(Circular) (Six-base) MAPSORT of: pFN21AE2227.seq Check: 6034 from: 1 to: 5872 pFN21AE2227 5872 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3215 1937 Size: 1278 4594 Acc65I G'GTAC_C Cuts at: 2744 3063 3193 2744 Size: 319 130 5423 Fragments arranged by size: 5423 319 130 AclI AA'CG_TT Cuts at: 1563 3999 4372 1563 Size: 2436 373 3063 Fragments arranged by size: 3063 2436 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2760 3935 5319 5587 1632 Size: 1128 1175 1384 268 1917 Fragments arranged by size: 1917 1384 1175 1128 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5675 AflIII A'CryG_T Cuts at: 1203 4771 5748 1203 Size: 3568 977 1327 Fragments arranged by size: 3568 1327 977 AgeI A'CCGG_T Cuts at: 4703 4703 Size: 5872 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5872 AlwNI CAG_nnn'CTG Cuts at: 2477 2513 3032 3615 5187 2477 Size: 36 519 583 1572 3162 Fragments arranged by size: 3162 1572 583 519 36 ApaLI G'TGCA_C Cuts at: 3929 5085 3929 Size: 1156 4716 ApoI r'AATT_y Cuts at: 1493 2414 2668 3073 3181 3407 3559 5668 1493 Size: 921 254 405 108 226 152 2109 1697 Fragments arranged by size: 2109 1697 921 405 254 226 152 108 AseI AT'TA_AT Cuts at: 160 1032 4426 160 Size: 872 3394 1606 Fragments arranged by size: 3394 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 2455 3197 1870 Size: 73 512 742 4545 Fragments arranged by size: 4545 742 512 73 BamHI G'GATC_C Cuts at: 3202 3569 3202 Size: 367 5505 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2639 2744 3063 3193 Size: 325 217 627 264 588 105 319 130 Cuts at: 3193 4648 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 588 325 319 264 217 130 105 BanII G_rGCy'C Cuts at: 1391 3191 1391 Size: 1800 4072 BbeI G_GCGC'C Cuts at: 2055 2055 Size: 5872 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4901 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4063 4097 1903 Size: 34 2126 34 3678 Fragments arranged by size: 3678 2126 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3764 4974 3764 Size: 1210 4662 BclI T'GATC_A Cuts at: 1548 1548 Size: 5872 BfrBI ATG'CAT Cuts at: 3165 3165 Size: 5872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2146 2186 4483 Size: 107 122 71 631 605 474 40 2297 Cuts at: 4483 136 Size: 1525 Fragments arranged by size: 2297 1525 631 605 474 122 107 71 40 BglII A'GATC_T Cuts at: 5867 5867 Size: 5872 BlpI GC'TnA_GC Cuts at: 3289 3289 Size: 5872 Bme1580I G_kGCm'C Cuts at: 1790 3933 5089 1790 Size: 2143 1156 2573 Fragments arranged by size: 2573 2143 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5872 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2071 2439 2645 Size: 416 53 83 399 24 654 368 206 Cuts at: 2645 3603 4561 442 Size: 958 958 1753 Fragments arranged by size: 1753 958 958 654 416 399 368 206 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5872 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2107 2267 2370 2535 4532 1239 Size: 868 160 103 165 1997 2579 Fragments arranged by size: 2579 1997 868 165 160 103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2996 3997 4862 5160 2996 Size: 1001 865 298 3708 Fragments arranged by size: 3708 1001 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4535 882 Size: 617 3036 2219 Fragments arranged by size: 3036 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3663 493 Size: 711 2459 2702 Fragments arranged by size: 2702 2459 711 BsaBI GATnn'nnATC Cuts at: 5866 5866 Size: 5872 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 3679 4061 4696 Size: 53 83 186 936 519 1627 382 635 Cuts at: 4696 275 Size: 1451 Fragments arranged by size: 1627 1451 936 635 519 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4305 4703 4977 5124 5535 4305 Size: 398 274 147 411 4642 Fragments arranged by size: 4642 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2056 2086 2493 2523 1178 Size: 30 165 30 653 30 407 30 4527 Fragments arranged by size: 4527 653 407 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5872 BseYI C'CCAG_C Cuts at: 2103 2470 5075 5571 2103 Size: 367 2605 496 2404 Fragments arranged by size: 2605 2404 496 367 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2327 1163 Size: 573 450 141 4708 Fragments arranged by size: 4708 573 450 141 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3322 4083 5111 1540 Size: 275 186 1321 761 1028 2301 Fragments arranged by size: 2301 1321 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2089 2157 2603 3933 4018 5089 2089 Size: 68 446 1330 85 1071 2872 Fragments arranged by size: 2872 1330 1071 446 85 68 BsmI GAATG_Cn' Cuts at: 3383 3476 3383 Size: 93 5779 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 2157 2603 3191 3933 4018 5089 Size: 399 299 68 446 588 742 85 1071 Cuts at: 5089 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 588 446 399 299 85 68 BspEI T'CCGG_A Cuts at: 5535 5535 Size: 5872 BspHI T'CATG_A Cuts at: 3761 5491 3761 Size: 1730 4142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3227 844 Size: 2383 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3759 1394 Size: 429 1936 3507 Fragments arranged by size: 3507 1936 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2695 4367 4541 65 Size: 1183 1447 1672 174 1396 Fragments arranged by size: 1672 1447 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4516 4703 1700 Size: 110 2706 187 2869 Fragments arranged by size: 2869 2706 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5872 BssSI C'ACGA_G Cuts at: 3932 4944 3932 Size: 1012 4860 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5872 BstBI TT'CG_AA Cuts at: 4676 4676 Size: 5872 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5872 BstYI r'GATC_y Cuts at: 1972 2345 3202 3569 3954 3971 5423 5509 5521 Size: 373 857 367 385 17 1452 86 12 Cuts at: 5521 5867 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 857 385 373 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5320 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5133 Fragments arranged by size: 5133 683 56 BtsI GCAGTG_nn' Cuts at: 2601 2628 3388 4182 4202 2601 Size: 27 760 794 20 4271 Fragments arranged by size: 4271 794 760 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5872 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5837 DraI TTT'AAA Cuts at: 3176 3176 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2126 4879 809 Size: 507 810 2753 1802 Fragments arranged by size: 2753 1802 810 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2333 2383 3319 Size: 54 1425 50 275 18 503 50 936 Cuts at: 3319 4208 5837 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 936 889 503 275 54 50 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3319 1537 Size: 275 1507 4090 Fragments arranged by size: 4090 1507 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2162 2223 3802 1353 Size: 809 61 1579 3423 Fragments arranged by size: 3423 1579 809 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4459 4833 4979 387 Size: 1064 138 2870 374 146 1280 Fragments arranged by size: 2870 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2107 2267 2370 2535 2760 3935 4532 Size: 393 475 160 103 165 225 1175 597 Cuts at: 4532 5319 5587 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1175 787 597 475 393 268 225 165 160 103 EcoO109I rG'GnC_Cy Cuts at: 2033 2033 Size: 5872 EcoRI G'AATT_C Cuts at: 3181 3559 3181 Size: 378 5494 FspI TGC'GCA Cuts at: 4378 4378 Size: 5872 HaeII r_GCGC'y Cuts at: 1678 2026 2055 2192 2201 5019 1678 Size: 348 29 137 9 2818 2531 Fragments arranged by size: 2818 2531 348 137 29 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2056 2088 2330 2362 4513 Size: 32 135 32 484 32 242 32 2151 Cuts at: 4513 4545 4587 4619 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2151 484 242 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2878 3216 3462 669 Size: 988 281 940 338 246 3079 Fragments arranged by size: 3079 988 940 338 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5872 HpaI GTT'AAC Cuts at: 2878 3462 2878 Size: 584 5288 KasI G'GCGC_C Cuts at: 2051 2051 Size: 5872 KpnI G_GTAC'C Cuts at: 2748 3067 3197 2748 Size: 319 130 5423 Fragments arranged by size: 5423 319 130 MfeI C'AATT_G Cuts at: 3471 3471 Size: 5872 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2344 2966 3146 4986 5170 1305 Size: 1039 622 180 1840 184 2007 Fragments arranged by size: 2007 1840 1039 622 184 180 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4050 Fragments arranged by size: 4050 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3830 4189 4348 518 Size: 648 843 1821 359 159 2042 Fragments arranged by size: 2042 1821 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 3288 3965 5113 5358 1606 Size: 435 1247 677 1148 245 2120 Fragments arranged by size: 2120 1247 1148 677 435 245 NarI GG'CG_CC Cuts at: 2052 2052 Size: 5872 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5320 NdeI CA'TA_TG Cuts at: 387 387 Size: 5872 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5872 NotI GC'GGCC_GC Cuts at: 3319 3319 Size: 5872 NsiI A_TGCA'T Cuts at: 3167 3167 Size: 5872 NspI r_CATG'y Cuts at: 1132 1229 2650 3230 4775 1132 Size: 97 1421 580 1545 2229 Fragments arranged by size: 2229 1545 1421 580 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5872 PciI A'CATG_T Cuts at: 4771 4771 Size: 5872 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5872 PfoI T'CCnGG_A Cuts at: 2926 2926 Size: 5872 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 5454 5486 3941 Size: 32 1481 32 4327 Fragments arranged by size: 4327 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2033 2033 Size: 5872 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5872 PsiI TTA'TAA Cuts at: 3442 3442 Size: 5872 PstI C_TGCA'G Cuts at: 830 3224 830 Size: 2394 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 SalI G'TCGA_C Cuts at: 1936 3214 1936 Size: 1278 4594 SapI GCTCTTCn'nnn_ Cuts at: 2162 2162 Size: 5872 SbfI CC_TGCA'GG Cuts at: 3224 3224 Size: 5872 ScaI AGT'ACT Cuts at: 4120 5832 4120 Size: 1712 4160 SfcI C'TryA_G Cuts at: 826 1045 3220 4355 5036 5227 826 Size: 219 2175 1135 681 191 1471 Fragments arranged by size: 2175 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2053 2053 Size: 5872 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5872 SmaI CCC'GGG Cuts at: 1872 3199 1872 Size: 1327 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3011 3976 4877 5139 820 Size: 197 926 1068 965 901 262 1553 Fragments arranged by size: 1553 1068 965 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3663 493 Size: 3170 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 3230 1132 Size: 2098 3774 SspI AAT'ATT Cuts at: 5 52 2787 3796 5 Size: 47 2735 1009 2081 Fragments arranged by size: 2735 2081 1009 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5872 StyI C'CwwG_G Cuts at: 513 1065 3311 513 Size: 552 2246 3074 Fragments arranged by size: 3074 2246 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2608 2627 3899 3916 4069 4254 4699 Size: 1255 1028 19 1272 17 153 185 445 Cuts at: 4699 325 Size: 1498 Fragments arranged by size: 1498 1272 1255 1028 445 185 153 19 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4118 5830 96 Size: 274 80 33 51 1443 2141 1712 138 Fragments arranged by size: 2141 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2274 2306 5832 5864 2274 Size: 32 3526 32 2282 Fragments arranged by size: 3526 2282 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5872 XbaI T'CTAG_A Cuts at: 3208 3208 Size: 5872 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2857 2011 Size: 846 5026 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5872 XmaI C'CCGG_G Cuts at: 1870 3197 1870 Size: 1327 4545 XmnI GAAnn'nnTTC Cuts at: 2580 4001 2580 Size: 1421 4451 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI