Restriction map for
pFN21AE6361
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BciVI BclI BcnI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PflFI PinAI PleI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI StuI
StyI SwaI Tth111I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AflII 205 CTTAAG C^TTAAG
AseI 813 ATTAAT AT^TAAT
AsnI 813 ATTAAT AT^TAAT
AspHI 42 GAGCTC G[AT]GC[AT]^C
AvaI 698 CTCGAG C^[CT]CG[GA]G
BalI 996 TGGCCA TGG^CCA
BanI 241 GGCACC G^G[CT][GA]CC
BbvCI 130 GCTGAGG CCTCAGC(-5/-2)
BbvI 358 GCTGC GCAGC(8/12)
BcgI 219 GCAAGGGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 205 CTTAAG C^TTAAG
BsaAI 992 TACGTG [CT]AC^GT[GA]
BsaI 224 GGTCTC GGTCTC(1/5)
BsaJI 268 CCGTGG C^C[ACGT][ACGT]GG
BseRI 720 GAGGAG GAGGAG(10/8)
BsiHKAI 42 GAGCTC G[AT]GC[AT]^C
BsmAI 225 GTCTC GTCTC(1/5)
BsmFI 807 GGGAC GGGAC(10/14)
BsoBI 698 CTCGAG C^[CT]CG[GA]G
BspMI 760 GCAGGT ACCTGC(4/8)
BsrFI 108 ACCGGC [GA]^CCGG[CT]
BsrI 863 CCAGT ACTGG(1/-1)
BstYI 1001 AGATCC [GA]^GATC[CT]
Cfr10I 108 ACCGGC [GA]^CCGG[CT]
DraII 347 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 609 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 268 CCGTGG C^C[GA][CT]GG
EarI 887 GAAGAG CTCTTC(1/4)
EcoNI 74 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 347 GGGTCCT [GA]G^G[ACGT]CC[CT]
HaeII 24 GGCGCT [GA]GCGC^[CT]
HinfI 496 GAATC G^A[ACGT]TC
Ksp632I 887 GAAGAG CTCTTC(1/4)
MaeII 993 ACGT A^CGT
MflI 1001 AGATCC [GA]^GATC[CT]
MluNI 996 TGGCCA TGG^CCA
MscI 996 TGGCCA TGG^CCA
NheI 379 GCTAGC G^CTAGC
NspI 177 GCATGC [GA]CATG^[CT]
PaeR7I 698 CTCGAG C^TCGAG
PflMI 591 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 347 GGGTCCT [GA]G^G[AT]CC[CT]
PshBI 813 ATTAAT AT^TAAT
PstI 283 CTGCAG CTGCA^G
SacI 42 GAGCTC GAGCT^C
SfaNI 676 GATGC GCATC(5/9)
SfcI 283 CTGCAG C^T[GA][CT]AG
SphI 177 GCATGC GCATG^C
TfiI 496 GAATC G^A[AT]TC
TseI 358 GCTGC G^C[AT]GC
Tsp45I 797 GTGAC ^GT[GC]AC
Van91I 591 CCAAATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 698 CTCGAG C^TCGAG
XhoII 1001 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 385 GATCC GGATC(4/5)
1002 GATCC
AvaII 348 GGTCC G^G[AT]CC
808 GGACC
BanII 10 GGGCTC G[GA]GC[CT]^C
42 GAGCTC
BsgI 366 CTGCAC GTGCAG(16/14)
758 GTGCAG
BssKI 597 CCTGG ^CC[ACGT]GG
898 CCTGG
BstNI 597 CCTGG CC^[AT]GG
898 CCTGG
DpnII 385 GATC ^GATC
1002 GATC
EcoRII 597 CCTGG ^CC[AT]GG
898 CCTGG
HgaI 376 GACGC GACGC(5/10)
431 GACGC
MboI 385 GATC ^GATC
1002 GATC
MspA1I 356 CAGCTG C[AC]G^C[GT]G
401 CAGCTG
MvaI 597 CCTGG CC^[AT]GG
898 CCTGG
NdeII 385 GATC ^GATC
1002 GATC
PvuII 356 CAGCTG CAG^CTG
401 CAGCTG
Sau3AI 385 GATC ^GATC
1002 GATC
ScrFI 597 CCTGG CC^[ACGT]GG
898 CCTGG
SspI 664 AATATT AAT^ATT
985 AATATT
TaqI 699 TCGA T^CGA
885 TCGA
TthHB8I 699 TCGA T^CGA
885 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BfaI BmyI BsiYI
BslI Bsp1286I BstF5I Cac8I CfoI
Cfr13I DdeI EaeI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeI MaeIII MboII
MnlI MseI MslI MspI MwoI
NlaIII NlaIV Sau96I SmlI Tru9I
Tsp509I TspRI