Restriction map for
pFN21AE6587
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BsrI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HgaI
HincII HindII HindIII HinfI HpaI
HphI KpnI Ksp632I KspI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MroI MscI MseI MslI
MunI MvnI NcoI NdeI NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 185 GGCGCC G[GA]^CG[CT]C
AfaI 135 GTAC GT^AC
AlwI 430 GATCC GGATC(4/5)
AspHI 264 GAGCTC G[AT]GC[AT]^C
AvaI 215 CCCGGG C^[CT]CG[GA]G
BanI 185 GGCGCC G^G[CT][GA]CC
BbeI 185 GGCGCC GGCGC^C
BfaI 509 CTAG C^TAG
BglI 66 GCCACGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 286 CTGGAG CTGGAG(16/14)
BsaHI 185 GGCGCC G[GA]^CG[CT]C
BsaI 4 GAGACC GGTCTC(1/5)
BseRI 130 GAGGAG GAGGAG(10/8)
BsiHKAI 264 GAGCTC G[AT]GC[AT]^C
BsmAI 4 GAGAC GTCTC(1/5)
BsoBI 215 CCCGGG C^[CT]CG[GA]G
BsrFI 182 GCCGGC [GA]^CCGG[CT]
BssSI 127 CACGAG CACGAG(-5/-1)
Cfr10I 182 GCCGGC [GA]^CCGG[CT]
DpnII 430 GATC ^GATC
DraII 377 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 67 CCACGG C^C[GA][CT]GG
EcoO109I 377 AGGGCCT [GA]G^G[ACGT]CC[CT]
Hin1I 185 GGCGCC G[GA]^CG[CT]C
KasI 185 GGCGCC G^GCGCC
MaeI 509 CTAG C^TAG
MboI 430 GATC ^GATC
MboII 41 TCTTC GAAGA(8/7)
MspA1I 49 CAGCTG C[AC]G^C[GT]G
NaeI 182 GCCGGC GCC^GGC
NarI 185 GGCGCC GG^CGCC
NdeII 430 GATC ^GATC
NgoMIV 182 GCCGGC G^CCGGC
PstI 484 CTGCAG CTGCA^G
PvuII 49 CAGCTG CAG^CTG
RsaI 135 GTAC GT^AC
SacI 264 GAGCTC GAGCT^C
Sau3AI 430 GATC ^GATC
SfaNI 489 GATGC GCATC(5/9)
SfcI 484 CTGCAG C^T[GA][CT]AG
SfoI 185 GGCGCC GGC^GCC
SmaI 215 CCCGGG CCC^GGG
TaqI 349 TCGA T^CGA
TthHB8I 349 TCGA T^CGA
XmaCI 215 CCCGGG C^CCGGG
XmaI 215 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 123 GGACC G^G[AT]CC
453 GGACC
BlpI 51 GCTGAGC GC^T[ACGT]AGC
96 GCTTAGC
Bpu1102I 51 GCTGAGC GC^T[ACGT]AGC
96 GCTTAGC
BsiYI 193 CCACTGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
327 CCTGCCATCGG
BslI 193 CCACTGTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
327 CCTGCCATCGG
BsmFI 122 GGGAC GGGAC(10/14)
203 GGGAC
BspMI 297 ACCTGC ACCTGC(4/8)
115 GCAGGT
BstF5I 153 CATCC GGATG(2/0)
282 CATCC
BstXI 221 CCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
278 CCAACATCCTGG
CelII 51 GCTGAGC GC^T[ACGT]AGC
96 GCTTAGC
CfoI 186 GCGC GCG^C
337 GCGC
EaeI 64 CGGCCA [CT]^GGCC[GA]
180 TGGCCG
FokI 153 CATCC GGATG(9/13)
282 CATCC
HaeII 185 GGCGCC [GA]GCGC^[CT]
336 GGCGCT
HhaI 186 GCGC GCG^C
337 GCGC
HinP1I 186 GCGC G^CGC
337 GCGC
SmlI 267 CTCAAG C^T[CT][GA]AG
472 CTTGAG
TspRI 194 CACTG CA[GC]TG[ACGT][ACGT]^
426 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI EcoRII Fnu4HI
HaeIII HapII HpaII ItaI MnlI
MspI MvaI MwoI NciI NlaIV
Sau96I ScrFI TseI