Restriction map for
pFN21AE4015
##################################
# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AlwI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspI AvaII AviII AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BcgI BciVI BclI BfrI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsiYI
BslI BsmBI Bsp1407I BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstYI BstZ17I Bsu36I CfoI
Cfr10I Cfr13I CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NlaIII NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
Van91I XbaI XcmI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcsI 297 AAATTT [GA]^AATT[CT]
AfaI 423 GTAC GT^AC
AhdI 205 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 208 AGCT AG^CT
Alw44I 448 GTGCAC G^TGCAC
ApaLI 448 GTGCAC G^TGCAC
ApoI 297 AAATTT [GA]^AATT[CT]
AspEI 205 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 150 CTCGAG C^[CT]CG[GA]G
BanI 542 GGTGCC G^G[CT][GA]CC
BbvCI 141 GCTGAGG CCTCAGC(-5/-2)
BbvI 61 GCAGC GCAGC(8/12)
BcnI 185 CCCGG CC^[GC]GG
BfaI 34 CTAG C^TAG
BlpI 81 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 81 GCTGAGC GC^T[ACGT]AGC
BsaJI 537 CCAAGG C^C[ACGT][ACGT]GG
BsmAI 14 GTCTC GTCTC(1/5)
BsmFI 183 GTCCC GGGAC(10/14)
BsmI 388 GAATGC GAATGC(1/-1)
BsoBI 150 CTCGAG C^[CT]CG[GA]G
BspDI 509 ATCGAT AT^CGAT
BsrBI 118 GAGCGG CCGCTC(-3/-3)
BstEII 160 GGTCACC G^GT[ACGT]ACC
BstPI 160 GGTCACC G^GT[ACGT]ACC
CelII 81 GCTGAGC GC^T[ACGT]AGC
ClaI 509 ATCGAT AT^CGAT
DpnII 508 GATC ^GATC
Eam1105I 205 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 160 GGTCACC G^GT[ACGT]ACC
EcoT14I 537 CCAAGG C^C[AT][AT]GG
FauI 120 GCGGG CCCGC(4/6)
HapII 186 CCGG C^CGG
HpaII 186 CCGG C^CGG
MaeI 34 CTAG C^TAG
MboI 508 GATC ^GATC
MboII 72 GAAGA GAAGA(8/7)
MseI 242 TTAA T^TAA
MspI 186 CCGG C^CGG
MwoI 536 GCCAAGGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 185 CCCGG CC^[GC]GG
NdeII 508 GATC ^GATC
PaeR7I 150 CTCGAG C^TCGAG
PleI 153 GAGTC GAGTC(4/5)
PstI 59 CTGCAG CTGCA^G
RsaI 423 GTAC GT^AC
Sau3AI 508 GATC ^GATC
SfaNI 78 GATGC GCATC(5/9)
SfcI 59 CTGCAG C^T[GA][CT]AG
SmlI 150 CTCGAG C^T[CT][GA]AG
StyI 537 CCAAGG C^C[AT][AT]GG
TfiI 288 GATTC G^A[AT]TC
Tru9I 242 TTAA T^TAA
TseI 61 GCAGC G^C[AT]GC
Tsp45I 161 GTCAC ^GT[GC]AC
Tsp509I 298 AATT ^AATT
XhoI 150 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AciI 192 CCGC CCGC(-3/-1)
120 GCGG
AspHI 147 GTGCTC G[AT]GC[AT]^C
448 GTGCAC
BmyI 147 GTGCTC G[AGT]GC[ACT]^C
448 GTGCAC
BsiHKAI 147 GTGCTC G[AT]GC[AT]^C
448 GTGCAC
Bsp1286I 147 GTGCTC G[AGT]GC[ACT]^C
448 GTGCAC
BspMI 37 GCAGGT ACCTGC(4/8)
124 GCAGGT
BstNI 18 CCTGG CC^[AT]GG
429 CCTGG
EcoRII 18 CCTGG ^CC[AT]GG
429 CCTGG
Fnu4HI 61 GCAGC GC^[ACGT]GC
191 GCCGC
HinfI 153 GAGTC G^A[ACGT]TC
288 GATTC
ItaI 61 GCAGC GC^[ACGT]GC
191 GCCGC
MaeIII 161 GTCAC ^GT[ACGT]AC
338 GTTAC
MvaI 18 CCTGG CC^[AT]GG
429 CCTGG
StuI 30 AGGCCT AGG^CCT
218 AGGCCT
TaqI 151 TCGA T^CGA
510 TCGA
TthHB8I 151 TCGA T^CGA
510 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
BsrI BssKI BstF5I Cac8I DdeI
FokI HaeIII HphI MnlI MslI
NlaIV ScrFI TspRI