(Linear) (Six-base) MAPSORT of: KIEE6731.seq Check: 4391 from: 1 to: 4158 KIEE6731.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1134 2049 4158 Size: 1134 915 2109 Fragments arranged by size: 2109 1134 915 AccI GT'mk_AC Cuts at: 0 419 864 4158 Size: 419 445 3294 Fragments arranged by size: 3294 445 419 Acc65I G'GTAC_C Cuts at: 0 1510 4158 Size: 1510 2648 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 281 770 1025 1168 1764 4158 Size: 281 489 255 143 596 2394 Fragments arranged by size: 2394 596 489 281 255 143 AflII C'TTAA_G Cuts at: 0 1606 4158 Size: 1606 2552 AflIII A'CryG_T Cuts at: 0 1265 4018 4158 Size: 1265 2753 140 Fragments arranged by size: 2753 1265 140 AhdI GACnn_n'nnGTC Cuts at: 0 2163 4158 Size: 2163 1995 AleI CACnn'nnGTG Cuts at: 0 427 976 1237 4158 Size: 427 549 261 2921 Fragments arranged by size: 2921 549 427 261 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 965 4158 Size: 965 3193 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2366 2398 4158 Size: 2366 32 1760 Fragments arranged by size: 2366 1760 32 AlwNI CAG_nnn'CTG Cuts at: 0 1078 1161 1383 1470 1792 2001 2131 3692 Size: 1078 83 222 87 322 209 130 1561 Cuts at: 3692 4158 Size: 466 Fragments arranged by size: 1561 1078 466 322 222 209 130 87 83 ApaI G_GGCC'C Cuts at: 0 3846 4081 4158 Size: 3846 235 77 Fragments arranged by size: 3846 235 77 ApaLI G'TGCA_C Cuts at: 0 385 4158 Size: 385 3773 AvaI C'yCGr_G Cuts at: 0 227 2614 2716 3998 4073 4158 Size: 227 2387 102 1282 75 85 Fragments arranged by size: 2387 1282 227 102 85 75 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 2340 2373 4158 Size: 2340 33 1785 Fragments arranged by size: 2340 1785 33 BamHI G'GATC_C Cuts at: 0 327 849 4158 Size: 327 522 3309 Fragments arranged by size: 3309 522 327 BanI G'GyrC_C Cuts at: 0 361 392 1510 2426 2505 2741 3338 3392 Size: 361 31 1118 916 79 236 597 54 Cuts at: 3392 3537 4009 4158 Size: 145 472 149 Fragments arranged by size: 1118 916 597 472 361 236 149 145 79 54 31 BanII G_rGCy'C Cuts at: 0 239 1674 1919 2033 2615 2672 2723 2765 Size: 239 1435 245 114 582 57 51 42 Cuts at: 2765 3377 3846 4054 4072 4081 4158 Size: 612 469 208 18 9 77 Fragments arranged by size: 1435 612 582 469 245 239 208 114 77 57 51 42 18 9 BbeI G_GCGC'C Cuts at: 0 365 396 2745 4158 Size: 365 31 2349 1413 Fragments arranged by size: 2349 1413 365 31 BbsI GAAGACnn'nnnn_ Cuts at: 0 1625 4158 Size: 1625 2533 BbvCI CC'TCA_GC Cuts at: 0 3643 3853 4158 Size: 3643 210 305 Fragments arranged by size: 3643 305 210 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2643 2677 4158 Size: 2643 34 1481 Fragments arranged by size: 2643 1481 34 BciVI GTATCCnnnnn_n' Cuts at: 0 3579 4158 Size: 3579 579 BglI GCCn_nnn'nGGC Cuts at: 0 1194 2704 3546 4158 Size: 1194 1510 842 612 Fragments arranged by size: 1510 1194 842 612 BglII A'GATC_T Cuts at: 0 213 2420 3126 4158 Size: 213 2207 706 1032 Fragments arranged by size: 2207 1032 706 213 BlpI GC'TnA_GC Cuts at: 0 2764 4158 Size: 2764 1394 Bme1580I G_kGCm'C Cuts at: 0 389 566 2481 2510 2567 2574 3028 3343 Size: 389 177 1915 29 57 7 454 315 Cuts at: 3343 3542 3681 3846 3926 4012 4081 4158 Size: 199 139 165 80 86 69 77 Fragments arranged by size: 1915 454 389 315 199 177 165 139 86 80 77 69 57 29 7 BmgBI CAC'GTC Cuts at: 0 1032 1760 4158 Size: 1032 728 2398 Fragments arranged by size: 2398 1032 728 BmrI ACTGGGnnnn_n' Cuts at: 0 1035 1166 1433 1632 1649 1754 2077 2297 Size: 1035 131 267 199 17 105 323 220 Cuts at: 2297 2561 2569 3644 4158 Size: 264 8 1075 514 Fragments arranged by size: 1075 1035 514 323 267 264 220 199 131 105 17 8 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 789 2844 3336 3396 3575 4158 Size: 789 2055 492 60 179 583 Fragments arranged by size: 2055 789 583 492 179 60 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 618 784 1100 1336 1345 1471 1852 Size: 60 558 166 316 236 9 126 381 Cuts at: 1852 2189 2629 2641 2852 3140 3159 3331 3366 Size: 337 440 12 211 288 19 172 35 Cuts at: 3366 3901 4038 4158 Size: 535 137 120 Fragments arranged by size: 558 535 440 381 337 316 288 236 211 172 166 137 126 120 60 35 19 12 9 Bpu10I CC'TnA_GC Cuts at: 0 3643 3853 4158 Size: 3643 210 305 Fragments arranged by size: 3643 305 210 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 604 664 4158 Size: 604 60 3494 Fragments arranged by size: 3494 604 60 BsaI GGTCTCn'nnnn_ Cuts at: 0 228 1099 2518 4158 Size: 228 871 1419 1640 Fragments arranged by size: 1640 1419 871 228 BsaAI yAC'GTr Cuts at: 0 1266 4158 Size: 1266 2892 BsaBI GATnn'nnATC Cuts at: 0 643 4158 Size: 643 3515 BsaHI Gr'CG_yC Cuts at: 0 362 393 2742 4158 Size: 362 31 2349 1416 Fragments arranged by size: 2349 1416 362 31 BsaWI w'CCGG_w Cuts at: 0 174 914 3746 4158 Size: 174 740 2832 412 Fragments arranged by size: 2832 740 412 174 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1073 1103 2312 2342 2366 2396 3182 3212 Size: 1073 30 1209 30 24 30 786 30 Cuts at: 3212 3411 3441 4080 4086 4110 4116 4158 Size: 199 30 639 6 24 6 42 Fragments arranged by size: 1209 1073 786 639 199 42 30 30 30 30 30 24 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 93 1386 2308 2506 3094 3143 3254 3316 Size: 93 1293 922 198 588 49 111 62 Cuts at: 3316 3319 3346 4158 Size: 3 27 812 Fragments arranged by size: 1293 922 812 588 198 111 93 62 49 27 3 BseYI C'CCAG_C Cuts at: 0 355 1052 1255 1785 1918 2253 2390 2474 Size: 355 697 203 530 133 335 137 84 Cuts at: 2474 3069 3376 3491 3572 3582 3839 3935 4158 Size: 595 307 115 81 10 257 96 223 Fragments arranged by size: 697 595 530 355 335 307 257 223 203 137 133 115 96 84 81 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1261 1552 1846 2605 3241 4158 Size: 1261 291 294 759 636 917 Fragments arranged by size: 1261 917 759 636 294 291 BsiEI CG_ry'CG Cuts at: 0 406 3032 4031 4158 Size: 406 2626 999 127 Fragments arranged by size: 2626 999 406 127 BsiHKAI G_wGCw'C Cuts at: 0 389 2765 3741 3947 4158 Size: 389 2376 976 206 211 Fragments arranged by size: 2376 976 389 211 206 BsmI GAATG_Cn' Cuts at: 0 562 2285 4158 Size: 562 1723 1873 Fragments arranged by size: 1873 1723 562 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1932 4158 Size: 1932 2226 Bsp1286I G_dGCh'C Cuts at: 0 239 389 566 1674 1919 2033 2481 2510 Size: 239 150 177 1108 245 114 448 29 Cuts at: 2510 2567 2574 2615 2672 2723 2765 3028 3343 Size: 57 7 41 57 51 42 263 315 Cuts at: 3343 3377 3542 3681 3741 3846 3926 3947 4012 Size: 34 165 139 60 105 80 21 65 Cuts at: 4012 4054 4072 4081 4158 Size: 42 18 9 77 Fragments arranged by size: 1108 448 315 263 245 239 177 165 150 139 114 105 80 77 65 60 57 57 51 42 42 41 34 29 21 18 9 7 BspEI T'CCGG_A Cuts at: 0 914 3746 4158 Size: 914 2832 412 Fragments arranged by size: 2832 914 412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 20 893 1134 2049 4158 Size: 20 873 241 915 2109 Fragments arranged by size: 2109 915 873 241 20 BsrBI CCG'CTC Cuts at: 0 1598 3885 4158 Size: 1598 2287 273 Fragments arranged by size: 2287 1598 273 BsrDI GCAATG_nn' Cuts at: 0 2442 2495 4158 Size: 2442 53 1663 Fragments arranged by size: 2442 1663 53 BsrFI r'CCGG_y Cuts at: 0 2796 4030 4158 Size: 2796 1234 128 Fragments arranged by size: 2796 1234 128 BsrGI T'GTAC_A Cuts at: 0 1517 4158 Size: 1517 2641 BssHII G'CGCG_C Cuts at: 0 398 4158 Size: 398 3760 BstAPI GCAn_nnn'nTGC Cuts at: 0 345 1470 4158 Size: 345 1125 2688 Fragments arranged by size: 2688 1125 345 BstEII G'GTnAC_C Cuts at: 0 1047 3399 4158 Size: 1047 2352 759 Fragments arranged by size: 2352 1047 759 BstXI CCAn_nnnn'nTGG Cuts at: 0 2040 2398 3335 4158 Size: 2040 358 937 823 Fragments arranged by size: 2040 937 823 358 BstYI r'GATC_y Cuts at: 0 213 327 849 911 2420 3126 4158 Size: 213 114 522 62 1509 706 1032 Fragments arranged by size: 1509 1032 706 522 213 114 62 Bsu36I CC'TnA_GG Cuts at: 0 2132 2291 3120 4158 Size: 2132 159 829 1038 Fragments arranged by size: 2132 1038 829 159 BtgI C'CryG_G Cuts at: 0 570 648 1235 1981 2852 3096 4025 4158 Size: 570 78 587 746 871 244 929 133 Fragments arranged by size: 929 871 746 587 570 244 133 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 701 1506 4158 Size: 701 805 2652 Fragments arranged by size: 2652 805 701 BtsI GCAGTG_nn' Cuts at: 0 539 1471 1898 2807 3681 3900 4158 Size: 539 932 427 909 874 219 258 Fragments arranged by size: 932 909 874 539 427 258 219 ClaI AT'CG_AT Cuts at: 0 2460 4158 Size: 2460 1698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 913 948 1025 1060 3794 3829 4158 Size: 913 35 77 35 2734 35 329 Fragments arranged by size: 2734 913 329 77 35 35 35 DrdI GACnn_nn'nnGTC Cuts at: 0 415 4158 Size: 415 3743 EaeI y'GGCC_r Cuts at: 0 56 311 403 573 1301 1502 1565 2540 Size: 56 255 92 170 728 201 63 975 Cuts at: 2540 3017 3029 3241 4028 4083 4158 Size: 477 12 212 787 55 75 Fragments arranged by size: 975 787 728 477 255 212 201 170 92 75 63 56 55 12 EagI C'GGCC_G Cuts at: 0 403 3029 4028 4158 Size: 403 2626 999 130 Fragments arranged by size: 2626 999 403 130 EarI CTCTTCn'nnn_ Cuts at: 0 373 620 3770 3824 3953 4047 4158 Size: 373 247 3150 54 129 94 111 Fragments arranged by size: 3150 373 247 129 111 94 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 997 2020 4158 Size: 997 1023 2138 Fragments arranged by size: 2138 1023 997 EcoICRI GAG'CTC Cuts at: 0 2763 4158 Size: 2763 1395 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 281 618 770 784 1025 1100 1168 Size: 60 221 337 152 14 241 75 68 Cuts at: 1168 1336 1345 1471 1764 1852 2189 2629 2641 Size: 168 9 126 293 88 337 440 12 Cuts at: 2641 2852 3140 3159 3331 3366 3901 4038 4158 Size: 211 288 19 172 35 535 137 120 Fragments arranged by size: 535 440 337 337 293 288 241 221 211 172 168 152 137 126 120 88 75 68 60 35 19 14 12 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 835 1303 2135 3774 4158 Size: 835 468 832 1639 384 Fragments arranged by size: 1639 835 832 468 384 EcoO109I rG'GnC_Cy Cuts at: 0 1158 1308 1905 1977 2044 2128 2210 2298 Size: 1158 150 597 72 67 84 82 88 Cuts at: 2298 2823 3046 3133 3730 3843 4077 4078 4158 Size: 525 223 87 597 113 234 1 80 Fragments arranged by size: 1158 597 597 525 234 223 150 113 88 87 84 82 80 72 67 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3220 4158 Size: 3220 938 FspI TGC'GCA Cuts at: 0 338 784 4158 Size: 338 446 3374 Fragments arranged by size: 3374 446 338 FspAI rTGC'GCAy Cuts at: 0 338 4158 Size: 338 3820 HaeII r_GCGC'y Cuts at: 0 118 365 396 1200 2710 2745 4158 Size: 118 247 31 804 1510 35 1413 Fragments arranged by size: 1510 1413 804 247 118 35 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1059 1091 2149 2181 3216 3248 4080 4086 Size: 1059 32 1058 32 1035 32 832 6 Cuts at: 4086 4112 4118 4158 Size: 26 6 40 Fragments arranged by size: 1059 1058 1035 832 40 32 32 32 26 6 6 HindIII A'AGCT_T Cuts at: 0 3202 3987 4158 Size: 3202 785 171 Fragments arranged by size: 3202 785 171 KasI G'GCGC_C Cuts at: 0 361 392 2741 4158 Size: 361 31 2349 1417 Fragments arranged by size: 2349 1417 361 31 KpnI G_GTAC'C Cuts at: 0 1514 4158 Size: 1514 2644 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 95 740 2135 4078 4158 Size: 95 645 1395 1943 80 Fragments arranged by size: 1943 1395 645 95 80 MscI TGG'CCA Cuts at: 0 313 1303 1504 1567 3243 4158 Size: 313 990 201 63 1676 915 Fragments arranged by size: 1676 990 915 313 201 63 MslI CAynn'nnrTG Cuts at: 0 427 700 947 976 1237 3953 4158 Size: 427 273 247 29 261 2716 205 Fragments arranged by size: 2716 427 273 261 247 205 29 MspA1I CmG'CkG Cuts at: 0 359 572 748 1237 1559 1792 1830 1850 Size: 359 213 176 489 322 233 38 20 Cuts at: 1850 2091 2589 2690 3073 3939 4158 Size: 241 498 101 383 866 219 Fragments arranged by size: 866 498 489 383 359 322 241 233 219 213 176 101 38 20 NaeI GCC'GGC Cuts at: 0 4032 4158 Size: 4032 126 NarI GG'CG_CC Cuts at: 0 362 393 2742 4158 Size: 362 31 2349 1416 Fragments arranged by size: 2349 1416 362 31 NcoI C'CATG_G Cuts at: 0 1981 2852 3096 4158 Size: 1981 871 244 1062 Fragments arranged by size: 1981 1062 871 244 NgoMIV G'CCGG_C Cuts at: 0 4030 4158 Size: 4030 128 NspI r_CATG'y Cuts at: 0 699 946 3510 3873 4022 4158 Size: 699 247 2564 363 149 136 Fragments arranged by size: 2564 699 363 247 149 136 PasI CC'CwG_GG Cuts at: 0 2933 4158 Size: 2933 1225 PciI A'CATG_T Cuts at: 0 4018 4158 Size: 4018 140 PflMI CCAn_nnn'nTGG Cuts at: 0 1136 1744 1792 4158 Size: 1136 608 48 2366 Fragments arranged by size: 2366 1136 608 48 PfoI T'CCnGG_A Cuts at: 0 513 595 1077 2065 4158 Size: 513 82 482 988 2093 Fragments arranged by size: 2093 988 513 482 82 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2366 2398 4158 Size: 2366 32 1760 Fragments arranged by size: 2366 1760 32 PpuMI rG'GwC_Cy Cuts at: 0 1158 1977 2044 2210 3046 3133 3730 4158 Size: 1158 819 67 166 836 87 597 428 Fragments arranged by size: 1158 836 819 597 428 166 87 67 PshAI GACnn'nnGTC Cuts at: 0 3450 4158 Size: 3450 708 PspOMI G'GGCC_C Cuts at: 0 3842 4077 4158 Size: 3842 235 81 Fragments arranged by size: 3842 235 81 PstI C_TGCA'G Cuts at: 0 32 1417 1475 1755 1859 2027 2060 2469 Size: 32 1385 58 280 104 168 33 409 Cuts at: 2469 3089 3630 4158 Size: 620 541 528 Fragments arranged by size: 1385 620 541 528 409 280 168 104 58 33 32 PvuII CAG'CTG Cuts at: 0 748 1559 1792 1830 2091 2589 4158 Size: 748 811 233 38 261 498 1569 Fragments arranged by size: 1569 811 748 498 261 233 38 SacI G_AGCT'C Cuts at: 0 2765 4158 Size: 2765 1393 SacII CC_GC'GG Cuts at: 0 573 1238 4158 Size: 573 665 2920 Fragments arranged by size: 2920 665 573 SanDI GG'GwC_CC Cuts at: 0 2044 2210 3133 4158 Size: 2044 166 923 1025 Fragments arranged by size: 2044 1025 923 166 SapI GCTCTTCn'nnn_ Cuts at: 0 3770 4158 Size: 3770 388 SbfI CC_TGCA'GG Cuts at: 0 2027 2060 4158 Size: 2027 33 2098 Fragments arranged by size: 2098 2027 33 ScaI AGT'ACT Cuts at: 0 551 2184 4158 Size: 551 1633 1974 Fragments arranged by size: 1974 1633 551 SfcI C'TryA_G Cuts at: 0 28 1413 1471 1554 1646 1751 1855 2023 Size: 28 1385 58 83 92 105 104 168 Cuts at: 2023 2056 2161 2465 3054 3085 3626 3771 4158 Size: 33 105 304 589 31 541 145 387 Fragments arranged by size: 1385 589 541 387 304 168 145 105 105 104 92 83 58 33 31 28 SfoI GGC'GCC Cuts at: 0 363 394 2743 4158 Size: 363 31 2349 1415 Fragments arranged by size: 2349 1415 363 31 SmaI CCC'GGG Cuts at: 0 2616 4000 4075 4158 Size: 2616 1384 75 83 Fragments arranged by size: 2616 1384 83 75 SmlI C'TyrA_G Cuts at: 0 619 679 1606 4158 Size: 619 60 927 2552 Fragments arranged by size: 2552 927 619 60 SrfI GCCC'GGGC Cuts at: 0 2616 4158 Size: 2616 1542 StuI AGG'CCT Cuts at: 0 1777 3603 3964 4158 Size: 1777 1826 361 194 Fragments arranged by size: 1826 1777 361 194 StyI C'CwwG_G Cuts at: 0 1304 1981 2238 2333 2852 2859 3096 3185 Size: 1304 677 257 95 519 7 237 89 Cuts at: 3185 3543 3758 4158 Size: 358 215 400 Fragments arranged by size: 1304 677 519 400 358 257 237 215 95 89 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2865 2918 3604 3965 4158 Size: 2865 53 686 361 193 Fragments arranged by size: 2865 686 361 193 53 TatI w'GTAC_w Cuts at: 0 549 1517 2182 4158 Size: 549 968 665 1976 Fragments arranged by size: 1976 968 665 549 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 509 541 594 626 1073 1105 1450 1482 Size: 509 32 53 32 447 32 345 32 Cuts at: 1482 2312 2344 3182 3214 4158 Size: 830 32 838 32 944 Fragments arranged by size: 944 838 830 509 447 345 53 32 32 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1311 1380 2553 2850 3175 3689 4158 Size: 1311 69 1173 297 325 514 469 Fragments arranged by size: 1311 1173 514 469 325 297 69 XmaI C'CCGG_G Cuts at: 0 2614 3998 4073 4158 Size: 2614 1384 75 85 Fragments arranged by size: 2614 1384 85 75 XmnI GAAnn'nnTTC Cuts at: 0 40 4158 Size: 40 4118 Enzymes that do cut: AarI AccI Acc65I AcuI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII SanDI SapI SbfI ScaI SfcI SfoI SmaI SmlI SrfI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AgeI ApoI AscI AseI AsiSI AvrII BclI BfrBI BmtI BsiWI BspHI BssSI BstBI BstZ17I DraI DraIII EcoRI EcoRV FseI HincII HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SalI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI ZraI