################################## # NO CUT ENZYMES ( 59 enzymes) # ################################## AatII AccI AccIII AclI Aor51HI AscI AseI AsnI AviII BbrPI BsaAI BseAI BsiEI BsiWI BspEI BssHII Bst1107I BstEII BstPI BstZ17I CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI KspI MluI MroI NaeI NgoMIV NheI NotI NruI PacI PaeR7I PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SnaBI Sse8387I SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 3285 GGTACC G^GTACC AgeI 3145 ACCGGT A^CCGGT AhdI 3244 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1817 GGGCCC GGGCC^C Asp718I 3285 GGTACC G^GTACC AspEI 3244 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3706 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 636 CCTAGG C^CTAGG BbvCI 5108 CCTCAGC CCTCAGC(-5/-2) BglI 1075 GCCTTCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 636 CCTAGG C^CTAGG BsmBI 1984 CGTCTC CGTCTC(1/5) BsmI 3951 GAATGC GAATGC(1/-1) BspDI 5044 ATCGAT AT^CGAT BspMI 3568 ACCTGC ACCTGC(4/8) BsrFI 3145 ACCGGT [GA]^CCGG[CT] BssSI 5032 CACGAG CACGAG(-5/-1) BstBI 830 TTCGAA TT^CGAA Cfr10I 3145 ACCGGT [GA]^CCGG[CT] ClaI 5044 ATCGAT AT^CGAT DraI 1294 TTTAAA TTT^AAA Eam1105I 3244 GACCACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 3412 GATATC GAT^ATC EcoT22I 2755 ATGCAT ATGCA^T HindIII 4132 AAGCTT A^AGCTT HpaI 4036 GTTAAC GTT^AAC KpnI 3285 GGTACC GGTAC^C NsiI 2755 ATGCAT ATGCA^T NspV 830 TTCGAA TT^CGAA PflFI 3706 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 3145 ACCGGT A^CCGGT SexAI 4673 ACCTGGT A^CC[AT]GGT SfuI 830 TTCGAA TT^CGAA SgrAI 3144 CACCGGTG C[GA]^CCGG[CT]G SmaI 1728 CCCGGG CCC^GGG SphI 950 GCATGC GCATG^C Tth111I 3706 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1728 CCCGGG C^CCGGG XmaI 1728 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AflII 1315 CTTAAG C^TTAAG 3909 CTTAAG Alw44I 417 GTGCAC G^TGCAC 3043 GTGCAC AocI 3451 CCTGAGG CC^T[ACGT]AGG 3882 CCTTAGG ApaLI 417 GTGCAC G^TGCAC 3043 GTGCAC AvaI 1534 CCCGAG C^[CT]CG[GA]G 1728 CCCGGG BcgI 2791 CGAGTGTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5385 CGACCCCGATGC BciVI 1006 GGATAC GTATCC(6/5) 4204 GGATAC BclI 407 TGATCA T^GATCA 4889 TGATCA BfrI 1315 CTTAAG C^TTAAG 3909 CTTAAG BglII 2993 AGATCT A^GATCT 5369 AGATCT BlpI 1641 GCTCAGC GC^T[ACGT]AGC 3627 GCTGAGC Bpu1102I 1641 GCTCAGC GC^T[ACGT]AGC 3627 GCTGAGC BsaWI 3145 ACCGGT [AT]^CCGG[AT] 3161 ACCGGA BsoBI 1534 CCCGAG C^[CT]CG[GA]G 1728 CCCGGG Bsu36I 3451 CCTGAGG CC^T[ACGT]AGG 3882 CCTTAGG CelII 1641 GCTCAGC GC^T[ACGT]AGC 3627 GCTGAGC Eco81I 3451 CCTGAGG CC^T[ACGT]AGG 3882 CCTTAGG EcoNI 1989 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 5166 CCTAGATAAGG FbaI 407 TGATCA T^GATCA 4889 TGATCA MfeI 716 CAATTG C^AATTG 1129 CAATTG MunI 716 CAATTG C^AATTG 1129 CAATTG NdeI 2752 CATATG CA^TATG 5021 CATATG ScaI 4460 AGTACT AGT^ACT 5411 AGTACT SpeI 519 ACTAGT A^CTAGT 2121 ACTAGT XcmI 4472 CCATGAAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5555 CCAAGCGGGTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BalI BamHI BanI BanII BbeI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI