################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AclI AcsI AgeI Aor51HI ApoI AscI AseI AsnI AspI AvrII BbrPI BclI BlnI BsiWI BspHI BssSI Bst1107I BstBI BstZ17I CpoI DraI DraIII Eco47III EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HpaI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspI SwaI Tsp509I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### Acc65I 1510 GGTACC G^GTACC AflII 1606 CTTAAG C^TTAAG AhdI 2158 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 385 GTGCAC G^TGCAC ApaLI 385 GTGCAC G^TGCAC Asp700I 36 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1510 GGTACC G^GTACC AspEI 2158 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbsI 1618 GAAGAC GAAGAC(2/6) BcgI 2654 CGAGGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3568 GTATCC GTATCC(6/5) BfrI 1606 CTTAAG C^TTAAG BlpI 2763 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2763 GCTCAGC GC^T[ACGT]AGC BpuAI 1618 GAAGAC GAAGAC(2/6) BsaAI 1264 TACGTG [CT]AC^GT[GA] BsaBI 639 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1926 CGTCTC CGTCTC(1/5) Bsp1407I 1517 TGTACA T^GTACA BspDI 2459 ATCGAT AT^CGAT BspLU11I 4018 ACATGT A^CATGT BsrGI 1517 TGTACA T^GTACA BssHII 398 GCGCGC G^CGCGC CelII 2763 GCTCAGC GC^T[ACGT]AGC ClaI 2459 ATCGAT AT^CGAT DrdI 409 GACGAAGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2158 GACCTACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC KpnI 1510 GGTACC GGTAC^C MamI 639 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 4030 GCCGGC GCC^GGC NgoMIV 4030 GCCGGC G^CCGGC PshAI 3446 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2761 GAGCTC GAGCT^C SapI 3763 GCTCTTC GCTCTTC(1/4) SspBI 1517 TGTACA T^GTACA XmnI 36 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 418 GTCTAC GT^[AC][GT]AC 863 GTCTAC AccIII 914 TCCGGA T^CCGGA 3746 TCCGGA AflIII 1265 ACGTGT A^C[GA][CT]GT 4018 ACATGT ApaI 3842 GGGCCC GGGCC^C 4077 GGGCCC AviII 336 TGCGCA TGC^GCA 782 TGCGCA BamHI 327 GGATCC G^GATCC 849 GGATCC BbvCI 3642 CCTCAGC CCTCAGC(-5/-2) 3852 GCTGAGG BseAI 914 TCCGGA T^CCGGA 3746 TCCGGA BsmI 556 GAATGC GAATGC(1/-1) 2285 GCATTC BspEI 914 TCCGGA T^CCGGA 3746 TCCGGA BsrBI 1596 GAGCGG CCGCTC(-3/-3) 3883 GAGCGG BsrDI 2435 GCAATG GCAATG(2/0) 2488 GCAATG BsrFI 2796 ACCGGC [GA]^CCGG[CT] 4030 GCCGGC BstAPI 339 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1464 GCAGTGGCTGC BstEII 1047 GGTGACC G^GT[ACGT]ACC 3399 GGTCACC BstPI 1047 GGTGACC G^GT[ACGT]ACC 3399 GGTCACC Cfr10I 2796 ACCGGC [GA]^CCGG[CT] 4030 GCCGGC EcoO65I 1047 GGTGACC G^GT[ACGT]ACC 3399 GGTCACC FspI 336 TGCGCA TGC^GCA 782 TGCGCA HindIII 3202 AAGCTT A^AGCTT 3987 AAGCTT KspI 570 CCGCGG CCGC^GG 1235 CCGCGG MroI 914 TCCGGA T^CCGGA 3746 TCCGGA MseI 1226 TTAA T^TAA 1607 TTAA SacII 570 CCGCGG CCGC^GG 1235 CCGCGG SbfI 2022 CCTGCAGG CCTGCA^GG 2055 CCTGCAGG ScaI 549 AGTACT AGT^ACT 2182 AGTACT Sse8387I 2022 CCTGCAGG CCTGCA^GG 2055 CCTGCAGG Tru9I 1226 TTAA T^TAA 1607 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 127 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BfaI BglI BglII BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI