1425 889 494 279 275 241 177 122 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 2027 2922 3163 1537 Size: 275 215 895 241 4090 Fragments arranged by size: 4090 895 275 241 215 EarI CTCTTCn'nnn_ Cuts at: 1353 3646 1353 Size: 2293 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2282 4303 4677 4823 387 Size: 1064 138 693 2021 374 146 1280 Fragments arranged by size: 2021 1280 1064 693 374 146 138 EcoICRI GAG'CTC Cuts at: 2290 2290 Size: 5716 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2989 3779 4376 5163 5431 1239 Size: 393 1357 790 597 787 268 1524 Fragments arranged by size: 1524 1357 790 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2174 2468 2174 Size: 294 5422 EcoO109I rG'GnC_Cy Cuts at: 2430 2474 2753 2430 Size: 44 279 5393 Fragments arranged by size: 5393 279 44 EcoRI G'AATT_C Cuts at: 3025 3403 3025 Size: 378 5338 FspI TGC'GCA Cuts at: 4222 4222 Size: 5716 HaeII r_GCGC'y Cuts at: 1678 4863 1678 Size: 3185 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2534 2566 4357 4389 4431 Size: 32 135 32 962 32 1791 32 42 Cuts at: 4431 4463 1373 Size: 32 2626 Fragments arranged by size: 2626 1791 962 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2182 2191 2959 3060 3306 669 Size: 988 281 244 9 768 101 246 3079 Fragments arranged by size: 3079 988 768 281 246 244 101 9 HindIII A'AGCT_T Cuts at: 748 748 Size: 5716 HpaI GTT'AAC Cuts at: 3306 3306 Size: 5716 KpnI G_GTAC'C Cuts at: 3041 3041 Size: 5716 MfeI C'AATT_G Cuts at: 3315 3315 Size: 5716 MluI A'CGCG_T Cuts at: 5592 5592 Size: 5716 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2868 4830 5014 1305 Size: 1563 1962 184 2007 Fragments arranged by size: 2007 1962 1563 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2645 10 Size: 54 1425 343 813 3081 Fragments arranged by size: 3081 1425 813 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2597 3674 4033 4192 518 Size: 648 1431 1077 359 159 2042 Fragments arranged by size: 2042 1431 1077 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2100 2305 3132 3809 4957 5202 1606 Size: 417 77 205 827 677 1148 245 2120 Fragments arranged by size: 2120 1148 827 677 417 245 205 77 NaeI GCC'GGC Cuts at: 2297 2297 Size: 5716 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4225 Fragments arranged by size: 4225 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5716 NgoMIV G'CCGG_C Cuts at: 2295 2295 Size: 5716 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5716 NotI GC'GGCC_GC Cuts at: 2027 3163 2027 Size: 1136 4580 NspI r_CATG'y Cuts at: 1132 1229 3074 4619 1132 Size: 97 1845 1545 2229 Fragments arranged by size: 2229 1845 1545 97 PasI CC'CwG_GG Cuts at: 1348 2358 1348 Size: 1010 4706 PciI A'CATG_T Cuts at: 4615 4615 Size: 5716 PflMI CCAn_nnn'nTGG Cuts at: 1108 2218 1108 Size: 1110 4606 PfoI T'CCnGG_A Cuts at: 2889 2889 Size: 5716 PmeI GTTT'AAAC Cuts at: 3020 3020 Size: 5716 PmlI CAC'GTG Cuts at: 2746 2746 Size: 5716 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3785 3817 5298 5330 3785 Size: 32 1481 32 4171 Fragments arranged by size: 4171 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2474 2753 2474 Size: 279 5437 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5716 PsiI TTA'TAA Cuts at: 3286 3286 Size: 5716 PspOMI G'GGCC_C Cuts at: 2429 2429 Size: 5716 PspXI vC'TCGA_Gb Cuts at: 2943 2943 Size: 5716 PstI C_TGCA'G Cuts at: 830 3068 830 Size: 2238 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5716 PvuII CAG'CTG Cuts at: 2305 2305 Size: 5716 SacI G_AGCT'C Cuts at: 2292 2292 Size: 5716 SacII CC_GC'GG Cuts at: 2101 2101 Size: 5716 SalI G'TCGA_C Cuts at: 1936 3058 1936 Size: 1122 4594 SbfI CC_TGCA'GG Cuts at: 3068 3068 Size: 5716 ScaI AGT'ACT Cuts at: 3964 5676 3964 Size: 1712 4004 SexAI A'CCwGG_T Cuts at: 2214 2214 Size: 5716 SfcI C'TryA_G Cuts at: 826 1045 3064 4199 4880 5071 826 Size: 219 2019 1135 681 191 1471 Fragments arranged by size: 2019 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5716 SgrAI Cr'CCGG_yG Cuts at: 1700 2295 1700 Size: 595 5121 SmaI CCC'GGG Cuts at: 1872 2547 3043 1872 Size: 675 496 4545 Fragments arranged by size: 4545 675 496 SmlI C'TyrA_G Cuts at: 820 1017 1943 2943 3820 4721 4983 820 Size: 197 926 1000 877 901 262 1553 Fragments arranged by size: 1553 1000 926 901 877 262 197 SnaBI TAC'GTA Cuts at: 493 3507 493 Size: 3014 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5716 SphI G_CATG'C Cuts at: 1132 3074 1132 Size: 1942 3774 SspI AAT'ATT Cuts at: 5 52 3640 5 Size: 47 3588 2081 Fragments arranged by size: 3588 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5716 StyI C'CwwG_G Cuts at: 513 1065 2004 2931 3155 513 Size: 552 939 927 224 3074 Fragments arranged by size: 3074 939 927 552 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2746 3743 3760 3913 4098 4543 325 Size: 1255 1166 997 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1166 997 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2526 3962 5674 Size: 274 80 33 51 1443 549 1436 1712 Cuts at: 5674 96 Size: 138 Fragments arranged by size: 1712 1443 1436 549 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5676 5708 5676 Size: 32 5684 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5716 XbaI T'CTAG_A Cuts at: 3052 3052 Size: 5716 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2801 2979 2801 Size: 178 5538 XhoI C'TCGA_G Cuts at: 1943 2943 1943 Size: 1000 4716 XmaI C'CCGG_G Cuts at: 1870 2545 3041 1870 Size: 675 496 4545 Fragments arranged by size: 4545 675 496 XmnI GAAnn'nnTTC Cuts at: 3845 3845 Size: 5716 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3524 276 Size: 53 83 186 936 1990 2468 Fragments arranged by size: 2468 1990 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI