(Linear) (Six-base) MAPSORT of: KIEE6495.seq Check: 1116 from: 1 to: 1374 KIEE6495.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 18 177 1374 Size: 18 159 1197 Fragments arranged by size: 1197 159 18 AfeI AGC'GCT Cuts at: 0 755 829 1175 1374 Size: 755 74 346 199 Fragments arranged by size: 755 346 199 74 AflIII A'CryG_T Cuts at: 0 359 554 983 1374 Size: 359 195 429 391 Fragments arranged by size: 429 391 359 195 AleI CACnn'nnGTG Cuts at: 0 955 1186 1374 Size: 955 231 188 Fragments arranged by size: 955 231 188 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 342 1374 Size: 342 1032 AlwNI CAG_nnn'CTG Cuts at: 0 21 192 1374 Size: 21 171 1182 Fragments arranged by size: 1182 171 21 BanI G'GyrC_C Cuts at: 0 267 655 1255 1374 Size: 267 388 600 119 Fragments arranged by size: 600 388 267 119 BbeI G_GCGC'C Cuts at: 0 271 659 1374 Size: 271 388 715 Fragments arranged by size: 715 388 271 BglI GCCn_nnn'nGGC Cuts at: 0 418 445 610 1374 Size: 418 27 165 764 Fragments arranged by size: 764 418 165 27 Bme1580I G_kGCm'C Cuts at: 0 896 1374 Size: 896 478 BmgBI CAC'GTC Cuts at: 0 305 360 1374 Size: 305 55 1014 Fragments arranged by size: 1014 305 55 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 534 1374 Size: 534 840 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 310 529 604 873 1374 Size: 247 63 219 75 269 501 Fragments arranged by size: 501 269 247 219 75 63 Bpu10I CC'TnA_GC Cuts at: 0 1270 1374 Size: 1270 104 BsaI GGTCTCn'nnnn_ Cuts at: 0 118 1374 Size: 118 1256 BsaAI yAC'GTr Cuts at: 0 444 710 1374 Size: 444 266 664 Fragments arranged by size: 664 444 266 BsaHI Gr'CG_yC Cuts at: 0 268 515 656 808 1011 1374 Size: 268 247 141 152 203 363 Fragments arranged by size: 363 268 247 203 152 141 BseYI C'CCAG_C Cuts at: 0 460 618 678 724 837 1374 Size: 460 158 60 46 113 537 Fragments arranged by size: 537 460 158 113 60 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 1078 1374 Size: 46 1032 296 Fragments arranged by size: 1032 296 46 BsiEI CG_ry'CG Cuts at: 0 63 204 208 338 413 476 774 884 Size: 63 141 4 130 75 63 298 110 Cuts at: 884 940 944 1187 1374 Size: 56 4 243 187 Fragments arranged by size: 298 243 187 141 130 110 75 63 63 56 4 4 BsiHKAI G_wGCw'C Cuts at: 0 83 296 484 1374 Size: 83 213 188 890 Fragments arranged by size: 890 213 188 83 BsmBI CGTCTCn'nnnn_ Cuts at: 0 922 1374 Size: 922 452 Bsp1286I G_dGCh'C Cuts at: 0 83 296 484 896 1374 Size: 83 213 188 412 478 Fragments arranged by size: 478 412 213 188 83 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 18 177 228 878 1374 Size: 18 159 51 650 496 Fragments arranged by size: 650 496 159 51 18 BsrBI CCG'CTC Cuts at: 0 110 376 1374 Size: 110 266 998 Fragments arranged by size: 998 266 110 BsrDI GCAATG_nn' Cuts at: 0 319 1374 Size: 319 1055 BsrFI r'CCGG_y Cuts at: 0 203 207 270 760 879 886 939 1282 Size: 203 4 63 490 119 7 53 343 Cuts at: 1282 1374 Size: 92 Fragments arranged by size: 490 343 203 119 92 63 53 7 4 BssSI C'ACGA_G Cuts at: 0 715 1374 Size: 715 659 BstAPI GCAn_nnn'nTGC Cuts at: 0 192 1374 Size: 192 1182 BstXI CCAn_nnnn'nTGG Cuts at: 0 910 957 1374 Size: 910 47 417 Fragments arranged by size: 910 417 47 Bsu36I CC'TnA_GG Cuts at: 0 428 1321 1374 Size: 428 893 53 Fragments arranged by size: 893 428 53 BtgI C'CryG_G Cuts at: 0 821 842 956 1124 1187 1291 1374 Size: 821 21 114 168 63 104 83 Fragments arranged by size: 821 168 114 104 83 63 21 BtsI GCAGTG_nn' Cuts at: 0 977 1029 1095 1374 Size: 977 52 66 279 Fragments arranged by size: 977 279 66 52 DraIII CAC_nnn'GTG Cuts at: 0 530 1374 Size: 530 844 EaeI y'GGCC_r Cuts at: 0 42 205 335 410 473 602 611 758 Size: 42 163 130 75 63 129 9 147 Cuts at: 758 771 863 881 912 937 941 998 1026 Size: 13 92 18 31 25 4 57 28 Cuts at: 1026 1121 1149 1184 1374 Size: 95 28 35 190 Fragments arranged by size: 190 163 147 130 129 95 92 75 63 57 42 35 31 28 28 25 18 13 9 4 EagI C'GGCC_G Cuts at: 0 205 335 410 473 771 881 937 941 Size: 205 130 75 63 298 110 56 4 Cuts at: 941 1184 1374 Size: 243 190 Fragments arranged by size: 298 243 205 190 130 110 75 63 56 4 EarI CTCTTCn'nnn_ Cuts at: 0 55 109 385 542 925 1183 1374 Size: 55 54 276 157 383 258 191 Fragments arranged by size: 383 276 258 191 157 55 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 235 1374 Size: 235 1139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 310 529 604 873 1374 Size: 247 63 219 75 269 501 Fragments arranged by size: 501 269 247 219 75 63 EcoNI CCTnn'n_nnAGG Cuts at: 0 25 541 1374 Size: 25 516 833 Fragments arranged by size: 833 516 25 FseI GG_CCGG'CC Cuts at: 0 943 1374 Size: 943 431 FspI TGC'GCA Cuts at: 0 318 1069 1374 Size: 318 751 305 Fragments arranged by size: 751 318 305 HaeII r_GCGC'y Cuts at: 0 214 271 277 424 659 757 831 1144 Size: 214 57 6 147 235 98 74 313 Cuts at: 1144 1177 1340 1374 Size: 33 163 34 Fragments arranged by size: 313 235 214 163 147 98 74 57 34 33 6 HincII GTy'rAC Cuts at: 0 1161 1218 1374 Size: 1161 57 156 Fragments arranged by size: 1161 156 57 HindIII A'AGCT_T Cuts at: 0 628 1374 Size: 628 746 KasI G'GCGC_C Cuts at: 0 267 655 1374 Size: 267 388 719 Fragments arranged by size: 719 388 267 MluI A'CGCG_T Cuts at: 0 554 983 1374 Size: 554 429 391 Fragments arranged by size: 554 429 391 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 287 1106 1374 Size: 287 819 268 Fragments arranged by size: 819 287 268 MscI TGG'CCA Cuts at: 0 613 1028 1123 1374 Size: 613 415 95 251 Fragments arranged by size: 613 415 251 95 MslI CAynn'nnrTG Cuts at: 0 324 955 1186 1374 Size: 324 631 231 188 Fragments arranged by size: 631 324 231 188 MspA1I CmG'CkG Cuts at: 0 844 1293 1374 Size: 844 449 81 Fragments arranged by size: 844 449 81 NaeI GCC'GGC Cuts at: 0 209 272 762 881 888 941 1284 1374 Size: 209 63 490 119 7 53 343 90 Fragments arranged by size: 490 343 209 119 90 63 53 7 NarI GG'CG_CC Cuts at: 0 268 656 1374 Size: 268 388 718 Fragments arranged by size: 718 388 268 NgoMIV G'CCGG_C Cuts at: 0 207 270 760 879 886 939 1282 1374 Size: 207 63 490 119 7 53 343 92 Fragments arranged by size: 490 343 207 119 92 63 53 7 NotI GC'GGCC_GC Cuts at: 0 335 1374 Size: 335 1039 NspI r_CATG'y Cuts at: 0 120 1227 1374 Size: 120 1107 147 Fragments arranged by size: 1107 147 120 PflMI CCAn_nnn'nTGG Cuts at: 0 1035 1374 Size: 1035 339 PfoI T'CCnGG_A Cuts at: 0 506 1374 Size: 506 868 PmlI CAC'GTG Cuts at: 0 710 1374 Size: 710 664 PstI C_TGCA'G Cuts at: 0 188 239 1374 Size: 188 51 1135 Fragments arranged by size: 1135 188 51 RsrII CG'GwC_CG Cuts at: 0 977 1374 Size: 977 397 SacII CC_GC'GG Cuts at: 0 845 1294 1374 Size: 845 449 80 Fragments arranged by size: 845 449 80 SapI GCTCTTCn'nnn_ Cuts at: 0 55 1183 1374 Size: 55 1128 191 Fragments arranged by size: 1128 191 55 SfcI C'TryA_G Cuts at: 0 184 235 1374 Size: 184 51 1139 Fragments arranged by size: 1139 184 51 SfiI GGCCn_nnn'nGGCC Cuts at: 0 610 1374 Size: 610 764 SfoI GGC'GCC Cuts at: 0 269 657 1374 Size: 269 388 717 Fragments arranged by size: 717 388 269 SgrAI Cr'CCGG_yG Cuts at: 0 270 886 1374 Size: 270 616 488 Fragments arranged by size: 616 488 270 SphI G_CATG'C Cuts at: 0 120 1374 Size: 120 1254 StuI AGG'CCT Cuts at: 0 101 1374 Size: 101 1273 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 24 994 1374 Size: 24 970 380 Fragments arranged by size: 970 380 24 Tth111I GACn'n_nGTC Cuts at: 0 1204 1374 Size: 1204 170 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 597 666 819 957 1374 Size: 597 69 153 138 417 Fragments arranged by size: 597 417 153 138 69 Enzymes that do cut: AarI AfeI AflIII AleI AlfI AlwNI BanI BbeI BglI Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI FseI FspI HaeII HincII HindIII KasI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI PflMI PfoI PmlI PstI RsrII SacII SapI SfcI SfiI SfoI SgrAI SphI StuI TaqII Tth111I XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmrI BmtI BpuEI BsaBI BsaWI BsaXI BseRI BsiWI BsmI BspEI BspHI BsrGI BssHII BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI EcoICRI EcoO109I EcoRI EcoRV FalI FspAI Hin4I HpaI KpnI MfeI NcoI NdeI NheI NruI NsiI PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SgfI SmaI SmlI SnaBI SpeI SrfI SspI StyI SwaI TatI TstI XbaI XhoI XmaI XmnI ZraI