(Circular) (Six-base) MAPSORT of: pFN21AE0904.seq Check: 8927 from: 1 to: 5776 pFN21AE0904 5776 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2219 3119 1937 Size: 282 900 4594 Fragments arranged by size: 4594 900 282 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2289 2325 2342 2859 3839 5223 5491 1632 Size: 657 36 17 517 980 1384 268 1917 Fragments arranged by size: 1917 1384 980 657 517 268 36 17 AfeI AGC'GCT Cuts at: 2037 2621 2037 Size: 584 5192 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 2967 4675 5652 1203 Size: 1764 1708 977 1327 Fragments arranged by size: 1764 1708 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 2095 1166 Size: 929 4847 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2758 2790 2758 Size: 32 5744 AlwNI CAG_nnn'CTG Cuts at: 3519 5091 3519 Size: 1572 4204 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 3085 3311 3463 5572 1493 Size: 1592 226 152 2109 1697 Fragments arranged by size: 2109 1697 1592 226 152 AseI AT'TA_AT Cuts at: 160 1032 4330 160 Size: 872 3298 1606 Fragments arranged by size: 3298 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2039 2206 3101 1870 Size: 73 96 167 895 4545 Fragments arranged by size: 4545 895 167 96 73 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2184 3097 4552 618 Size: 325 217 627 397 913 1455 1842 Fragments arranged by size: 1842 1455 913 627 397 325 217 BanII G_rGCy'C Cuts at: 1391 2533 2643 2810 3095 1391 Size: 1142 110 167 285 4072 Fragments arranged by size: 4072 1142 285 167 110 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2111 2586 928 Size: 971 212 475 4118 Fragments arranged by size: 4118 971 475 212 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3036 4387 136 Size: 107 122 71 631 605 1364 1351 1525 Fragments arranged by size: 1525 1364 1351 631 605 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 2642 3193 2642 Size: 551 5225 Bme1580I G_kGCm'C Cuts at: 1790 3837 4993 1790 Size: 2047 1156 2573 Fragments arranged by size: 2573 2047 1156 BmgBI CAC'GTC Cuts at: 1318 2955 2970 1318 Size: 1637 15 4124 Fragments arranged by size: 4124 1637 15 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2151 3507 4465 Size: 416 53 83 399 24 734 1356 958 Cuts at: 4465 442 Size: 1753 Fragments arranged by size: 1753 1356 958 734 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3031 3031 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2312 4436 1239 Size: 1073 2124 2579 Fragments arranged by size: 2579 2124 1073 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2679 3901 4766 5064 2679 Size: 1222 865 298 3391 Fragments arranged by size: 3391 1222 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2927 3567 493 Size: 711 1723 640 2702 Fragments arranged by size: 2702 1723 711 640 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2565 3028 3583 3965 Size: 53 83 186 936 1032 463 555 382 Cuts at: 3965 4600 275 Size: 635 1451 Fragments arranged by size: 1451 1032 936 635 555 463 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2758 4209 4607 4881 5028 5439 2758 Size: 1451 398 274 147 411 3095 Fragments arranged by size: 3095 1451 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2696 2726 1178 Size: 30 165 30 1293 30 4228 Fragments arranged by size: 4228 1293 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2423 2569 2693 2901 1186 Size: 1237 146 124 208 4061 Fragments arranged by size: 4061 1237 208 146 124 BseYI C'CCAG_C Cuts at: 4979 5475 4979 Size: 496 5280 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2121 2243 1163 Size: 573 385 122 4696 Fragments arranged by size: 4696 573 385 122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2636 2919 3226 3987 5015 1540 Size: 275 186 635 283 307 761 1028 2301 Fragments arranged by size: 2301 1028 761 635 307 283 275 186 BsiHKAI G_wGCw'C Cuts at: 2073 2351 3837 3922 4993 2073 Size: 278 1486 85 1071 2856 Fragments arranged by size: 2856 1486 1071 278 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 BsmBI CGTCTCn'nnnn_ Cuts at: 2245 2245 Size: 5776 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2351 2533 2643 2810 3095 3837 Size: 399 283 278 182 110 167 285 742 Cuts at: 3837 3922 4993 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 285 283 278 182 167 110 85 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3131 844 Size: 2287 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2272 3106 3473 3858 3875 5327 5413 5425 Size: 300 834 367 385 17 1452 86 12 Cuts at: 5425 5771 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 834 385 367 346 300 86 17 12 BstZ17I GTA'TAC Cuts at: 2220 2220 Size: 5776 BtgI C'CryG_G Cuts at: 513 1065 2004 2371 2931 2980 513 Size: 552 939 367 560 49 3309 Fragments arranged by size: 3309 939 560 552 367 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2071 3040 3292 4086 4106 2071 Size: 969 252 794 20 3741 Fragments arranged by size: 3741 969 794 252 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2809 2844 529 Size: 35 2245 35 3461 Fragments arranged by size: 3461 2245 35 35 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2670 2916 3037 Size: 54 1425 50 275 18 840 246 121 Cuts at: 3037 3223 4112 5741 8 Size: 186 889 1629 43 Fragments arranged by size: 1629 1425 889 840 275 246 186 121 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2916 3223 1537 Size: 275 1104 307 4090 Fragments arranged by size: 4090 1104 307 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2174 2243 2472 2484 2819 2835 3000 3706 Size: 821 69 229 12 335 16 165 706 Cuts at: 3706 1353 Size: 3423 Fragments arranged by size: 3423 821 706 335 229 165 69 16 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2289 2312 2325 2342 2859 3839 4436 Size: 393 657 23 13 17 517 980 597 Cuts at: 4436 5223 5491 1239 Size: 787 268 1524 Fragments arranged by size: 1524 980 787 657 597 517 393 268 23 17 13 EcoNI CCTnn'n_nnAGG Cuts at: 2324 2324 Size: 5776 EcoO109I rG'GnC_Cy Cuts at: 2745 2745 Size: 5776 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2330 2330 Size: 5776 FspI TGC'GCA Cuts at: 2857 4282 2857 Size: 1425 4351 HaeII r_GCGC'y Cuts at: 1678 2039 2623 4923 1678 Size: 361 584 2300 2531 Fragments arranged by size: 2531 2300 584 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2555 2587 2625 2657 2696 Size: 32 135 32 983 32 38 32 39 Cuts at: 2696 2728 2834 2866 4417 4449 4491 4523 1373 Size: 32 106 32 1551 32 42 32 2626 Fragments arranged by size: 2626 1551 983 135 106 42 39 38 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2973 3120 3366 669 Size: 988 281 1035 147 246 3079 Fragments arranged by size: 3079 1035 988 281 246 147 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2835 4890 5074 1305 Size: 1530 2055 184 2007 Fragments arranged by size: 2055 2007 1530 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2672 10 Size: 54 1425 343 840 3114 Fragments arranged by size: 3114 1425 840 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2095 2171 2730 3734 4093 4252 518 Size: 648 929 76 559 1004 359 159 2042 Fragments arranged by size: 2042 1004 929 648 559 359 159 76 MspA1I CmG'CkG Cuts at: 1606 2215 2235 2915 2982 3192 3869 5017 5262 Size: 609 20 680 67 210 677 1148 245 Cuts at: 5262 1606 Size: 2120 Fragments arranged by size: 2120 1148 680 677 609 245 210 67 20 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4285 Fragments arranged by size: 4285 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 2916 3223 2916 Size: 307 5469 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PfoI T'CCnGG_A Cuts at: 2127 2421 2127 Size: 294 5482 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2147 2179 2147 Size: 32 5744 PstI C_TGCA'G Cuts at: 830 2545 3128 830 Size: 1715 583 3478 Fragments arranged by size: 3478 1715 583 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2215 2215 Size: 5776 SacII CC_GC'GG Cuts at: 2983 2983 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SapI GCTCTTCn'nnn_ Cuts at: 2835 3000 2835 Size: 165 5611 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SfcI C'TryA_G Cuts at: 826 1045 2541 3124 4259 4940 5131 826 Size: 219 1496 583 1135 681 191 1471 Fragments arranged by size: 1496 1471 1135 681 583 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 2208 3103 1872 Size: 336 895 4545 Fragments arranged by size: 4545 895 336 SmlI C'TyrA_G Cuts at: 820 1017 1943 2694 3880 4781 5043 820 Size: 197 926 751 1186 901 262 1553 Fragments arranged by size: 1553 1186 926 901 751 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 2199 1517 Size: 682 5094 StyI C'CwwG_G Cuts at: 513 1065 2004 3215 513 Size: 552 939 1211 3074 Fragments arranged by size: 3074 1211 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2277 2700 2734 3803 3820 3973 4158 Size: 1255 697 423 34 1069 17 153 185 Cuts at: 4158 4603 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1069 697 445 423 185 153 34 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5734 96 Size: 274 80 33 51 1443 2045 1712 138 Fragments arranged by size: 2045 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5736 5768 5736 Size: 32 5744 Tth111I GACn'n_nGTC Cuts at: 1316 2710 1316 Size: 1394 4382 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 2206 3101 1870 Size: 336 895 4545 Fragments arranged by size: 4545 895 336 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI