################################## # NO CUT ENZYMES ( 200 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MslI MspI MunI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SbfI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 158 GTAGAC GT^[AC][GT]AC AciI 26 GCGG CCGC(-3/-1) AluI 231 AGCT AG^CT BalI 544 TGGCCA TGG^CCA BanI 463 GGTGCC G^G[CT][GA]CC BbsI 324 GAAGAC GAAGAC(2/6) BbvI 4 GCTGC GCAGC(8/12) BglII 573 AGATCT A^GATCT BpuAI 324 GAAGAC GAAGAC(2/6) BsaI 44 GAGACC GGTCTC(1/5) BsaJI 49 CCAAGG C^C[ACGT][ACGT]GG BseRI 271 GAGGAG GAGGAG(10/8) BsmAI 44 GAGAC GTCTC(1/5) BstF5I 483 GGATG GGATG(2/0) BstYI 573 AGATCT [GA]^GATC[CT] DdeI 468 CTCAG C^T[ACGT]AG EaeI 544 TGGCCA [CT]^GGCC[GA] EcoT14I 49 CCAAGG C^C[AT][AT]GG FauI 26 GCGGG CCCGC(4/6) Fnu4HI 4 GCTGC GC^[ACGT]GC FokI 483 GGATG GGATG(9/13) HphI 217 TCACC GGTGA(8/7) ItaI 4 GCTGC GC^[ACGT]GC MflI 573 AGATCT [GA]^GATC[CT] MluNI 544 TGGCCA TGG^CCA MscI 544 TGGCCA TGG^CCA MseI 422 TTAA T^TAA MspA1I 24 CAGCGG C[AC]G^C[GT]G NlaIV 463 GGTGCC GG[ACGT]^[ACGT]CC ScaI 125 AGTACT AGT^ACT StyI 49 CCAAGG C^C[AT][AT]GG TfiI 19 GAATC G^A[AT]TC Tru9I 422 TTAA T^TAA TseI 4 GCTGC G^C[AT]GC Tsp45I 382 GTGAC ^GT[GC]AC TspRI 57 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoII 573 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 126 GTAC GT^AC 331 GTAC DpnII 418 GATC ^GATC 574 GATC EarI 268 GAAGAG CTCTTC(1/4) 288 GAAGAG HaeIII 397 GGCC GG^CC 545 GGCC Ksp632I 268 GAAGAG CTCTTC(1/4) 288 GAAGAG MboI 418 GATC ^GATC 574 GATC NdeII 418 GATC ^GATC 574 GATC PleI 89 GAGTC GAGTC(4/5) 104 GAGTC RsaI 126 GTAC GT^AC 331 GTAC Sau3AI 418 GATC ^GATC 574 GATC SfaNI 70 GATGC GCATC(5/9) 566 GCATC SfcI 206 CTGTAG C^T[GA][CT]AG 347 CTATAG TaqI 87 TCGA T^CGA 569 TCGA TthHB8I 87 TCGA T^CGA 569 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### AcsI ApoI HinfI MaeIII MboII MnlI Tsp509I