Restriction map for
pFN21AE6495
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AvrII BamHI BanII BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaWI BseAI BseRI
BsiWI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstF5I
BstPI BstYI BstZ17I CelII ClaI
DpnII DraI DraII DrdI Eam1105I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FokI HinfI HpaI
HphI KpnI MaeI MamI MboI
MfeI MflI MroI MseI MunI
NcoI NdeI NdeII NheI NruI
NsiI NspV PacI PaeR7I PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI SacI
SalI Sau3AI SbfI ScaI SexAI
SfaNI SfuI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SspI StyI
SwaI TfiI Tru9I Tsp509I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
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AfaI 966 GTAC GT^AC
AspI 1201 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 708 CACGTG CAC^GTG
BsaI 124 GAGACC GGTCTC(1/5)
BsmBI 916 CGTCTC CGTCTC(1/5)
BsrDI 320 CATTGC GCAATG(2/0)
BssSI 715 CACGAG CACGAG(-5/-1)
BstAPI 186 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 976 CGGACCG CG^G[AT]CCG
DraIII 525 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
FseI 938 GGCCGGCC GGCCGG^CC
HindIII 628 AAGCTT A^AGCTT
NotI 334 GCGGCCGC GC^GGCCGC
PflFI 1201 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1029 CCACTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 708 CACGTG CAC^GTG
PmlI 708 CACGTG CAC^GTG
RsaI 966 GTAC GT^AC
RsrII 976 CGGACCG CG^G[AT]CCG
SfiI 603 GGCCGGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 116 GCATGC GCATG^C
StuI 99 AGGCCT AGG^CCT
Tsp45I 1351 GTGAC ^GT[GC]AC
Tth111I 1201 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1029 CCACTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 16 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG
187 CAGGTGCTG
AocI 427 CCTGAGG CC^T[ACGT]AGG
1320 CCTAAGG
AviII 316 TGCGCA TGC^GCA
1067 TGCGCA
BbeI 267 GGCGCC GGCGC^C
655 GGCGCC
BsaAI 442 TACGTG [CT]AC^GT[GA]
708 CACGTG
BsgI 1093 CTGCAC GTGCAG(16/14)
25 GTGCAG
BsrBI 108 CCGCTC CCGCTC(-3/-3)
374 CCGCTC
BstXI 903 CCAGGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
950 CCACCGCCGTGG
Bsu36I 427 CCTGAGG CC^T[ACGT]AGG
1320 CCTAAGG
Eco81I 427 CCTGAGG CC^T[ACGT]AGG
1320 CCTAAGG
EcoNI 21 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
537 CCTGGACGAGG
FspI 316 TGCGCA TGC^GCA
1067 TGCGCA
HincII 1159 GTCAAC GT[CT]^[GA]AC
1216 GTCAAC
HindII 1159 GTCAAC GT[CT]^[GA]AC
1216 GTCAAC
KasI 267 GGCGCC G^GCGCC
655 GGCGCC
KspI 842 CCGCGG CCGC^GG
1291 CCGCGG
MaeIII 533 GTTAC ^GT[ACGT]AC
1351 GTGAC
MluI 554 ACGCGT A^CGCGT
983 ACGCGT
MspA1I 842 CCGCGG C[AC]G^C[GT]G
1291 CCGCGG
NarI 267 GGCGCC GG^CGCC
655 GGCGCC
NspI 116 GCATGC [GA]CATG^[CT]
1223 GCATGT
PstI 184 CTGCAG CTGCA^G
235 CTGCAG
SacII 842 CCGCGG CCGC^GG
1291 CCGCGG
SapI 48 GCTCTTC GCTCTTC(1/4)
1176 GCTCTTC
SfcI 184 CTGCAG C^T[GA][CT]AG
235 CTGCAG
SfoI 267 GGCGCC GGC^GCC
655 GGCGCC
SgrAI 269 CGCCGGCG C[GA]^CCGG[CT]G
885 CGCCGGCG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
Aor51HI AspHI AvaII BalI BanI
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BsiEI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BspMI BsrFI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII ItaI Ksp632I MaeII
MboII MluNI MnlI MscI MslI
MspI MvaI MvnI MwoI NaeI
NciI NgoMIV NlaIII NlaIV Sau96I
ScrFI TaqI TseI TspRI TthHB8I
XcmI