################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 336 CCGC CCGC(-3/-1) AfaI 18 GTAC GT^AC AflIII 416 ACGTGT A^C[GA][CT]GT AhdI 181 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 535 GTGCAC G^TGCAC AlwNI 550 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 535 GTGCAC G^TGCAC AspEI 181 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 535 GTGCAC G[AT]GC[AT]^C AvaII 363 GGTCC G^G[AT]CC BfaI 715 CTAG C^TAG BglII 657 AGATCT A^GATCT BsaI 814 GAGACC GGTCTC(1/5) BseRI 174 CTCCTC GAGGAG(10/8) BsiHKAI 535 GTGCAC G[AT]GC[AT]^C BsmAI 814 GAGAC GTCTC(1/5) BspHI 53 TCATGA T^CATGA BspMI 738 ACCTGC ACCTGC(4/8) BstAPI 528 GCAAGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cac8I 528 GCAAGC GC[ACGT]^[ACGT]GC CfoI 489 GCGC GCG^C DraIII 94 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 683 CCATGG C^C[GA][CT]GG Eam1105I 181 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 618 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 488 GGCGCT [GA]GCGC^[CT] HapII 811 CCGG C^CGG HgaI 793 GACGC GACGC(5/10) HhaI 489 GCGC GCG^C HinP1I 489 GCGC G^CGC HpaII 811 CCGG C^CGG MaeI 715 CTAG C^TAG MaeII 416 ACGT A^CGT MspI 811 CCGG C^CGG NcoI 683 CCATGG C^CATGG NdeI 113 CATATG CA^TATG NspI 32 ACATGC [GA]CATG^[CT] PpuMI 362 AGGTCCT [GA]G^G[AT]CC[CT] PshAI 126 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 445 CTGCAG CTGCA^G RcaI 53 TCATGA T^CATGA RsaI 18 GTAC GT^AC SmlI 970 CTTGAG C^T[CT][GA]AG TaqI 381 TCGA T^CGA TfiI 160 GAATC G^A[AT]TC Tsp509I 197 AATT ^AATT TthHB8I 381 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 768 GATCC GGATC(4/5) 952 GATCC BanII 314 GGGCTC G[GA]GC[CT]^C 385 GGGCTC BsmI 276 GAATGC GAATGC(1/-1) 351 GAATGC BstXI 248 CCAAGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 653 CCATAGATCTGG BstYI 657 AGATCT [GA]^GATC[CT] 951 AGATCC DraII 362 AGGTCCT [GA]G^G[ACGT]CC[CT] 702 GGGGCCT EcoO109I 362 AGGTCCT [GA]G^G[ACGT]CC[CT] 702 GGGGCCT MaeIII 213 GTCAC ^GT[ACGT]AC 854 GTGAC MboII 862 GAAGA GAAGA(8/7) 378 TCTTC MflI 657 AGATCT [GA]^GATC[CT] 951 AGATCC MseI 734 TTAA T^TAA 745 TTAA MspA1I 448 CAGCTG C[AC]G^C[GT]G 550 CAGCTG MwoI 528 GCAAGCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 718 GCGATCAGTGC PleI 47 GACTC GAGTC(4/5) 966 GAGTC PvuII 448 CAGCTG CAG^CTG 550 CAGCTG Tru9I 734 TTAA T^TAA 745 TTAA Tsp45I 213 GTCAC ^GT[GC]AC 854 GTGAC XhoII 657 AGATCT [GA]^GATC[CT] 951 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AluI BbvI BmyI BpmI BsaJI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MboI MnlI MvaI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI TspRI