(Linear) (Six-base) MAPSORT of: KIEE3331.seq Check: 10 from: 1 to: 2841 KIEE3331.orf With 182 enzymes: SgfI * December 27, 2011 16:35 .. Acc65I G'GTAC_C Cuts at: 0 362 2841 Size: 362 2479 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 837 921 1089 1509 1593 1677 2097 2517 Size: 837 84 168 420 84 84 420 420 Cuts at: 2517 2841 Size: 324 Fragments arranged by size: 837 420 420 420 324 168 84 84 84 AhdI GACnn_n'nnGTC Cuts at: 0 119 2841 Size: 119 2722 AleI CACnn'nnGTG Cuts at: 0 306 1064 2172 2841 Size: 306 758 1108 669 Fragments arranged by size: 1108 758 669 306 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 324 908 2841 Size: 324 584 1933 Fragments arranged by size: 1933 584 324 ApaLI G'TGCA_C Cuts at: 0 1981 2723 2763 2841 Size: 1981 742 40 78 Fragments arranged by size: 1981 742 78 40 ApoI r'AATT_y Cuts at: 0 971 1055 1223 2231 2625 2841 Size: 971 84 168 1008 394 216 Fragments arranged by size: 1008 971 394 216 168 84 AvrII C'CTAG_G Cuts at: 0 357 2841 Size: 357 2484 BanI G'GyrC_C Cuts at: 0 362 2841 Size: 362 2479 BbsI GAAGACnn'nnnn_ Cuts at: 0 556 1095 2841 Size: 556 539 1746 Fragments arranged by size: 1746 556 539 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 145 179 2841 Size: 145 34 2662 Fragments arranged by size: 2662 145 34 BfrBI ATG'CAT Cuts at: 0 2811 2841 Size: 2811 30 Bme1580I G_kGCm'C Cuts at: 0 1985 2727 2767 2841 Size: 1985 742 40 74 Fragments arranged by size: 1985 742 74 40 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1594 2841 Size: 1594 1247 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2009 2841 Size: 2009 832 BsaBI GATnn'nnATC Cuts at: 0 491 2841 Size: 491 2350 BsaWI w'CCGG_w Cuts at: 0 14 2841 Size: 14 2827 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 292 2841 Size: 292 2549 BseYI C'CCAG_C Cuts at: 0 297 513 2042 2841 Size: 297 216 1529 799 Fragments arranged by size: 1529 799 297 216 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 445 2196 2364 2841 Size: 445 1751 168 477 Fragments arranged by size: 1751 477 445 168 BsiHKAI G_wGCw'C Cuts at: 0 1985 2727 2767 2841 Size: 1985 742 40 74 Fragments arranged by size: 1985 742 74 40 BsmI GAATG_Cn' Cuts at: 0 544 1003 1012 1255 1591 1675 1759 2188 Size: 544 459 9 243 336 84 84 429 Cuts at: 2188 2263 2573 2599 2841 Size: 75 310 26 242 Fragments arranged by size: 544 459 429 336 310 243 242 84 84 75 26 9 Bsp1286I G_dGCh'C Cuts at: 0 1985 2727 2767 2841 Size: 1985 742 40 74 Fragments arranged by size: 1985 742 74 40 BspEI T'CCGG_A Cuts at: 0 14 2841 Size: 14 2827 BsrDI GCAATG_nn' Cuts at: 0 1260 1512 2688 2841 Size: 1260 252 1176 153 Fragments arranged by size: 1260 1176 252 153 BsrGI T'GTAC_A Cuts at: 0 314 960 1380 1464 1884 1968 2052 2472 Size: 314 646 420 84 420 84 84 420 Cuts at: 2472 2556 2640 2841 Size: 84 84 201 Fragments arranged by size: 646 420 420 420 314 201 84 84 84 84 84 BstAPI GCAn_nnn'nTGC Cuts at: 0 1007 2183 2841 Size: 1007 1176 658 Fragments arranged by size: 1176 1007 658 BstXI CCAn_nnnn'nTGG Cuts at: 0 1681 2841 Size: 1681 1160 BtsI GCAGTG_nn' Cuts at: 0 1008 2100 2268 2352 2604 2841 Size: 1008 1092 168 84 252 237 Fragments arranged by size: 1092 1008 252 237 168 84 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1494 1529 1578 1613 2670 2705 2841 Size: 1494 35 49 35 1057 35 136 Fragments arranged by size: 1494 1057 136 49 35 35 35 DraI TTT'AAA Cuts at: 0 1196 1617 1784 2841 Size: 1196 421 167 1057 Fragments arranged by size: 1196 1057 421 167 EaeI y'GGCC_r Cuts at: 0 2 2841 Size: 2 2839 EarI CTCTTCn'nnn_ Cuts at: 0 154 404 893 2841 Size: 154 250 489 1948 Fragments arranged by size: 1948 489 250 154 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 837 921 1089 1509 1593 1677 2009 2097 Size: 837 84 168 420 84 84 332 88 Cuts at: 2097 2517 2841 Size: 420 324 Fragments arranged by size: 837 420 420 332 324 168 88 84 84 84 EcoNI CCTnn'n_nnAGG Cuts at: 0 2283 2619 2841 Size: 2283 336 222 Fragments arranged by size: 2283 336 222 EcoO109I rG'GnC_Cy Cuts at: 0 33 235 2841 Size: 33 202 2606 Fragments arranged by size: 2606 202 33 EcoRI G'AATT_C Cuts at: 0 971 1055 1223 2231 2625 2841 Size: 971 84 168 1008 394 216 Fragments arranged by size: 1008 971 394 216 168 84 EcoRV GAT'ATC Cuts at: 0 384 2841 Size: 384 2457 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1138 2841 Size: 1138 1703 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 105 137 1872 1904 2841 Size: 105 32 1735 32 937 Fragments arranged by size: 1735 937 105 32 32 HincII GTy'rAC Cuts at: 0 2499 2841 Size: 2499 342 HindIII A'AGCT_T Cuts at: 0 531 1184 2360 2841 Size: 531 653 1176 481 Fragments arranged by size: 1176 653 531 481 KpnI G_GTAC'C Cuts at: 0 366 2841 Size: 366 2475 MfeI C'AATT_G Cuts at: 0 1593 2841 Size: 1593 1248 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 311 377 1678 2770 2841 Size: 311 66 1301 1092 71 Fragments arranged by size: 1301 1092 311 71 66 MscI TGG'CCA Cuts at: 0 4 2841 Size: 4 2837 MslI CAynn'nnrTG Cuts at: 0 253 306 605 1064 1164 1248 1486 1679 Size: 253 53 299 459 100 84 238 193 Cuts at: 1679 1836 2088 2172 2408 2424 2592 2828 2841 Size: 157 252 84 236 16 168 236 13 Fragments arranged by size: 459 299 253 252 238 236 236 193 168 157 100 84 84 53 16 13 MspA1I CmG'CkG Cuts at: 0 288 836 2841 Size: 288 548 2005 Fragments arranged by size: 2005 548 288 NdeI CA'TA_TG Cuts at: 0 1161 1245 2085 2141 2421 2589 2841 Size: 1161 84 840 56 280 168 252 Fragments arranged by size: 1161 840 280 252 168 84 56 NsiI A_TGCA'T Cuts at: 0 2813 2841 Size: 2813 28 PflMI CCAn_nnn'nTGG Cuts at: 0 230 2841 Size: 230 2611 PpuMI rG'GwC_Cy Cuts at: 0 33 235 2841 Size: 33 202 2606 Fragments arranged by size: 2606 202 33 PshAI GACnn'nnGTC Cuts at: 0 240 2841 Size: 240 2601 PsiI TTA'TAA Cuts at: 0 2507 2841 Size: 2507 334 PvuII CAG'CTG Cuts at: 0 288 836 2841 Size: 288 548 2005 Fragments arranged by size: 2005 548 288 SanDI GG'GwC_CC Cuts at: 0 33 2841 Size: 33 2808 SapI GCTCTTCn'nnn_ Cuts at: 0 404 2841 Size: 404 2437 SexAI A'CCwGG_T Cuts at: 0 347 2841 Size: 347 2494 SfcI C'TryA_G Cuts at: 0 339 1917 2841 Size: 339 1578 924 Fragments arranged by size: 1578 924 339 SpeI A'CTAG_T Cuts at: 0 646 2841 Size: 646 2195 StyI C'CwwG_G Cuts at: 0 84 108 357 2841 Size: 84 24 249 2484 Fragments arranged by size: 2484 249 84 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 159 1406 2330 2666 2841 Size: 159 1247 924 336 175 Fragments arranged by size: 1247 924 336 175 159 TatI w'GTAC_w Cuts at: 0 314 571 960 1380 1464 1884 1968 2052 Size: 314 257 389 420 84 420 84 84 Cuts at: 2052 2472 2556 2640 2841 Size: 420 84 84 201 Fragments arranged by size: 420 420 420 389 314 257 201 84 84 84 84 84 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 115 461 2841 Size: 115 346 2380 Fragments arranged by size: 2380 346 115 XmnI GAAnn'nnTTC Cuts at: 0 57 850 934 1018 1102 1223 1606 1690 Size: 57 793 84 84 84 121 383 84 Cuts at: 1690 2110 2403 2446 2530 2655 2698 2841 Size: 420 293 43 84 125 43 143 Fragments arranged by size: 793 420 383 293 143 125 121 84 84 84 84 84 57 43 43 Enzymes that do cut: Acc65I AcuI AhdI AleI AlfI ApaLI ApoI AvrII BanI BbsI BcgI BfrBI Bme1580I BplI BpmI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrDI BsrGI BstAPI BstXI BtsI CspCI DraI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I NdeI NsiI PflMI PpuMI PshAI PsiI PvuII SanDI SapI SexAI SfcI SpeI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AloI AlwNI ApaI AscI AseI AsiSI AvaI BaeI BamHI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI ClaI DraIII DrdI EagI EciI EcoICRI FseI FspI FspAI HaeII HpaI KasI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI ZraI