(Linear) (Six-base) MAPSORT of: KIEE3293.seq Check: 1338 from: 1 to: 2295 KIEE3293.orf With 182 enzymes: SgfI * July 3, 2012 17:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1119 1643 2295 Size: 1119 524 652 Fragments arranged by size: 1119 652 524 AatII G_ACGT'C Cuts at: 0 2159 2295 Size: 2159 136 AccI GT'mk_AC Cuts at: 0 1157 2295 Size: 1157 1138 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 81 1006 1030 1660 2295 Size: 81 925 24 630 635 Fragments arranged by size: 925 635 630 81 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1750 1913 2200 2295 Size: 1750 163 287 95 Fragments arranged by size: 1750 287 163 95 AlwNI CAG_nnn'CTG Cuts at: 0 128 279 344 981 2295 Size: 128 151 65 637 1314 Fragments arranged by size: 1314 637 151 128 65 ApaI G_GGCC'C Cuts at: 0 1020 1290 2295 Size: 1020 270 1005 Fragments arranged by size: 1020 1005 270 ApoI r'AATT_y Cuts at: 0 479 1041 2295 Size: 479 562 1254 Fragments arranged by size: 1254 562 479 AvaI C'yCGr_G Cuts at: 0 949 1050 2295 Size: 949 101 1245 Fragments arranged by size: 1245 949 101 BanI G'GyrC_C Cuts at: 0 74 2143 2295 Size: 74 2069 152 Fragments arranged by size: 2069 152 74 BanII G_rGCy'C Cuts at: 0 1020 1290 2295 Size: 1020 270 1005 Fragments arranged by size: 1020 1005 270 BbeI G_GCGC'C Cuts at: 0 78 2147 2295 Size: 78 2069 148 Fragments arranged by size: 2069 148 78 BbsI GAAGACnn'nnnn_ Cuts at: 0 36 2295 Size: 36 2259 BbvCI CC'TCA_GC Cuts at: 0 1475 2295 Size: 1475 820 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1213 1247 2295 Size: 1213 34 1048 Fragments arranged by size: 1213 1048 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1177 2295 Size: 1177 1118 BfrBI ATG'CAT Cuts at: 0 471 2295 Size: 471 1824 BglI GCCn_nnn'nGGC Cuts at: 0 2092 2295 Size: 2092 203 BlpI GC'TnA_GC Cuts at: 0 562 1565 2295 Size: 562 1003 730 Fragments arranged by size: 1003 730 562 Bme1580I G_kGCm'C Cuts at: 0 1020 1236 1290 2295 Size: 1020 216 54 1005 Fragments arranged by size: 1020 1005 216 54 BmgBI CAC'GTC Cuts at: 0 1161 2295 Size: 1161 1134 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 563 2295 Size: 563 1732 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 610 1837 2295 Size: 337 273 1227 458 Fragments arranged by size: 1227 458 337 273 Bpu10I CC'TnA_GC Cuts at: 0 355 1475 1738 2295 Size: 355 1120 263 557 Fragments arranged by size: 1120 557 355 263 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 421 1777 2086 2295 Size: 421 1356 309 209 Fragments arranged by size: 1356 421 309 209 BsaI GGTCTCn'nnnn_ Cuts at: 0 370 1446 2295 Size: 370 1076 849 Fragments arranged by size: 1076 849 370 BsaBI GATnn'nnATC Cuts at: 0 703 2295 Size: 703 1592 BsaHI Gr'CG_yC Cuts at: 0 75 176 2144 2156 2295 Size: 75 101 1968 12 139 Fragments arranged by size: 1968 139 101 75 12 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2180 2210 2295 Size: 2180 30 85 Fragments arranged by size: 2180 85 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1927 2019 2295 Size: 1927 92 276 Fragments arranged by size: 1927 276 92 BseYI C'CCAG_C Cuts at: 0 27 770 800 1283 2147 2295 Size: 27 743 30 483 864 148 Fragments arranged by size: 864 743 483 148 30 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 385 1279 1388 1576 1675 1873 1883 2160 Size: 385 894 109 188 99 198 10 277 Cuts at: 2160 2295 Size: 135 Fragments arranged by size: 894 385 277 198 188 135 109 99 10 BsiEI CG_ry'CG Cuts at: 0 1035 2295 Size: 1035 1260 BsiHKAI G_wGCw'C Cuts at: 0 1863 2011 2295 Size: 1863 148 284 Fragments arranged by size: 1863 284 148 Bsp1286I G_dGCh'C Cuts at: 0 1020 1236 1290 1863 2011 2295 Size: 1020 216 54 573 148 284 Fragments arranged by size: 1020 573 284 216 148 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1119 1472 1643 2295 Size: 1119 353 171 652 Fragments arranged by size: 1119 652 353 171 BsrBI CCG'CTC Cuts at: 0 1608 1651 1676 2295 Size: 1608 43 25 619 Fragments arranged by size: 1608 619 43 25 BsrGI T'GTAC_A Cuts at: 0 626 2295 Size: 626 1669 BssHII G'CGCG_C Cuts at: 0 1330 2295 Size: 1330 965 BssSI C'ACGA_G Cuts at: 0 1150 2295 Size: 1150 1145 BstAPI GCAn_nnn'nTGC Cuts at: 0 1252 2295 Size: 1252 1043 BstEII G'GTnAC_C Cuts at: 0 1086 2295 Size: 1086 1209 BstXI CCAn_nnnn'nTGG Cuts at: 0 507 1269 2295 Size: 507 762 1026 Fragments arranged by size: 1026 762 507 BtgI C'CryG_G Cuts at: 0 1347 1905 2295 Size: 1347 558 390 Fragments arranged by size: 1347 558 390 BtsI GCAGTG_nn' Cuts at: 0 868 1125 1291 1325 1588 1687 1694 1930 Size: 868 257 166 34 263 99 7 236 Cuts at: 1930 1945 2295 Size: 15 350 Fragments arranged by size: 868 350 263 257 236 166 99 34 15 7 DraIII CAC_nnn'GTG Cuts at: 0 249 1252 2295 Size: 249 1003 1043 Fragments arranged by size: 1043 1003 249 EaeI y'GGCC_r Cuts at: 0 83 302 386 757 911 1499 1512 2093 Size: 83 219 84 371 154 588 13 581 Cuts at: 2093 2252 2295 Size: 159 43 Fragments arranged by size: 588 581 371 219 159 154 84 83 43 13 EarI CTCTTCn'nnn_ Cuts at: 0 814 1397 1636 2295 Size: 814 583 239 659 Fragments arranged by size: 814 659 583 239 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 147 2295 Size: 147 2148 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 81 337 610 1006 1030 1660 1837 2295 Size: 81 256 273 396 24 630 177 458 Fragments arranged by size: 630 458 396 273 256 177 81 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 319 1468 2295 Size: 319 1149 827 Fragments arranged by size: 1149 827 319 EcoO109I rG'GnC_Cy Cuts at: 0 603 610 810 906 1017 1287 1352 2295 Size: 603 7 200 96 111 270 65 943 Fragments arranged by size: 943 603 270 200 111 96 65 7 HaeII r_GCGC'y Cuts at: 0 78 129 2147 2295 Size: 78 51 2018 148 Fragments arranged by size: 2018 148 78 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 979 1011 1812 1844 2295 Size: 979 32 801 32 451 Fragments arranged by size: 979 801 451 32 32 KasI G'GCGC_C Cuts at: 0 74 2143 2295 Size: 74 2069 152 Fragments arranged by size: 2069 152 74 MfeI C'AATT_G Cuts at: 0 638 2295 Size: 638 1657 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1055 1538 1653 2295 Size: 1055 483 115 642 Fragments arranged by size: 1055 642 483 115 MscI TGG'CCA Cuts at: 0 85 304 388 759 913 1514 2254 2295 Size: 85 219 84 371 154 601 740 41 Fragments arranged by size: 740 601 371 219 154 85 84 41 MslI CAynn'nnrTG Cuts at: 0 523 694 2048 2295 Size: 523 171 1354 247 Fragments arranged by size: 1354 523 247 171 MspA1I CmG'CkG Cuts at: 0 89 1702 2151 2295 Size: 89 1613 449 144 Fragments arranged by size: 1613 449 144 89 NarI GG'CG_CC Cuts at: 0 75 2144 2295 Size: 75 2069 151 Fragments arranged by size: 2069 151 75 NsiI A_TGCA'T Cuts at: 0 473 2295 Size: 473 1822 NspI r_CATG'y Cuts at: 0 471 475 561 726 2295 Size: 471 4 86 165 1569 Fragments arranged by size: 1569 471 165 86 4 PasI CC'CwG_GG Cuts at: 0 279 1113 2295 Size: 279 834 1182 Fragments arranged by size: 1182 834 279 PflMI CCAn_nnn'nTGG Cuts at: 0 777 1104 1833 2295 Size: 777 327 729 462 Fragments arranged by size: 777 729 462 327 PfoI T'CCnGG_A Cuts at: 0 455 587 1340 2159 2295 Size: 455 132 753 819 136 Fragments arranged by size: 819 753 455 136 132 PpuMI rG'GwC_Cy Cuts at: 0 610 810 2295 Size: 610 200 1485 Fragments arranged by size: 1485 610 200 PspOMI G'GGCC_C Cuts at: 0 1016 1286 2295 Size: 1016 270 1009 Fragments arranged by size: 1016 1009 270 PstI C_TGCA'G Cuts at: 0 878 1701 1940 1965 2295 Size: 878 823 239 25 330 Fragments arranged by size: 878 823 330 239 25 PvuII CAG'CTG Cuts at: 0 1702 2151 2295 Size: 1702 449 144 Fragments arranged by size: 1702 449 144 SfcI C'TryA_G Cuts at: 0 736 874 1697 1936 1961 2295 Size: 736 138 823 239 25 334 Fragments arranged by size: 823 736 334 239 138 25 SfoI GGC'GCC Cuts at: 0 76 2145 2295 Size: 76 2069 150 Fragments arranged by size: 2069 150 76 SmaI CCC'GGG Cuts at: 0 951 2295 Size: 951 1344 SmlI C'TyrA_G Cuts at: 0 436 1756 2101 2295 Size: 436 1320 345 194 Fragments arranged by size: 1320 436 345 194 SphI G_CATG'C Cuts at: 0 561 726 2295 Size: 561 165 1569 Fragments arranged by size: 1569 561 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 349 1783 1846 2251 2295 Size: 349 1434 63 405 44 Fragments arranged by size: 1434 405 349 63 44 TatI w'GTAC_w Cuts at: 0 530 626 2295 Size: 530 96 1669 Fragments arranged by size: 1669 530 96 XbaI T'CTAG_A Cuts at: 0 834 2295 Size: 834 1461 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 312 1177 2295 Size: 312 865 1118 Fragments arranged by size: 1118 865 312 XmaI C'CCGG_G Cuts at: 0 949 2295 Size: 949 1346 ZraI GAC'GTC Cuts at: 0 2157 2295 Size: 2157 138 Enzymes that do cut: AarI AatII AccI AcuI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrGI BssHII BssSI BstAPI BstEII BstXI BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I NarI NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SfcI SfoI SmaI SmlI SphI TaqII TatI XbaI XcmI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BclI BglII BmrI BmtI BsaAI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI StyI SwaI TstI Tth111I XhoI XmnI