(Circular) (Six-base) MAPSORT of: pFN21AE6361.seq Check: 3606 from: 1 to: 5749 pFN21AE6361 5749 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2756 2756 Size: 5749 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3092 1937 Size: 1155 4594 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3812 5196 5464 1632 Size: 2180 1384 268 1917 Fragments arranged by size: 2180 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2210 820 Size: 197 1193 4359 Fragments arranged by size: 4359 1193 197 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 3492 5064 3492 Size: 1572 4177 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AseI AT'TA_AT Cuts at: 160 1032 2819 4303 160 Size: 872 1787 1484 1606 Fragments arranged by size: 1787 1606 1484 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 2703 3074 1870 Size: 73 760 371 4545 Fragments arranged by size: 4545 760 371 73 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2246 3070 4525 618 Size: 325 217 627 459 824 1455 1842 Fragments arranged by size: 1842 1455 824 627 459 325 217 BanII G_rGCy'C Cuts at: 1391 2019 2051 3068 1391 Size: 628 32 1017 4072 Fragments arranged by size: 4072 1017 628 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4778 BbvCI CC'TCA_GC Cuts at: 2136 2136 Size: 5749 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2213 2247 3940 3974 1903 Size: 34 50 34 192 34 1693 34 3678 Fragments arranged by size: 3678 1693 192 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4360 136 Size: 107 122 71 631 605 2688 1525 Fragments arranged by size: 2688 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5744 5744 Size: 5749 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 2249 3810 4966 1790 Size: 459 1561 1156 2573 Fragments arranged by size: 2573 1561 1156 459 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3480 4438 442 Size: 416 53 83 399 24 2063 958 1753 Fragments arranged by size: 2063 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2388 1056 Size: 1332 4417 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4409 1239 Size: 3170 2579 Bpu10I CC'TnA_GC Cuts at: 2021 2136 2021 Size: 115 5634 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2955 3874 4739 5037 2955 Size: 919 865 298 3667 Fragments arranged by size: 3667 919 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2235 4412 882 Size: 617 736 2177 2219 Fragments arranged by size: 2219 2177 736 617 BsaAI yAC'GTr Cuts at: 493 1204 2999 3540 493 Size: 711 1795 541 2702 Fragments arranged by size: 2702 1795 711 541 BsaBI GATnn'nnATC Cuts at: 5743 5743 Size: 5749 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3556 3938 4573 275 Size: 53 83 186 936 2023 382 635 1451 Fragments arranged by size: 2023 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5524 Fragments arranged by size: 5524 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2740 1186 Size: 1554 4195 BseYI C'CCAG_C Cuts at: 2011 2127 2634 4952 5448 2011 Size: 116 507 2318 496 2312 Fragments arranged by size: 2318 2312 507 496 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2356 2784 1163 Size: 573 620 428 4128 Fragments arranged by size: 4128 620 573 428 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3199 3960 4988 1540 Size: 275 186 1198 761 1028 2301 Fragments arranged by size: 2301 1198 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2051 3810 3895 4966 2051 Size: 1759 85 1071 2834 Fragments arranged by size: 2834 1759 1071 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2051 2249 3068 3810 3895 4966 Size: 399 229 32 198 819 742 85 1071 Cuts at: 4966 1391 Size: 2174 Fragments arranged by size: 2174 1071 819 742 399 229 198 85 32 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 3638 5368 3638 Size: 1730 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2756 3104 844 Size: 1912 348 3489 Fragments arranged by size: 3489 1912 348 BsrBI CCG'CTC Cuts at: 1394 1823 3636 1394 Size: 429 1813 3507 Fragments arranged by size: 3507 1813 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4244 4418 65 Size: 1183 2996 174 1396 Fragments arranged by size: 2996 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2113 4393 4580 1700 Size: 110 303 2280 187 2869 Fragments arranged by size: 2869 2280 303 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5749 BstYI r'GATC_y Cuts at: 1972 3006 3079 3446 3831 3848 5300 5386 5398 Size: 1034 73 367 385 17 1452 86 12 Cuts at: 5398 5744 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1034 385 367 346 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 2273 513 Size: 552 1208 3989 Fragments arranged by size: 3989 1208 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 2368 2550 3265 4059 4079 2368 Size: 182 715 794 20 4038 Fragments arranged by size: 4038 794 715 182 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DraIII CAC_nnn'GTG Cuts at: 2619 2619 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2270 2302 3001 Size: 54 1425 50 275 18 440 32 699 Cuts at: 3001 3196 4085 5714 8 Size: 195 889 1629 43 Fragments arranged by size: 1629 1425 889 699 440 275 195 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2887 3679 1353 Size: 1534 792 3423 Fragments arranged by size: 3423 1534 792 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2179 4336 4710 4856 387 Size: 1064 138 590 2157 374 146 1280 Fragments arranged by size: 2157 1280 1064 590 374 146 138 EcoICRI GAG'CTC Cuts at: 2049 2049 Size: 5749 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3812 4409 5196 5464 1239 Size: 393 2180 597 787 268 1524 Fragments arranged by size: 2180 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2083 2083 Size: 5749 EcoO109I rG'GnC_Cy Cuts at: 2353 2353 Size: 5749 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2996 2996 Size: 5749 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 2033 4896 1678 Size: 355 2863 2531 Fragments arranged by size: 2863 2531 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2057 2089 4390 4422 4464 Size: 32 135 32 485 32 2301 32 42 Cuts at: 4464 4496 1373 Size: 32 2626 Fragments arranged by size: 2626 2301 485 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3093 3339 669 Size: 988 281 1155 246 3079 Fragments arranged by size: 3079 1155 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3339 3339 Size: 5749 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4863 5047 1305 Size: 3558 184 2007 Fragments arranged by size: 3558 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3003 10 Size: 54 1425 343 1171 2756 Fragments arranged by size: 2756 1425 1171 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2272 2322 2821 2883 3707 4066 4225 Size: 648 1106 50 499 62 824 359 159 Cuts at: 4225 518 Size: 2042 Fragments arranged by size: 2042 1106 824 648 499 359 159 62 50 MspA1I CmG'CkG Cuts at: 1606 2363 2408 3165 3842 4990 5235 1606 Size: 757 45 757 677 1148 245 2120 Fragments arranged by size: 2120 1148 757 757 677 245 45 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5197 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 2384 1052 Size: 1332 4417 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 2186 3107 4652 1132 Size: 97 957 921 1545 2229 Fragments arranged by size: 2229 1545 957 921 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5749 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 2602 1108 Size: 1494 4255 PfoI T'CCnGG_A Cuts at: 2601 2601 Size: 5749 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2353 2353 Size: 5749 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5749 PsiI TTA'TAA Cuts at: 2627 3319 2627 Size: 692 5057 PstI C_TGCA'G Cuts at: 830 2292 3101 830 Size: 1462 809 3478 Fragments arranged by size: 3478 1462 809 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2363 2408 2363 Size: 45 5704 SacI G_AGCT'C Cuts at: 2051 2051 Size: 5749 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SfcI C'TryA_G Cuts at: 826 1045 2288 3097 4232 4913 5104 826 Size: 219 1243 809 1135 681 191 1471 Fragments arranged by size: 1471 1243 1135 809 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 3076 1872 Size: 1204 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2210 2703 2970 3853 4754 5016 Size: 197 926 267 493 267 883 901 262 Cuts at: 5016 820 Size: 1553 Fragments arranged by size: 1553 926 901 883 493 267 267 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 2186 3107 1132 Size: 1054 921 3774 Fragments arranged by size: 3774 1054 921 SspI AAT'ATT Cuts at: 5 52 2671 2992 3673 5 Size: 47 2619 321 681 2081 Fragments arranged by size: 2619 2081 681 321 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5749 StyI C'CwwG_G Cuts at: 513 1065 3188 513 Size: 552 2123 3074 Fragments arranged by size: 3074 2123 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2266 3776 3793 3946 4131 4576 325 Size: 1255 686 1510 17 153 185 445 1498 Fragments arranged by size: 1510 1498 1255 686 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3995 5707 96 Size: 274 80 33 51 1443 2018 1712 138 Fragments arranged by size: 2018 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2322 2354 5709 5741 2322 Size: 32 3355 32 2330 Fragments arranged by size: 3355 2330 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XhoI C'TCGA_G Cuts at: 1943 2703 1943 Size: 760 4989 XmaI C'CCGG_G Cuts at: 1870 3074 1870 Size: 1204 4545 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI