################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AocI Aor51HI ApaI AscI AseI AsnI AspI AviII AvrII BbvCI BcgI BfrI BlnI BsaBI BsaI BsaWI BseAI BseRI BsiWI BsmBI Bsp1407I BspEI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I Cfr10I CpoI DrdI Eco47III Eco81I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 74 enzymes) # ################################### Acc65I 817 GGTACC G^GTACC AccII 163 CGCG CG^CG AcyI 11 GGCGCC G[GA]^CG[CT]C AflIII 479 ACGTGT A^C[GA][CT]GT AhdI 847 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 519 GTGCAC G^TGCAC ApaLI 519 GTGCAC G^TGCAC Asp700I 934 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 817 GGTACC G^GTACC AspEI 847 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 1120 GGACC G^G[AT]CC BalI 1020 TGGCCA TGG^CCA BamHI 129 GGATCC G^GATCC BbeI 11 GGCGCC GGCGC^C BbrPI 364 CACGTG CAC^GTG BclI 243 TGATCA T^GATCA BcnI 111 CCCGG CC^[GC]GG BglI 115 GCCGAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1031 AGATCT A^GATCT BlpI 1296 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1296 GCTGAGC GC^T[ACGT]AGC BsaAI 364 CACGTG [CT]AC^GT[GA] BsaHI 11 GGCGCC G[GA]^CG[CT]C BsiEI 113 CGGCCG CG[GA][CT]^CG BsmI 1250 GCATTC GAATGC(1/-1) BspDI 175 ATCGAT AT^CGAT BspHI 1170 TCATGA T^CATGA BspMI 97 ACCTGC ACCTGC(4/8) BsrBI 53 CCGCTC CCGCTC(-3/-3) BssSI 229 CACGAG CACGAG(-5/-1) BstAPI 153 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 637 GGTAACC G^GT[ACGT]ACC BstPI 637 GGTAACC G^GT[ACGT]ACC BstUI 163 CGCG CG^CG BstXI 133 CCACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1296 GCTGAGC GC^T[ACGT]AGC ClaI 175 ATCGAT AT^CGAT DraI 1576 TTTAAA TTT^AAA DraII 1790 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 1666 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 446 CCATGG C^C[GA][CT]GG EagI 113 CGGCCG C^GGCCG Eam1105I 847 GACTTTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 983 GAAGAG CTCTTC(1/4) EclXI 113 CGGCCG C^GGCCG Eco52I 113 CGGCCG C^GGCCG EcoNI 595 CCTTTTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1790 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 637 GGTAACC G^GT[ACGT]ACC FbaI 243 TGATCA T^GATCA HgaI 275 GACGC GACGC(5/10) Hin1I 11 GGCGCC G[GA]^CG[CT]C HindIII 1001 AAGCTT A^AGCTT KasI 11 GGCGCC G^GCGCC KpnI 817 GGTACC GGTAC^C Ksp632I 983 GAAGAG CTCTTC(1/4) MluNI 1020 TGGCCA TGG^CCA MscI 1020 TGGCCA TGG^CCA MvnI 163 CGCG CG^CG NarI 11 GGCGCC GG^CGCC NciI 111 CCCGG CC^[GC]GG NcoI 446 CCATGG C^CATGG NspI 1059 GCATGT [GA]CATG^[CT] PmaCI 364 CACGTG CAC^GTG PmlI 364 CACGTG CAC^GTG PstI 151 CTGCAG CTGCA^G RcaI 1170 TCATGA T^CATGA SacI 1287 GAGCTC GAGCT^C SfoI 11 GGCGCC GGC^GCC SmlI 970 CTCAAG C^T[CT][GA]AG SpeI 1423 ACTAGT A^CTAGT XbaI 1254 TCTAGA T^CTAGA XcmI 446 CCATGGCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 934 GAAGACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwNI 154 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1752 CAGTCTCTG AvaI 125 CTCGGG C^[CT]CG[GA]G 1676 CCCGAG BanI 11 GGCGCC G^G[CT][GA]CC 817 GGTACC BanII 463 GGGCTC G[GA]GC[CT]^C 1287 GAGCTC BciVI 483 GTATCC GTATCC(6/5) 1593 GTATCC BpmI 103 CTCCAG CTGGAG(16/14) 1509 CTGGAG BsoBI 125 CTCGGG C^[CT]CG[GA]G 1676 CCCGAG BsrDI 747 CATTGC GCAATG(2/0) 1277 CATTGC EaeI 113 CGGCCG [CT]^GGCC[GA] 1020 TGGCCA EcoT14I 446 CCATGG C^C[AT][AT]GG 1188 CCTTGG FauI 264 CCCGC CCCGC(4/6) 771 CCCGC HaeII 11 GGCGCC [GA]GCGC^[CT] 107 AGCGCC HapII 112 CCGG C^CGG 1282 CCGG HpaII 112 CCGG C^CGG 1282 CCGG HphI 581 TCACC GGTGA(8/7) 1796 TCACC MslI 707 CACCTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1852 CACCTATATG MspI 112 CCGG C^CGG 1282 CCGG SfaNI 1731 GCATC GCATC(5/9) 1813 GCATC SfcI 151 CTGCAG C^T[GA][CT]AG 1761 CTATAG StyI 446 CCATGG C^C[AT][AT]GG 1188 CCTTGG TaqI 176 TCGA T^CGA 1076 TCGA Tsp45I 845 GTGAC ^GT[GC]AC 1784 GTGAC TthHB8I 176 TCGA T^CGA 1076 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI BbsI BbvI BfaI BmyI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HinfI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI TfiI Tru9I TseI Tsp509I TspRI XhoII