################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbvCI BciVI BclI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeII MamI MfeI MluI MluNI MroI MscI MunI NcoI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SalI SapI SbfI SexAI SfcI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccI 694 GTAGAC GT^[AC][GT]AC AcyI 427 GGCGCC G[GA]^CG[CT]C AflIII 855 ACATGT A^C[GA][CT]GT Asp700I 1360 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 37 GAGCTC G[AT]GC[AT]^C AspI 240 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 427 GGCGCC G^G[CT][GA]CC BbeI 427 GGCGCC GGCGC^C BcgI 1225 CGAGCCATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 828 AGATCT A^GATCT BlpI 97 GCTGAGC GC^T[ACGT]AGC Bpu1102I 97 GCTGAGC GC^T[ACGT]AGC BsaHI 427 GGCGCC G[GA]^CG[CT]C BsaI 406 GAGACC GGTCTC(1/5) BsiHKAI 37 GAGCTC G[AT]GC[AT]^C BsmBI 238 GAGACG CGTCTC(1/5) BspLU11I 855 ACATGT A^CATGT BsrBI 411 CCGCTC CCGCTC(-3/-3) BsrFI 605 GCCGGC [GA]^CCGG[CT] BstYI 828 AGATCT [GA]^GATC[CT] CelII 97 GCTGAGC GC^T[ACGT]AGC Cfr10I 605 GCCGGC [GA]^CCGG[CT] DraII 467 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 962 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 467 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 482 CCAAGG C^C[AT][AT]GG HgaI 1163 GACGC GACGC(5/10) Hin1I 427 GGCGCC G[GA]^CG[CT]C KasI 427 GGCGCC G^GCGCC KspI 490 CCGCGG CCGC^GG MaeIII 942 GTTAC ^GT[ACGT]AC MflI 828 AGATCT [GA]^GATC[CT] NaeI 605 GCCGGC GCC^GGC NarI 427 GGCGCC GG^CGCC NgoMIV 605 GCCGGC G^CCGGC NheI 1032 GCTAGC G^CTAGC NspI 855 ACATGT [GA]CATG^[CT] PflFI 240 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 658 GACTC GAGTC(4/5) SacI 37 GAGCTC GAGCT^C SacII 490 CCGCGG CCGC^GG SfoI 427 GGCGCC GGC^GCC SmaI 72 CCCGGG CCC^GGG SspI 772 AATATT AAT^ATT StyI 482 CCAAGG C^C[AT][AT]GG Tth111I 240 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 828 AGATCT [GA]^GATC[CT] XmaCI 72 CCCGGG C^CCGGG XmaI 72 CCCGGG C^CCGGG XmnI 1360 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BbsI 289 GAAGAC GAAGAC(2/6) 1360 GAAGAC BpuAI 289 GAAGAC GAAGAC(2/6) 1360 GAAGAC BsrDI 730 GCAATG GCAATG(2/0) 1004 GCAATG DsaI 490 CCGCGG C^C[GA][CT]GG 1174 CCACGG MslI 15 CACCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 856 CATGTAGATG NdeI 952 CATATG CA^TATG 1230 CATATG ScaI 679 AGTACT AGT^ACT 1294 AGTACT TaqI 1193 TCGA T^CGA 1224 TCGA TfiI 1108 GAATC G^A[AT]TC 1321 GATTC TthHB8I 1193 TCGA T^CGA 1224 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AfaI AluI AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp509I TspRI