################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaBI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HindIII HpaI KasI KpnI MamI MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tsp509I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 213 GTATAC GT^[AC][GT]AC AccII 976 CGCG CG^CG AfaI 923 GTAC GT^AC AflIII 962 ACACGT A^C[GA][CT]GT AlwI 268 GATCC GGATC(4/5) AspI 702 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 850 TGCGCA TGC^GCA BalI 665 TGGCCA TGG^CCA BanI 179 GGCACC G^G[CT][GA]CC BfaI 515 CTAG C^TAG BglI 1025 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 636 GCTCAGC GC^T[ACGT]AGC BpmI 286 CTGGAG CTGGAG(16/14) Bpu1102I 636 GCTCAGC GC^T[ACGT]AGC BsaAI 920 CACGTA [CT]AC^GT[GA] BsaWI 753 ACCGGA [AT]^CCGG[AT] BsmBI 246 GAGACG CGTCTC(1/5) Bst1107I 213 GTATAC GTA^TAC BstUI 976 CGCG CG^CG BstYI 267 AGATCC [GA]^GATC[CT] BstZ17I 213 GTATAC GTA^TAC CelII 636 GCTCAGC GC^T[ACGT]AGC DpnII 268 GATC ^GATC DraII 739 AGGCCCC [GA]G^G[ACGT]CC[CT] EagI 911 CGGCCG C^GGCCG EclXI 911 CGGCCG C^GGCCG Eco52I 911 CGGCCG C^GGCCG EcoNI 315 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 739 AGGCCCC [GA]G^G[ACGT]CC[CT] FauI 227 CCCGC CCCGC(4/6) FspI 850 TGCGCA TGC^GCA HincII 966 GTCAAC GT[CT]^[GA]AC HindII 966 GTCAAC GT[CT]^[GA]AC KspI 975 CCGCGG CCGC^GG MaeI 515 CTAG C^TAG MboI 268 GATC ^GATC MflI 267 AGATCC [GA]^GATC[CT] MluNI 665 TGGCCA TGG^CCA MscI 665 TGGCCA TGG^CCA MvnI 976 CGCG CG^CG NdeII 268 GATC ^GATC NlaIII 810 CATG CATG^ NotI 910 GCGGCCGC GC^GGCCGC PflFI 702 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 536 CTGCAG CTGCA^G PvuII 208 CAGCTG CAG^CTG RsaI 923 GTAC GT^AC SacII 975 CCGCGG CCGC^GG Sau3AI 268 GATC ^GATC SfcI 536 CTGCAG C^T[GA][CT]AG SmaI 201 CCCGGG CCC^GGG SmlI 689 CTCAAG C^T[CT][GA]AG StuI 192 AGGCCT AGG^CCT TaqI 1017 TCGA T^CGA Tth111I 702 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1017 TCGA T^CGA XhoII 267 AGATCC [GA]^GATC[CT] XmaCI 201 CCCGGG C^CCGGG XmaI 201 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 559 GACGCC G[GA]^CG[CT]C 1022 GACGCC Aor51HI 30 AGCGCT AGC^GCT 614 AGCGCT AspHI 64 GAGCAC G[AT]GC[AT]^C 342 GAGCAC AvaI 34 CTCGGG C^[CT]CG[GA]G 201 CCCGGG BbsI 574 GAAGAC GAAGAC(2/6) 113 GTCTTC BpuAI 574 GAAGAC GAAGAC(2/6) 113 GTCTTC BsaHI 559 GACGCC G[GA]^CG[CT]C 1022 GACGCC BsgI 253 CTGCAC GTGCAG(16/14) 95 GTGCAG BsiEI 628 CGACCG CG[GA][CT]^CG 911 CGGCCG BsiHKAI 64 GAGCAC G[AT]GC[AT]^C 342 GAGCAC BsmAI 246 GAGAC GTCTC(1/5) 700 GAGAC BsmFI 38 GGGAC GGGAC(10/14) 708 GTCCC BsoBI 34 CTCGGG C^[CT]CG[GA]G 201 CCCGGG BsrI 136 ACTGG ACTGG(1/-1) 42 CCAGT Eco47III 30 AGCGCT AGC^GCT 614 AGCGCT HaeII 30 AGCGCT [GA]GCGC^[CT] 614 AGCGCT Hin1I 559 GACGCC G[GA]^CG[CT]C 1022 GACGCC HinfI 127 GACTC G^A[ACGT]TC 702 GACTC HphI 332 TCACC GGTGA(8/7) 660 TCACC PleI 127 GACTC GAGTC(4/5) 702 GACTC SapI 835 GAAGAGC GCTCTTC(1/4) 1000 GAAGAGC SfaNI 394 GATGC GCATC(5/9) 413 GCATC TspRI 67 CACTG CA[GC]TG[ACGT][ACGT]^ 1029 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AluI AvaII BanII BbvI BcnI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DsaI EaeI EarI EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI Ksp632I MaeII MaeIII MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NlaIV Sau96I ScrFI TseI Tsp45I