(Linear) (Six-base) MAPSORT of: KIEE2318.seq Check: 3940 from: 1 to: 5940 KIEE2318.orf With 182 enzymes: SgfI * June 13, 2011 11:57 .. Acc65I G'GTAC_C Cuts at: 0 3285 5940 Size: 3285 2655 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 294 730 1343 1532 1680 2425 2922 3377 Size: 294 436 613 189 148 745 497 455 Cuts at: 3377 3416 5289 5940 Size: 39 1873 651 Fragments arranged by size: 1873 745 651 613 497 455 436 294 189 148 39 AflII C'TTAA_G Cuts at: 0 1315 3909 5940 Size: 1315 2594 2031 Fragments arranged by size: 2594 2031 1315 AflIII A'CryG_T Cuts at: 0 488 2346 3248 4316 5051 5276 5940 Size: 488 1858 902 1068 735 225 664 Fragments arranged by size: 1858 1068 902 735 664 488 225 AgeI A'CCGG_T Cuts at: 0 3145 5940 Size: 3145 2795 AhdI GACnn_n'nnGTC Cuts at: 0 3249 5940 Size: 3249 2691 AleI CACnn'nnGTG Cuts at: 0 3146 5940 Size: 3146 2794 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 475 507 5599 5631 5940 Size: 475 32 5092 32 309 Fragments arranged by size: 5092 475 309 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 31 946 1369 1638 5940 Size: 31 915 423 269 4302 Fragments arranged by size: 4302 915 423 269 31 ApaI G_GGCC'C Cuts at: 0 1821 5940 Size: 1821 4119 ApaLI G'TGCA_C Cuts at: 0 417 3043 5940 Size: 417 2626 2897 Fragments arranged by size: 2897 2626 417 ApoI r'AATT_y Cuts at: 0 380 930 1291 1615 2173 2381 2607 5940 Size: 380 550 361 324 558 208 226 3333 Fragments arranged by size: 3333 558 550 380 361 324 226 208 AvaI C'yCGr_G Cuts at: 0 1534 1728 5940 Size: 1534 194 4212 Fragments arranged by size: 4212 1534 194 AvrII C'CTAG_G Cuts at: 0 636 5940 Size: 636 5304 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 999 1032 5940 Size: 999 33 4908 Fragments arranged by size: 4908 999 33 BamHI G'GATC_C Cuts at: 0 1530 2421 3351 3465 3554 5940 Size: 1530 891 930 114 89 2386 Fragments arranged by size: 2386 1530 930 891 114 89 BanI G'GyrC_C Cuts at: 0 724 886 2617 3285 3637 3868 3964 4897 Size: 724 162 1731 668 352 231 96 933 Cuts at: 4897 5940 Size: 1043 Fragments arranged by size: 1731 1043 933 724 668 352 231 162 96 BanII G_rGCy'C Cuts at: 0 1417 1429 1735 1821 2935 4489 5486 5940 Size: 1417 12 306 86 1114 1554 997 454 Fragments arranged by size: 1554 1417 1114 997 454 306 86 12 BbeI G_GCGC'C Cuts at: 0 3641 3968 4901 5940 Size: 3641 327 933 1039 Fragments arranged by size: 3641 1039 933 327 BbsI GAAGACnn'nnnn_ Cuts at: 0 718 2544 2649 3705 5940 Size: 718 1826 105 1056 2235 Fragments arranged by size: 2235 1826 1056 718 105 BbvCI CC'TCA_GC Cuts at: 0 5109 5940 Size: 5109 831 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2780 2814 5374 5408 5940 Size: 2780 34 2560 34 532 Fragments arranged by size: 2780 2560 532 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1000 4198 5940 Size: 1000 3198 1742 Fragments arranged by size: 3198 1742 1000 BclI T'GATC_A Cuts at: 0 407 4889 5940 Size: 407 4482 1051 Fragments arranged by size: 4482 1051 407 BfrBI ATG'CAT Cuts at: 0 2757 5940 Size: 2757 3183 BglI GCCn_nnn'nGGC Cuts at: 0 1081 5940 Size: 1081 4859 BglII A'GATC_T Cuts at: 0 2993 5369 5940 Size: 2993 2376 571 Fragments arranged by size: 2993 2376 571 BlpI GC'TnA_GC Cuts at: 0 1642 3628 5940 Size: 1642 1986 2312 Fragments arranged by size: 2312 1986 1642 Bme1580I G_kGCm'C Cuts at: 0 421 729 1821 2158 2622 3047 5465 5940 Size: 421 308 1092 337 464 425 2418 475 Fragments arranged by size: 2418 1092 475 464 425 421 337 308 BmgBI CAC'GTC Cuts at: 0 2508 5940 Size: 2508 3432 BmrI ACTGGGnnnn_n' Cuts at: 0 5394 5940 Size: 5394 546 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 1285 2974 3754 5317 5794 5940 Size: 262 1023 1689 780 1563 477 146 Fragments arranged by size: 1689 1563 1023 780 477 262 146 Bpu10I CC'TnA_GC Cuts at: 0 2929 5109 5940 Size: 2929 2180 831 Fragments arranged by size: 2929 2180 831 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 85 3730 5940 Size: 85 3645 2210 Fragments arranged by size: 3645 2210 85 BsaI GGTCTCn'nnnn_ Cuts at: 0 1718 2809 3595 3897 5350 5940 Size: 1718 1091 786 302 1453 590 Fragments arranged by size: 1718 1453 1091 786 590 302 BsaBI GATnn'nnATC Cuts at: 0 3007 4249 4591 5940 Size: 3007 1242 342 1349 Fragments arranged by size: 3007 1349 1242 342 BsaHI Gr'CG_yC Cuts at: 0 1983 3638 3965 4898 5940 Size: 1983 1655 327 933 1042 Fragments arranged by size: 1983 1655 1042 933 327 BsaWI w'CCGG_w Cuts at: 0 3145 3161 5940 Size: 3145 16 2779 Fragments arranged by size: 3145 2779 16 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 475 505 1717 1747 3373 3403 5940 Size: 475 30 1212 30 1626 30 2537 Fragments arranged by size: 2537 1626 1212 475 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1282 1534 1789 1866 3381 5698 5940 Size: 1282 252 255 77 1515 2317 242 Fragments arranged by size: 2317 1515 1282 255 252 242 77 BseYI C'CCAG_C Cuts at: 0 988 1070 1891 4482 5940 Size: 988 82 821 2591 1458 Fragments arranged by size: 2591 1458 988 821 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2963 3170 5348 5940 Size: 2963 207 2178 592 Fragments arranged by size: 2963 2178 592 207 BsiHKAI G_wGCw'C Cuts at: 0 421 1429 1763 2321 2935 3047 3590 4712 Size: 421 1008 334 558 614 112 543 1122 Cuts at: 4712 5486 5812 5940 Size: 774 326 128 Fragments arranged by size: 1122 1008 774 614 558 543 421 334 326 128 112 BsmI GAATG_Cn' Cuts at: 0 3957 5940 Size: 3957 1983 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1990 5940 Size: 1990 3950 Bsp1286I G_dGCh'C Cuts at: 0 421 729 1417 1429 1735 1763 1821 2158 Size: 421 308 688 12 306 28 58 337 Cuts at: 2158 2321 2622 2935 3047 3590 4489 4712 5465 Size: 163 301 313 112 543 899 223 753 Cuts at: 5465 5486 5812 5940 Size: 21 326 128 Fragments arranged by size: 899 753 688 543 421 337 326 313 308 306 301 223 163 128 112 58 28 21 12 BspHI T'CATG_A Cuts at: 0 443 605 1625 3617 3980 4439 4583 5940 Size: 443 162 1020 1992 363 459 144 1357 Fragments arranged by size: 1992 1357 1020 459 443 363 162 144 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3577 5940 Size: 3577 2363 BsrBI CCG'CTC Cuts at: 0 1874 5513 5613 5940 Size: 1874 3639 100 327 Fragments arranged by size: 3639 1874 327 100 BsrDI GCAATG_nn' Cuts at: 0 83 530 648 1000 1307 2318 3195 3290 Size: 83 447 118 352 307 1011 877 95 Cuts at: 3290 5282 5940 Size: 1992 658 Fragments arranged by size: 1992 1011 877 658 447 352 307 118 95 83 BsrFI r'CCGG_y Cuts at: 0 3145 5940 Size: 3145 2795 BsrGI T'GTAC_A Cuts at: 0 335 4319 5279 5940 Size: 335 3984 960 661 Fragments arranged by size: 3984 960 661 335 BssSI C'ACGA_G Cuts at: 0 5032 5940 Size: 5032 908 BstAPI GCAn_nnn'nTGC Cuts at: 0 1342 5087 5431 5528 5940 Size: 1342 3745 344 97 412 Fragments arranged by size: 3745 1342 412 344 97 BstBI TT'CG_AA Cuts at: 0 831 5940 Size: 831 5109 BstXI CCAn_nnnn'nTGG Cuts at: 0 584 3166 3184 3297 5295 5940 Size: 584 2582 18 113 1998 645 Fragments arranged by size: 2582 1998 645 584 113 18 BstYI r'GATC_y Cuts at: 0 1530 2035 2421 2993 3351 3465 3554 5369 Size: 1530 505 386 572 358 114 89 1815 Cuts at: 5369 5496 5940 Size: 127 444 Fragments arranged by size: 1815 1530 572 505 444 386 358 127 114 89 Bsu36I CC'TnA_GG Cuts at: 0 3452 3883 5940 Size: 3452 431 2057 Fragments arranged by size: 3452 2057 431 BtgI C'CryG_G Cuts at: 0 577 1232 2051 2417 2669 5940 Size: 577 655 819 366 252 3271 Fragments arranged by size: 3271 819 655 577 366 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1363 5472 5940 Size: 1363 4109 468 Fragments arranged by size: 4109 1363 468 BtsI GCAGTG_nn' Cuts at: 0 1636 3079 3639 5526 5665 5702 5940 Size: 1636 1443 560 1887 139 37 238 Fragments arranged by size: 1887 1636 1443 560 238 139 37 ClaI AT'CG_AT Cuts at: 0 5045 5940 Size: 5045 895 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1991 2026 4601 4636 5940 Size: 1991 35 2575 35 1304 Fragments arranged by size: 2575 1991 1304 35 35 DraI TTT'AAA Cuts at: 0 1296 5940 Size: 1296 4644 EaeI y'GGCC_r Cuts at: 0 1229 1352 2561 2645 5940 Size: 1229 123 1209 84 3295 Fragments arranged by size: 3295 1229 1209 123 84 EarI CTCTTCn'nnn_ Cuts at: 0 181 313 902 1325 1434 1483 1807 2132 Size: 181 132 589 423 109 49 324 325 Cuts at: 2132 3425 4978 5603 5940 Size: 1293 1553 625 337 Fragments arranged by size: 1553 1293 625 589 423 337 325 324 181 132 109 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2091 5940 Size: 2091 3849 EcoICRI GAG'CTC Cuts at: 0 1427 2933 5484 5940 Size: 1427 1506 2551 456 Fragments arranged by size: 2551 1506 1427 456 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 262 294 730 1285 1343 1532 1680 2425 Size: 262 32 436 555 58 189 148 745 Cuts at: 2425 2922 2974 3377 3416 3754 5289 5317 5794 Size: 497 52 403 39 338 1535 28 477 Cuts at: 5794 5940 Size: 146 Fragments arranged by size: 1535 745 555 497 477 436 403 338 262 189 148 146 58 52 39 32 28 EcoNI CCTnn'n_nnAGG Cuts at: 0 1993 5170 5940 Size: 1993 3177 770 Fragments arranged by size: 3177 1993 770 EcoO109I rG'GnC_Cy Cuts at: 0 28 573 1708 2029 3435 3887 5562 5940 Size: 28 545 1135 321 1406 452 1675 378 Fragments arranged by size: 1675 1406 1135 545 452 378 321 28 EcoRV GAT'ATC Cuts at: 0 3414 5940 Size: 3414 2526 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 4200 5940 Size: 4200 1740 HaeII r_GCGC'y Cuts at: 0 1830 3641 3968 4901 5940 Size: 1830 1811 327 933 1039 Fragments arranged by size: 1830 1811 1039 933 327 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1717 1749 2456 2488 3235 3267 3373 3405 Size: 1717 32 707 32 747 32 106 32 Cuts at: 3405 4617 4649 5502 5534 5674 5706 5940 Size: 1212 32 853 32 140 32 234 Fragments arranged by size: 1717 1212 853 747 707 234 140 106 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 3522 4038 4110 5940 Size: 3522 516 72 1830 Fragments arranged by size: 3522 1830 516 72 HindIII A'AGCT_T Cuts at: 0 4132 5940 Size: 4132 1808 HpaI GTT'AAC Cuts at: 0 4038 5940 Size: 4038 1902 KasI G'GCGC_C Cuts at: 0 3637 3964 4897 5940 Size: 3637 327 933 1043 Fragments arranged by size: 3637 1043 933 327 KpnI G_GTAC'C Cuts at: 0 3289 5940 Size: 3289 2651 MfeI C'AATT_G Cuts at: 0 716 1129 5940 Size: 716 413 4811 Fragments arranged by size: 4811 716 413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1286 2428 3329 5564 5940 Size: 1286 1142 901 2235 376 Fragments arranged by size: 2235 1286 1142 901 376 MscI TGG'CCA Cuts at: 0 1231 1354 2563 2647 5940 Size: 1231 123 1209 84 3293 Fragments arranged by size: 3293 1231 1209 123 84 MslI CAynn'nnrTG Cuts at: 0 607 1186 2416 2756 2836 3146 3164 3295 Size: 607 579 1230 340 80 310 18 131 Cuts at: 3295 3616 3985 4438 5491 5940 Size: 321 369 453 1053 449 Fragments arranged by size: 1230 1053 607 579 453 449 369 340 321 310 131 80 18 MspA1I CmG'CkG Cuts at: 0 813 1844 1853 2392 3203 3501 4264 5113 Size: 813 1031 9 539 811 298 763 849 Cuts at: 5113 5877 5940 Size: 764 63 Fragments arranged by size: 1031 849 813 811 764 763 539 298 63 9 NarI GG'CG_CC Cuts at: 0 3638 3965 4898 5940 Size: 3638 327 933 1042 Fragments arranged by size: 3638 1042 933 327 NcoI C'CATG_G Cuts at: 0 577 1232 2051 2417 5940 Size: 577 655 819 366 3523 Fragments arranged by size: 3523 819 655 577 366 NdeI CA'TA_TG Cuts at: 0 2753 5022 5940 Size: 2753 2269 918 Fragments arranged by size: 2753 2269 918 NsiI A_TGCA'T Cuts at: 0 2759 5940 Size: 2759 3181 NspI r_CATG'y Cuts at: 0 954 2350 2589 3252 4320 5055 5280 5940 Size: 954 1396 239 663 1068 735 225 660 Fragments arranged by size: 1396 1068 954 735 663 660 239 225 PasI CC'CwG_GG Cuts at: 0 2622 3849 5182 5940 Size: 2622 1227 1333 758 Fragments arranged by size: 2622 1333 1227 758 PciI A'CATG_T Cuts at: 0 2346 3248 4316 5051 5276 5940 Size: 2346 902 1068 735 225 664 Fragments arranged by size: 2346 1068 902 735 664 225 PflMI CCAn_nnn'nTGG Cuts at: 0 225 2570 3788 4751 5188 5532 5940 Size: 225 2345 1218 963 437 344 408 Fragments arranged by size: 2345 1218 963 437 408 344 225 PfoI T'CCnGG_A Cuts at: 0 3692 3731 4273 5294 5940 Size: 3692 39 542 1021 646 Fragments arranged by size: 3692 1021 646 542 39 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 475 507 950 982 1729 1761 5940 Size: 475 32 443 32 747 32 4179 Fragments arranged by size: 4179 747 475 443 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 573 1708 3435 3887 5562 5940 Size: 573 1135 1727 452 1675 378 Fragments arranged by size: 1727 1675 1135 573 452 378 PspOMI G'GGCC_C Cuts at: 0 1817 5940 Size: 1817 4123 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 4645 4677 5940 Size: 4645 32 1263 Fragments arranged by size: 4645 1263 32 PstI C_TGCA'G Cuts at: 0 812 5164 5697 5708 5940 Size: 812 4352 533 11 232 Fragments arranged by size: 4352 812 533 232 11 PvuII CAG'CTG Cuts at: 0 813 4264 5113 5940 Size: 813 3451 849 827 Fragments arranged by size: 3451 849 827 813 SacI G_AGCT'C Cuts at: 0 1429 2935 5486 5940 Size: 1429 1506 2551 454 Fragments arranged by size: 2551 1506 1429 454 SanDI GG'GwC_CC Cuts at: 0 573 5940 Size: 573 5367 SapI GCTCTTCn'nnn_ Cuts at: 0 1434 4978 5603 5940 Size: 1434 3544 625 337 Fragments arranged by size: 3544 1434 625 337 ScaI AGT'ACT Cuts at: 0 4462 5413 5940 Size: 4462 951 527 Fragments arranged by size: 4462 951 527 SexAI A'CCwGG_T Cuts at: 0 4673 5940 Size: 4673 1267 SfcI C'TryA_G Cuts at: 0 808 1839 1848 1857 2357 4265 5160 5693 Size: 808 1031 9 9 500 1908 895 533 Cuts at: 5693 5704 5819 5940 Size: 11 115 121 Fragments arranged by size: 1908 1031 895 808 533 500 121 115 11 9 9 SfoI GGC'GCC Cuts at: 0 3639 3966 4899 5940 Size: 3639 327 933 1041 Fragments arranged by size: 3639 1041 933 327 SgrAI Cr'CCGG_yG Cuts at: 0 3145 5940 Size: 3145 2795 SmaI CCC'GGG Cuts at: 0 1730 5940 Size: 1730 4210 SmlI C'TyrA_G Cuts at: 0 100 1315 3745 3909 5940 Size: 100 1215 2430 164 2031 Fragments arranged by size: 2430 2031 1215 164 100 SpeI A'CTAG_T Cuts at: 0 519 2121 5940 Size: 519 1602 3819 Fragments arranged by size: 3819 1602 519 SphI G_CATG'C Cuts at: 0 954 5940 Size: 954 4986 StuI AGG'CCT Cuts at: 0 224 707 1582 5940 Size: 224 483 875 4358 Fragments arranged by size: 4358 875 483 224 StyI C'CwwG_G Cuts at: 0 219 577 636 1232 2051 2417 3956 4533 Size: 219 358 59 596 819 366 1539 577 Cuts at: 4533 5441 5940 Size: 908 499 Fragments arranged by size: 1539 908 819 596 577 499 366 358 219 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 712 2605 4459 5195 5413 5940 Size: 712 1893 1854 736 218 527 Fragments arranged by size: 1893 1854 736 712 527 218 TatI w'GTAC_w Cuts at: 0 335 485 3227 4319 4460 5279 5411 5940 Size: 335 150 2742 1092 141 819 132 529 Fragments arranged by size: 2742 1092 819 529 335 150 141 132 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2597 2629 5940 Size: 2597 32 3311 Fragments arranged by size: 3311 2597 32 Tth111I GACn'n_nGTC Cuts at: 0 3709 5940 Size: 3709 2231 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 4479 5562 5940 Size: 4479 1083 378 Fragments arranged by size: 4479 1083 378 XmaI C'CCGG_G Cuts at: 0 1728 5940 Size: 1728 4212 XmnI GAAnn'nnTTC Cuts at: 0 1744 4372 4417 4759 5940 Size: 1744 2628 45 342 1181 Fragments arranged by size: 2628 1744 1181 342 45 Enzymes that do cut: Acc65I AcuI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI PspOMI PsrI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AlfI AscI AseI AsiSI BmtI BplI BsaAI BsiEI BsiWI BspEI BssHII BstEII BstZ17I DraIII DrdI EagI EcoRI FseI FspI FspAI MluI NaeI NgoMIV NheI NotI NruI PacI PmeI PmlI PshAI PsiI PspXI PvuI RsrII SacII SalI SbfI SfiI SgfI SnaBI SrfI SspI SwaI XbaI XhoI ZraI