Restriction map for
pFN21AE6781
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# NO CUT ENZYMES ( 109 enzymes) #
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AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI AspEI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
CelII ClaI CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KspI MfeI
MluI MroI MslI MunI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI TaqI Tsp509I TthHB8I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 24 GACGTC GACGT^C
Acc65I 322 GGTACC G^GTACC
AccII 955 CGCG CG^CG
AlwI 1353 GGATC GGATC(4/5)
AocI 610 CCTGAGG CC^T[ACGT]AGG
Asp700I 648 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 322 GGTACC G^GTACC
AspI 1504 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 377 CCCGAG C^[CT]CG[GA]G
AviII 1274 TGCGCA TGC^GCA
AvrII 705 CCTAGG C^CTAGG
BalI 455 TGGCCA TGG^CCA
BbeI 602 GGCGCC GGCGC^C
BbsI 21 GAAGAC GAAGAC(2/6)
BglII 815 AGATCT A^GATCT
BlnI 705 CCTAGG C^CTAGG
BpuAI 21 GAAGAC GAAGAC(2/6)
BsaBI 810 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1207 GAGACC GGTCTC(1/5)
BseRI 1609 CTCCTC GAGGAG(10/8)
BsoBI 377 CCCGAG C^[CT]CG[GA]G
BsrFI 881 ACCGGC [GA]^CCGG[CT]
BstUI 955 CGCG CG^CG
Bsu36I 610 CCTGAGG CC^T[ACGT]AGG
Cfr10I 881 ACCGGC [GA]^CCGG[CT]
DraI 999 TTTAAA TTT^AAA
DraII 862 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 231 GACTCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1238 CCGTGG C^C[GA][CT]GG
EaeI 455 TGGCCA [CT]^GGCC[GA]
Eco81I 610 CCTGAGG CC^T[ACGT]AGG
EcoO109I 862 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 705 CCTAGG C^C[AT][AT]GG
FspI 1274 TGCGCA TGC^GCA
KasI 602 GGCGCC G^GCGCC
KpnI 322 GGTACC GGTAC^C
MamI 810 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 455 TGGCCA TGG^CCA
MscI 455 TGGCCA TGG^CCA
MseI 1000 TTAA T^TAA
MvnI 955 CGCG CG^CG
NarI 602 GGCGCC GG^CGCC
NheI 489 GCTAGC G^CTAGC
NspI 744 GCATGT [GA]CATG^[CT]
PflFI 1504 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 862 AGGACCC [GA]G^G[AT]CC[CT]
SacI 388 GAGCTC GAGCT^C
SbfI 1617 CCTGCAGG CCTGCA^GG
ScaI 1217 AGTACT AGT^ACT
SfoI 602 GGCGCC GGC^GCC
Sse8387I 1617 CCTGCAGG CCTGCA^GG
StuI 761 AGGCCT AGG^CCT
StyI 705 CCTAGG C^C[AT][AT]GG
Tru9I 1000 TTAA T^TAA
Tth111I 1504 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 648 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 323 GTAC GT^AC
1218 GTAC
BsmI 648 GAATGC GAATGC(1/-1)
988 GAATGC
BsrBI 549 CCGCTC CCGCTC(-3/-3)
418 GAGCGG
BstAPI 1441 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1530 GCAGGAGCTGC
BstEII 1392 GGTCACC G^GT[ACGT]ACC
1624 GGTAACC
BstPI 1392 GGTCACC G^GT[ACGT]ACC
1624 GGTAACC
BstYI 815 AGATCT [GA]^GATC[CT]
1353 GGATCT
DpnII 816 GATC ^GATC
1354 GATC
EcoNI 606 CCTCCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1592 CCTGCCCCAGG
EcoO65I 1392 GGTCACC G^GT[ACGT]ACC
1624 GGTAACC
HaeII 113 AGCGCC [GA]GCGC^[CT]
602 GGCGCC
MaeII 25 ACGT A^CGT
1258 ACGT
MboI 816 GATC ^GATC
1354 GATC
MflI 815 AGATCT [GA]^GATC[CT]
1353 GGATCT
NdeII 816 GATC ^GATC
1354 GATC
RsaI 323 GTAC GT^AC
1218 GTAC
SapI 31 GAAGAGC GCTCTTC(1/4)
484 GAAGAGC
Sau3AI 816 GATC ^GATC
1354 GATC
XcmI 579 CCAGCCGGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1490 CCATAGAGTCCCTGG
XhoII 815 AGATCT [GA]^GATC[CT]
1353 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AcyI AluI AlwNI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV PflMI
PleI PstI PvuII Sau96I ScrFI
SfaNI SfcI SmlI TfiI TseI
Tsp45I TspRI Van91I