################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FbaI FseI FspI HindIII KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcyI 46 GGCGCC G[GA]^CG[CT]C Asp700I 571 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 450 CCCGAG C^[CT]CG[GA]G BbeI 46 GGCGCC GGCGC^C BcnI 111 CCGGG CC^[GC]GG BsaHI 46 GGCGCC G[GA]^CG[CT]C BsoBI 450 CCCGAG C^[CT]CG[GA]G BsrDI 683 GCAATG GCAATG(2/0) BstYI 340 GGATCT [GA]^GATC[CT] DraII 27 GGGTCCT [GA]G^G[ACGT]CC[CT] DrdI 115 GACTCCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 27 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoT22I 1158 ATGCAT ATGCA^T HgaI 825 GACGC GACGC(5/10) Hin1I 46 GGCGCC G[GA]^CG[CT]C HincII 871 GTTAAC GT[CT]^[GA]AC HindII 871 GTTAAC GT[CT]^[GA]AC HpaI 871 GTTAAC GTT^AAC HphI 268 TCACC GGTGA(8/7) KasI 46 GGCGCC G^GCGCC MflI 340 GGATCT [GA]^GATC[CT] MspA1I 34 CCGCTG C[AC]G^C[GT]G NarI 46 GGCGCC GG^CGCC NciI 111 CCGGG CC^[GC]GG NsiI 1158 ATGCAT ATGCA^T NspI 641 ACATGC [GA]CATG^[CT] PpuMI 27 GGGTCCT [GA]G^G[AT]CC[CT] SapI 150 GCTCTTC GCTCTTC(1/4) SfaNI 1002 GATGC GCATC(5/9) SfoI 46 GGCGCC GGC^GCC SmlI 1006 CTCAAG C^T[CT][GA]AG SspI 780 AATATT AAT^ATT XcmI 845 CCATTAAACTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 340 GGATCT [GA]^GATC[CT] XmnI 571 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Acc65I 739 GGTACC G^GTACC 1058 GGTACC AccII 127 CGCG CG^CG 178 CGCG AfaI 740 GTAC GT^AC 1059 GTAC Asp718I 739 GGTACC G^GTACC 1058 GGTACC AvaII 28 GGTCC G^G[AT]CC 165 GGACC BglI 135 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 175 GCCCGCGAGGC BsgI 196 CTGCAC GTGCAG(16/14) 301 GTGCAG BsmAI 497 GAGAC GTCTC(1/5) 306 GTCTC BstUI 127 CGCG CG^CG 178 CGCG EaeI 328 TGGCCG [CT]^GGCC[GA] 378 TGGCCG EarI 151 CTCTTC CTCTTC(1/4) 223 GAAGAG HapII 111 CCGG C^CGG 331 CCGG HpaII 111 CCGG C^CGG 331 CCGG KpnI 739 GGTACC GGTAC^C 1058 GGTACC Ksp632I 151 CTCTTC CTCTTC(1/4) 223 GAAGAG MspI 111 CCGG C^CGG 331 CCGG MvnI 127 CGCG CG^CG 178 CGCG NlaIII 43 CATG CATG^ 642 CATG RsaI 740 GTAC GT^AC 1059 GTAC TaqI 233 TCGA T^CGA 993 TCGA Tsp45I 250 GTCAC ^GT[GC]AC 948 GTCAC TthHB8I 233 TCGA T^CGA 993 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI AspHI BanI BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HinfI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIV PleI Sau3AI Sau96I ScrFI Tru9I TseI Tsp509I TspRI