################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BclI BfrI BglII BlnI BsaAI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrDI BsrFI Bst1107I BstBI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MflI MluI MroI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI StyI SwaI TfiI Tth111I XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AatII 2155 GACGTC GACGT^C AccI 1156 GTAGAC GT^[AC][GT]AC BbsI 43 GTCTTC GAAGAC(2/6) BbvCI 1474 GCTGAGG CCTCAGC(-5/-2) BcgI 1224 CGACCTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1183 GGATAC GTATCC(6/5) BglI 2086 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 43 GTCTTC GAAGAC(2/6) BsaBI 699 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 1032 CGACCG CG[GA][CT]^CG Bsp1407I 626 TGTACA T^GTACA BsrGI 626 TGTACA T^GTACA BssHII 1330 GCGCGC G^CGCGC BssSI 1150 CACGAG CACGAG(-5/-1) BstAPI 1246 GCACATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1086 GGTCACC G^GT[ACGT]ACC BstPI 1086 GGTCACC G^GT[ACGT]ACC EcoO65I 1086 GGTCACC G^GT[ACGT]ACC EcoT22I 469 ATGCAT ATGCA^T HgaI 175 GACGC GACGC(5/10) MamI 699 GATTATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 638 CAATTG C^AATTG MunI 638 CAATTG C^AATTG NsiI 469 ATGCAT ATGCA^T SmaI 949 CCCGGG CCC^GGG SspBI 626 TGTACA T^GTACA XbaI 834 TCTAGA T^CTAGA XmaCI 949 CCCGGG C^CCGGG XmaI 949 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 479 AAATTT [GA]^AATT[CT] 1041 GAATTT ApaI 1016 GGGCCC GGGCC^C 1286 GGGCCC ApoI 479 AAATTT [GA]^AATT[CT] 1041 GAATTT AspHI 1859 GTGCTC G[AT]GC[AT]^C 2007 GAGCAC AvaI 949 CCCGGG C^[CT]CG[GA]G 1050 CCCGAG BanI 74 GGCGCC G^G[CT][GA]CC 2143 GGCGCC BanII 1016 GGGCCC G[GA]GC[CT]^C 1286 GGGCCC BbeI 74 GGCGCC GGCGC^C 2143 GGCGCC BlpI 561 GCTCAGC GC^T[ACGT]AGC 1564 GCTCAGC Bpu1102I 561 GCTCAGC GC^T[ACGT]AGC 1564 GCTCAGC BsaI 376 GAGACC GGTCTC(1/5) 1440 GGTCTC BseRI 1912 GAGGAG GAGGAG(10/8) 2004 GAGGAG BsiHKAI 1859 GTGCTC G[AT]GC[AT]^C 2007 GAGCAC BsoBI 949 CCCGGG C^[CT]CG[GA]G 1050 CCCGAG BstXI 500 CCAAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1262 CCAACCGAGTGG CelII 561 GCTCAGC GC^T[ACGT]AGC 1564 GCTCAGC DraIII 244 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG 1247 CACATGGTG DsaI 1347 CCACGG C^C[GA][CT]GG 1905 CCACGG EcoNI 315 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1464 CCTGCATGAGG KasI 74 GGCGCC G^GCGCC 2143 GGCGCC MseI 410 TTAA T^TAA 959 TTAA NarI 74 GGCGCC GG^CGCC 2143 GGCGCC PpuMI 609 AGGACCT [GA]G^G[AT]CC[CT] 809 AGGACCC PvuII 1700 CAGCTG CAG^CTG 2149 CAGCTG SfoI 74 GGCGCC GGC^GCC 2143 GGCGCC SphI 557 GCATGC GCATG^C 722 GCATGC Tru9I 410 TTAA T^TAA 959 TTAA XcmI 305 CCAACGGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1170 CCAGACGGCCTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AvaII BalI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I