(Circular) (Six-base) MAPSORT of: pFN21AE6587.seq Check: 946 from: 1 to: 5230 pFN21AE6587 5230 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2111 2311 2111 Size: 200 5030 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3040 278 Size: 53 83 186 936 1504 2468 Fragments arranged by size: 2468 1504 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2573 1937 Size: 636 4594 Acc65I G'GTAC_C Cuts at: 2551 2551 Size: 5230 AclI AA'CG_TT Cuts at: 1563 3357 3730 1563 Size: 1794 373 3063 Fragments arranged by size: 3063 1794 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3293 4677 4945 1632 Size: 1661 1384 268 1917 Fragments arranged by size: 1917 1661 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5033 AflIII A'CryG_T Cuts at: 1203 4129 5106 1203 Size: 2926 977 1327 Fragments arranged by size: 2926 1327 977 AgeI A'CCGG_T Cuts at: 4061 4061 Size: 5230 AhdI GACnn_n'nnGTC Cuts at: 3959 3959 Size: 5230 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5230 AlwNI CAG_nnn'CTG Cuts at: 2973 4545 2973 Size: 1572 3658 ApaLI G'TGCA_C Cuts at: 3287 4443 3287 Size: 1156 4074 ApoI r'AATT_y Cuts at: 1493 2539 2765 2917 5026 1493 Size: 1046 226 152 2109 1697 Fragments arranged by size: 2109 1697 1046 226 152 AseI AT'TA_AT Cuts at: 160 1032 3784 160 Size: 872 2752 1606 Fragments arranged by size: 2752 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5230 AvaI C'yCGr_G Cuts at: 1870 1943 2220 2555 1870 Size: 73 277 335 4545 Fragments arranged by size: 4545 335 277 73 BamHI G'GATC_C Cuts at: 2560 2927 2560 Size: 367 4863 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2190 2551 4006 618 Size: 325 217 627 403 361 1455 1842 Fragments arranged by size: 1842 1455 627 403 361 325 217 BanII G_rGCy'C Cuts at: 1391 2273 2298 2523 2549 1391 Size: 882 25 225 26 4072 Fragments arranged by size: 4072 882 225 26 25 BbeI G_GCGC'C Cuts at: 2194 2194 Size: 5230 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4259 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3421 3455 1903 Size: 34 1484 34 3678 Fragments arranged by size: 3678 1484 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3122 4332 3122 Size: 1210 4020 BclI T'GATC_A Cuts at: 1548 1548 Size: 5230 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2077 3841 136 Size: 107 122 71 631 605 405 1764 1525 Fragments arranged by size: 1764 1525 631 605 405 122 107 71 BglII A'GATC_T Cuts at: 5225 5225 Size: 5230 BlpI GC'TnA_GC Cuts at: 2057 2102 2647 2057 Size: 45 545 4640 Fragments arranged by size: 4640 545 45 Bme1580I G_kGCm'C Cuts at: 1790 2362 3291 4447 1790 Size: 572 929 1156 2573 Fragments arranged by size: 2573 1156 929 572 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5230 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2961 3919 442 Size: 416 53 83 399 24 1544 958 1753 Fragments arranged by size: 1753 1544 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5230 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 3890 1239 Size: 1073 1578 2579 Fragments arranged by size: 2579 1578 1073 Bpu10I CC'TnA_GC Cuts at: 2498 2498 Size: 5230 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2257 2498 3355 4220 4518 2257 Size: 241 857 865 298 2969 Fragments arranged by size: 2969 865 857 298 241 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 3893 882 Size: 617 504 1890 2219 Fragments arranged by size: 2219 1890 617 504 BsaAI yAC'GTr Cuts at: 493 1204 3021 493 Size: 711 1817 2702 Fragments arranged by size: 2702 1817 711 BsaBI GATnn'nnATC Cuts at: 5224 5224 Size: 5230 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2191 3037 3419 4054 Size: 53 83 186 936 658 846 382 635 Cuts at: 4054 275 Size: 1451 Fragments arranged by size: 1451 936 846 658 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3663 4061 4335 4482 4893 3663 Size: 398 274 147 411 4000 Fragments arranged by size: 4000 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2064 2094 1178 Size: 30 165 30 661 30 4314 Fragments arranged by size: 4314 661 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2150 1186 Size: 964 4266 BseYI C'CCAG_C Cuts at: 2522 4433 4929 2522 Size: 1911 496 2823 Fragments arranged by size: 2823 1911 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4657 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2680 3441 4469 1540 Size: 275 186 679 761 1028 2301 Fragments arranged by size: 2301 1028 761 679 275 186 BsiHKAI G_wGCw'C Cuts at: 2273 3291 3376 4447 2273 Size: 1018 85 1071 3056 Fragments arranged by size: 3056 1071 1018 85 BsmI GAATG_Cn' Cuts at: 2741 2834 2741 Size: 93 5137 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2273 2298 2362 2523 2549 3291 3376 Size: 399 483 25 64 161 26 742 85 Cuts at: 3376 4447 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 483 399 161 85 64 26 25 BspEI T'CCGG_A Cuts at: 4893 4893 Size: 5230 BspHI T'CATG_A Cuts at: 3119 4849 3119 Size: 1730 3500 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2111 2311 2585 844 Size: 1267 200 274 3489 Fragments arranged by size: 3489 1267 274 200 BsrBI CCG'CTC Cuts at: 1394 1823 3117 1394 Size: 429 1294 3507 Fragments arranged by size: 3507 1294 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3725 3899 65 Size: 1183 2477 174 1396 Fragments arranged by size: 2477 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2187 3874 4061 1700 Size: 110 377 1687 187 2869 Fragments arranged by size: 2869 1687 377 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5230 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5230 BssSI C'ACGA_G Cuts at: 2132 3290 4302 2132 Size: 1158 1012 3060 Fragments arranged by size: 3060 1158 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5230 BstBI TT'CG_AA Cuts at: 4034 4034 Size: 5230 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5230 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2290 2233 Size: 57 5173 BstYI r'GATC_y Cuts at: 1972 2560 2927 3312 3329 4781 4867 4879 5225 Size: 588 367 385 17 1452 86 12 346 Cuts at: 5225 1972 Size: 1977 Fragments arranged by size: 1977 1452 588 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2072 513 Size: 552 939 68 3671 Fragments arranged by size: 3671 939 552 68 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4491 Fragments arranged by size: 4491 683 56 BtsI GCAGTG_nn' Cuts at: 2746 3540 3560 2746 Size: 794 20 4416 Fragments arranged by size: 4416 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5230 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5195 DraI TTT'AAA Cuts at: 2534 2534 Size: 5230 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4237 809 Size: 507 2921 1802 Fragments arranged by size: 2921 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2069 2185 2677 Size: 54 1425 50 275 18 239 116 492 Cuts at: 2677 3566 5195 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 492 275 239 116 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2677 1537 Size: 275 865 4090 Fragments arranged by size: 4090 865 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3160 1353 Size: 1807 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2032 3817 4191 4337 387 Size: 1064 138 443 1785 374 146 1280 Fragments arranged by size: 1785 1280 1064 443 374 146 138 EcoICRI GAG'CTC Cuts at: 2271 2271 Size: 5230 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2312 3293 3890 4677 4945 1239 Size: 393 680 981 597 787 268 1524 Fragments arranged by size: 1524 981 787 680 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2383 2383 Size: 5230 EcoRI G'AATT_C Cuts at: 2539 2917 2539 Size: 378 4852 FspI TGC'GCA Cuts at: 3736 3736 Size: 5230 HaeII r_GCGC'y Cuts at: 1678 2194 2345 4377 1678 Size: 516 151 2032 2531 Fragments arranged by size: 2531 2032 516 151 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2142 2174 3871 3903 3945 Size: 32 135 32 570 32 1697 32 42 Cuts at: 3945 3977 1373 Size: 32 2626 Fragments arranged by size: 2626 1697 570 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2574 2820 669 Size: 988 281 636 246 3079 Fragments arranged by size: 3079 988 636 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5230 HpaI GTT'AAC Cuts at: 2820 2820 Size: 5230 KasI G'GCGC_C Cuts at: 2190 2190 Size: 5230 KpnI G_GTAC'C Cuts at: 2555 2555 Size: 5230 MfeI C'AATT_G Cuts at: 2829 2829 Size: 5230 MluI A'CGCG_T Cuts at: 5106 5106 Size: 5230 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4344 4528 1305 Size: 3039 184 2007 Fragments arranged by size: 3039 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3408 Fragments arranged by size: 3408 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3188 3547 3706 518 Size: 648 2022 359 159 2042 Fragments arranged by size: 2042 2022 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2646 3323 4471 4716 1606 Size: 450 590 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 590 450 245 NaeI GCC'GGC Cuts at: 2189 2189 Size: 5230 NarI GG'CG_CC Cuts at: 2191 2191 Size: 5230 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3739 Fragments arranged by size: 3739 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5230 NgoMIV G'CCGG_C Cuts at: 2187 2187 Size: 5230 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5230 NotI GC'GGCC_GC Cuts at: 2677 2677 Size: 5230 NspI r_CATG'y Cuts at: 1132 1229 2588 4133 1132 Size: 97 1359 1545 2229 Fragments arranged by size: 2229 1545 1359 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5230 PciI A'CATG_T Cuts at: 4129 4129 Size: 5230 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5230 PfoI T'CCnGG_A Cuts at: 2160 2289 2160 Size: 129 5101 PmeI GTTT'AAAC Cuts at: 2534 2534 Size: 5230 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3299 3331 4812 4844 3299 Size: 32 1481 32 3685 Fragments arranged by size: 3685 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5230 PsiI TTA'TAA Cuts at: 2800 2800 Size: 5230 PstI C_TGCA'G Cuts at: 830 2493 2582 830 Size: 1663 89 3478 Fragments arranged by size: 3478 1663 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5230 PvuII CAG'CTG Cuts at: 2056 2056 Size: 5230 SacI G_AGCT'C Cuts at: 2273 2273 Size: 5230 SalI G'TCGA_C Cuts at: 1936 2572 1936 Size: 636 4594 SbfI CC_TGCA'GG Cuts at: 2582 2582 Size: 5230 ScaI AGT'ACT Cuts at: 3478 5190 3478 Size: 1712 3518 SfcI C'TryA_G Cuts at: 826 1045 2489 2578 3713 4394 4585 826 Size: 219 1444 89 1135 681 191 1471 Fragments arranged by size: 1471 1444 1135 681 219 191 89 SfoI GGC'GCC Cuts at: 2192 2192 Size: 5230 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5230 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5230 SmaI CCC'GGG Cuts at: 1872 2222 2557 1872 Size: 350 335 4545 Fragments arranged by size: 4545 350 335 SmlI C'TyrA_G Cuts at: 820 1017 1943 2272 2477 3334 4235 4497 820 Size: 197 926 329 205 857 901 262 1553 Fragments arranged by size: 1553 926 901 857 329 262 205 197 SnaBI TAC'GTA Cuts at: 493 3021 493 Size: 2528 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5230 SphI G_CATG'C Cuts at: 1132 2588 1132 Size: 1456 3774 SspI AAT'ATT Cuts at: 5 52 3154 5 Size: 47 3102 2081 Fragments arranged by size: 3102 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5230 StyI C'CwwG_G Cuts at: 513 1065 2004 2669 513 Size: 552 939 665 3074 Fragments arranged by size: 3074 939 665 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2257 3257 3274 3427 3612 4057 325 Size: 1255 677 1000 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1000 677 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2139 3476 5188 Size: 274 80 33 51 1443 162 1337 1712 Cuts at: 5188 96 Size: 138 Fragments arranged by size: 1712 1443 1337 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2064 2096 5190 5222 2064 Size: 32 3094 32 2072 Fragments arranged by size: 3094 2072 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5230 XbaI T'CTAG_A Cuts at: 2566 2566 Size: 5230 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5230 XmaI C'CCGG_G Cuts at: 1870 2220 2555 1870 Size: 350 335 4545 Fragments arranged by size: 4545 350 335 XmnI GAAnn'nnTTC Cuts at: 3359 3359 Size: 5230 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3038 276 Size: 53 83 186 936 1504 2468 Fragments arranged by size: 2468 1504 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI