(Circular) (Six-base) MAPSORT of: pFN21AE2376.seq Check: 6852 from: 1 to: 6256 pFN21AE2376 6256 bp With 182 enzymes: SgfI * September 20, 2011 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4066 278 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2700 3599 1937 Size: 763 899 4594 Fragments arranged by size: 4594 899 763 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3046 3384 4319 5703 5971 1632 Size: 1414 338 935 1384 268 1917 Fragments arranged by size: 1917 1414 1384 935 338 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 2860 5155 6132 1203 Size: 1657 2295 977 1327 Fragments arranged by size: 2295 1657 1327 977 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 3999 5571 3999 Size: 1572 4684 ApaLI G'TGCA_C Cuts at: 4313 5469 4313 Size: 1156 5100 ApoI r'AATT_y Cuts at: 1493 3565 3791 3943 6052 1493 Size: 2072 226 152 2109 1697 Fragments arranged by size: 2109 2072 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4810 160 Size: 872 3778 1606 Fragments arranged by size: 3778 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2111 2264 3581 1870 Size: 73 134 34 153 1317 4545 Fragments arranged by size: 4545 1317 153 134 73 34 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2432 3577 5032 618 Size: 325 217 627 645 1145 1455 1842 Fragments arranged by size: 1842 1455 1145 645 627 325 217 BanII G_rGCy'C Cuts at: 1391 2046 2060 2109 2264 2429 2487 3575 1391 Size: 655 14 49 155 165 58 1088 4072 Fragments arranged by size: 4072 1088 655 165 155 58 49 14 BbeI G_GCGC'C Cuts at: 2436 2436 Size: 6256 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2301 3372 928 Size: 971 402 1071 3812 Fragments arranged by size: 3812 1071 971 402 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3219 3253 4447 4481 1903 Size: 34 1282 34 1194 34 3678 Fragments arranged by size: 3678 1282 1194 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4148 5358 4148 Size: 1210 5046 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2279 2382 2511 Size: 107 122 71 631 605 607 103 129 Cuts at: 2511 2616 4867 136 Size: 105 2251 1525 Fragments arranged by size: 2251 1525 631 607 605 129 122 107 105 103 71 BglII A'GATC_T Cuts at: 2833 6251 2833 Size: 3418 2838 BlpI GC'TnA_GC Cuts at: 2103 3673 2103 Size: 1570 4686 Bme1580I G_kGCm'C Cuts at: 1790 4317 5473 1790 Size: 2527 1156 2573 Fragments arranged by size: 2573 2527 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2586 3987 4945 Size: 416 53 83 399 24 1169 1401 958 Cuts at: 4945 442 Size: 1753 Fragments arranged by size: 1753 1401 1169 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3041 1056 Size: 1985 4271 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2062 2434 2062 Size: 372 5884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2498 2533 2715 4916 1239 Size: 818 441 35 182 2201 2579 Fragments arranged by size: 2579 2201 818 441 182 35 Bpu10I CC'TnA_GC Cuts at: 2054 2651 2054 Size: 597 5659 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4381 5246 5544 4381 Size: 865 298 5093 Fragments arranged by size: 5093 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2405 4919 882 Size: 617 906 2514 2219 Fragments arranged by size: 2514 2219 906 617 BsaAI yAC'GTr Cuts at: 493 1204 4047 493 Size: 711 2843 2702 Fragments arranged by size: 2843 2702 711 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2433 4063 4445 5080 Size: 53 83 186 936 900 1630 382 635 Cuts at: 5080 275 Size: 1451 Fragments arranged by size: 1630 1451 936 900 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4689 5087 5361 5508 5919 4689 Size: 398 274 147 411 5026 Fragments arranged by size: 5026 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2618 2648 1178 Size: 30 165 30 1215 30 4786 Fragments arranged by size: 4786 1215 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2129 2132 2135 2144 2147 2150 2159 Size: 868 75 3 3 9 3 3 9 Cuts at: 2159 2235 2315 2318 2330 2333 2369 2372 2495 Size: 76 80 3 12 3 36 3 123 Cuts at: 2495 2892 3067 1186 Size: 397 175 4375 Fragments arranged by size: 4375 868 397 175 123 80 76 75 36 12 9 9 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 5459 5955 5459 Size: 496 5760 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5683 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3706 4467 5495 1540 Size: 275 186 1705 761 1028 2301 Fragments arranged by size: 2301 1705 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 4317 4402 5473 2046 Size: 2271 85 1071 2829 Fragments arranged by size: 2829 2271 1071 85 BsmI GAATG_Cn' Cuts at: 3767 3860 3767 Size: 93 6163 BsmBI CGTCTCn'nnnn_ Cuts at: 2237 2237 Size: 6256 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2060 2109 2264 2429 2487 3575 Size: 399 256 14 49 155 165 58 1088 Cuts at: 3575 4317 4402 5473 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1088 1071 742 399 256 165 155 85 58 49 14 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3611 844 Size: 2767 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2418 4143 1394 Size: 429 595 1725 3507 Fragments arranged by size: 3507 1725 595 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2742 3016 4751 4925 65 Size: 1183 1494 274 1735 174 1396 Fragments arranged by size: 1735 1494 1396 1183 274 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2610 4900 5087 1700 Size: 110 800 2290 187 2869 Fragments arranged by size: 2869 2290 800 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6256 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 4316 5328 4316 Size: 1012 5244 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6256 BstYI r'GATC_y Cuts at: 1972 2833 3586 3953 4338 4355 5807 5893 5905 Size: 861 753 367 385 17 1452 86 12 Cuts at: 5905 6251 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 861 753 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2495 3179 513 Size: 552 939 491 684 3590 Fragments arranged by size: 3590 939 684 552 491 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5517 Fragments arranged by size: 5517 683 56 BtsI GCAGTG_nn' Cuts at: 3772 4566 4586 3772 Size: 794 20 5442 Fragments arranged by size: 5442 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 3560 3560 Size: 6256 DraIII CAC_nnn'GTG Cuts at: 2972 2972 Size: 6256 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2028 3182 Size: 54 1425 50 275 18 177 21 1154 Cuts at: 3182 3703 4592 6221 8 Size: 521 889 1629 43 Fragments arranged by size: 1629 1425 1154 889 521 275 177 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 2136 3494 3503 4186 1353 Size: 768 15 1358 9 683 3423 Fragments arranged by size: 3423 1358 768 683 15 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2165 2220 2232 2601 4843 5217 Size: 1064 138 576 55 12 369 2242 374 Cuts at: 5217 5363 387 Size: 146 1280 Fragments arranged by size: 2242 1280 1064 576 374 369 146 138 55 12 EcoICRI GAG'CTC Cuts at: 2044 2044 Size: 6256 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2498 2533 2715 3046 3384 4319 Size: 393 425 441 35 182 331 338 935 Cuts at: 4319 4916 5703 5971 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 935 787 597 441 425 393 338 331 268 182 35 EcoO109I rG'GnC_Cy Cuts at: 2473 2473 Size: 6256 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2778 2778 Size: 6256 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 2174 2274 2370 2436 5403 1678 Size: 496 100 96 66 2967 2531 Fragments arranged by size: 2967 2531 496 100 96 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2234 2266 2618 2650 3211 Size: 32 135 32 662 32 352 32 561 Cuts at: 3211 3243 4897 4929 4971 5003 1373 Size: 32 1654 32 42 32 2626 Fragments arranged by size: 2626 1654 662 561 352 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3600 3846 669 Size: 988 281 1662 246 3079 Fragments arranged by size: 3079 1662 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6256 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KasI G'GCGC_C Cuts at: 2432 2432 Size: 6256 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3855 3855 Size: 6256 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 2616 5370 5554 1305 Size: 927 384 2754 184 2007 Fragments arranged by size: 2754 2007 927 384 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4434 Fragments arranged by size: 4434 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 2865 4214 4573 4732 518 Size: 648 858 841 1349 359 159 2042 Fragments arranged by size: 2042 1349 858 841 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2193 2343 2391 2451 2497 2553 3672 4349 Size: 587 150 48 60 46 56 1119 677 Cuts at: 4349 5497 5742 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1119 677 587 245 150 60 56 48 46 NaeI GCC'GGC Cuts at: 2612 2612 Size: 6256 NarI GG'CG_CC Cuts at: 2433 2433 Size: 6256 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4765 Fragments arranged by size: 4765 939 552 NdeI CA'TA_TG Cuts at: 387 2958 3236 387 Size: 2571 278 3407 Fragments arranged by size: 3407 2571 278 NgoMIV G'CCGG_C Cuts at: 2610 2610 Size: 6256 NheI G'CTAG_C Cuts at: 1052 3037 1052 Size: 1985 4271 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 2864 3614 5159 1132 Size: 97 1635 750 1545 2229 Fragments arranged by size: 2229 1635 1545 750 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6256 PciI A'CATG_T Cuts at: 2860 5155 2860 Size: 2295 3961 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6256 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PstI C_TGCA'G Cuts at: 830 3608 830 Size: 2778 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 SacI G_AGCT'C Cuts at: 2046 2046 Size: 6256 SacII CC_GC'GG Cuts at: 2498 2498 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SbfI CC_TGCA'GG Cuts at: 3608 3608 Size: 6256 ScaI AGT'ACT Cuts at: 2686 3301 4504 6216 2686 Size: 615 1203 1712 2726 Fragments arranged by size: 2726 1712 1203 615 SfcI C'TryA_G Cuts at: 826 1045 3604 4739 5420 5611 826 Size: 219 2559 1135 681 191 1471 Fragments arranged by size: 2559 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2434 2434 Size: 6256 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 2079 3583 1872 Size: 207 1504 4545 Fragments arranged by size: 4545 1504 207 SmlI C'TyrA_G Cuts at: 820 1017 1943 4360 5261 5523 820 Size: 197 926 2417 901 262 1553 Fragments arranged by size: 2417 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 3614 1132 Size: 2482 3774 SspI AAT'ATT Cuts at: 5 52 2779 4180 5 Size: 47 2727 1401 2081 Fragments arranged by size: 2727 2081 1401 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6256 StyI C'CwwG_G Cuts at: 513 1065 2004 2487 3695 513 Size: 552 939 483 1208 3074 Fragments arranged by size: 3074 1208 939 552 483 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3376 4283 4300 4453 4638 5083 325 Size: 1255 1796 907 17 153 185 445 1498 Fragments arranged by size: 1796 1498 1255 907 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2684 3299 4502 Size: 274 80 33 51 1443 707 615 1203 Cuts at: 4502 6214 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1203 707 615 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2837 2869 6216 6248 2837 Size: 32 3347 32 2845 Fragments arranged by size: 3347 2845 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2248 1316 Size: 932 5324 XbaI T'CTAG_A Cuts at: 3592 3592 Size: 6256 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 2077 3581 1870 Size: 207 1504 4545 Fragments arranged by size: 4545 1504 207 XmnI GAAnn'nnTTC Cuts at: 3369 4385 3369 Size: 1016 5240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4064 276 Size: 53 83 186 936 2530 2468 Fragments arranged by size: 2530 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI