(Circular) (Six-base) MAPSORT of: pFN21AE6781.seq Check: 3653 from: 1 to: 6406 pFN21AE6781 6406 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3451 3451 Size: 6406 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2033 4216 278 Size: 53 83 186 936 497 2183 2468 Fragments arranged by size: 2468 2183 936 497 186 83 53 AccI GT'mk_AC Cuts at: 1937 3749 1937 Size: 1812 4594 Acc65I G'GTAC_C Cuts at: 2327 3727 2327 Size: 1400 5006 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2039 2099 2271 3669 4469 5853 6121 1632 Size: 407 60 172 1398 800 1384 268 1917 Fragments arranged by size: 1917 1398 1384 800 407 268 172 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 5305 6282 1203 Size: 4102 977 1327 Fragments arranged by size: 4102 1327 977 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 5135 5135 Size: 6406 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6406 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2758 3308 1184 Size: 1574 550 4282 Fragments arranged by size: 4282 1574 550 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2271 2303 2271 Size: 32 6374 AlwNI CAG_nnn'CTG Cuts at: 2077 2170 2230 3133 3541 4149 5721 2077 Size: 93 60 903 408 608 1572 2762 Fragments arranged by size: 2762 1572 903 608 408 93 60 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 3715 3941 4093 6202 1493 Size: 2222 226 152 2109 1697 Fragments arranged by size: 2222 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 2382 3731 1870 Size: 73 439 1349 4545 Fragments arranged by size: 4545 1349 439 73 AvrII C'CTAG_G Cuts at: 2710 2710 Size: 6406 BamHI G'GATC_C Cuts at: 3736 4103 3736 Size: 367 6039 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2327 2334 2607 3027 3153 Size: 325 217 627 540 7 273 420 126 Cuts at: 3153 3727 5182 618 Size: 574 1455 1842 Fragments arranged by size: 1842 1455 627 574 540 420 325 273 217 126 7 BanII G_rGCy'C Cuts at: 1391 2373 2397 2810 3725 1391 Size: 982 24 413 915 4072 Fragments arranged by size: 4072 982 915 413 24 BbeI G_GCGC'C Cuts at: 2611 2611 Size: 6406 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2033 928 Size: 971 134 5301 Fragments arranged by size: 5301 971 134 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4597 4631 1903 Size: 34 50 34 2576 34 3678 Fragments arranged by size: 3678 2576 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4298 5508 4298 Size: 1210 5196 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 2468 3021 Size: 107 122 71 631 605 430 366 553 Cuts at: 3021 5017 136 Size: 1996 1525 Fragments arranged by size: 1996 1525 631 605 553 430 366 122 107 71 BglII A'GATC_T Cuts at: 2820 6401 2820 Size: 3581 2825 BlpI GC'TnA_GC Cuts at: 3823 3823 Size: 6406 Bme1580I G_kGCm'C Cuts at: 1790 2404 2481 3187 4467 5623 1790 Size: 614 77 706 1280 1156 2573 Fragments arranged by size: 2573 1280 1156 706 614 77 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6406 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2291 4137 5095 Size: 416 53 83 399 24 874 1846 958 Cuts at: 5095 442 Size: 1753 Fragments arranged by size: 1846 1753 958 874 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2498 1056 Size: 1442 4964 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2237 2237 Size: 6406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2252 2312 2411 2432 2660 2794 3155 Size: 968 45 60 99 21 228 134 361 Cuts at: 3155 3317 3548 3566 5066 1239 Size: 162 231 18 1500 2579 Fragments arranged by size: 2579 1500 968 361 231 228 162 134 99 60 45 21 18 Bpu10I CC'TnA_GC Cuts at: 2367 2367 Size: 6406 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3221 3296 3488 4531 5396 5694 3221 Size: 75 192 1043 865 298 3933 Fragments arranged by size: 3933 1043 865 298 192 75 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3206 5069 882 Size: 617 1707 1863 2219 Fragments arranged by size: 2219 1863 1707 617 BsaAI yAC'GTr Cuts at: 493 1204 4197 493 Size: 711 2993 2702 Fragments arranged by size: 2993 2702 711 BsaBI GATnn'nnATC Cuts at: 2819 6400 2819 Size: 3581 2825 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2014 2030 2608 3513 Size: 53 83 186 936 481 16 578 905 Cuts at: 3513 4213 4595 5230 275 Size: 700 382 635 1451 Fragments arranged by size: 1451 936 905 700 635 578 481 382 186 83 53 16 BsaWI w'CCGG_w Cuts at: 4839 5237 5511 5658 6069 4839 Size: 398 274 147 411 5176 Fragments arranged by size: 5176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3011 3041 1178 Size: 30 165 30 1608 30 4543 Fragments arranged by size: 4543 1608 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3605 1186 Size: 2419 3987 BseYI C'CCAG_C Cuts at: 2439 2571 2583 2891 5609 6105 2439 Size: 132 12 308 2718 496 2740 Fragments arranged by size: 2740 2718 496 308 132 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2561 2669 3326 3429 1163 Size: 573 825 108 657 103 4140 Fragments arranged by size: 4140 825 657 573 108 103 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3856 4617 5645 1540 Size: 275 186 1855 761 1028 2301 Fragments arranged by size: 2301 1855 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 2397 3312 3372 3582 4467 4552 5623 2223 Size: 174 915 60 210 885 85 1071 3006 Fragments arranged by size: 3006 1071 915 885 210 174 85 60 BsmI GAATG_Cn' Cuts at: 2659 2999 3917 4010 2659 Size: 340 918 93 5055 Fragments arranged by size: 5055 918 340 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2223 2373 2397 2404 2481 2810 3187 Size: 399 433 150 24 7 77 329 377 Cuts at: 3187 3312 3372 3582 3725 4467 4552 5623 1391 Size: 125 60 210 143 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 433 399 377 329 210 150 143 125 85 77 60 24 7 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 4295 6025 4295 Size: 1730 4676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2922 3285 3451 3630 3761 844 Size: 2078 363 166 179 131 3489 Fragments arranged by size: 3489 2078 363 179 166 131 BsrBI CCG'CTC Cuts at: 1394 1823 2425 2556 4293 1394 Size: 429 602 131 1737 3507 Fragments arranged by size: 3507 1737 602 429 131 BsrDI GCAATG_nn' Cuts at: 65 1248 4901 5075 65 Size: 1183 3653 174 1396 Fragments arranged by size: 3653 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2886 5050 5237 1700 Size: 110 1076 2164 187 2869 Fragments arranged by size: 2869 2164 1076 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6406 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 4466 5478 4466 Size: 1012 5394 BstAPI GCAn_nnn'nTGC Cuts at: 794 3452 3541 794 Size: 2658 89 3659 Fragments arranged by size: 3659 2658 89 BstBI TT'CG_AA Cuts at: 5210 5210 Size: 6406 BstEII G'GTnAC_C Cuts at: 1184 3397 3629 1184 Size: 2213 232 3961 Fragments arranged by size: 3961 2213 232 BstYI r'GATC_y Cuts at: 1972 2820 3358 3736 4103 4488 4505 5957 6043 Size: 848 538 378 367 385 17 1452 86 Cuts at: 6043 6055 6401 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 848 538 385 378 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2616 2616 Size: 6406 BtgI C'CryG_G Cuts at: 513 1065 3243 513 Size: 552 2178 3676 Fragments arranged by size: 3676 2178 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2994 487 Size: 683 56 1768 3899 Fragments arranged by size: 3899 1768 683 56 BtsI GCAGTG_nn' Cuts at: 2434 2735 3582 3922 4716 4736 2434 Size: 301 847 340 794 20 4104 Fragments arranged by size: 4104 847 794 340 301 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6371 DraI TTT'AAA Cuts at: 3006 3710 3006 Size: 704 5702 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2242 5413 809 Size: 507 926 3171 1802 Fragments arranged by size: 3171 1802 926 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2460 3853 4742 Size: 54 1425 50 275 18 630 1393 889 Cuts at: 4742 6371 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1393 889 630 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3853 1537 Size: 275 2041 4090 Fragments arranged by size: 4090 2041 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 2247 2484 2545 4336 1353 Size: 678 216 237 61 1791 3423 Fragments arranged by size: 3423 1791 678 237 216 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2060 4993 5367 5513 387 Size: 1064 138 471 2933 374 146 1280 Fragments arranged by size: 2933 1280 1064 471 374 146 138 EcoICRI GAG'CTC Cuts at: 2395 2395 Size: 6406 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2039 2099 2207 2252 2271 2312 2411 Size: 393 407 60 108 45 19 41 99 Cuts at: 2411 2432 2660 2794 3155 3317 3548 3566 3669 Size: 21 228 134 361 162 231 18 103 Cuts at: 3669 4469 5066 5853 6121 1239 Size: 800 597 787 268 1524 Fragments arranged by size: 1524 800 787 597 407 393 361 268 231 228 162 134 108 103 99 60 45 41 21 19 18 EcoNI CCTnn'n_nnAGG Cuts at: 2615 3601 2615 Size: 986 5420 EcoO109I rG'GnC_Cy Cuts at: 2868 2868 Size: 6406 EcoRI G'AATT_C Cuts at: 3715 4093 3715 Size: 378 6028 FspI TGC'GCA Cuts at: 3281 4912 3281 Size: 1631 4775 HaeII r_GCGC'y Cuts at: 1678 2122 2611 5553 1678 Size: 444 489 2942 2531 Fragments arranged by size: 2942 2531 489 444 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3095 3127 5047 5079 5121 Size: 32 135 32 1523 32 1920 32 42 Cuts at: 5121 5153 1373 Size: 32 2626 Fragments arranged by size: 2626 1920 1523 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3750 3996 669 Size: 988 281 1812 246 3079 Fragments arranged by size: 3079 1812 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6406 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KasI G'GCGC_C Cuts at: 2607 2607 Size: 6406 KpnI G_GTAC'C Cuts at: 2331 3731 2331 Size: 1400 5006 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2556 5520 5704 1305 Size: 1251 2964 184 2007 Fragments arranged by size: 2964 2007 1251 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2462 10 Size: 54 1425 343 630 3954 Fragments arranged by size: 3954 1425 630 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4364 4723 4882 518 Size: 648 3198 359 159 2042 Fragments arranged by size: 3198 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2230 2575 3388 3637 3822 4499 5647 5892 Size: 624 345 813 249 185 677 1148 245 Cuts at: 5892 1606 Size: 2120 Fragments arranged by size: 2120 1148 813 677 624 345 249 245 185 NarI GG'CG_CC Cuts at: 2608 2608 Size: 6406 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5854 NdeI CA'TA_TG Cuts at: 387 387 Size: 6406 NheI G'CTAG_C Cuts at: 1052 2494 1052 Size: 1442 4964 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 2753 3764 5309 1132 Size: 97 1524 1011 1545 2229 Fragments arranged by size: 2229 1545 1524 1011 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6406 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 2170 2199 2230 3649 1108 Size: 1062 29 31 1419 3865 Fragments arranged by size: 3865 1419 1062 31 29 PfoI T'CCnGG_A Cuts at: 2289 2409 2810 2289 Size: 120 401 5885 Fragments arranged by size: 5885 401 120 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3375 3407 4475 4507 5988 6020 3375 Size: 32 1068 32 1481 32 3761 Fragments arranged by size: 3761 1481 1068 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2868 2868 Size: 6406 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 3976 3976 Size: 6406 PstI C_TGCA'G Cuts at: 830 2488 3099 3129 3222 3480 3537 3577 3627 Size: 1658 611 30 93 258 57 40 50 Cuts at: 3627 3758 830 Size: 131 3478 Fragments arranged by size: 3478 1658 611 258 131 93 57 50 40 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 PvuII CAG'CTG Cuts at: 2230 2575 3388 2230 Size: 345 813 5248 Fragments arranged by size: 5248 813 345 SacI G_AGCT'C Cuts at: 2397 2397 Size: 6406 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SapI GCTCTTCn'nnn_ Cuts at: 2031 2484 2031 Size: 453 5953 SbfI CC_TGCA'GG Cuts at: 3627 3758 3627 Size: 131 6275 ScaI AGT'ACT Cuts at: 3224 4654 6366 3224 Size: 1430 1712 3264 Fragments arranged by size: 3264 1712 1430 SfcI C'TryA_G Cuts at: 826 1045 2484 3095 3125 3218 3476 3533 3573 Size: 219 1439 611 30 93 258 57 40 Cuts at: 3573 3623 3754 4889 5570 5761 826 Size: 50 131 1135 681 191 1471 Fragments arranged by size: 1471 1439 1135 681 611 258 219 191 131 93 57 50 40 30 SfoI GGC'GCC Cuts at: 2609 2609 Size: 6406 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 3733 1872 Size: 1861 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3236 3311 3467 4510 5411 5673 Size: 197 926 1293 75 156 1043 901 262 Cuts at: 5673 820 Size: 1553 Fragments arranged by size: 1553 1293 1043 926 901 262 197 156 75 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 2768 1517 Size: 1251 5155 StyI C'CwwG_G Cuts at: 513 1065 2710 3845 513 Size: 552 1645 1135 3074 Fragments arranged by size: 3074 1645 1135 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2114 3416 4433 4450 4603 4788 5233 Size: 1255 534 1302 1017 17 153 185 445 Cuts at: 5233 325 Size: 1498 Fragments arranged by size: 1498 1302 1255 1017 534 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3222 4652 6364 Size: 274 80 33 51 1443 1245 1430 1712 Cuts at: 6364 96 Size: 138 Fragments arranged by size: 1712 1443 1430 1245 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3011 3043 6366 6398 3011 Size: 32 3323 32 3019 Fragments arranged by size: 3323 3019 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3512 1316 Size: 2196 4210 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2591 3502 2591 Size: 911 5495 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 3731 1870 Size: 1861 4545 XmnI GAAnn'nnTTC Cuts at: 2657 4535 2657 Size: 1878 4528 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2031 4214 276 Size: 53 83 186 936 497 2183 2468 Fragments arranged by size: 2468 2183 936 497 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI