(Circular) (Six-base) MAPSORT of: pFN21AE3331.seq Check: 1515 from: 1 to: 7546 pFN21AE3331 7546 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5356 278 Size: 53 83 186 936 3820 2468 Fragments arranged by size: 3820 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4889 1937 Size: 2952 4594 Acc65I G'GTAC_C Cuts at: 2367 4867 2367 Size: 2500 5046 AclI AA'CG_TT Cuts at: 1563 5673 6046 1563 Size: 4110 373 3063 Fragments arranged by size: 4110 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2842 2926 3094 3514 3598 3682 4102 4522 Size: 1210 84 168 420 84 84 420 420 Cuts at: 4522 5609 6993 7261 1632 Size: 1087 1384 268 1917 Fragments arranged by size: 1917 1384 1210 1087 420 420 420 268 168 84 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7349 AflIII A'CryG_T Cuts at: 1203 4843 6445 7422 1203 Size: 3640 1602 977 1327 Fragments arranged by size: 3640 1602 1327 977 AgeI A'CCGG_T Cuts at: 6377 6377 Size: 7546 AhdI GACnn_n'nnGTC Cuts at: 2124 6275 2124 Size: 4151 3395 AleI CACnn'nnGTG Cuts at: 1166 2311 3069 4177 1166 Size: 1145 758 1108 4535 Fragments arranged by size: 4535 1145 1108 758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2329 2913 1184 Size: 1145 584 5817 Fragments arranged by size: 5817 1145 584 AlwNI CAG_nnn'CTG Cuts at: 5289 6861 5289 Size: 1572 5974 ApaLI G'TGCA_C Cuts at: 3986 4728 4768 5603 6759 3986 Size: 742 40 835 1156 4773 Fragments arranged by size: 4773 1156 835 742 40 ApoI r'AATT_y Cuts at: 1493 2976 3060 3228 4236 4630 4855 5081 5233 Size: 1483 84 168 1008 394 225 226 152 Cuts at: 5233 7342 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1483 1008 394 226 225 168 152 84 AseI AT'TA_AT Cuts at: 160 1032 6100 160 Size: 872 5068 1606 Fragments arranged by size: 5068 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 AvaI C'yCGr_G Cuts at: 1870 1943 4871 1870 Size: 73 2928 4545 Fragments arranged by size: 4545 2928 73 AvrII C'CTAG_G Cuts at: 2362 2362 Size: 7546 BamHI G'GATC_C Cuts at: 4876 5243 4876 Size: 367 7179 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2367 4867 6322 618 Size: 325 217 627 580 2500 1455 1842 Fragments arranged by size: 2500 1842 1455 627 580 325 217 BanII G_rGCy'C Cuts at: 1391 4865 1391 Size: 3474 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2561 3100 928 Size: 971 662 539 5374 Fragments arranged by size: 5374 971 662 539 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2150 2184 5737 5771 1903 Size: 34 213 34 3553 34 3678 Fragments arranged by size: 3678 3553 213 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5438 6648 5438 Size: 1210 6336 BclI T'GATC_A Cuts at: 1548 1548 Size: 7546 BfrBI ATG'CAT Cuts at: 4816 4816 Size: 7546 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6157 136 Size: 107 122 71 631 605 4485 1525 Fragments arranged by size: 4485 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7541 7541 Size: 7546 BlpI GC'TnA_GC Cuts at: 4963 4963 Size: 7546 Bme1580I G_kGCm'C Cuts at: 1790 3990 4732 4772 5607 6763 1790 Size: 2200 742 40 835 1156 2573 Fragments arranged by size: 2573 2200 1156 835 742 40 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7546 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5277 6235 442 Size: 416 53 83 399 24 3860 958 1753 Fragments arranged by size: 3860 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7546 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3599 3599 Size: 7546 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4014 6206 1239 Size: 2775 2192 2579 Fragments arranged by size: 2775 2579 2192 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5671 6536 6834 5671 Size: 865 298 6383 Fragments arranged by size: 6383 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6209 882 Size: 617 4710 2219 Fragments arranged by size: 4710 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5337 493 Size: 711 4133 2702 Fragments arranged by size: 4133 2702 711 BsaBI GATnn'nnATC Cuts at: 2496 7540 2496 Size: 5044 2502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5353 5735 6370 275 Size: 53 83 186 936 3820 382 635 1451 Fragments arranged by size: 3820 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2019 5979 6377 6651 6798 7209 2019 Size: 3960 398 274 147 411 2356 Fragments arranged by size: 3960 2356 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7321 Fragments arranged by size: 7321 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 1186 Size: 1111 6435 BseYI C'CCAG_C Cuts at: 2302 2518 4047 6749 7245 2302 Size: 216 1529 2702 496 2603 Fragments arranged by size: 2702 2603 1529 496 216 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2450 4201 4369 1163 Size: 573 714 1751 168 4340 Fragments arranged by size: 4340 1751 714 573 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4996 5757 6785 1540 Size: 275 186 2995 761 1028 2301 Fragments arranged by size: 2995 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3990 4732 4772 5607 5692 6763 3990 Size: 742 40 835 85 1071 4773 Fragments arranged by size: 4773 1071 835 742 85 40 BsmI GAATG_Cn' Cuts at: 2549 3008 3017 3260 3596 3680 3764 4193 4268 Size: 459 9 243 336 84 84 429 75 Cuts at: 4268 4578 4604 5057 5150 2549 Size: 310 26 453 93 4945 Fragments arranged by size: 4945 459 453 429 336 310 243 93 84 84 75 26 9 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3990 4732 4772 4865 5607 5692 6763 Size: 399 2200 742 40 93 742 85 1071 Cuts at: 6763 1391 Size: 2174 Fragments arranged by size: 2200 2174 1071 742 742 399 93 85 40 BspEI T'CCGG_A Cuts at: 2019 7209 2019 Size: 5190 2356 BspHI T'CATG_A Cuts at: 5435 7165 5435 Size: 1730 5816 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4901 844 Size: 4057 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5433 1394 Size: 429 3610 3507 Fragments arranged by size: 3610 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3265 3517 4693 6041 6215 65 Size: 1183 2017 252 1176 1348 174 1396 Fragments arranged by size: 2017 1396 1348 1183 1176 252 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6190 6377 1700 Size: 110 4380 187 2869 Fragments arranged by size: 4380 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2319 2965 3385 3469 3889 3973 4057 4477 Size: 2223 646 420 84 420 84 84 420 Cuts at: 4477 4561 4645 96 Size: 84 84 2997 Fragments arranged by size: 2997 2223 646 420 420 420 84 84 84 84 84 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7546 BssSI C'ACGA_G Cuts at: 5606 6618 5606 Size: 1012 6534 BstAPI GCAn_nnn'nTGC Cuts at: 794 3012 4188 794 Size: 2218 1176 4152 Fragments arranged by size: 4152 2218 1176 BstBI TT'CG_AA Cuts at: 6350 6350 Size: 7546 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7546 BstXI CCAn_nnnn'nTGG Cuts at: 3686 3686 Size: 7546 BstYI r'GATC_y Cuts at: 1972 4876 5243 5628 5645 7097 7183 7195 7541 Size: 2904 367 385 17 1452 86 12 346 Cuts at: 7541 1972 Size: 1977 Fragments arranged by size: 2904 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 6055 Fragments arranged by size: 6055 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6807 Fragments arranged by size: 6807 683 56 BtsI GCAGTG_nn' Cuts at: 3013 4105 4273 4357 4609 5062 5856 5876 3013 Size: 1092 168 84 252 453 794 20 4683 Fragments arranged by size: 4683 1092 794 453 252 168 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7546 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3499 3534 3583 3618 4675 4710 529 Size: 35 2935 35 49 35 1057 35 3365 Fragments arranged by size: 3365 2935 1057 49 35 35 35 35 DraI TTT'AAA Cuts at: 3201 3622 3789 4850 3201 Size: 421 167 1061 5897 Fragments arranged by size: 5897 1061 421 167 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6553 809 Size: 507 5237 1802 Fragments arranged by size: 5237 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 4993 5882 Size: 54 1425 50 275 18 177 2986 889 Cuts at: 5882 7511 8 Size: 1629 43 Fragments arranged by size: 2986 1629 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4993 1537 Size: 275 3181 4090 Fragments arranged by size: 4090 3181 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2159 2409 2898 5476 1353 Size: 806 250 489 2578 3423 Fragments arranged by size: 3423 2578 806 489 250 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6133 6507 6653 387 Size: 1064 138 4544 374 146 1280 Fragments arranged by size: 4544 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2842 2926 3094 3514 3598 3682 4014 Size: 393 1210 84 168 420 84 84 332 Cuts at: 4014 4102 4522 5609 6206 6993 7261 1239 Size: 88 420 1087 597 787 268 1524 Fragments arranged by size: 1524 1210 1087 787 597 420 420 393 332 268 168 88 84 84 84 EcoNI CCTnn'n_nnAGG Cuts at: 4288 4624 4288 Size: 336 7210 EcoO109I rG'GnC_Cy Cuts at: 2038 2240 2038 Size: 202 7344 EcoRI G'AATT_C Cuts at: 2976 3060 3228 4236 4630 4855 5233 2976 Size: 84 168 1008 394 225 378 5289 Fragments arranged by size: 5289 1008 394 378 225 168 84 EcoRV GAT'ATC Cuts at: 2389 2389 Size: 7546 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3143 3143 Size: 7546 FspI TGC'GCA Cuts at: 6052 6052 Size: 7546 HaeII r_GCGC'y Cuts at: 1678 6693 1678 Size: 5015 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2110 2142 3877 3909 6187 Size: 32 135 32 538 32 1735 32 2278 Cuts at: 6187 6219 6261 6293 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2278 1735 538 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4504 4890 5136 669 Size: 988 281 2566 386 246 3079 Fragments arranged by size: 3079 2566 988 386 281 246 HindIII A'AGCT_T Cuts at: 748 2536 3189 4365 748 Size: 1788 653 1176 3929 Fragments arranged by size: 3929 1788 1176 653 HpaI GTT'AAC Cuts at: 5136 5136 Size: 7546 KpnI G_GTAC'C Cuts at: 2371 4871 2371 Size: 2500 5046 MfeI C'AATT_G Cuts at: 3598 5145 3598 Size: 1547 5999 MluI A'CGCG_T Cuts at: 7422 7422 Size: 7546 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2316 2382 3683 4775 6660 6844 1305 Size: 1011 66 1301 1092 1885 184 2007 Fragments arranged by size: 2007 1885 1301 1092 1011 184 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 5547 Fragments arranged by size: 5547 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2258 2311 2610 3069 3169 3253 3491 Size: 648 1092 53 299 459 100 84 238 Cuts at: 3491 3684 3841 4093 4177 4413 4429 4597 4833 Size: 193 157 252 84 236 16 168 236 Cuts at: 4833 5504 5863 6022 518 Size: 671 359 159 2042 Fragments arranged by size: 2042 1092 671 648 459 359 299 252 238 236 236 193 168 159 157 100 84 84 53 16 MspA1I CmG'CkG Cuts at: 1606 2293 2841 4962 5639 6787 7032 1606 Size: 687 548 2121 677 1148 245 2120 Fragments arranged by size: 2121 2120 1148 687 677 548 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6055 Fragments arranged by size: 6055 939 552 NdeI CA'TA_TG Cuts at: 387 3166 3250 4090 4146 4426 4594 387 Size: 2779 84 840 56 280 168 3339 Fragments arranged by size: 3339 2779 840 280 168 84 56 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7546 NotI GC'GGCC_GC Cuts at: 4993 4993 Size: 7546 NsiI A_TGCA'T Cuts at: 4818 4818 Size: 7546 NspI r_CATG'y Cuts at: 1132 1229 4847 4904 6449 1132 Size: 97 3618 57 1545 2229 Fragments arranged by size: 3618 2229 1545 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7546 PciI A'CATG_T Cuts at: 4843 6445 4843 Size: 1602 5944 PflMI CCAn_nnn'nTGG Cuts at: 1108 2235 1108 Size: 1127 6419 PmeI GTTT'AAAC Cuts at: 4850 4850 Size: 7546 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5615 5647 7128 7160 5615 Size: 32 1481 32 6001 Fragments arranged by size: 6001 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2038 2240 2038 Size: 202 7344 PshAI GACnn'nnGTC Cuts at: 1613 2245 1613 Size: 632 6914 PsiI TTA'TAA Cuts at: 4512 5116 4512 Size: 604 6942 PstI C_TGCA'G Cuts at: 830 4898 830 Size: 4068 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7546 PvuII CAG'CTG Cuts at: 2293 2841 2293 Size: 548 6998 SalI G'TCGA_C Cuts at: 1936 4888 1936 Size: 2952 4594 SanDI GG'GwC_CC Cuts at: 2038 2038 Size: 7546 SapI GCTCTTCn'nnn_ Cuts at: 2409 2409 Size: 7546 SbfI CC_TGCA'GG Cuts at: 4898 4898 Size: 7546 ScaI AGT'ACT Cuts at: 5794 7506 5794 Size: 1712 5834 SexAI A'CCwGG_T Cuts at: 2352 2352 Size: 7546 SfcI C'TryA_G Cuts at: 826 1045 2344 3922 4894 6029 6710 6901 826 Size: 219 1299 1578 972 1135 681 191 1471 Fragments arranged by size: 1578 1471 1299 1135 972 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7546 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7546 SmaI CCC'GGG Cuts at: 1872 4873 1872 Size: 3001 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5650 6551 6813 820 Size: 197 926 3707 901 262 1553 Fragments arranged by size: 3707 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5337 493 Size: 4844 2702 SpeI A'CTAG_T Cuts at: 152 2651 152 Size: 2499 5047 SphI G_CATG'C Cuts at: 1132 4904 1132 Size: 3772 3774 SspI AAT'ATT Cuts at: 5 52 5470 5 Size: 47 5418 2081 Fragments arranged by size: 5418 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7546 StyI C'CwwG_G Cuts at: 513 1065 2004 2089 2113 2362 4985 513 Size: 552 939 85 24 249 2623 3074 Fragments arranged by size: 3074 2623 939 552 249 85 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2164 3411 4335 4671 5573 5590 5743 Size: 1255 584 1247 924 336 902 17 153 Cuts at: 5743 5928 6373 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1247 924 902 584 445 336 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2319 2576 2965 Size: 274 80 33 51 1443 342 257 389 Cuts at: 2965 3385 3469 3889 3973 4057 4477 4561 4645 Size: 420 84 420 84 84 420 84 84 Cuts at: 4645 5792 7504 96 Size: 1147 1712 138 Fragments arranged by size: 1712 1443 1147 420 420 420 389 342 274 257 138 84 84 84 84 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7506 7538 7506 Size: 32 7514 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7546 XbaI T'CTAG_A Cuts at: 4882 4882 Size: 7546 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2120 2466 2120 Size: 346 7200 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7546 XmaI C'CCGG_G Cuts at: 1870 4871 1870 Size: 3001 4545 XmnI GAAnn'nnTTC Cuts at: 2062 2855 2939 3023 3107 3228 3611 3695 4115 Size: 793 84 84 84 121 383 84 420 Cuts at: 4115 4408 4451 4535 4660 4703 5675 2062 Size: 293 43 84 125 43 972 3933 Fragments arranged by size: 3933 972 793 420 383 293 125 121 84 84 84 84 84 43 43 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5354 276 Size: 53 83 186 936 3820 2468 Fragments arranged by size: 3820 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI