(Linear) (Six-base) MAPSORT of: KIEE2227.seq Check: 4326 from: 1 to: 1167 KIEE2227.orf With 182 enzymes: SgfI * July 15, 2010 18:56 .. Acc65I G'GTAC_C Cuts at: 0 739 1058 1167 Size: 739 319 109 Fragments arranged by size: 739 319 109 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 755 1167 Size: 755 412 AlwNI CAG_nnn'CTG Cuts at: 0 472 508 1027 1167 Size: 472 36 519 140 Fragments arranged by size: 519 472 140 36 ApoI r'AATT_y Cuts at: 0 409 663 1068 1167 Size: 409 254 405 99 Fragments arranged by size: 409 405 254 99 AvaI C'yCGr_G Cuts at: 0 450 1167 Size: 450 717 BanI G'GyrC_C Cuts at: 0 46 634 739 1058 1167 Size: 46 588 105 319 109 Fragments arranged by size: 588 319 109 105 46 BbeI G_GCGC'C Cuts at: 0 50 1167 Size: 50 1117 BfrBI ATG'CAT Cuts at: 0 1160 1167 Size: 1160 7 BglI GCCn_nnn'nGGC Cuts at: 0 141 181 1167 Size: 141 40 986 Fragments arranged by size: 986 141 40 BmrI ACTGGGnnnn_n' Cuts at: 0 66 434 640 1167 Size: 66 368 206 527 Fragments arranged by size: 527 368 206 66 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 102 262 365 530 1167 Size: 102 160 103 165 637 Fragments arranged by size: 637 165 160 103 102 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 991 1167 Size: 991 176 BsaHI Gr'CG_yC Cuts at: 0 47 1167 Size: 47 1120 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 51 81 488 518 1167 Size: 51 30 407 30 649 Fragments arranged by size: 649 407 51 30 30 BseYI C'CCAG_C Cuts at: 0 98 465 1167 Size: 98 367 702 Fragments arranged by size: 702 367 98 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 322 1167 Size: 181 141 845 Fragments arranged by size: 845 181 141 BsiHKAI G_wGCw'C Cuts at: 0 84 152 598 1167 Size: 84 68 446 569 Fragments arranged by size: 569 446 84 68 Bsp1286I G_dGCh'C Cuts at: 0 84 152 598 1167 Size: 84 68 446 569 Fragments arranged by size: 569 446 84 68 BsrDI GCAATG_nn' Cuts at: 0 690 1167 Size: 690 477 BstYI r'GATC_y Cuts at: 0 340 1167 Size: 340 827 BtsI GCAGTG_nn' Cuts at: 0 596 623 1167 Size: 596 27 544 Fragments arranged by size: 596 544 27 DrdI GACnn_nn'nnGTC Cuts at: 0 121 1167 Size: 121 1046 EaeI y'GGCC_r Cuts at: 0 328 378 1167 Size: 328 50 789 Fragments arranged by size: 789 328 50 EarI CTCTTCn'nnn_ Cuts at: 0 157 218 1167 Size: 157 61 949 Fragments arranged by size: 949 157 61 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 102 262 365 530 755 1167 Size: 102 160 103 165 225 412 Fragments arranged by size: 412 225 165 160 103 102 EcoO109I rG'GnC_Cy Cuts at: 0 28 1167 Size: 28 1139 HaeII r_GCGC'y Cuts at: 0 21 50 187 196 1167 Size: 21 29 137 9 971 Fragments arranged by size: 971 137 29 21 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 51 83 325 357 1167 Size: 51 32 242 32 810 Fragments arranged by size: 810 242 51 32 32 HincII GTy'rAC Cuts at: 0 873 1167 Size: 873 294 HpaI GTT'AAC Cuts at: 0 873 1167 Size: 873 294 KasI G'GCGC_C Cuts at: 0 46 1167 Size: 46 1121 KpnI G_GTAC'C Cuts at: 0 743 1062 1167 Size: 743 319 105 Fragments arranged by size: 743 319 105 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 339 961 1141 1167 Size: 339 622 180 26 Fragments arranged by size: 622 339 180 26 MspA1I CmG'CkG Cuts at: 0 36 1167 Size: 36 1131 NarI GG'CG_CC Cuts at: 0 47 1167 Size: 47 1120 NsiI A_TGCA'T Cuts at: 0 1162 1167 Size: 1162 5 NspI r_CATG'y Cuts at: 0 645 1167 Size: 645 522 PfoI T'CCnGG_A Cuts at: 0 921 1167 Size: 921 246 PpuMI rG'GwC_Cy Cuts at: 0 28 1167 Size: 28 1139 SapI GCTCTTCn'nnn_ Cuts at: 0 157 1167 Size: 157 1010 SfoI GGC'GCC Cuts at: 0 48 1167 Size: 48 1119 SmlI C'TyrA_G Cuts at: 0 1006 1167 Size: 1006 161 SspI AAT'ATT Cuts at: 0 782 1167 Size: 782 385 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 603 622 1167 Size: 603 19 545 Fragments arranged by size: 603 545 19 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 269 301 1167 Size: 269 32 866 Fragments arranged by size: 866 269 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 852 1167 Size: 852 315 XmnI GAAnn'nnTTC Cuts at: 0 575 1167 Size: 575 592 Enzymes that do cut: Acc65I AcuI AlwNI ApoI AvaI BanI BbeI BfrBI BglI BmrI BpmI BpuEI BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BsrDI BstYI BtsI DrdI EaeI EarI Eco57MI EcoO109I HaeII Hin4I HincII HpaI KasI KpnI MmeI MspA1I NarI NsiI NspI PfoI PpuMI SapI SfoI SmlI SspI TaqII TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII MfeI MluI MscI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI StyI SwaI TatI Tth111I XbaI XhoI XmaI ZraI