################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII AccI AccII AclI AcyI AflII AflIII AgeI AlwNI AocI Aor51HI ApaI AscI AseI AsnI AspI AvaI AviII BamHI BanII BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BglII BlpI Bpu1102I BsaAI BsaHI BsaI BsiEI BsiWI BsmAI BsmBI BsoBI BspDI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoO65I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KspI MflI MluI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 362 GGTACC G^GTACC AccIII 14 TCCGGA T^CCGGA AciI 2186 GCGG CCGC(-3/-1) AhdI 114 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 71 GGATC GGATC(4/5) Asp718I 362 GGTACC G^GTACC AspEI 114 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 357 CCTAGG C^CTAGG BalI 2 TGGCCA TGG^CCA BanI 362 GGTACC G^G[CT][GA]CC BcgI 156 GCAGAAGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 357 CCTAGG C^CTAGG BpmI 1988 CTGGAG CTGGAG(16/14) BsaBI 487 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 14 TCCGGA [AT]^CCGG[AT] BseAI 14 TCCGGA T^CCGGA BseRI 277 GAGGAG GAGGAG(10/8) BspEI 14 TCCGGA T^CCGGA BsrI 1987 ACTGG ACTGG(1/-1) BstXI 1674 CCATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 2 TGGCCA [CT]^GGCC[GA] Eam1105I 114 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 382 GATATC GAT^ATC EcoT22I 2809 ATGCAT ATGCA^T FauI 2186 GCGGG CCCGC(4/6) HapII 15 CCGG C^CGG HincII 2497 GTCAAC GT[CT]^[GA]AC HindII 2497 GTCAAC GT[CT]^[GA]AC HpaII 15 CCGG C^CGG KpnI 362 GGTACC GGTAC^C MaeII 1948 ACGT A^CGT MamI 487 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1593 CAATTG C^AATTG MluNI 2 TGGCCA TGG^CCA MroI 14 TCCGGA T^CCGGA MscI 2 TGGCCA TGG^CCA MspI 15 CCGG C^CGG MunI 1593 CAATTG C^AATTG NsiI 2809 ATGCAT ATGCA^T PflMI 224 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 236 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 409 GAAGAGC GCTCTTC(1/4) SexAI 347 ACCTGGT A^CC[AT]GGT SpeI 646 ACTAGT A^CTAGT Van91I 224 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BbsI 563 GTCTTC GAAGAC(2/6) 1102 GTCTTC BpuAI 563 GTCTTC GAAGAC(2/6) 1102 GTCTTC BstAPI 1001 GCAGTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2177 GCAGGGAATGC Cac8I 426 GCAGGC GC[ACGT]^[ACGT]GC 611 GCACGC DdeI 568 CTTAG C^T[ACGT]AG 2719 CTCAG DpnII 72 GATC ^GATC 478 GATC DraII 32 GGGTCCC [GA]G^G[ACGT]CC[CT] 234 GGGACCT EcoNI 2279 CCTTTAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2615 CCTTCATTAGG EcoO109I 32 GGGTCCC [GA]G^G[ACGT]CC[CT] 234 GGGACCT MboI 72 GATC ^GATC 478 GATC MspA1I 286 CAGCTG C[AC]G^C[GT]G 834 CAGCTG NdeII 72 GATC ^GATC 478 GATC PpuMI 32 GGGTCCC [GA]G^G[AT]CC[CT] 234 GGGACCT PvuII 286 CAGCTG CAG^CTG 834 CAGCTG Sau3AI 72 GATC ^GATC 478 GATC SfcI 339 CTATAG C^T[GA][CT]AG 1917 CTATAG XcmI 108 CCAAGGGACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 454 CCAAACACAGTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AcsI AfaI AluI Alw44I ApaLI ApoI Asp700I AspHI AvaII BbvI BfaI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI BsmI Bsp1286I Bsp1407I BsrDI BsrGI BssKI BstF5I BstNI Cfr13I DraI EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboII MnlI MseI MslI MvaI MwoI NdeI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI