(Circular) (Six-base) MAPSORT of: pFN21AE3293.seq Check: 9428 from: 1 to: 7000 pFN21AE3293 7000 bp With 182 enzymes: SgfI * July 6, 2012 17:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3124 3648 3124 Size: 524 6476 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4164 4810 278 Size: 53 83 186 936 2628 646 2468 Fragments arranged by size: 2628 2468 936 646 186 83 53 AccI GT'mk_AC Cuts at: 1937 3162 4343 1937 Size: 1225 1181 4594 Fragments arranged by size: 4594 1225 1181 Acc65I G'GTAC_C Cuts at: 4321 4321 Size: 7000 AclI AA'CG_TT Cuts at: 1563 5127 5500 1563 Size: 3564 373 3063 Fragments arranged by size: 3564 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2086 3011 3035 3665 5063 6447 6715 1632 Size: 454 925 24 630 1398 1384 268 1917 Fragments arranged by size: 1917 1398 1384 925 630 454 268 24 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6803 AflIII A'CryG_T Cuts at: 1203 5899 6876 1203 Size: 4696 977 1327 Fragments arranged by size: 4696 1327 977 AgeI A'CCGG_T Cuts at: 5831 5831 Size: 7000 AhdI GACnn_n'nnGTC Cuts at: 5729 5729 Size: 7000 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7000 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3755 3918 4205 1184 Size: 2571 163 287 3979 Fragments arranged by size: 3979 2571 287 163 AlwNI CAG_nnn'CTG Cuts at: 2133 2284 2349 2986 4743 6315 2133 Size: 151 65 637 1757 1572 2818 Fragments arranged by size: 2818 1757 1572 637 151 65 ApaI G_GGCC'C Cuts at: 3025 3295 3025 Size: 270 6730 ApaLI G'TGCA_C Cuts at: 5057 6213 5057 Size: 1156 5844 ApoI r'AATT_y Cuts at: 1493 2484 3046 4309 4535 4687 6796 1493 Size: 991 562 1263 226 152 2109 1697 Fragments arranged by size: 2109 1697 1263 991 562 226 152 AseI AT'TA_AT Cuts at: 160 1032 5554 160 Size: 872 4522 1606 Fragments arranged by size: 4522 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 AvaI C'yCGr_G Cuts at: 1870 1943 2954 3055 4325 1870 Size: 73 1011 101 1270 4545 Fragments arranged by size: 4545 1270 1011 101 73 BamHI G'GATC_C Cuts at: 4330 4697 4330 Size: 367 6633 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2079 4148 4321 5776 618 Size: 325 217 627 292 2069 173 1455 1842 Fragments arranged by size: 2069 1842 1455 627 325 292 217 173 BanII G_rGCy'C Cuts at: 1391 3025 3295 4319 1391 Size: 1634 270 1024 4072 Fragments arranged by size: 4072 1634 1024 270 BbeI G_GCGC'C Cuts at: 2083 4152 2083 Size: 2069 4931 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2041 928 Size: 971 142 5887 Fragments arranged by size: 5887 971 142 BbvCI CC'TCA_GC Cuts at: 3480 3480 Size: 7000 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3218 3252 5191 5225 1903 Size: 34 1281 34 1939 34 3678 Fragments arranged by size: 3678 1939 1281 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3182 4892 6102 3182 Size: 1710 1210 4080 Fragments arranged by size: 4080 1710 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7000 BfrBI ATG'CAT Cuts at: 2476 2476 Size: 7000 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4097 5611 136 Size: 107 122 71 631 605 2425 1514 1525 Fragments arranged by size: 2425 1525 1514 631 605 122 107 71 BglII A'GATC_T Cuts at: 6995 6995 Size: 7000 BlpI GC'TnA_GC Cuts at: 2567 3570 4417 2567 Size: 1003 847 5150 Fragments arranged by size: 5150 1003 847 Bme1580I G_kGCm'C Cuts at: 1790 3025 3241 3295 5061 6217 1790 Size: 1235 216 54 1766 1156 2573 Fragments arranged by size: 2573 1766 1235 1156 216 54 BmgBI CAC'GTC Cuts at: 1318 3166 1318 Size: 1848 5152 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4731 5689 442 Size: 416 53 83 399 24 3314 958 1753 Fragments arranged by size: 3314 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7000 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2568 2568 Size: 7000 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2342 2615 3842 5660 1239 Size: 1103 273 1227 1818 2579 Fragments arranged by size: 2579 1818 1227 1103 273 Bpu10I CC'TnA_GC Cuts at: 2360 3480 3743 2360 Size: 1120 263 5617 Fragments arranged by size: 5617 1120 263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2426 3782 4091 5125 5990 6288 2426 Size: 1356 309 1034 865 298 3138 Fragments arranged by size: 3138 1356 1034 865 309 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2375 3451 5663 882 Size: 617 876 1076 2212 2219 Fragments arranged by size: 2219 2212 1076 876 617 BsaAI yAC'GTr Cuts at: 493 1204 4791 493 Size: 711 3587 2702 Fragments arranged by size: 3587 2702 711 BsaBI GATnn'nnATC Cuts at: 2708 6994 2708 Size: 4286 2714 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2080 2181 4149 4161 Size: 53 83 186 936 547 101 1968 12 Cuts at: 4161 4807 5189 5824 275 Size: 646 382 635 1451 Fragments arranged by size: 1968 1451 936 646 635 547 382 186 101 83 53 12 BsaWI w'CCGG_w Cuts at: 5433 5831 6105 6252 6663 5433 Size: 398 274 147 411 5770 Fragments arranged by size: 5770 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4185 4215 1178 Size: 30 165 30 2782 30 3963 Fragments arranged by size: 3963 2782 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3932 4024 1186 Size: 2746 92 4162 Fragments arranged by size: 4162 2746 92 BseYI C'CCAG_C Cuts at: 2032 2775 2805 3288 4152 6203 6699 2032 Size: 743 30 483 864 2051 496 2333 Fragments arranged by size: 2333 2051 864 743 496 483 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2390 3284 3393 3581 3680 3878 3888 Size: 573 654 894 109 188 99 198 10 Cuts at: 3888 4165 1163 Size: 277 3998 Fragments arranged by size: 3998 894 654 573 277 198 188 109 99 10 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3040 4450 5211 6239 1540 Size: 275 186 1039 1410 761 1028 2301 Fragments arranged by size: 2301 1410 1039 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3868 4016 5061 5146 6217 3868 Size: 148 1045 85 1071 4651 Fragments arranged by size: 4651 1071 1045 148 85 BsmI GAATG_Cn' Cuts at: 4511 4604 4511 Size: 93 6907 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3025 3241 3295 3868 4016 4319 5061 Size: 399 1235 216 54 573 148 303 742 Cuts at: 5061 5146 6217 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1235 1071 742 573 399 303 216 148 85 54 BspEI T'CCGG_A Cuts at: 6663 6663 Size: 7000 BspHI T'CATG_A Cuts at: 4889 6619 4889 Size: 1730 5270 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3124 3477 3648 4355 844 Size: 2280 353 171 707 3489 Fragments arranged by size: 3489 2280 707 353 171 BsrBI CCG'CTC Cuts at: 1394 1823 3613 3656 3681 4887 1394 Size: 429 1790 43 25 1206 3507 Fragments arranged by size: 3507 1790 1206 429 43 25 BsrDI GCAATG_nn' Cuts at: 65 1248 5495 5669 65 Size: 1183 4247 174 1396 Fragments arranged by size: 4247 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5644 5831 1700 Size: 110 3834 187 2869 Fragments arranged by size: 3834 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2631 96 Size: 2535 4465 BssHII G'CGCG_C Cuts at: 1925 3335 1925 Size: 1410 5590 BssSI C'ACGA_G Cuts at: 3155 5060 6072 3155 Size: 1905 1012 4083 Fragments arranged by size: 4083 1905 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3257 794 Size: 2463 4537 BstBI TT'CG_AA Cuts at: 5804 5804 Size: 7000 BstEII G'GTnAC_C Cuts at: 1184 3091 1184 Size: 1907 5093 BstXI CCAn_nnnn'nTGG Cuts at: 2512 3274 2512 Size: 762 6238 BstYI r'GATC_y Cuts at: 1972 4330 4697 5082 5099 6551 6637 6649 6995 Size: 2358 367 385 17 1452 86 12 346 Cuts at: 6995 1972 Size: 1977 Fragments arranged by size: 2358 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3352 3910 513 Size: 552 939 1348 558 3603 Fragments arranged by size: 3603 1348 939 558 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6261 Fragments arranged by size: 6261 683 56 BtsI GCAGTG_nn' Cuts at: 2873 3130 3296 3330 3593 3692 3699 3935 3950 Size: 257 166 34 263 99 7 236 15 Cuts at: 3950 4516 5310 5330 2873 Size: 566 794 20 4543 Fragments arranged by size: 4543 794 566 263 257 236 166 99 34 20 15 7 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7000 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6965 DraI TTT'AAA Cuts at: 4304 4304 Size: 7000 DraIII CAC_nnn'GTG Cuts at: 2254 3257 2254 Size: 1003 5997 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6007 809 Size: 507 4691 1802 Fragments arranged by size: 4691 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2307 2391 Size: 54 1425 50 275 18 258 219 84 Cuts at: 2391 2762 2916 3504 3517 4098 4257 4447 5336 Size: 371 154 588 13 581 159 190 889 Cuts at: 5336 6965 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 588 581 371 275 258 219 190 159 154 84 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 4447 1537 Size: 275 2635 4090 Fragments arranged by size: 4090 2635 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2819 3402 3641 4930 1353 Size: 1466 583 239 1289 3423 Fragments arranged by size: 3423 1466 1289 583 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2152 5587 5961 6107 387 Size: 1064 138 563 3435 374 146 1280 Fragments arranged by size: 3435 1280 1064 563 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2086 2342 2615 3011 3035 3665 3842 Size: 393 454 256 273 396 24 630 177 Cuts at: 3842 5063 5660 6447 6715 1239 Size: 1221 597 787 268 1524 Fragments arranged by size: 1524 1221 787 630 597 454 396 393 273 268 256 177 24 EcoNI CCTnn'n_nnAGG Cuts at: 2324 3473 2324 Size: 1149 5851 EcoO109I rG'GnC_Cy Cuts at: 2608 2615 2815 2911 3022 3292 3357 2608 Size: 7 200 96 111 270 65 6251 Fragments arranged by size: 6251 270 200 111 96 65 7 EcoRI G'AATT_C Cuts at: 4309 4687 4309 Size: 378 6622 FspI TGC'GCA Cuts at: 5506 5506 Size: 7000 HaeII r_GCGC'y Cuts at: 1678 2083 2134 4152 6147 1678 Size: 405 51 2018 1995 2531 Fragments arranged by size: 2531 2018 1995 405 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2984 3016 3817 3849 5641 Size: 32 135 32 1412 32 801 32 1792 Cuts at: 5641 5673 5715 5747 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1792 1412 801 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4344 4590 669 Size: 988 281 2406 246 3079 Fragments arranged by size: 3079 2406 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7000 HpaI GTT'AAC Cuts at: 4590 4590 Size: 7000 KasI G'GCGC_C Cuts at: 2079 4148 2079 Size: 2069 4931 KpnI G_GTAC'C Cuts at: 4325 4325 Size: 7000 MfeI C'AATT_G Cuts at: 2643 4599 2643 Size: 1956 5044 MluI A'CGCG_T Cuts at: 6876 6876 Size: 7000 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3060 3543 3658 6114 6298 1305 Size: 1755 483 115 2456 184 2007 Fragments arranged by size: 2456 2007 1755 483 184 115 MscI TGG'CCA Cuts at: 10 64 1489 1832 2090 2309 2393 2764 2918 Size: 54 1425 343 258 219 84 371 154 Cuts at: 2918 3519 4259 10 Size: 601 740 2751 Fragments arranged by size: 2751 1425 740 601 371 343 258 219 154 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 2699 4053 4958 5317 5476 518 Size: 648 1362 171 1354 905 359 159 2042 Fragments arranged by size: 2042 1362 1354 905 648 359 171 159 MspA1I CmG'CkG Cuts at: 1606 2094 3707 4156 4416 5093 6241 6486 1606 Size: 488 1613 449 260 677 1148 245 2120 Fragments arranged by size: 2120 1613 1148 677 488 449 260 245 NarI GG'CG_CC Cuts at: 2080 4149 2080 Size: 2069 4931 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5509 Fragments arranged by size: 5509 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7000 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7000 NotI GC'GGCC_GC Cuts at: 4447 4447 Size: 7000 NsiI A_TGCA'T Cuts at: 2478 2478 Size: 7000 NspI r_CATG'y Cuts at: 1132 1229 2476 2480 2566 2731 4358 5903 1132 Size: 97 1247 4 86 165 1627 1545 2229 Fragments arranged by size: 2229 1627 1545 1247 165 97 86 4 PasI CC'CwG_GG Cuts at: 1348 2284 3118 1348 Size: 936 834 5230 Fragments arranged by size: 5230 936 834 PciI A'CATG_T Cuts at: 5899 5899 Size: 7000 PflMI CCAn_nnn'nTGG Cuts at: 1108 2782 3109 3838 1108 Size: 1674 327 729 4270 Fragments arranged by size: 4270 1674 729 327 PfoI T'CCnGG_A Cuts at: 2460 2592 3345 4164 2460 Size: 132 753 819 5296 Fragments arranged by size: 5296 819 753 132 PmeI GTTT'AAAC Cuts at: 4304 4304 Size: 7000 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5069 5101 6582 6614 5069 Size: 32 1481 32 5455 Fragments arranged by size: 5455 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2615 2815 2615 Size: 200 6800 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7000 PsiI TTA'TAA Cuts at: 4570 4570 Size: 7000 PspOMI G'GGCC_C Cuts at: 3021 3291 3021 Size: 270 6730 PstI C_TGCA'G Cuts at: 830 2883 3706 3945 3970 4352 830 Size: 2053 823 239 25 382 3478 Fragments arranged by size: 3478 2053 823 382 239 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7000 PvuII CAG'CTG Cuts at: 3707 4156 3707 Size: 449 6551 SalI G'TCGA_C Cuts at: 1936 4342 1936 Size: 2406 4594 SbfI CC_TGCA'GG Cuts at: 4352 4352 Size: 7000 ScaI AGT'ACT Cuts at: 5248 6960 5248 Size: 1712 5288 SfcI C'TryA_G Cuts at: 826 1045 2741 2879 3702 3941 3966 4348 5483 Size: 219 1696 138 823 239 25 382 1135 Cuts at: 5483 6164 6355 826 Size: 681 191 1471 Fragments arranged by size: 1696 1471 1135 823 681 382 239 219 191 138 25 SfoI GGC'GCC Cuts at: 2081 4150 2081 Size: 2069 4931 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7000 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7000 SmaI CCC'GGG Cuts at: 1872 2956 4327 1872 Size: 1084 1371 4545 Fragments arranged by size: 4545 1371 1084 SmlI C'TyrA_G Cuts at: 820 1017 1943 2441 3761 4106 5104 6005 6267 Size: 197 926 498 1320 345 998 901 262 Cuts at: 6267 820 Size: 1553 Fragments arranged by size: 1553 1320 998 926 901 498 345 262 197 SnaBI TAC'GTA Cuts at: 493 4791 493 Size: 4298 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7000 SphI G_CATG'C Cuts at: 1132 2566 2731 4358 1132 Size: 1434 165 1627 3774 Fragments arranged by size: 3774 1627 1434 165 SspI AAT'ATT Cuts at: 5 52 4924 5 Size: 47 4872 2081 Fragments arranged by size: 4872 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7000 StyI C'CwwG_G Cuts at: 513 1065 2004 4439 513 Size: 552 939 2435 3074 Fragments arranged by size: 3074 2435 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 3788 3851 4256 5027 5044 5197 Size: 1255 774 1434 63 405 771 17 153 Cuts at: 5197 5382 5827 325 Size: 185 445 1498 Fragments arranged by size: 1498 1434 1255 774 771 445 405 185 153 63 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2535 2631 5246 Size: 274 80 33 51 1443 558 96 2615 Cuts at: 5246 6958 96 Size: 1712 138 Fragments arranged by size: 2615 1712 1443 558 274 138 96 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6960 6992 6960 Size: 32 6968 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7000 XbaI T'CTAG_A Cuts at: 2839 4336 2839 Size: 1497 5503 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2317 3182 2317 Size: 865 6135 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7000 XmaI C'CCGG_G Cuts at: 1870 2954 4325 1870 Size: 1084 1371 4545 Fragments arranged by size: 4545 1371 1084 XmnI GAAnn'nnTTC Cuts at: 5129 5129 Size: 7000 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4162 4808 276 Size: 53 83 186 936 2628 646 2468 Fragments arranged by size: 2628 2468 936 646 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI